(Circular) (Six-base) MAPSORT of: pFN21AB9098.seq Check: 5328 from: 1 to: 8476 pFN21AB9098 8476 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2448 2448 Size: 8476 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2862 6286 278 Size: 53 83 186 936 1326 3424 2468 Fragments arranged by size: 3424 2468 1326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5819 1937 Size: 3882 4594 Acc65I G'GTAC_C Cuts at: 5797 5797 Size: 8476 AclI AA'CG_TT Cuts at: 1563 6603 6976 1563 Size: 5040 373 3063 Fragments arranged by size: 5040 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 2546 2945 3104 3856 4315 4419 4560 Size: 652 262 399 159 752 459 104 141 Cuts at: 4560 5055 5223 6539 7923 8191 1632 Size: 495 168 1316 1384 268 1917 Fragments arranged by size: 1917 1384 1316 752 652 495 459 399 268 262 168 159 141 104 AfeI AGC'GCT Cuts at: 4440 4440 Size: 8476 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8279 AflIII A'CryG_T Cuts at: 1203 7375 8352 1203 Size: 6172 977 1327 Fragments arranged by size: 6172 1327 977 AgeI A'CCGG_T Cuts at: 7307 7307 Size: 8476 AhdI GACnn_n'nnGTC Cuts at: 4623 7205 4623 Size: 2582 5894 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3917 1184 Size: 2733 5743 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 8444 AlwNI CAG_nnn'CTG Cuts at: 2152 2779 3043 6219 7791 2152 Size: 627 264 3176 1572 2837 Fragments arranged by size: 3176 2837 1572 627 264 ApaLI G'TGCA_C Cuts at: 6533 7689 6533 Size: 1156 7320 ApoI r'AATT_y Cuts at: 1493 3799 4570 5785 6011 6163 8272 1493 Size: 2306 771 1215 226 152 2109 1697 Fragments arranged by size: 2306 2109 1697 1215 771 226 152 AseI AT'TA_AT Cuts at: 160 1032 2420 7030 160 Size: 872 1388 4610 1606 Fragments arranged by size: 4610 1606 1388 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8476 AvaI C'yCGr_G Cuts at: 1870 1943 2176 2191 5801 1870 Size: 73 233 15 3610 4545 Fragments arranged by size: 4545 3610 233 73 15 BamHI G'GATC_C Cuts at: 2016 3617 5806 6173 2016 Size: 1601 2189 367 4319 Fragments arranged by size: 4319 2189 1601 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 3435 4703 5797 7252 Size: 325 217 627 1613 35 1268 1094 1455 Cuts at: 7252 618 Size: 1842 Fragments arranged by size: 1842 1613 1455 1268 1094 627 325 217 35 BanII G_rGCy'C Cuts at: 1391 2183 3189 3777 4092 4457 4881 4932 5055 Size: 792 1006 588 315 365 424 51 123 Cuts at: 5055 5795 1391 Size: 740 4072 Fragments arranged by size: 4072 1006 792 740 588 424 365 315 123 51 BbeI G_GCGC'C Cuts at: 3439 3439 Size: 8476 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2471 2607 2678 3997 4434 5107 5589 Size: 971 572 136 71 1319 437 673 482 Cuts at: 5589 5736 928 Size: 147 3668 Fragments arranged by size: 3668 1319 971 673 572 482 437 147 136 71 BbvCI CC'TCA_GC Cuts at: 2330 2757 4280 2330 Size: 427 1523 6526 Fragments arranged by size: 6526 1523 427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3316 3350 4876 4910 6667 Size: 34 50 34 1295 34 1526 34 1757 Cuts at: 6667 6701 1903 Size: 34 3678 Fragments arranged by size: 3678 1757 1526 1295 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6368 7578 6368 Size: 1210 7266 BclI T'GATC_A Cuts at: 1548 3330 3825 4489 1548 Size: 1782 495 664 5535 Fragments arranged by size: 5535 1782 664 495 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2194 4658 7087 Size: 107 122 71 631 605 522 2464 2429 Cuts at: 7087 136 Size: 1525 Fragments arranged by size: 2464 2429 1525 631 605 522 122 107 71 BglII A'GATC_T Cuts at: 2907 8471 2907 Size: 5564 2912 BlpI GC'TnA_GC Cuts at: 4442 5049 5893 4442 Size: 