(Circular) (Six-base) MAPSORT of: pFN21AB5603.seq Check: 7616 from: 1 to: 6103 pFN21AB5603 6103 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 1937 Size: 1509 4594 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2541 4166 5550 5818 1632 Size: 462 447 1625 1384 268 1917 Fragments arranged by size: 1917 1625 1384 462 447 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 2359 2791 5002 5979 1203 Size: 1156 432 2211 977 1327 Fragments arranged by size: 2211 1327 1156 977 432 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 3846 5418 3846 Size: 1572 4531 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 2165 2864 3005 3412 3638 3790 5899 1493 Size: 672 699 141 407 226 152 2109 1697 Fragments arranged by size: 2109 1697 699 672 407 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2298 3428 1870 Size: 73 355 1130 4545 Fragments arranged by size: 4545 1130 355 73 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3117 3424 4879 618 Size: 325 217 627 1330 307 1455 1842 Fragments arranged by size: 1842 1455 1330 627 325 307 217 BanII G_rGCy'C Cuts at: 1391 3422 1391 Size: 2031 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2145 2440 2760 928 Size: 971 246 295 320 4271 Fragments arranged by size: 4271 971 320 295 246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3190 6098 3190 Size: 2908 3195 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 4164 5320 1790 Size: 2374 1156 2573 Fragments arranged by size: 2573 2374 1156 BmgBI CAC'GTC Cuts at: 1318 2256 1318 Size: 938 5165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2767 3834 4792 Size: 416 53 83 399 24 1350 1067 958 Cuts at: 4792 442 Size: 1753 Fragments arranged by size: 1753 1350 1067 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2484 2691 3341 4763 1239 Size: 1245 207 650 1422 2579 Fragments arranged by size: 2579 1422 1245 650 207 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4228 5093 5391 4228 Size: 865 298 4940 Fragments arranged by size: 4940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2620 4766 882 Size: 617 1121 2146 2219 Fragments arranged by size: 2219 2146 1121 617 BsaAI yAC'GTr Cuts at: 493 1204 3894 493 Size: 711 2690 2702 Fragments arranged by size: 2702 2690 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3910 4292 4927 275 Size: 53 83 186 936 2377 382 635 1451 Fragments arranged by size: 2377 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2860 4536 4934 5208 5355 5766 2860 Size: 1676 398 274 147 411 3197 Fragments arranged by size: 3197 1676 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5878 Fragments arranged by size: 5878 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 1186 Size: 944 5159 BseYI C'CCAG_C Cuts at: 5306 5802 5306 Size: 496 5607 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4164 4249 5320 4164 Size: 85 1071 4947 Fragments arranged by size: 4947 1071 85 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3422 4164 4249 5320 1391 Size: 399 1632 742 85 1071 2174 Fragments arranged by size: 2174 1632 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 3992 5722 3992 Size: 1730 4373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2839 3458 844 Size: 1995 619 3489 Fragments arranged by size: 3489 1995 619 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 3120 4747 4934 1700 Size: 110 331 979 1627 187 2869 Fragments arranged by size: 2869 1627 979 331 187 110 BsrGI T'GTAC_A Cuts at: 96 3148 96 Size: 3052 3051 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 3388 794 Size: 2594 3509 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstYI r'GATC_y Cuts at: 1972 3190 3433 3800 4185 4202 5654 5740 5752 Size: 1218 243 367 385 17 1452 86 12 Cuts at: 5752 6098 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1218 385 367 346 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2187 2187 Size: 6103 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5551 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2061 3619 4413 4433 2061 Size: 1558 794 20 3731 Fragments arranged by size: 3731 1558 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2313 5110 809 Size: 507 997 2797 1802 Fragments arranged by size: 2797 1802 997 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3550 4439 6068 Size: 54 1425 50 275 18 1720 889 1629 Cuts at: 6068 8 Size: 43 Fragments arranged by size: 1720 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 2841 3181 4033 1353 Size: 834 654 340 852 3423 Fragments arranged by size: 3423 852 834 654 340 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2484 2541 2691 3341 4166 4763 Size: 393 462 390 57 150 650 825 597 Cuts at: 4763 5550 5818 1239 Size: 787 268 1524 Fragments arranged by size: 1524 825 787 650 597 462 393 390 268 150 57 EcoNI CCTnn'n_nnAGG Cuts at: 2112 2112 Size: 6103 EcoRI G'AATT_C Cuts at: 2864 3005 3412 3790 2864 Size: 141 407 378 5177 Fragments arranged by size: 5177 407 378 141 EcoRV GAT'ATC Cuts at: 2295 2680 2295 Size: 385 5718 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2436 2436 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 5250 1678 Size: 3572 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 3345 3377 4744 Size: 32 135 32 616 32 1125 32 1367 Cuts at: 4744 4776 4818 4850 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1367 1125 616 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2259 3447 3693 669 Size: 988 281 321 1188 246 3079 Fragments arranged by size: 3079 1188 988 321 281 246 HindIII A'AGCT_T Cuts at: 748 2025 3326 748 Size: 1277 1301 3525 Fragments arranged by size: 3525 1301 1277 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5217 5401 1305 Size: 3912 184 2007 Fragments arranged by size: 3912 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4281 Fragments arranged by size: 4281 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4061 4420 4579 518 Size: 648 2895 359 159 2042 Fragments arranged by size: 2895 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3519 4196 5344 5589 1606 Size: 1913 677 1148 245 2120 Fragments arranged by size: 2120 1913 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5551 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2363 2795 3461 5006 1132 Size: 97 1134 432 666 1545 2229 Fragments arranged by size: 2229 1545 1134 666 432 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 2359 2791 5002 2359 Size: 432 2211 3460 Fragments arranged by size: 3460 2211 432 PflMI CCAn_nnn'nTGG Cuts at: 1108 2714 1108 Size: 1606 4497 PfoI T'CCnGG_A Cuts at: 2500 3009 3318 2500 Size: 509 309 5285 Fragments arranged by size: 5285 509 309 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PstI C_TGCA'G Cuts at: 830 3455 830 Size: 2625 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SapI GCTCTTCn'nnn_ Cuts at: 2841 2841 Size: 6103 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 2575 4351 6063 2575 Size: 1776 1712 2615 Fragments arranged by size: 2615 1776 1712 SfcI C'TryA_G Cuts at: 826 1045 3261 3451 4586 5267 5458 826 Size: 219 2216 190 1135 681 191 1471 Fragments arranged by size: 2216 1471 1135 681 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3430 1872 Size: 1558 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2298 4207 5108 5370 820 Size: 197 926 355 1909 901 262 1553 Fragments arranged by size: 1909 1553 926 901 355 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 2896 152 Size: 2744 3359 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 2084 3178 4027 5 Size: 47 2032 1094 849 2081 Fragments arranged by size: 2081 2032 1094 849 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6103 StyI C'CwwG_G Cuts at: 513 1065 2411 3542 513 Size: 552 1346 1131 3074 Fragments arranged by size: 3074 1346 1131 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4130 4147 4300 4485 4930 325 Size: 1255 2550 17 153 185 445 1498 Fragments arranged by size: 2550 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2573 3148 4349 Size: 274 80 33 51 1443 596 575 1201 Cuts at: 4349 6061 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1201 596 575 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 6063 6095 2496 Size: 32 3535 32 2504 Fragments arranged by size: 3535 2504 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 2298 1943 Size: 355 5748 XmaI C'CCGG_G Cuts at: 1870 3428 1870 Size: 1558 4545 XmnI GAAnn'nnTTC Cuts at: 3348 4232 3348 Size: 884 5219 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI