(Circular) (Six-base) MAPSORT of: pFN21AB7011.seq Check: 8653 from: 1 to: 7840 pFN21AB7011 7840 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2410 2410 Size: 7840 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4359 5650 278 Size: 53 83 186 936 2823 1291 2468 Fragments arranged by size: 2823 2468 1291 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 5022 5183 1937 Size: 1713 1372 161 4594 Fragments arranged by size: 4594 1713 1372 161 Acc65I G'GTAC_C Cuts at: 2403 5161 2403 Size: 2758 5082 AclI AA'CG_TT Cuts at: 1563 5967 6340 1563 Size: 4404 373 3063 Fragments arranged by size: 4404 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 3692 3908 4117 4768 5014 5903 7287 Size: 462 1598 216 209 651 246 889 1384 Cuts at: 7287 7555 1632 Size: 268 1917 Fragments arranged by size: 1917 1598 1384 889 651 462 268 246 216 209 AfeI AGC'GCT Cuts at: 2828 2828 Size: 7840 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7643 AflIII A'CryG_T Cuts at: 1203 2453 4743 4992 5049 5109 6739 7716 1203 Size: 1250 2290 249 57 60 1630 977 1327 Fragments arranged by size: 2290 1630 1327 1250 977 249 60 57 AgeI A'CCGG_T Cuts at: 6671 6671 Size: 7840 AhdI GACnn_n'nnGTC Cuts at: 3432 4633 4666 6569 3432 Size: 1201 33 1903 4703 Fragments arranged by size: 4703 1903 1201 33 AleI CACnn'nnGTG Cuts at: 1166 4736 1166 Size: 3570 4270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3837 1184 Size: 2653 5187 AlwNI CAG_nnn'CTG Cuts at: 2143 4090 4140 4222 4858 5583 7155 2143 Size: 1947 50 82 636 725 1572 2828 Fragments arranged by size: 2828 1947 1572 725 636 82 50 ApaI G_GGCC'C Cuts at: 2053 2053 Size: 7840 ApaLI G'TGCA_C Cuts at: 4739 5897 7053 4739 Size: 1158 1156 5526 Fragments arranged by size: 5526 1158 1156 ApoI r'AATT_y Cuts at: 1493 2059 2357 2597 2748 2882 3148 3763 3782 Size: 566 298 240 151 134 266 615 19 Cuts at: 3782 4210 4829 5149 5375 5527 7636 1493 Size: 428 619 320 226 152 2109 1697 Fragments arranged by size: 2109 1697 619 615 566 428 320 298 266 240 226 152 151 134 19 AseI AT'TA_AT Cuts at: 160 1032 6394 160 Size: 872 5362 1606 Fragments arranged by size: 5362 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7840 AvaI C'yCGr_G Cuts at: 1870 1943 2252 3280 4312 5165 1870 Size: 73 309 1028 1032 853 4545 Fragments arranged by size: 4545 1032 1028 853 309 73 BamHI G'GATC_C Cuts at: 5170 5537 5170 Size: 367 7473 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2403 3203 3340 4465 4752 Size: 325 217 627 616 800 137 1125 287 Cuts at: 4752 5161 6616 618 Size: 409 1455 1842 Fragments arranged by size: 1842 1455 1125 800 627 616 409 325 287 217 137 BanII G_rGCy'C Cuts at: 1391 2053 2185 2970 3287 3515 3977 4326 5159 Size: 662 132 785 317 228 462 349 833 Cuts at: 5159 1391 Size: 4072 Fragments arranged by size: 4072 833 785 662 462 349 317 228 132 BbeI G_GCGC'C Cuts at: 3344 3344 Size: 7840 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4458 928 Size: 971 2559 4310 Fragments arranged by size: 4310 2559 971 BbvCI CC'TCA_GC Cuts at: 2565 3396 4301 4823 2565 Size: 831 905 522 5582 Fragments arranged by size: 5582 905 831 522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6031 6065 1903 Size: 34 4094 34 3678 Fragments arranged by size: 4094 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2866 5732 6942 2866 Size: 2866 1210 3764 Fragments arranged by size: 3764 2866 1210 BclI T'GATC_A Cuts at: 1548 2263 3540 4197 1548 Size: 715 1277 657 5191 Fragments arranged by size: 5191 1277 715 657 