(Circular) (Six-base) MAPSORT of: pFN21AB5513.seq Check: 9242 from: 1 to: 6805 pFN21AB5513 6805 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4615 278 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4148 1937 Size: 2211 4594 Acc65I G'GTAC_C Cuts at: 3863 4126 3863 Size: 263 6542 AclI AA'CG_TT Cuts at: 1563 4932 5305 1563 Size: 3369 373 3063 Fragments arranged by size: 3369 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3703 3806 4868 6252 6520 1632 Size: 2071 103 1062 1384 268 1917 Fragments arranged by size: 2071 1917 1384 1062 268 103 AfeI AGC'GCT Cuts at: 3386 3386 Size: 6805 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6608 AflIII A'CryG_T Cuts at: 1203 2790 5704 6681 1203 Size: 1587 2914 977 1327 Fragments arranged by size: 2914 1587 1327 977 AgeI A'CCGG_T Cuts at: 5636 5636 Size: 6805 AhdI GACnn_n'nnGTC Cuts at: 5534 5534 Size: 6805 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6805 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6805 AlwNI CAG_nnn'CTG Cuts at: 2776 2845 3166 4548 6120 2776 Size: 69 321 1382 1572 3461 Fragments arranged by size: 3461 1572 1382 321 69 ApaI G_GGCC'C Cuts at: 2199 2199 Size: 6805 ApaLI G'TGCA_C Cuts at: 4862 6018 4862 Size: 1156 5649 ApoI r'AATT_y Cuts at: 1493 2972 3831 3871 4114 4340 4492 6601 1493 Size: 1479 859 40 243 226 152 2109 1697 Fragments arranged by size: 2109 1697 1479 859 243 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 5359 160 Size: 872 4327 1606 Fragments arranged by size: 4327 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2370 4046 4130 1870 Size: 73 271 156 1676 84 4545 Fragments arranged by size: 4545 1676 271 156 84 73 AvrII C'CTAG_G Cuts at: 2308 2308 Size: 6805 BamHI G'GATC_C Cuts at: 4042 4135 4502 4042 Size: 93 367 6345 Fragments arranged by size: 6345 367 93 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2597 3863 4126 5581 618 Size: 325 217 627 810 1266 263 1455 1842 Fragments arranged by size: 1842 1455 1266 810 627 325 263 217 BanII G_rGCy'C Cuts at: 1391 2063 2199 2308 3110 3237 4071 4124 1391 Size: 672 136 109 802 127 834 53 4072 Fragments arranged by size: 4072 834 802 672 136 127 109 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2625 928 Size: 971 726 5108 Fragments arranged by size: 5108 971 726 BbvCI CC'TCA_GC Cuts at: 3347 3347 Size: 6805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4996 5030 1903 Size: 34 3059 34 3678 Fragments arranged by size: 3678 3059 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4697 5907 4697 Size: 1210 5595 BclI T'GATC_A Cuts at: 1548 3168 1548 Size: 1620 5185 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 3626 5416 Size: 107 122 71 631 605 355 1599 1790 Cuts at: 5416 136 Size: 1525 Fragments arranged by size: 1790 1599 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 2244 3021 3645 6800 2244 Size: 777 624 3155 2249 Fragments arranged by size: 3155 2249 777 624 BlpI GC'TnA_GC Cuts at: 4222 4222 Size: 6805 Bme1580I G_kGCm'C Cuts at: 1790 2199 2790 4866 6022 1790 Size: 409 591 2076 1156 2573 Fragments arranged by size: 2573 2076 1156 591 409 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6805 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 3616 4536 Size: 416 53 83 399 24 837 1362 920 Cuts at: 4536 5494 442 Size: 958 1753 Fragments arranged by size: 1753 1362 958 920 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6805 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2188 2554 2567 3038 3118 3197 5465 Size: 905 44 366 13 471 80 79 2268 Cuts at: 5465 1239 Size: 2579 Fragments