607 844 7025 Fragments arranged by size: 7025 844 607 Bme1580I G_kGCm'C Cuts at: 1790 3405 4706 6537 7693 1790 Size: 1615 1301 1831 1156 2573 Fragments arranged by size: 2573 1831 1615 1301 1156 BmgBI CAC'GTC Cuts at: 1318 2038 5397 1318 Size: 720 3359 4397 Fragments arranged by size: 4397 3359 720 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4846 6207 7165 Size: 416 53 83 399 24 3429 1361 958 Cuts at: 7165 442 Size: 1753 Fragments arranged by size: 3429 1753 1361 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8476 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 5371 2191 Size: 3180 5296 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2291 2771 2923 3146 3272 3543 3956 Size: 989 63 480 152 223 126 271 413 Cuts at: 3956 4106 5033 5096 5366 7136 1239 Size: 150 927 63 270 1770 2579 Fragments arranged by size: 2579 1770 989 927 480 413 271 270 223 152 150 126 63 63 Bpu10I CC'TnA_GC Cuts at: 2090 2330 2757 3962 4280 4738 2090 Size: 240 427 1205 318 458 5828 Fragments arranged by size: 5828 1205 458 427 318 240 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 6601 7466 7764 2534 Size: 4067 865 298 3246 Fragments arranged by size: 4067 3246 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3122 3450 4859 7139 882 Size: 617 1623 328 1409 2280 2219 Fragments arranged by size: 2280 2219 1623 1409 617 328 BsaAI yAC'GTr Cuts at: 493 1204 2770 6267 493 Size: 711 1566 3497 2702 Fragments arranged by size: 3497 2702 1566 711 BsaBI GATnn'nnATC Cuts at: 2513 4488 8470 2513 Size: 1975 3982 2519 Fragments arranged by size: 3982 2519 1975 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2859 3436 3837 5567 Size: 53 83 186 936 1326 577 401 1730 Cuts at: 5567 6283 6665 7300 275 Size: 716 382 635 1451 Fragments arranged by size: 1730 1451 1326 936 716 635 577 401 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2694 3303 4725 6909 7307 7581 7728 8139 2694 Size: 609 1422 2184 398 274 147 411 3031 Fragments arranged by size: 3031 2184 1422 609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3245 3275 4079 4109 4522 Size: 30 165 30 1842 30 804 30 413 Cuts at: 4522 4552 5751 5781 1178 Size: 30 1199 30 3873 Fragments arranged by size: 3873 1842 1199 804 413 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3128 3269 3599 3617 4159 4490 5030 5150 Size: 1942 141 330 18 542 331 540 120 Cuts at: 5150 5573 1186 Size: 423 4089 Fragments arranged by size: 4089 1942 542 540 423 331 330 141 120 18 BseYI C'CCAG_C Cuts at: 7679 8175 7679 Size: 496 7980 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2651 2765 2792 3556 3974 4767 Size: 573 684 231 114 27 764 418 793 Cuts at: 4767 1163 Size: 4872 Fragments arranged by size: 4872 793 764 684 573 418 231 114 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2227 4662 5926 6687 7715 1540 Size: 275 186 226 2435 1264 761 1028 2301 Fragments arranged by size: 2435 2301 1264 1028 761 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2183 3189 3213 4092 4457 4830 4881 4932 5676 Size: 1006 24 879 365 373 51 51 744 Cuts at: 5676 6537 6622 7693 2183 Size: 861 85 1071 2966 Fragments arranged by size: 2966 1071 1006 879 861 744 373 365 85 51 51 24 BsmI GAATG_Cn' Cuts at: 5987 6080 5987 Size: 93 8383 BsmBI CGTCTCn'nnnn_ Cuts at: 3284 3284 Size: 8476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 3189 3213 3405 3777 4092 4457 Size: 399 393 1006 24 192 372 315 365 Cuts at: 4457 4706 4830 4881 4932 5055 5676 5795 6537 Size: 249 124 51 51 123 621 119 742 Cuts at: 6537 6622 7693 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1006 742 621 399 