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3338 3950 4154 Size: 107 122 71 631 605 1666 612 204 Cuts at: 4154 4655 6451 136 Size: 501 1796 1525 Fragments arranged by size: 1796 1666 1525 631 612 605 501 204 122 107 71 BglII A'GATC_T Cuts at: 7835 7835 Size: 7840 BlpI GC'TnA_GC Cuts at: 2810 5257 2810 Size: 2447 5393 Bme1580I G_kGCm'C Cuts at: 1790 2053 4470 4743 5901 7057 1790 Size: 263 2417 273 1158 1156 2573 Fragments arranged by size: 2573 2417 1158 1156 273 263 BmgBI CAC'GTC Cuts at: 1318 2454 5020 1318 Size: 1136 2566 4138 Fragments arranged by size: 4138 2566 1136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2054 2800 5571 Size: 416 53 83 399 24 637 746 2771 Cuts at: 5571 6529 442 Size: 958 1753 Fragments arranged by size: 2771 1753 958 746 637 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7840 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3411 3411 Size: 7840 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3182 3198 3977 4007 4112 4310 4724 6500 Size: 1943 16 779 30 105 198 414 1776 Cuts at: 6500 1239 Size: 2579 Fragments arranged by size: 2579 1943 1776 779 414 198 105 30 16 Bpu10I CC'TnA_GC Cuts at: 2565 3396 3509 3639 4301 4682 4823 2565 Size: 831 113 130 662 381 141 5582 Fragments arranged by size: 5582 831 662 381 141 130 113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2424 3785 4217 5965 6830 7128 2424 Size: 1361 432 1748 865 298 3136 Fragments arranged by size: 3136 1748 1361 865 432 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6503 882 Size: 617 5004 2219 Fragments arranged by size: 5004 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4744 4993 5110 5631 493 Size: 711 3540 249 117 521 2702 Fragments arranged by size: 3540 2702 711 521 249 117 BsaBI GATnn'nnATC Cuts at: 7834 7834 Size: 7840 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3341 3726 4356 5647 Size: 53 83 186 936 1808 385 630 1291 Cuts at: 5647 6029 6664 275 Size: 382 635 1451 Fragments arranged by size: 1808 1451 1291 936 635 630 385 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2685 3117 6273 6671 6945 7092 7503 2685 Size: 432 3156 398 274 147 411 3022 Fragments arranged by size: 3156 3022 432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3257 3287 3484 3514 4085 Size: 30 165 30 1854 30 197 30 571 Cuts at: 4085 4115 4790 4820 1178 Size: 30 675 30 4198 Fragments arranged by size: 4198 1854 675 571 197 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3310 3371 3374 4457 1186 Size: 2124 61 3 1083 4569 Fragments arranged by size: 4569 2124 1083 61 3 BseYI C'CCAG_C Cuts at: 3381 7043 7539 3381 Size: 3662 496 3682 Fragments arranged by size: 3682 3662 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3101 4046 4208 4988 1163 Size: 573 1365 945 162 780 4015 Fragments arranged by size: 4015 1365 945 780 573 162 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5290 6051 7079 1540 Size: 275 186 3289 761 1028 2301 Fragments arranged by size: 3289 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2185 2541 3182 3710 3977 4743 5901 5986 7057 Size: 356 641 528 267 766 1158 85 1071 Cuts at: 7057 2185 Size: 2968 Fragments arranged by size: 2968 1158 1071 766 641 528 356 267 85 BsmI GAATG_Cn' Cuts at: 5351 5444 5351 Size: 93 7747 BsmBI CGTCTCn'nnnn_ Cuts at: 4774 4774 Size: 7840 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2185 2541 2970 3182 3287 3515 Size: 399 263 132 356 429 212 105 228 Cuts at: 3515 3710 3977 4326 4470 4743 5159 5901 5986 Size: 195 267 