arranged by size: 2579 2268 905 471 366 80 79 44 13 Bpu10I CC'TnA_GC Cuts at: 3347 3347 Size: 6805 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4930 5795 6093 4930 Size: 865 298 5642 Fragments arranged by size: 5642 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5468 882 Size: 617 3969 2219 Fragments arranged by size: 3969 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4596 493 Size: 711 3392 2702 Fragments arranged by size: 3392 2702 711 BsaBI GATnn'nnATC Cuts at: 2852 6799 2852 Size: 3947 2858 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4612 4994 5629 275 Size: 53 83 186 936 3079 382 635 1451 Fragments arranged by size: 3079 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2627 2804 5238 5636 5910 6057 6468 2627 Size: 177 2434 398 274 147 411 2964 Fragments arranged by size: 2964 2434 411 398 274 177 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6580 Fragments arranged by size: 6580 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2289 2828 2948 3194 3197 3212 3332 1186 Size: 1103 539 120 246 3 15 120 4659 Fragments arranged by size: 4659 1103 539 246 120 120 15 3 BseYI C'CCAG_C Cuts at: 2056 3778 6008 6504 2056 Size: 1722 2230 496 2357 Fragments arranged by size: 2357 2230 1722 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3202 1163 Size: 573 1466 4766 Fragments arranged by size: 4766 1466 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 4255 5016 6044 1540 Size: 275 186 48 2206 761 1028 2301 Fragments arranged by size: 2301 2206 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 3420 4071 4866 4951 6022 3420 Size: 651 795 85 1071 4203 Fragments arranged by size: 4203 1071 795 651 85 BsmI GAATG_Cn' Cuts at: 2509 4316 4409 2509 Size: 1807 93 4905 Fragments arranged by size: 4905 1807 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2199 2308 2790 3110 3237 3420 Size: 399 273 136 109 482 320 127 183 Cuts at: 3420 4071 4124 4866 4951 6022 1391 Size: 651 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 482 399 320 273 183 136 127 109 85 53 BspEI T'CCGG_A Cuts at: 6468 6468 Size: 6805 BspHI T'CATG_A Cuts at: 4694 6424 4694 Size: 1730 5075 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3896 4160 844 Size: 3052 264 3489 Fragments arranged by size: 3489 3052 264 BsrBI CCG'CTC Cuts at: 1394 1823 3213 4692 1394 Size: 429 1390 1479 3507 Fragments arranged by size: 3507 1479 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 2634 5300 5474 65 Size: 1183 1086 300 2666 174 1396 Fragments arranged by size: 2666 1396 1183 1086 300 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5449 5636 1700 Size: 110 3639 187 2869 Fragments arranged by size: 3639 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6805 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6805 BssSI C'ACGA_G Cuts at: 2066 3455 4865 5877 2066 Size: 1389 1410 1012 2994 Fragments arranged by size: 2994 1410 1389 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2336 794 Size: 1542 5263 BstBI TT'CG_AA Cuts at: 2616 3471 5609 2616 Size: 855 2138 3812 Fragments arranged by size: 3812 2138 855 BstEII G'GTnAC_C Cuts at: 1184 2800 1184 Size: 1616 5189 BstYI r'GATC_y Cuts at: 1972 2244 2415 3021 3645 4042 4135 4502 4887 Size: 272 171 606 624 397 93 367 385 Cuts at: 4887 4904 6356 6442 6454 6800 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 624 606 397 385 367 346 272 171 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2999 2999 Size: 6805 BtgI C'CryG_G Cuts at: 513 1065 2004 2238 2745 3114 513 Size: 552 939 234 507 369 4204 Fragments arranged