(Circular) (Six-base) MAPSORT of: pFN21AB5511.seq Check: 2107 from: 1 to: 6886 pFN21AB5511 6886 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2210 3558 2210 Size: 1348 5538 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2216 4696 278 Size: 53 83 186 936 680 2480 2468 Fragments arranged by size: 2480 2468 936 680 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2366 3124 4949 6333 6601 1632 Size: 635 99 758 1825 1384 268 1917 Fragments arranged by size: 1917 1825 1384 758 635 268 99 AfeI AGC'GCT Cuts at: 2141 2141 Size: 6886 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 5785 6762 1203 Size: 4582 977 1327 Fragments arranged by size: 4582 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 3692 1166 Size: 2526 4360 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3124 3404 1184 Size: 1940 280 4666 Fragments arranged by size: 4666 1940 280 AlwNI CAG_nnn'CTG Cuts at: 2632 4629 6201 2632 Size: 1997 1572 3317 Fragments arranged by size: 3317 1997 1572 ApaI G_GGCC'C Cuts at: 2334 3059 3910 2334 Size: 725 851 5310 Fragments arranged by size: 5310 851 725 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 3845 4195 4421 4573 6682 1493 Size: 2352 350 226 152 2109 1697 Fragments arranged by size: 2352 2109 1697 350 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 2334 3584 4211 1870 Size: 73 391 1250 627 4545 Fragments arranged by size: 4545 1250 627 391 73 AvrII C'CTAG_G Cuts at: 2182 2182 Size: 6886 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4151 4207 5662 618 Size: 325 217 627 2364 56 1455 1842 Fragments arranged by size: 2364 1842 1455 627 325 217 56 BanII G_rGCy'C Cuts at: 1391 2334 2343 2397 2465 3059 3174 3268 3910 Size: 943 9 54 68 594 115 94 642 Cuts at: 3910 4078 4137 4205 1391 Size: 168 59 68 4072 Fragments arranged by size: 4072 943 642 594 168 115 94 68 68 59 54 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 928 Size: 971 472 5443 Fragments arranged by size: 5443 971 472 BbvCI CC'TCA_GC Cuts at: 2388 2495 2388 Size: 107 6779 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5077 5111 1903 Size: 34 50 34 3056 34 3678 Fragments arranged by size: 3678 3056 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2439 4778 5988 2439 Size: 2339 1210 3337 Fragments arranged by size: 3337 2339 1210 BclI T'GATC_A Cuts at: 1548 3372 1548 Size: 1824 5062 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2838 5497 136 Size: 107 122 71 631 605 1166 2659 1525 Fragments arranged by size: 2659 1525 1166 631 605 122 107 71 BglII A'GATC_T Cuts at: 2559 3412 6881 2559 Size: 853 3469 2564 Fragments arranged by size: 3469 2564 853 BlpI GC'TnA_GC Cuts at: 2143 4303 2143 Size: 2160 4726 Bme1580I G_kGCm'C Cuts at: 1790 2334 3059 3351 3388 3910 4154 4947 6103 Size: 544 725 292 37 522 244 793 1156 Cuts at: 6103 1790 Size: 2573 Fragments arranged by size: 2573 1156 793 725 544 522 292 244 37 BmgBI CAC'GTC Cuts at: 1318 2314 3157 1318 Size: 996 843 5047 Fragments arranged by size: 5047 996 843 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3904 4617 5575 Size: 416 53 83 399 24 2487 713 958 Cuts at: 5575 442 Size: 1753 Fragments arranged by size: 2487 1753 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3973 3973 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2438 2487 3875 5546 1239 Size: 1040 159 49 1388 1671 2579 Fragments arranged by size: 2579 1671 1388 1040 159 49 Bpu10I CC'TnA_GC Cuts at: 2388 2495 2997 2388 Size: 107 502 6277 Fragments arranged by size: 6277 502 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 2819 5011 5876 6174 2645 Size: 174 2192 865 298 3357 Fragments arranged by size: 3357 2192 865 298 174 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2108 2414 3747 5549 882 Size: 617 609 306 1333 1802 2219 Fragments arranged by size: 2219 1802 1333 617 609 306 BsaAI yAC'GTr Cuts at: 493 1204 3307 4677 493 Size: 711 2103 1370 2702 Fragments arranged by size: 2702 2103 1370 711 BsaBI GATnn'nnATC Cuts at: 2594 6880 2594 Size: 4286 2600 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2213 3653 4693 Size: 53 83 186 936 534 146 1440 1040 Cuts at: 4693 5075 5710 275 Size: 382 635 1451 Fragments arranged by size: 1451 1440 1040 936 635 534 382 186 146 83 53 BsaWI w'CCGG_w Cuts at: 2431 5319 5717 5991 6138 6549 