349 144 273 416 742 85 Cuts at: 5986 7057 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 429 416 399 356 349 273 267 263 228 212 195 144 132 105 85 BspEI T'CCGG_A Cuts at: 3117 7503 3117 Size: 4386 3454 BspHI T'CATG_A Cuts at: 5729 7459 5729 Size: 1730 6110 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2410 2436 3031 4827 5195 844 Size: 1566 26 595 1796 368 3489 Fragments arranged by size: 3489 1796 1566 595 368 26 BsrBI CCG'CTC Cuts at: 1394 1823 3840 4036 4096 4423 5727 1394 Size: 429 2017 196 60 327 1304 3507 Fragments arranged by size: 3507 2017 1304 429 327 196 60 BsrDI GCAATG_nn' Cuts at: 65 1248 6335 6509 65 Size: 1183 5087 174 1396 Fragments arranged by size: 5087 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3231 4065 6484 6671 1700 Size: 110 1421 834 2419 187 2869 Fragments arranged by size: 2869 2419 1421 834 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7840 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7840 BssSI C'ACGA_G Cuts at: 2120 2228 4369 5900 6912 2120 Size: 108 2141 1531 1012 3048 Fragments arranged by size: 3048 2141 1531 1012 108 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7840 BstBI TT'CG_AA Cuts at: 5055 6644 5055 Size: 1589 6251 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7840 BstXI CCAn_nnnn'nTGG Cuts at: 4510 4573 4510 Size: 63 7777 BstYI r'GATC_y Cuts at: 1972 2290 3676 5170 5537 5922 5939 7391 7477 Size: 318 1386 1494 367 385 17 1452 86 Cuts at: 7477 7489 7835 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1494 1452 1386 385 367 346 318 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3306 3345 4186 4408 4653 4723 Size: 552 939 1302 39 841 222 245 70 Cuts at: 4723 4773 513 Size: 50 3580 Fragments arranged by size: 3580 1302 939 841 552 245 222 70 50 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3891 4683 4774 487 Size: 683 56 2665 792 91 3553 Fragments arranged by size: 3553 2665 792 683 91 56 BtsI GCAGTG_nn' Cuts at: 4058 4471 4800 5086 5356 6150 6170 4058 Size: 413 329 286 270 794 20 5728 Fragments arranged by size: 5728 794 413 329 286 270 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7840 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7805 DraI TTT'AAA Cuts at: 4402 5144 4402 Size: 742 7098 DraIII CAC_nnn'GTG Cuts at: 2283 2494 3696 2283 Size: 211 1202 6427 Fragments arranged by size: 6427 1202 211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6847 809 Size: 507 5531 1802 Fragments arranged by size: 5531 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2697 2730 2846 Size: 54 1425 50 275 18 867 33 116 Cuts at: 2846 2892 4055 4512 4563 4611 5287 6176 7805 Size: 46 1163 457 51 48 676 889 1629 Cuts at: 7805 8 Size: 43 Fragments arranged by size: 1629 1425 1163 889 867 676 457 275 116 54 51 50 48 46 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 5287 1537 Size: 275 3475 4090 Fragments arranged by size: 4090 3475 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2958 3348 3599 4577 5770 1353 Size: 1605 390 251 978 1193 3423 Fragments arranged by size: 3423 1605 1193 978 390 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2355 3247 3338 4592 5051 6427 Size: 1064 138 766 892 91 1254 459 1376 Cuts at: 6427 6801 6947 387 Size: 374 146 1280 Fragments arranged by size: 1376 1280 1254 1064 892 766 459 374 146 138 91 EcoICRI GAG'CTC Cuts at: 2183 3975 2183 Size: 1792 6048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 3182 3198 3692 3908 3977 4007 Size: 393 462 1088 16 494 216 