Restriction map for
pFN21AB9055
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII AccI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AviII AvrII
BamHI BbeI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaHI BsiEI
BsiWI Bsp1407I BspDI BspHI BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI MfeI MluI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PflFI PflMI PinAI
PmeI PshAI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SgrAI Sse8387I SspBI
SwaI Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 399 GGTACC G^GTACC
AccIII 459 TCCGGA T^CCGGA
AclI 2071 AACGTT AA^CGTT
AlwNI 605 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2263 CCTGAGG CC^T[ACGT]AGG
AseI 2785 ATTAAT AT^TAAT
AsnI 2785 ATTAAT AT^TAAT
Asp718I 399 GGTACC G^GTACC
BalI 110 TGGCCA TGG^CCA
BanII 2406 GAGCCC G[GA]GC[CT]^C
BbvCI 2033 CCTCAGC CCTCAGC(-5/-2)
BclI 2802 TGATCA T^GATCA
BglI 124 GCCGAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1125 AGATCT A^GATCT
BsaBI 739 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 459 TCCGGA T^CCGGA
BsgI 448 GTGCAG GTGCAG(16/14)
BsmBI 2374 GAGACG CGTCTC(1/5)
BspEI 459 TCCGGA T^CCGGA
BspLU11I 831 ACATGT A^CATGT
BssSI 2087 CACGAG CACGAG(-5/-1)
BstBI 2042 TTCGAA TT^CGAA
BstEII 1995 GGTCACC G^GT[ACGT]ACC
BstPI 1995 GGTCACC G^GT[ACGT]ACC
BstXI 2693 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2263 CCTGAGG CC^T[ACGT]AGG
DraI 475 TTTAAA TTT^AAA
EaeI 110 TGGCCA [CT]^GGCC[GA]
Eco81I 2263 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1995 GGTCACC G^GT[ACGT]ACC
EcoT22I 268 ATGCAT ATGCA^T
FbaI 2802 TGATCA T^GATCA
KpnI 399 GGTACC GGTAC^C
KspI 1839 CCGCGG CCGC^GG
MamI 739 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 110 TGGCCA TGG^CCA
MroI 459 TCCGGA T^CCGGA
MscI 110 TGGCCA TGG^CCA
NdeI 410 CATATG CA^TATG
NsiI 268 ATGCAT ATGCA^T
NspV 2042 TTCGAA TT^CGAA
PaeR7I 88 CTCGAG C^TCGAG
PpuMI 854 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2785 ATTAAT AT^TAAT
Psp1406I 2071 AACGTT AA^CGTT
PstI 260 CTGCAG CTGCA^G
SacII 1839 CCGCGG CCGC^GG
SexAI 1804 ACCTGGT A^CC[AT]GGT
SfuI 2042 TTCGAA TT^CGAA
SmaI 2670 CCCGGG CCC^GGG
SnaBI 1022 TACGTA TAC^GTA
SpeI 1881 ACTAGT A^CTAGT
SphI 2663 GCATGC GCATG^C
SspI 233 AATATT AAT^ATT
StuI 2111 AGGCCT AGG^CCT
XhoI 88 CTCGAG C^TCGAG
XmaCI 2670 CCCGGG C^CCGGG
XmaI 2670 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 1840 CGCG CG^CG
2217 CGCG
AspHI 1389 GAGCAC G[AT]GC[AT]^C
2391 GAGCAC
BanI 399 GGTACC G^G[CT][GA]CC
2208 GGTGCC
BbrPI 1265 CACGTG CAC^GTG
2928 CACGTG
BsaWI 459 TCCGGA [AT]^CCGG[AT]
1083 TCCGGT
BseRI 2397 CTCCTC GAGGAG(10/8)
40 GAGGAG
BsiHKAI 1389 GAGCAC G[AT]GC[AT]^C
2391 GAGCAC
BstUI 1840 CGCG CG^CG
2217 CGCG
DrdI 157 GACGTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1952 GACCACGAAGTC
DsaI 1839 CCGCGG C^C[GA][CT]GG
2000 CCGTGG
EarI 2158 CTCTTC CTCTTC(1/4)
1666 GAAGAG
FauI 952 CCCGC CCCGC(4/6)
1841 GCGGG
HindIII 377 AAGCTT A^AGCTT
1931 AAGCTT
Ksp632I 2158 CTCTTC CTCTTC(1/4)
1666 GAAGAG
MvnI 1840 CGCG CG^CG
2217 CGCG
PmaCI 1265 CACGTG CAC^GTG
2928 CACGTG
PmlI 1265 CACGTG CAC^GTG
2928 CACGTG
XcmI 119 CCAAAGCCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2920 CCAGACCCCACGTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaI AvaII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI