Restriction map for
pFN21AB7011
(Linear) (Six-base) MAPSORT of: KIBB7011.seq Check: 150 from: 1 to: 3135
KIBB7011.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 405 3135
Size: 405 2730
AatII G_ACGT'C
Cuts at: 0 2354 3135
Size: 2354 781
AccI GT'mk_AC
Cuts at: 0 1645 3017 3135
Size: 1645 1372 118
Fragments arranged by size:
1645 1372 118
Acc65I G'GTAC_C
Cuts at: 0 398 3135
Size: 398 2737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1687 1903 2112 2763 3009 3135
Size: 89 1598 216 209 651 246 126
Fragments arranged by size:
1598 651 246 216 209 126 89
AfeI AGC'GCT
Cuts at: 0 823 3135
Size: 823 2312
AflIII A'CryG_T
Cuts at: 0 448 2738 2987 3044 3104 3135
Size: 448 2290 249 57 60 31
Fragments arranged by size:
2290 448 249 60 57 31
AhdI GACnn_n'nnGTC
Cuts at: 0 1427 2628 2661 3135
Size: 1427 1201 33 474
Fragments arranged by size:
1427 1201 474 33
AleI CACnn'nnGTG
Cuts at: 0 2731 3135
Size: 2731 404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1832 3135
Size: 1832 1303
AlwNI CAG_nnn'CTG
Cuts at: 0 138 2085 2135 2217 2853 3135
Size: 138 1947 50 82 636 282
Fragments arranged by size:
1947 636 282 138 82 50
ApaI G_GGCC'C
Cuts at: 0 48 3135
Size: 48 3087
ApaLI G'TGCA_C
Cuts at: 0 2734 3135
Size: 2734 401
ApoI r'AATT_y
Cuts at: 0 54 352 592 743 877 1143 1758 1777
Size: 54 298 240 151 134 266 615 19
Cuts at: 1777 2205 2824 3135
Size: 428 619 311
Fragments arranged by size:
619 615 428 311 298 266 240 151
134 54 19
AvaI C'yCGr_G
Cuts at: 0 247 1275 2307 3135
Size: 247 1028 1032 828
Fragments arranged by size:
1032 1028 828 247
BanI G'GyrC_C
Cuts at: 0 398 1198 1335 2460 2747 3135
Size: 398 800 137 1125 287 388
Fragments arranged by size:
1125 800 398 388 287 137
BanII G_rGCy'C
Cuts at: 0 48 180 965 1282 1510 1972 2321 3135
Size: 48 132 785 317 228 462 349 814
Fragments arranged by size:
814 785 462 349 317 228 132 48
BbeI G_GCGC'C
Cuts at: 0 1339 3135
Size: 1339 1796
BbsI GAAGACnn'nnnn_
Cuts at: 0 2453 3135
Size: 2453 682
BbvCI CC'TCA_GC
Cuts at: 0 560 1391 2296 2818 3135
Size: 560 831 905 522 317
Fragments arranged by size:
905 831 560 522 317
BciVI GTATCCnnnnn_n'
Cuts at: 0 861 3135
Size: 861 2274
BclI T'GATC_A
Cuts at: 0 258 1535 2192 3135
Size: 258 1277 657 943
Fragments arranged by size:
1277 943 657 258
BglI GCCn_nnn'nGGC
Cuts at: 0 1333 1945 2149 2650 3135
Size: 1333 612 204 501 485
Fragments arranged by size:
1333 612 501 485 204
BlpI GC'TnA_GC
Cuts at: 0 805 3135
Size: 805 2330
Bme1580I G_kGCm'C
Cuts at: 0 48 2465 2738 3135
Size: 48 2417 273 397
Fragments arranged by size:
2417 397 273 48
BmgBI CAC'GTC
Cuts at: 0 449 3015 3135
Size: 449 2566 120
Fragments arranged by size:
2566 449 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 49 795 3135
Size: 49 746 2340
Fragments arranged by size:
2340 746 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1406 3135
Size: 1406 1729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1193 1972 2002 2107 2305 2719 3135
Size: 1177 16 779 30 105 198 414 416
Fragments arranged by size:
1177 779 416 414 198 105 30 16
Bpu10I CC'TnA_GC
Cuts at: 0 560 1391 1504 1634 2296 2677 2818 3135
Size: 560 831 113 130 662 381 141 317
Fragments arranged by size:
831 662 560 381 317 141 130 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1780 2212 3135
Size: 419 1361 432 923
Fragments arranged by size:
1361 923 432 419
BsaAI yAC'GTr
Cuts at: 0 2739 2988 3105 3135
Size: 2739 249 117 30
Fragments arranged by size:
2739 249 117 30
BsaHI Gr'CG_yC
Cuts at: 0 1336 1721 2351 3135
Size: 1336 385 630 784
Fragments arranged by size:
1336 784 630 385
BsaWI w'CCGG_w
Cuts at: 0 680 1112 3135
Size: 680 432 2023
Fragments arranged by size:
2023 680 432
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1252 1282 1479 1509 2080 2110 2785 2815
Size: 1252 30 197 30 571 30 675 30
Cuts at: 2815 3135
Size: 320
Fragments arranged by size:
1252 675 571 320 197 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1305 1366 1369 2452 3135