69 30 Cuts at: 4007 4112 4117 4310 4724 4768 5014 5903 6500 Size: 105 5 193 414 44 246 889 597 Cuts at: 6500 7287 7555 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1088 889 787 597 494 462 414 393 268 246 216 193 105 69 44 30 16 5 EcoO109I rG'GnC_Cy Cuts at: 2049 2050 3378 3952 4087 4714 2049 Size: 1 1328 574 135 627 5175 Fragments arranged by size: 5175 1328 627 574 135 1 EcoRI G'AATT_C Cuts at: 2748 2882 3148 5149 5527 2748 Size: 134 266 2001 378 5061 Fragments arranged by size: 5061 2001 378 266 134 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2449 2830 5023 5147 2449 Size: 381 2193 124 5142 Fragments arranged by size: 5142 2193 381 124 FspI TGC'GCA Cuts at: 6346 6346 Size: 7840 HaeII r_GCGC'y Cuts at: 1678 2830 3344 4689 4711 6987 1678 Size: 1152 514 1345 22 2276 2531 Fragments arranged by size: 2531 2276 1345 1152 514 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2647 2679 2881 2913 3257 Size: 32 135 32 1075 32 202 32 344 Cuts at: 3257 3289 3418 3450 3484 3516 4619 4651 4652 Size: 32 129 32 34 32 1103 32 1 Cuts at: 4652 4684 6481 6513 6555 6587 1373 Size: 32 1797 32 42 32 2626 Fragments arranged by size: 2626 1797 1103 1075 344 202 135 129 42 34 32 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2451 5184 5430 669 Size: 988 281 513 2733 246 3079 Fragments arranged by size: 3079 2733 988 513 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7840 HpaI GTT'AAC Cuts at: 5430 5430 Size: 7840 KasI G'GCGC_C Cuts at: 3340 3340 Size: 7840 KpnI G_GTAC'C Cuts at: 2407 5165 2407 Size: 2758 5082 MfeI C'AATT_G Cuts at: 5439 5439 Size: 7840 MluI A'CGCG_T Cuts at: 7716 7716 Size: 7840 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3008 6954 7138 1305 Size: 1703 3946 184 2007 Fragments arranged by size: 3946 2007 1703 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2894 4565 4613 10 Size: 54 1425 343 1062 1671 48 3237 Fragments arranged by size: 3237 1671 1425 1062 343 54 48 MslI CAynn'nnrTG Cuts at: 518 1166 4169 4508 4559 4736 4793 5798 6157 Size: 648 3003 339 51 177 57 1005 359 Cuts at: 6157 6316 518 Size: 159 2042 Fragments arranged by size: 3003 2042 1005 648 359 339 177 159 57 51 MspA1I CmG'CkG Cuts at: 1606 3308 3550 4495 4655 4725 5256 5933 7081 Size: 1702 242 945 160 70 531 677 1148 Cuts at: 7081 7326 1606 Size: 245 2120 Fragments arranged by size: 2120 1702 1148 945 677 531 245 242 160 70 NaeI GCC'GGC Cuts at: 3233 3233 Size: 7840 NarI GG'CG_CC Cuts at: 3341 3341 Size: 7840 NcoI C'CATG_G Cuts at: 513 1065 2004 4773 513 Size: 552 939 2769 3580 Fragments arranged by size: 3580 2769 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7840 NgoMIV G'CCGG_C Cuts at: 3231 3231 Size: 7840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7840 NotI GC'GGCC_GC Cuts at: 5287 5287 Size: 7840 NruI TCG'CGA Cuts at: 5101 5101 Size: 7840 NspI r_CATG'y Cuts at: 1132 1229 4339 5198 6743 1132 Size: 97 3110 859 1545 2229 Fragments arranged by size: 3110 2229 1545 859 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7840 PciI A'CATG_T Cuts at: 6739 6739 Size: 7840 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 4090 1108 Size: 1035 1947 4858 Fragments arranged by size: 4858 1947 1035 PfoI T'CCnGG_A Cuts at: 4089 4270 4089 Size: 181 7659 PmeI GTTT'AAAC Cuts at: 5144 5144 Size: 7840 PmlI CAC'GTG Cuts at: 4744 5110 4744 Size: 366 7474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 5909 5941 7422 7454 5036 Size: 32 841 32 1481 32 5422 Fragments arranged by size: 5422 1481 841 