by size: 4204 939 552 507 369 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 1999 487 Size: 683 56 773 5293 Fragments arranged by size: 5293 773 683 56 BtsI GCAGTG_nn' Cuts at: 4321 5115 5135 4321 Size: 794 20 5991 Fragments arranged by size: 5991 794 20 ClaI AT'CG_AT Cuts at: 1554 3975 1554 Size: 2421 4384 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6770 DraI TTT'AAA Cuts at: 4109 4109 Size: 6805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5812 809 Size: 507 4496 1802 Fragments arranged by size: 4496 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2046 2160 Size: 54 1425 50 275 18 189 27 114 Cuts at: 2160 4252 5141 6770 8 Size: 2092 889 1629 43 Fragments arranged by size: 2092 1629 1425 889 275 189 114 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 4252 1537 Size: 275 234 2206 4090 Fragments arranged by size: 4090 2206 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 3561 4735 1353 Size: 791 1417 1174 3423 Fragments arranged by size: 3423 1417 1174 791 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5392 5766 5912 387 Size: 1064 138 3803 374 146 1280 Fragments arranged by size: 3803 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4069 4069 Size: 6805 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2188 2554 2567 3038 3118 3197 Size: 393 512 44 366 13 471 80 79 Cuts at: 3197 3703 3806 4868 5465 6252 6520 1239 Size: 506 103 1062 597 787 268 1524 Fragments arranged by size: 1524 1062 787 597 512 506 471 393 366 268 103 80 79 44 13 EcoNI CCTnn'n_nnAGG Cuts at: 2090 3410 2090 Size: 1320 5485 EcoO109I rG'GnC_Cy Cuts at: 2146 2195 2196 2146 Size: 49 1 6755 Fragments arranged by size: 6755 49 1 EcoRI G'AATT_C Cuts at: 2972 3871 4114 4492 2972 Size: 899 243 378 5285 Fragments arranged by size: 5285 899 378 243 EcoRV GAT'ATC Cuts at: 3703 3703 Size: 6805 FspI TGC'GCA Cuts at: 5311 5311 Size: 6805 HaeII r_GCGC'y Cuts at: 1678 2108 2236 2541 2745 3388 3697 5952 1678 Size: 430 128 305 204 643 309 2255 2531 Fragments arranged by size: 2531 2255 643 430 309 305 204 128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3142 3174 3692 3724 3907 Size: 32 135 32 1570 32 518 32 183 Cuts at: 3907 3939 4064 4096 5446 5478 5520 5552 1373 Size: 32 125 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1570 1350 518 183 135 125 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3748 3853 3926 4149 4395 669 Size: 988 281 1810 105 73 223 246 3079 Fragments arranged by size: 3079 1810 988 281 246 223 105 73 HindIII A'AGCT_T Cuts at: 748 748 Size: 6805 HpaI GTT'AAC Cuts at: 4395 4395 Size: 6805 KpnI G_GTAC'C Cuts at: 3867 4130 3867 Size: 263 6542 MfeI C'AATT_G Cuts at: 4404 4404 Size: 6805 MluI A'CGCG_T Cuts at: 2790 6681 2790 Size: 3891 2914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5919 6103 1305 Size: 4614 184 2007 Fragments arranged by size: 4614 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4983 Fragments arranged by size: 4983 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2964 3083 3455 4763 5122 5281 518 Size: 648 1798 119 372 1308 359 159 2042 Fragments arranged by size: 2042 1798 1308 648 372 359 159 119 MspA1I CmG'CkG Cuts at: 1606 2747 3116 4039 4221 4898 6046 6291 1606 Size: 1141 369 923 182 677 1148 245 2120 Fragments arranged by size: 2120 1148 1141 923 677 369 245 182 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5314 Fragments arranged by size: 5314 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6805 NotI GC'GGCC_GC Cuts at: 4252 4252 Size: 6805 NspI r_CATG'y Cuts at: 1132 1229 3283 3735 3754 