Restriction map for
pFN21AB7011
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# NO CUT ENZYMES ( 78 enzymes) #
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AclI AflII AgeI AocI AscI
AseI AsnI AviII AvrII BamHI
BcgI BfrI BglII BlnI BsaBI
BsaI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I ClaI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NsiI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI SalI SbfI
ScaI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2350 GACGTC GACGT^C
Acc65I 398 GGTACC G^GTACC
AccIII 1112 TCCGGA T^CCGGA
Alw44I 2734 GTGCAC G^TGCAC
Aor51HI 821 AGCGCT AGC^GCT
ApaI 44 GGGCCC GGGCC^C
ApaLI 2734 GTGCAC G^TGCAC
Asp700I 1599 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 398 GGTACC G^GTACC
AspI 1488 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1335 GGCGCC GGCGC^C
BbsI 2446 GAAGAC GAAGAC(2/6)
BciVI 867 GGATAC GTATCC(6/5)
BlpI 804 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 804 GCTGAGC GC^T[ACGT]AGC
BpuAI 2446 GAAGAC GAAGAC(2/6)
BseAI 1112 TCCGGA T^CCGGA
BsmBI 2763 CGTCTC CGTCTC(1/5)
BspEI 1112 TCCGGA T^CCGGA
BstBI 3049 TTCGAA TT^CGAA
CelII 804 GCTGAGC GC^T[ACGT]AGC
CpoI 2521 CGGTCCG CG^G[AT]CCG
DraI 2395 TTTAAA TTT^AAA
Eco47III 821 AGCGCT AGC^GCT
HincII 444 GTTGAC GT[CT]^[GA]AC
HindII 444 GTTGAC GT[CT]^[GA]AC
KasI 1335 GGCGCC G^GCGCC
KpnI 398 GGTACC GGTAC^C
MroI 1112 TCCGGA T^CCGGA
NaeI 1226 GCCGGC GCC^GGC
NarI 1335 GGCGCC GG^CGCC
NcoI 2768 CCATGG C^CATGG
NgoMIV 1226 GCCGGC G^CCGGC
NruI 3094 TCGCGA TCG^CGA
NspI 2330 GCATGT [GA]CATG^[CT]
NspV 3049 TTCGAA TT^CGAA
PaeR7I 247 CTCGAG C^TCGAG
PflFI 1488 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 2521 CGGTCCG CG^G[AT]CCG
SexAI 2078 ACCAGGT A^CC[AT]GGT
SfiI 1938 GGCCTTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1335 GGCGCC GGC^GCC
SfuI 3049 TTCGAA TT^CGAA
Tth111I 1488 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 247 CTCGAG C^TCGAG
XmnI 1599 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 1644 GTCTAC GT^[AC][GT]AC
3016 GTCTAC
BbrPI 2737 CACGTG CAC^GTG
3103 CACGTG
BsaWI 680 TCCGGT [AT]^CCGG[AT]
1112 TCCGGA
BsrFI 1226 GCCGGC [GA]^CCGG[CT]
2060 ACCGGC
BstXI 2498 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2561 CCATTGACCTGG
BstYI 285 GGATCT [GA]^GATC[CT]
1671 GGATCT
Cfr10I 1226 GCCGGC [GA]^CCGG[CT]
2060 ACCGGC
MflI 285 GGATCT [GA]^GATC[CT]
1671 GGATCT
PflMI 132 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2079 CCAGGTCCTGG
PmaCI 2737 CACGTG CAC^GTG
3103 CACGTG
PmlI 2737 CACGTG CAC^GTG
3103 CACGTG
PpuMI 1372 AGGACCC [GA]G^G[AT]CC[CT]
2081 AGGTCCT
SacI 176 GAGCTC GAGCT^C
1968 GAGCTC
SapI 958 GAAGAGC GCTCTTC(1/4)
2577 GAAGAGC
Van91I 132 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2079 CCAGGTCCTGG
XcmI 600 CCATAACCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2759 CCAGCGTCTCCATGG
XhoII 285 GGATCT [GA]^GATC[CT]
1671 GGATCT
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I