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3378 4087 3378 Size: 709 7131 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7840 PsiI TTA'TAA Cuts at: 5410 5410 Size: 7840 PspOMI G'GGCC_C Cuts at: 2049 2049 Size: 7840 PspXI vC'TCGA_Gb Cuts at: 2252 2252 Size: 7840 PstI C_TGCA'G Cuts at: 830 2421 4001 4017 5192 830 Size: 1591 1580 16 1175 3478 Fragments arranged by size: 3478 1591 1580 1175 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7840 RsrII CG'GwC_CG Cuts at: 4527 4527 Size: 7840 SacI G_AGCT'C Cuts at: 2185 3977 2185 Size: 1792 6048 SacII CC_GC'GG Cuts at: 3309 4656 4726 3309 Size: 1347 70 6423 Fragments arranged by size: 6423 1347 70 SalI G'TCGA_C Cuts at: 1936 5182 1936 Size: 3246 4594 SapI GCTCTTCn'nnn_ Cuts at: 2958 4577 2958 Size: 1619 6221 SbfI CC_TGCA'GG Cuts at: 5192 5192 Size: 7840 ScaI AGT'ACT Cuts at: 6088 7800 6088 Size: 1712 6128 SexAI A'CCwGG_T Cuts at: 4083 4083 Size: 7840 SfcI C'TryA_G Cuts at: 826 1045 2417 3138 3997 4013 5188 6323 7004 Size: 219 1372 721 859 16 1175 1135 681 Cuts at: 7004 7195 826 Size: 191 1471 Fragments arranged by size: 1471 1372 1175 1135 859 721 681 219 191 16 SfiI GGCCn_nnn'nGGCC Cuts at: 3950 3950 Size: 7840 SfoI GGC'GCC Cuts at: 3342 3342 Size: 7840 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7840 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7840 SmaI CCC'GGG Cuts at: 1872 5167 1872 Size: 3295 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2439 3800 4232 5944 6845 Size: 197 926 309 187 1361 432 1712 901 Cuts at: 6845 7107 820 Size: 262 1553 Fragments arranged by size: 1712 1553 1361 926 901 432 309 262 197 187 SnaBI TAC'GTA Cuts at: 493 5631 493 Size: 5138 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7840 SphI G_CATG'C Cuts at: 1132 5198 1132 Size: 4066 3774 SspI AAT'ATT Cuts at: 5 52 5764 5 Size: 47 5712 2081 Fragments arranged by size: 5712 2081 47 StuI AGG'CCT Cuts at: 1517 3530 3566 3944 1517 Size: 2013 36 378 5413 Fragments arranged by size: 5413 2013 378 36 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2526 2700 2796 2877 3945 Size: 552 939 72 450 174 96 81 1068 Cuts at: 3945 4773 5279 513 Size: 828 506 3074 Fragments arranged by size: 3074 1068 939 828 552 506 450 174 96 81 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 4515 4852 5867 5884 6037 6222 Size: 1255 588 2347 337 1015 17 153 185 Cuts at: 6222 6667 325 Size: 445 1498 Fragments arranged by size: 2347 1498 1255 1015 588 445 337 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5046 6086 7798 Size: 274 80 33 51 1443 3069 1040 1712 Cuts at: 7798 96 Size: 138 Fragments arranged by size: 3069 1712 1443 1040 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2106 2138 2739 2771 2914 2946 4085 4117 4997 Size: 32 601 32 143 32 1139 32 880 Cuts at: 4997 5029 7800 7832 2106 Size: 32 2771 32 2114 Fragments arranged by size: 2771 2114 1139 880 601 143 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3496 1316 Size: 2180 5660 XbaI T'CTAG_A Cuts at: 5176 5176 Size: 7840 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2612 4771 2612 Size: 2159 5681 XhoI C'TCGA_G Cuts at: 1943 2252 1943 Size: 309 7531 XmaI C'CCGG_G Cuts at: 1870 5165 1870 Size: 3295 4545 XmnI GAAnn'nnTTC Cuts at: 3608 5969 3608 Size: 2361 5479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4357 5648 276 Size: 53 83 186 936 2823 1291 2468 Fragments arranged by size: 2823 2468 1291 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NsiI PacI PsrI PvuII SanDI SrfI SwaI