Restriction map for
pFN21AB5520
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MboI
MfeI MflI MluI MroI MspA1I
MunI NarI NcoI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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ApaI 868 GGGCCC GGGCC^C
AspI 408 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 663 TGGCCA TGG^CCA
BanII 868 GGGCCC G[GA]GC[CT]^C
BbvCI 715 GCTGAGG CCTCAGC(-5/-2)
BcgI 699 CGACGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 783 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 97 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 28 GTGCAG GTGCAG(16/14)
BspDI 567 ATCGAT AT^CGAT
BspMI 895 GCAGGT ACCTGC(4/8)
BsrFI 763 GCCGGC [GA]^CCGG[CT]
Cfr10I 763 GCCGGC [GA]^CCGG[CT]
ClaI 567 ATCGAT AT^CGAT
DsaI 917 CCGTGG C^C[GA][CT]GG
EarI 525 CTCTTC CTCTTC(1/4)
EcoNI 711 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 570 GATATC GAT^ATC
FauI 774 CCCGC CCCGC(4/6)
HgaI 886 GCGTC GACGC(5/10)
HinfI 372 GAATC G^A[ACGT]TC
HphI 43 TCACC GGTGA(8/7)
Ksp632I 525 CTCTTC CTCTTC(1/4)
MaeII 532 ACGT A^CGT
MamI 97 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 663 TGGCCA TGG^CCA
MscI 663 TGGCCA TGG^CCA
MslI 80 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 763 GCCGGC GCC^GGC
NdeI 155 CATATG CA^TATG
NgoMIV 763 GCCGGC G^CCGGC
PflFI 408 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SmaI 865 CCCGGG CCC^GGG
SphI 725 GCATGC GCATG^C
TfiI 372 GAATC G^A[AT]TC
Tth111I 408 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 865 CCCGGG C^CCGGG
XmaI 865 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanI 861 GGCACC G^G[CT][GA]CC
949 GGTGCC
BbsI 346 GAAGAC GAAGAC(2/6)
295 GTCTTC
BfaI 22 CTAG C^TAG
499 CTAG
BmyI 860 GGGCAC G[AGT]GC[ACT]^C
868 GGGCCC
BpuAI 346 GAAGAC GAAGAC(2/6)
295 GTCTTC
Bsp1286I 860 GGGCAC G[AGT]GC[ACT]^C
868 GGGCCC
BsrI 75 ACTGG ACTGG(1/-1)
666 CCAGT
BstF5I 91 CATCC GGATG(2/0)
171 GGATG
BstNI 872 CCTGG CC^[AT]GG
981 CCTGG
EaeI 663 TGGCCA [CT]^GGCC[GA]
695 TGGCCG
EcoRII 872 CCTGG ^CC[AT]GG
981 CCTGG
FokI 91 CATCC GGATG(9/13)
171 GGATG
MaeI 22 CTAG C^TAG
499 CTAG
MvaI 872 CCTGG CC^[AT]GG
981 CCTGG
NspI 36 ACATGC [GA]CATG^[CT]
725 GCATGC
Tsp45I 495 GTCAC ^GT[GC]AC
503 GTCAC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AccII AciI AcsI ApoI AvaI
AvaII BbvI BcnI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
BssKI BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MboII MnlI MseI MspI
MvnI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I