393 372 365 315 249 192 124 123 119 85 51 51 24 BspEI T'CCGG_A Cuts at: 3303 8139 3303 Size: 4836 3640 BspHI T'CATG_A Cuts at: 3205 3777 4614 5193 6365 8095 3205 Size: 572 837 579 1172 1730 3586 Fragments arranged by size: 3586 1730 1172 837 579 572 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2211 2442 2448 3960 5831 844 Size: 1316 51 231 6 1512 1871 3489 Fragments arranged by size: 3489 1871 1512 1316 231 51 6 BsrBI CCG'CTC Cuts at: 1394 1823 2521 3928 4945 6363 1394 Size: 429 698 1407 1017 1418 3507 Fragments arranged by size: 3507 1418 1407 1017 698 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2373 3775 5260 6971 7145 65 Size: 1183 1125 1402 1485 1711 174 1396 Fragments arranged by size: 1711 1485 1402 1396 1183 1125 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2245 2658 4215 7120 7307 1700 Size: 110 435 413 1557 2905 187 2869 Fragments arranged by size: 2905 2869 1557 435 413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8476 BssHII G'CGCG_C Cuts at: 1925 2060 2229 4150 1925 Size: 135 169 1921 6251 Fragments arranged by size: 6251 1921 169 135 BssSI C'ACGA_G Cuts at: 6536 7548 6536 Size: 1012 7464 BstAPI GCAn_nnn'nTGC Cuts at: 794 2152 2375 2443 2779 794 Size: 1358 223 68 336 6491 Fragments arranged by size: 6491 1358 336 223 68 BstBI TT'CG_AA Cuts at: 7280 7280 Size: 8476 BstEII G'GTnAC_C Cuts at: 1184 2697 1184 Size: 1513 6963 BstXI CCAn_nnnn'nTGG Cuts at: 4084 4772 4084 Size: 688 7788 BstYI r'GATC_y Cuts at: 1972 2016 2907 3039 3156 3348 3617 3906 5806 Size: 44 891 132 117 192 269 289 1900 Cuts at: 5806 6173 6558 6575 8027 8113 8125 8471 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1900 1452 891 385 367 346 289 269 192 132 117 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2469 513 Size: 552 1404 6520 Fragments arranged by size: 6520 1404 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7737 Fragments arranged by size: 7737 683 56 BtsI GCAGTG_nn' Cuts at: 3049 4654 5377 5992 6786 6806 3049 Size: 1605 723 615 794 20 4719 Fragments arranged by size: 4719 1605 794 723 615 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2111 2146 529 Size: 35 1547 35 6859 Fragments arranged by size: 6859 1547 35 35 DraI TTT'AAA Cuts at: 3649 5780 3649 Size: 2131 6345 DraIII CAC_nnn'GTG Cuts at: 4840 5513 4840 Size: 673 7803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5591 7483 809 Size: 507 4275 1892 1802 Fragments arranged by size: 4275 1892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2256 2472 Size: 54 1425 50 275 18 202 224 216 Cuts at: 2472 2820 3315 3622 4105 4272 4317 4659 5298 Size: 348 495 307 483 167 45 342 639 Cuts at: 5298 5923 6812 8441 8 Size: 625 889 1629 43 Fragments arranged by size: 1629 1425 889 639 625 495 483 348 342 307 275 224 216 202 167 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4659 5923 1537 Size: 275 2847 1264 4090 Fragments arranged by size: 4090 2847 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 6406 1353 Size: 809 4244 3423 Fragments arranged by size: 4244 3423 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2677 4234 4820 7063 7437 7583 Size: 1064 138 1088 1557 586 2243 374 146 Cuts at: 7583 387 Size: 1280 Fragments arranged by size: 2243 1557 1280 1088 1064 586 374 146 138 EcoICRI GAG'CTC Cuts at: 2181 3187 4090 4455 4879 4930 2181 Size: 1006 903 365 424 51 5727 Fragments arranged by size: 5727 1006 903 424 365 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2284 2291 2546 2771 2923 2945 Size: 393 596 56 7 255 225 152 22 Cuts at: 2945 3104 3146 3272 3543 3856 3956 4106 4315 Size: 159 