2431 Size: 2888 398 274 147 411 2768 Fragments arranged by size: 2888 2768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2411 2441 1178 Size: 30 165 30 1008 30 5623 Fragments arranged by size: 5623 1008 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 3731 1186 Size: 1219 1326 4341 Fragments arranged by size: 4341 1326 1219 BseYI C'CCAG_C Cuts at: 2032 6089 6585 2032 Size: 4057 496 2333 Fragments arranged by size: 4057 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2788 3112 3294 3338 3536 1163 Size: 573 1052 324 182 44 198 4513 Fragments arranged by size: 4513 1052 573 324 198 182 44 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2343 2397 2661 3729 4947 5032 6103 2343 Size: 54 264 1068 1218 85 1071 3126 Fragments arranged by size: 3126 1218 1071 1068 264 85 54 BsmI GAATG_Cn' Cuts at: 3082 4397 4490 3082 Size: 1315 93 5478 Fragments arranged by size: 5478 1315 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3644 3644 Size: 6886 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2334 2343 2397 2465 2661 3059 3174 Size: 399 544 9 54 68 196 398 115 Cuts at: 3174 3268 3351 3388 3729 3910 4078 4137 4154 Size: 94 83 37 341 181 168 59 17 Cuts at: 4154 4205 4947 5032 6103 1391 Size: 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 399 398 341 196 181 168 115 94 85 83 68 59 54 51 37 17 9 BspEI T'CCGG_A Cuts at: 6549 6549 Size: 6886 BspHI T'CATG_A Cuts at: 4775 6505 4775 Size: 1730 5156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2210 3558 4241 844 Size: 1366 1348 683 3489 Fragments arranged by size: 3489 1366 1348 683 BsrBI CCG'CTC Cuts at: 1394 1823 3832 4773 1394 Size: 429 2009 941 3507 Fragments arranged by size: 3507 2009 941 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3213 3861 5381 5555 65 Size: 1183 1965 648 1520 174 1396 Fragments arranged by size: 1965 1520 1396 1183 648 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3111 3498 5530 5717 1700 Size: 110 1301 387 2032 187 2869 Fragments arranged by size: 2869 2032 1301 387 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 2978 4946 5958 2978 Size: 1968 1012 3906 Fragments arranged by size: 3906 1968 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6886 BstBI TT'CG_AA Cuts at: 2793 5690 2793 Size: 2897 3989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 2177 2869 3094 3631 2177 Size: 692 225 537 5432 Fragments arranged by size: 5432 692 537 225 BstYI r'GATC_y Cuts at: 1972 2166 2281 2559 3412 4216 4583 4968 4985 Size: 194 115 278 853 804 367 385 17 Cuts at: 4985 6437 6523 6535 6881 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 853 804 385 367 346 278 194 115 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3060 3060 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 2725 2862 513 Size: 552 1660 137 4537 Fragments arranged by size: 4537 1660 552 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3985 4147 487 Size: 683 56 2759 162 3226 Fragments arranged by size: 3226 2759 683 162 56 BtsI GCAGTG_nn' Cuts at: 3388 4402 5196 5216 3388 Size: 1014 794 20 5058 Fragments arranged by size: 5058 1014 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2884 2919 529 Size: 35 2320 35 4496 Fragments arranged by size: 4496 2320 35 35 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DraIII CAC_nnn'GTG Cuts at: 2811 2811 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2103 2508 Size: 54 1425 50 275 18 219 54 405 Cuts at: 2508 3511 3733 4333 5222 6851 8 Size: 1003 222 600 889 1629 43 Fragments arranged by size: 1629 1425 1003 889 600 405 275 222 219 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2573 3000 3422 4816 1353 Size: 947 273 427 422 1394 3423 Fragments arranged by size: 3423 1394 947 427 422 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5473 5847 5993 387 Size: 1064 138 3884 374 146 1280 Fragments arranged by size: 3884 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2341 2395 2341 Size: 54 6832 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2279 2366 2438 2487 3124 3875 Size: 393 635 12 87 72 49 637 751 Cuts at: 3875 4949 5546 6333 6601 1239 Size: 1074 597 787 268 1524 Fragments arranged by size: 1524 1074 787 751 637 635 597 393 268 87 72 49 12 EcoNI CCTnn'n_nnAGG Cuts at: 2186 3566 2186 Size: 1380 5506 EcoO109I rG'GnC_Cy