Size: 1305 61 3 1083 683
Fragments arranged by size:
1305 1083 683 61 3
BseYI C'CCAG_C
Cuts at: 0 1376 3135
Size: 1376 1759
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 2041 2203 2983 3135
Size: 1096 945 162 780 152
Fragments arranged by size:
1096 945 780 162 152
BsiHKAI G_wGCw'C
Cuts at: 0 180 536 1177 1705 1972 2738 3135
Size: 180 356 641 528 267 766 397
Fragments arranged by size:
766 641 528 397 356 267 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2769 3135
Size: 2769 366
Bsp1286I G_dGCh'C
Cuts at: 0 48 180 536 965 1177 1282 1510 1705
Size: 48 132 356 429 212 105 228 195
Cuts at: 1705 1972 2321 2465 2738 3135
Size: 267 349 144 273 397
Fragments arranged by size:
429 397 356 349 273 267 228 212
195 144 132 105 48
BspEI T'CCGG_A
Cuts at: 0 1112 3135
Size: 1112 2023
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 405 431 1026 2822 3135
Size: 405 26 595 1796 313
Fragments arranged by size:
1796 595 405 313 26
BsrBI CCG'CTC
Cuts at: 0 1835 2031 2091 2418 3135
Size: 1835 196 60 327 717
Fragments arranged by size:
1835 717 327 196 60
BsrFI r'CCGG_y
Cuts at: 0 1226 2060 3135
Size: 1226 834 1075
Fragments arranged by size:
1226 1075 834
BssSI C'ACGA_G
Cuts at: 0 115 223 2364 3135
Size: 115 108 2141 771
Fragments arranged by size:
2141 771 115 108
BstBI TT'CG_AA
Cuts at: 0 3050 3135
Size: 3050 85
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2505 2568 3135
Size: 2505 63 567
Fragments arranged by size:
2505 567 63
BstYI r'GATC_y
Cuts at: 0 285 1671 3135
Size: 285 1386 1464
Fragments arranged by size:
1464 1386 285
BtgI C'CryG_G
Cuts at: 0 1301 1340 2181 2403 2648 2718 2768 3135
Size: 1301 39 841 222 245 70 50 367
Fragments arranged by size:
1301 841 367 245 222 70 50 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1886 2678 2769 3135
Size: 1886 792 91 366
Fragments arranged by size:
1886 792 366 91
BtsI GCAGTG_nn'
Cuts at: 0 2053 2466 2795 3081 3135
Size: 2053 413 329 286 54
Fragments arranged by size:
2053 413 329 286 54
DraI TTT'AAA
Cuts at: 0 2397 3135
Size: 2397 738
DraIII CAC_nnn'GTG
Cuts at: 0 278 489 1691 3135
Size: 278 211 1202 1444
Fragments arranged by size:
1444 1202 278 211
EaeI y'GGCC_r
Cuts at: 0 692 725 841 887 2050 2507 2558 2606
Size: 692 33 116 46 1163 457 51 48
Cuts at: 2606 3135
Size: 529
Fragments arranged by size:
1163 692 529 457 116 51 48 46
33
EarI CTCTTCn'nnn_
Cuts at: 0 953 1343 1594 2572 3135
Size: 953 390 251 978 563
Fragments arranged by size:
978 953 563 390 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 350 1242 1333 2587 3046 3135
Size: 350 892 91 1254 459 89
Fragments arranged by size:
1254 892 459 350 91 89
EcoICRI GAG'CTC
Cuts at: 0 178 1970 3135
Size: 178 1792 1165
Fragments arranged by size:
1792 1165 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1177 1193 1687 1903 1972 2002 2107
Size: 89 1088 16 494 216 69 30 105
Cuts at: 2107 2112 2305 2719 2763 3009 3135
Size: 5 193 414 44 246 126
Fragments arranged by size:
1088 494 414 246 216 193 126 105
89 69 44 30 16 5
EcoO109I rG'GnC_Cy
Cuts at: 0 44 45 1373 1947 2082 2709 3135
Size: 44 1 1328 574 135 627 426
Fragments arranged by size:
1328 627 574 426 135 44 1
EcoRI G'AATT_C
Cuts at: 0 743 877 1143 3135
Size: 743 134 266 1992
Fragments arranged by size:
1992 743 266 134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 444 825 3018 3135
Size: 444 381 2193 117
Fragments arranged by size:
2193 444 381 117
HaeII r_GCGC'y
Cuts at: 0 825 1339 2684 2706 3135
Size: 825 514 1345 22 429
Fragments arranged by size:
1345 825 514 429 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 642 674 876 908 1252 1284 1413 1445
Size: 642 32 202 32 344 32 129 32
Cuts at: 1445 1479 1511 2614 2646 2647 2679 3135
Size: 34 32 1103 32 1 32 456
Fragments arranged by size:
1103 642 456 344 202 129 34 32
32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 0 446 3135
Size: 446 2689
KasI G'GCGC_C