42 126 271 313 100 150 209 Cuts at: 4315 4419 4560 5033 5055 5096 5223 5366 6539 Size: 104 141 473 22 41 127 143 1173 Cuts at: 6539 7136 7923 8191 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1173 787 597 596 473 393 313 271 268 255 225 209 159 152 150 143 141 127 126 104 100 56 42 41 22 22 7 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2549 3395 4937 2207 Size: 342 846 1542 5746 Fragments arranged by size: 5746 1542 846 342 EcoO109I rG'GnC_Cy Cuts at: 2394 4261 4987 5181 2394 Size: 1867 726 194 5689 Fragments arranged by size: 5689 1867 726 194 EcoRI G'AATT_C Cuts at: 5785 6163 5785 Size: 378 8098 EcoRV GAT'ATC Cuts at: 5619 5619 Size: 8476 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3553 5202 3553 Size: 1649 6827 FspI TGC'GCA Cuts at: 2720 6982 2720 Size: 4262 4214 HaeII r_GCGC'y Cuts at: 1678 2252 3439 4442 5283 7623 1678 Size: 574 1187 1003 841 2340 2531 Fragments arranged by size: 2531 2340 1187 1003 841 574 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2525 2557 3245 Size: 32 135 32 593 32 328 32 688 Cuts at: 3245 3277 4609 4641 7117 7149 7191 7223 1373 Size: 32 1332 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 1332 688 593 328 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2794 3862 5596 5820 6066 669 Size: 988 281 856 1068 1734 224 246 3079 Fragments arranged by size: 3079 1734 1068 988 856 281 246 224 HindIII A'AGCT_T Cuts at: 748 3530 5424 5447 748 Size: 2782 1894 23 3777 Fragments arranged by size: 3777 2782 1894 23 HpaI GTT'AAC Cuts at: 6066 6066 Size: 8476 KasI G'GCGC_C Cuts at: 3435 3435 Size: 8476 KpnI G_GTAC'C Cuts at: 5801 5801 Size: 8476 MfeI C'AATT_G Cuts at: 6075 6075 Size: 8476 MluI A'CGCG_T Cuts at: 8352 8352 Size: 8476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3858 4565 7590 7774 1305 Size: 2553 707 3025 184 2007 Fragments arranged by size: 3025 2553 2007 707 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2034 2474 3317 4107 10 Size: 54 1425 343 202 440 843 790 4379 Fragments arranged by size: 4379 1425 843 790 440 343 202 54 MslI CAynn'nnrTG Cuts at: 518 1166 3398 3695 4307 5027 5214 6434 6793 Size: 648 2232 297 612 720 187 1220 359 Cuts at: 6793 6952 518 Size: 159 2042 Fragments arranged by size: 2232 2042 1220 720 648 612 359 297 187 159 MspA1I CmG'CkG Cuts at: 1606 2269 2779 2812 2943 4284 5092 5319 5892 Size: 663 510 33 131 1341 808 227 573 Cuts at: 5892 6569 7717 7962 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1341 1148 808 677 663 573 510 245 227 131 33 NaeI GCC'GGC Cuts at: 2247 2660 4217 2247 Size: 413 1557 6506 Fragments arranged by size: 6506 1557 413 NarI GG'CG_CC Cuts at: 3436 3436 Size: 8476 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7924 NdeI CA'TA_TG Cuts at: 387 5312 387 Size: 4925 3551 NgoMIV G'CCGG_C Cuts at: 2245 2658 4215 2245 Size: 413 1557 6506 Fragments arranged by size: 6506 1557 413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8476 NotI GC'GGCC_GC Cuts at: 5923 5923 Size: 8476 NspI r_CATG'y Cuts at: 1132 1229 2384 2719 2749 4312 5834 7379 1132 Size: 97 1155 335 30 1563 1522 1545 2229 Fragments arranged by size: 2229 1563 1545 1522 1155 335 97 30 PasI CC'CwG_GG Cuts at: 1348 2047 1348 Size: 699 7777 PciI A'CATG_T Cuts at: 7375 7375 Size: 8476 PflMI CCAn_nnn'nTGG Cuts at: 1108 3751 1108 Size: 2643 5833 PfoI T'CCnGG_A Cuts at: 2682 3354 4026 4902 2682 Size: 672 672 876 6256 Fragments arranged by size: 6256 876 672 672 PmeI GTTT'AAAC Cuts at: 5780 5780 Size: 8476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3370 3402 5751 5783 6545 6577 8058 8090 3370 Size: 32 