4163 5708 1132 Size: 97 2054 452 19 409 1545 2229 Fragments arranged by size: 2229 2054 1545 452 409 97 19 PasI CC'CwG_GG Cuts at: 1348 2176 2865 1348 Size: 828 689 5288 Fragments arranged by size: 5288 828 689 PciI A'CATG_T Cuts at: 5704 5704 Size: 6805 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6805 PfoI T'CCnGG_A Cuts at: 2142 2397 3408 2142 Size: 255 1011 5539 Fragments arranged by size: 5539 1011 255 PmeI GTTT'AAAC Cuts at: 4109 4109 Size: 6805 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4874 4906 6387 6419 4874 Size: 32 1481 32 5260 Fragments arranged by size: 5260 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 2146 Size: 6805 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6805 PsiI TTA'TAA Cuts at: 4375 4375 Size: 6805 PspOMI G'GGCC_C Cuts at: 2195 2195 Size: 6805 PspXI vC'TCGA_Gb Cuts at: 2370 2370 Size: 6805 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3146 3178 3146 Size: 32 6773 PstI C_TGCA'G Cuts at: 830 3666 4157 830 Size: 2836 491 3478 Fragments arranged by size: 3478 2836 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6805 PvuII CAG'CTG Cuts at: 4039 4039 Size: 6805 SacI G_AGCT'C Cuts at: 4071 4071 Size: 6805 SacII CC_GC'GG Cuts at: 2748 3117 2748 Size: 369 6436 SalI G'TCGA_C Cuts at: 1936 4147 1936 Size: 2211 4594 SapI GCTCTTCn'nnn_ Cuts at: 2144 2144 Size: 6805 SbfI CC_TGCA'GG Cuts at: 4157 4157 Size: 6805 ScaI AGT'ACT Cuts at: 4101 5053 6765 4101 Size: 952 1712 4141 Fragments arranged by size: 4141 1712 952 SfcI C'TryA_G Cuts at: 826 1045 2837 2922 3662 4153 5288 5969 6160 Size: 219 1792 85 740 491 1135 681 191 Cuts at: 6160 826 Size: 1471 Fragments arranged by size: 1792 1471 1135 740 681 491 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6805 SmaI CCC'GGG Cuts at: 1872 2216 4132 1872 Size: 344 1916 4545 Fragments arranged by size: 4545 1916 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 2370 4909 5810 6072 820 Size: 197 926 427 2539 901 262 1553 Fragments arranged by size: 2539 1553 926 901 427 262 197 SnaBI TAC'GTA Cuts at: 493 4596 493 Size: 4103 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6805 SphI G_CATG'C Cuts at: 1132 4163 1132 Size: 3031 3774 SspI AAT'ATT Cuts at: 5 52 4729 5 Size: 47 4677 2081 Fragments arranged by size: 4677 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6805 StyI C'CwwG_G Cuts at: 513 1065 2004 2299 2308 2484 2716 4244 513 Size: 552 939 295 9 176 232 1528 3074 Fragments arranged by size: 3074 1528 939 552 295 232 176 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 2788 4832 4849 5002 5187 5632 Size: 1255 860 348 2044 17 153 185 445 Cuts at: 5632 325 Size: 1498 Fragments arranged by size: 2044 1498 1255 860 445 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3447 4099 5051 Size: 274 80 33 51 1443 1470 652 952 Cuts at: 5051 6763 96 Size: 1712 138 Fragments arranged by size: 1712 1470 1443 952 652 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6765 6797 6765 Size: 32 6773 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6805 XbaI T'CTAG_A Cuts at: 4141 4141 Size: 6805 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2267 2267 Size: 6805 XhoI C'TCGA_G Cuts at: 1943 2370 1943 Size: 427 6378 XmaI C'CCGG_G Cuts at: 1870 2214 4130 1870 Size: 344 1916 4545 Fragments arranged by size: 4545 1916 344 XmnI GAAnn'nnTTC Cuts at: 3143 3791 4934 3143 Size: 648 1143 5014 Fragments arranged by size: 5014 1143 648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4613 276 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SanDI SexAI SfiI SfoI SrfI SwaI