Cuts at: 0 1335 3135
Size: 1335 1800
KpnI G_GTAC'C
Cuts at: 0 402 3135
Size: 402 2733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 3135
Size: 1003 2132
MscI TGG'CCA
Cuts at: 0 889 2560 2608 3135
Size: 889 1671 48 527
Fragments arranged by size:
1671 889 527 48
MslI CAynn'nnrTG
Cuts at: 0 2164 2503 2554 2731 2788 3135
Size: 2164 339 51 177 57 347
Fragments arranged by size:
2164 347 339 177 57 51
MspA1I CmG'CkG
Cuts at: 0 1303 1545 2490 2650 2720 3135
Size: 1303 242 945 160 70 415
Fragments arranged by size:
1303 945 415 242 160 70
NaeI GCC'GGC
Cuts at: 0 1228 3135
Size: 1228 1907
NarI GG'CG_CC
Cuts at: 0 1336 3135
Size: 1336 1799
NcoI C'CATG_G
Cuts at: 0 2768 3135
Size: 2768 367
NgoMIV G'CCGG_C
Cuts at: 0 1226 3135
Size: 1226 1909
NruI TCG'CGA
Cuts at: 0 3096 3135
Size: 3096 39
NspI r_CATG'y
Cuts at: 0 2334 3135
Size: 2334 801
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 2085 3135
Size: 138 1947 1050
Fragments arranged by size:
1947 1050 138
PfoI T'CCnGG_A
Cuts at: 0 2084 2265 3135
Size: 2084 181 870
Fragments arranged by size:
2084 870 181
PmlI CAC'GTG
Cuts at: 0 2739 3105 3135
Size: 2739 366 30
Fragments arranged by size:
2739 366 30
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3031 3063 3135
Size: 3031 32 72
Fragments arranged by size:
3031 72 32
PpuMI rG'GwC_Cy
Cuts at: 0 1373 2082 3135
Size: 1373 709 1053
Fragments arranged by size:
1373 1053 709
PspOMI G'GGCC_C
Cuts at: 0 44 3135
Size: 44 3091
PspXI vC'TCGA_Gb
Cuts at: 0 247 3135
Size: 247 2888
PstI C_TGCA'G
Cuts at: 0 416 1996 2012 3135
Size: 416 1580 16 1123
Fragments arranged by size:
1580 1123 416 16
RsrII CG'GwC_CG
Cuts at: 0 2522 3135
Size: 2522 613
SacI G_AGCT'C
Cuts at: 0 180 1972 3135
Size: 180 1792 1163
Fragments arranged by size:
1792 1163 180
SacII CC_GC'GG
Cuts at: 0 1304 2651 2721 3135
Size: 1304 1347 70 414
Fragments arranged by size:
1347 1304 414 70
SapI GCTCTTCn'nnn_
Cuts at: 0 953 2572 3135
Size: 953 1619 563
Fragments arranged by size:
1619 953 563
SexAI A'CCwGG_T
Cuts at: 0 2078 3135
Size: 2078 1057
SfcI C'TryA_G
Cuts at: 0 412 1133 1992 2008 3135
Size: 412 721 859 16 1127
Fragments arranged by size:
1127 859 721 412 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1945 3135
Size: 1945 1190
SfoI GGC'GCC
Cuts at: 0 1337 3135
Size: 1337 1798
SmlI C'TyrA_G
Cuts at: 0 247 434 1795 2227 3135
Size: 247 187 1361 432 908
Fragments arranged by size:
1361 908 432 247 187
StuI AGG'CCT
Cuts at: 0 1525 1561 1939 3135
Size: 1525 36 378 1196
Fragments arranged by size:
1525 1196 378 36
StyI C'CwwG_G
Cuts at: 0 71 521 695 791 872 1940 2768 3135
Size: 71 450 174 96 81 1068 828 367
Fragments arranged by size:
1068 828 450 367 174 96 81 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 2510 2847 3135
Size: 163 2347 337 288
Fragments arranged by size:
2347 337 288 163
TatI w'GTAC_w
Cuts at: 0 3041 3135
Size: 3041 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 101 133 734 766 909 941 2080 2112
Size: 101 32 601 32 143 32 1139 32
Cuts at: 2112 2992 3024 3135
Size: 880 32 111
Fragments arranged by size:
1139 880 601 143 111 101 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1491 3135
Size: 1491 1644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 607 2766 3135
Size: 607 2159 369
Fragments arranged by size:
2159 607 369
XhoI C'TCGA_G
Cuts at: 0 247 3135
Size: 247 2888
XmnI GAAnn'nnTTC
Cuts at: 0 1603 3135
Size: 1603 1532
ZraI GAC'GTC
Cuts at: 0 2352 3135
Size: 2352 783
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PstI RsrII SacI SacII
SapI SexAI SfcI SfiI SfoI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BfrBI BglII BmtI BsaI BsaBI
BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstAPI
BstEII BstZ17I Bsu36I ClaI CspCI DrdI EagI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI
NdeI NheI NotI NsiI PacI PasI PciI PmeI
PshAI PsiI PsrI PvuI PvuII SalI SanDI SbfI
ScaI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XmaI