2349 32 762 32 1481 32 3756 Fragments arranged by size: 3756 2349 1481 762 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2394 4987 5181 2394 Size: 2593 194 5689 Fragments arranged by size: 5689 2593 194 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8476 PsiI TTA'TAA Cuts at: 6046 6046 Size: 8476 PspXI vC'TCGA_Gb Cuts at: 2176 2176 Size: 8476 PstI C_TGCA'G Cuts at: 830 2148 2448 3039 3906 4957 5828 830 Size: 1318 300 591 867 1051 871 3478 Fragments arranged by size: 3478 1318 1051 871 867 591 300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8476 PvuII CAG'CTG Cuts at: 2779 2943 4284 5092 2779 Size: 164 1341 808 6163 Fragments arranged by size: 6163 1341 808 164 SacI G_AGCT'C Cuts at: 2183 3189 4092 4457 4881 4932 2183 Size: 1006 903 365 424 51 5727 Fragments arranged by size: 5727 1006 903 424 365 51 SalI G'TCGA_C Cuts at: 1936 5818 1936 Size: 3882 4594 SapI GCTCTTCn'nnn_ Cuts at: 2162 2162 Size: 8476 SbfI CC_TGCA'GG Cuts at: 5828 5828 Size: 8476 ScaI AGT'ACT Cuts at: 6724 8436 6724 Size: 1712 6764 SexAI A'CCwGG_T Cuts at: 2730 2730 Size: 8476 SfcI C'TryA_G Cuts at: 826 1045 2144 2389 2444 3035 3902 4953 5824 Size: 219 1099 245 55 591 867 1051 871 Cuts at: 5824 6959 7640 7831 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1099 1051 871 867 681 591 245 219 191 55 SfoI GGC'GCC Cuts at: 3437 3437 Size: 8476 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8476 SgrAI Cr'CCGG_yG Cuts at: 1700 4215 1700 Size: 2515 5961 SmaI CCC'GGG Cuts at: 1872 5803 1872 Size: 3931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2176 2549 6580 7481 7743 820 Size: 197 926 233 373 4031 901 262 1553 Fragments arranged by size: 4031 1553 926 901 373 262 233 197 SnaBI TAC'GTA Cuts at: 493 6267 493 Size: 5774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8476 SphI G_CATG'C Cuts at: 1132 2719 2749 5834 1132 Size: 1587 30 3085 3774 Fragments arranged by size: 3774 3085 1587 30 SspI AAT'ATT Cuts at: 5 52 5696 6400 5 Size: 47 5644 704 2081 Fragments arranged by size: 5644 2081 704 47 StuI AGG'CCT Cuts at: 1517 2922 1517 Size: 1405 7071 StyI C'CwwG_G Cuts at: 513 1065 2259 2322 2917 3804 5915 513 Size: 552 1194 63 595 887 2111 3074 Fragments arranged by size: 3074 2111 1194 887 595 552 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2353 2591 4564 4828 5284 6503 6520 Size: 1255 773 238 1973 264 456 1219 17 Cuts at: 6520 6673 6858 7303 325 Size: 153 185 445 1498 Fragments arranged by size: 1973 1498 1255 1219 773 456 445 264 238 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3027 4311 6722 Size: 274 80 33 51 1443 1050 1284 2411 Cuts at: 6722 8434 96 Size: 1712 138 Fragments arranged by size: 2411 1712 1443 1284 1050 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 4826 4858 5499 5531 8436 8468 2027 Size: 32 2767 32 641 32 2905 32 2035 Fragments arranged by size: 2905 2767 2035 641 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2858 4201 1316 Size: 1542 1343 5591 Fragments arranged by size: 5591 1542 1343 XbaI T'CTAG_A Cuts at: 5812 5812 Size: 8476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2956 3247 3436 4018 4024 4505 2266 Size: 690 291 189 582 6 481 6237 Fragments arranged by size: 6237 690 582 481 291 189 6 XhoI C'TCGA_G Cuts at: 1943 2176 1943 Size: 233 8243 XmaI C'CCGG_G Cuts at: 1870 5801 1870 Size: 3931 4545 XmnI GAAnn'nnTTC Cuts at: 6605 6605 Size: 8476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2860 6284 276 Size: 53 83 186 936 1326 3424 2468 Fragments arranged by size: 3424 2468 1326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SrfI SwaI