Cuts at: 2330 2331 3056 3906 2330 Size: 1 725 850 5310 Fragments arranged by size: 5310 850 725 1 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6886 FspI TGC'GCA Cuts at: 2828 5392 2828 Size: 2564 4322 HaeII r_GCGC'y Cuts at: 1678 2143 2177 6033 1678 Size: 465 34 3856 2531 Fragments arranged by size: 3856 2531 465 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2413 2445 2460 2492 2952 Size: 32 135 32 841 32 15 32 460 Cuts at: 2952 2984 3023 3055 3389 3421 5527 5559 5601 Size: 32 39 32 334 32 2106 32 42 Cuts at: 5601 5633 1373 Size: 32 2626 Fragments arranged by size: 2626 2106 841 460 334 135 42 39 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 4230 4476 669 Size: 988 281 2292 246 3079 Fragments arranged by size: 3079 2292 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6886 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 4485 4485 Size: 6886 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3220 6000 6184 1305 Size: 1915 2780 184 2007 Fragments arranged by size: 2780 2007 1915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 3513 3735 10 Size: 54 1425 343 273 1408 222 3161 Fragments arranged by size: 3161 1425 1408 343 273 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 3809 3975 4125 4844 5203 5362 Size: 648 2526 117 166 150 719 359 159 Cuts at: 5362 518 Size: 2042 Fragments arranged by size: 2526 2042 719 648 359 166 159 150 117 MspA1I CmG'CkG Cuts at: 1606 2545 2613 2923 3126 3223 4302 4979 6127 Size: 939 68 310 203 97 1079 677 1148 Cuts at: 6127 6372 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1079 939 677 310 245 203 97 68 NaeI GCC'GGC Cuts at: 3113 3113 Size: 6886 NcoI C'CATG_G Cuts at: 513 1065 2725 513 Size: 552 1660 4674 Fragments arranged by size: 4674 1660 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NgoMIV G'CCGG_C Cuts at: 3111 3111 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 2959 4244 5789 1132 Size: 97 1730 1285 1545 2229 Fragments arranged by size: 2229 1730 1545 1285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 5785 5785 Size: 6886 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 1108 Size: 1524 5362 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PspOMI G'GGCC_C Cuts at: 2330 3055 3906 2330 Size: 725 851 5310 Fragments arranged by size: 5310 851 725 PstI C_TGCA'G Cuts at: 830 2751 3405 4238 830 Size: 1921 654 833 3478 Fragments arranged by size: 3478 1921 833 654 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 2545 3126 2545 Size: 581 6305 SacI G_AGCT'C Cuts at: 2343 2397 2343 Size: 54 6832 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 2747 3401 4234 5369 6050 6241 826 Size: 219 1702 654 833 1135 681 191 1471 Fragments arranged by size: 1702 1471 1135 833 681 654 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 2336 4213 1872 Size: 464 1877 4545 Fragments arranged by size: 4545 1877 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 2834 4990 5891 6153 820 Size: 197 926 717 174 2156 901 262 1553 Fragments arranged by size: 2156 1553 926 901 717 262 197 174 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2182 2643 2725 3508 4079 4325 513 Size: 552 1117 461 82 783 571 246 3074 Fragments arranged by size: 3074 1117 783 571 552 461 246 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 3182 3560 4913 4930 5083 5268 Size: 1255 552 1050 378 1353 17 153 185 Cuts at: 5268 5713 325 Size: 445 1498 Fragments arranged by size: 1498 1353 1255 1050 552 445 378 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3603 3858 5132 Size: 274 80 33 51 1443 1626 255 1274 Cuts at: 5132 6844 96 Size: 1712 138 Fragments arranged by size: 1712 1626 1443 1274 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6846 6878 6846 Size: 32 6854 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2431 2563 2633 4086 2371 Size: 60 132 70 1453 5171 Fragments arranged by size: 5171 1453 132 70 60 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 2334 4211 1870 Size: 464 1877 4545 Fragments arranged by size: 4545 1877 464 XmnI GAAnn'nnTTC Cuts at: 2351 5015 2351 Size: 2664 4222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2214 4694 276 Size: 53 83 186 936 680 2480 2468 Fragments arranged by size: 2480 2468 936 680 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI