(Circular) (Six-base) MAPSORT of: pFN21AB5514.seq Check: 4731 from: 1 to: 6046 pFN21AB5514 6046 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2342 2733 3389 1937 Size: 405 391 656 4594 Fragments arranged by size: 4594 656 405 391 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2841 3085 3200 4109 5493 5761 1632 Size: 1209 244 115 909 1384 268 1917 Fragments arranged by size: 1917 1384 1209 909 268 244 115 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 2920 4775 2920 Size: 1855 4191 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2015 3789 5361 2015 Size: 1774 1572 2700 Fragments arranged by size: 2700 1774 1572 ApaLI G'TGCA_C Cuts at: 4103 5259 4103 Size: 1156 4890 ApoI r'AATT_y Cuts at: 1493 2108 2702 2879 2931 3038 3355 3581 3733 Size: 615 594 177 52 107 317 226 152 Cuts at: 3733 5842 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 615 594 317 226 177 152 107 52 AseI AT'TA_AT Cuts at: 160 1032 2559 2604 4600 160 Size: 872 1527 45 1996 1606 Fragments arranged by size: 1996 1606 1527 872 45 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 3371 1870 Size: 73 1428 4545 Fragments arranged by size: 4545 1428 73 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3276 3367 4822 618 Size: 325 217 627 1489 91 1455 1842 Fragments arranged by size: 1842 1489 1455 627 325 217 91 BanII G_rGCy'C Cuts at: 1391 3365 1391 Size: 1974 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5075 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 4107 5263 1790 Size: 2317 1156 2573 Fragments arranged by size: 2573 2317 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2823 3777 4735 Size: 416 53 83 399 24 1406 954 958 Cuts at: 4735 442 Size: 1753 Fragments arranged by size: 1753 1406 958 954 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2142 4706 1239 Size: 798 105 2564 2579 Fragments arranged by size: 2579 2564 798 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4709 882 Size: 617 3210 2219 Fragments arranged by size: 3210 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3853 4235 4870 275 Size: 53 83 186 936 2320 382 635 1451 Fragments arranged by size: 2320 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4479 4877 5151 5298 5709 4479 Size: 398 274 147 411 4816 Fragments arranged by size: 4816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5821 Fragments arranged by size: 5821 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2034 2223 2432 2851 1186 Size: 848 189 209 419 4381 Fragments arranged by size: 4381 848 419 209 189 BseYI C'CCAG_C Cuts at: 5249 5745 5249 Size: 496 5550 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4107 4192 5263 4107 Size: 85 1071 4890 Fragments arranged by size: 4890 1071 85 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3365 4107 4192 5263 1391 Size: 399 1575 742 85 1071 2174 Fragments arranged by size: 2174 1575 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3075 3935 5665 3075 Size: 860 1730 3456 Fragments arranged by size: 3456 1730 860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3401 844 Size: 2557 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3294 4541 4715 65 Size: 1183 2046 1247 174 1396 Fragments arranged by size: 2046 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4690 4877 1700 Size: 110 2880 187 2869 Fragments arranged by size: 2880 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6046 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 2307 1184 Size: 1123 4923 BstYI r'GATC_y Cuts at: 1972 2437 2485 2824 3059 3083 3376 3743 4128 Size: 465 48 339 235 24 293 367 385 Cuts at: 4128 4145 5597 5683 5695 6041 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 465 385 367 346 339 293 235 86 48 24 17 12 BstZ17I GTA'TAC Cuts at: 2734 2734 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3132 487 Size: 683 56 1906 3401 Fragments arranged by size: 3401 1906 683 56 BtsI GCAGTG_nn' Cuts at: 3562 4356 4376 3562 Size: 794 20 5232 Fragments arranged by size: 5232 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3071 5053 809 Size: 507 1755 1982 1802 Fragments arranged by size: 1982 1802 1755 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3493 4382 6011 Size: 54 1425 50 275 18 1663 889 1629 Cuts at: 6011 8 Size: 43 Fragments arranged by size: 1663 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2667 3976 1353 Size: 1314 1309 3423 Fragments arranged by size: 3423 1314 1309 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2142 2841 3085 3200 4109 4706 Size: 393 405 105 699 244 115 909 597 Cuts at: 4706 5493 5761 1239 Size: 787 268 1524 Fragments arranged by size: 1524 909 787 699 597 405 393 268 244 115 105 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 EcoRV GAT'ATC Cuts at: 2653 2653 Size: 6046 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 5193 1678 Size: 3515 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2454 2486 2906 Size: 32 135 32 576 32 274 32 420 Cuts at: 2906 2938 4687 4719 4761 4793 1373 Size: 32 1749 32 42 32 2626 Fragments arranged by size: 2626 1749 576 420 274 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3235 3390 3636 669 Size: 988 281 1297 155 246 3079 Fragments arranged by size: 3079 1297 988 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3032 5160 5344 1305 Size: 1727 2128 184 2007 Fragments arranged by size: 2128 2007 1727 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4224 Fragments arranged by size: 4224 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4004 4363 4522 518 Size: 648 2838 359 159 2042 Fragments arranged by size: 2838 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2718 3462 4139 5287 5532 1606 Size: 1112 744 677 1148 245 2120 Fragments arranged by size: 2120 1148 1112 744 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3404 4949 1132 Size: 97 2175 1545 2229 Fragments arranged by size: 2229 2175 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6046 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2989 3021 2989 Size: 32 6014 PstI C_TGCA'G Cuts at: 830 2590 3398 830 Size: 1760 808 3478 Fragments arranged by size: 3478 1760 808 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 2718 2718 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SapI GCTCTTCn'nnn_ Cuts at: 2667 2667 Size: 6046 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SexAI A'CCwGG_T Cuts at: 2973 2973 Size: 6046 SfcI C'TryA_G Cuts at: 826 1045 2100 2445 2586 3394 4529 5210 5401 Size: 219 1055 345 141 808 1135 681 191 Cuts at: 5401 826 Size: 1471 Fragments arranged by size: 1471 1135 1055 808 681 345 219 191 141 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 3373 1872 Size: 1501 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 2517 3970 5 Size: 47 2465 1453 2081 Fragments arranged by size: 2465 2081 1453 47 StuI AGG'CCT Cuts at: 1517 2479 2959 1517 Size: 962 480 4604 Fragments arranged by size: 4604 962 480 StyI C'CwwG_G Cuts at: 513 1065 2004 3282 3485 513 Size: 552 939 1278 203 3074 Fragments arranged by size: 3074 1278 939 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4292 6004 96 Size: 274 80 33 51 1443 2315 1712 138 Fragments arranged by size: 2315 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2362 2394 6006 6038 2362 Size: 32 3612 32 2370 Fragments arranged by size: 3612 2370 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 3371 1870 Size: 1501 4545 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI