Restriction map for
pFN21AB5418
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsoBI
Bsp1407I BspEI BspHI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FbaI FseI FspI HaeIII
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NcoI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuII
RcaI RsrII SacI SacII SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 558 ACATGT A^C[GA][CT]GT
Aor51HI 125 AGCGCT AGC^GCT
Asp700I 1174 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1037 GAGCAC G[AT]GC[AT]^C
AvrII 1059 CCTAGG C^CTAGG
BbsI 309 GTCTTC GAAGAC(2/6)
BfaI 1060 CTAG C^TAG
BlnI 1059 CCTAGG C^CTAGG
BpmI 1086 CTGGAG CTGGAG(16/14)
BpuAI 309 GTCTTC GAAGAC(2/6)
BsaI 272 GAGACC GGTCTC(1/5)
BsgI 889 GTGCAG GTGCAG(16/14)
BsiEI 1242 CGATCG CG[GA][CT]^CG
BsiHKAI 1037 GAGCAC G[AT]GC[AT]^C
BsmI 1100 GCATTC GAATGC(1/-1)
BspLU11I 558 ACATGT A^CATGT
BspMI 176 GCAGGT ACCTGC(4/8)
BsrDI 1165 GCAATG GCAATG(2/0)
BstAPI 1091 GCATAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 439 GGTCACC G^GT[ACGT]ACC
BstPI 439 GGTCACC G^GT[ACGT]ACC
CfoI 126 GCGC GCG^C
Eco47III 125 AGCGCT AGC^GCT
EcoO65I 439 GGTCACC G^GT[ACGT]ACC
EcoT14I 1059 CCTAGG C^C[AT][AT]GG
EcoT22I 1098 ATGCAT ATGCA^T
FauI 547 GCGGG CCCGC(4/6)
HaeII 125 AGCGCT [GA]GCGC^[CT]
HhaI 126 GCGC GCG^C
HinP1I 126 GCGC G^CGC
MaeI 1060 CTAG C^TAG
MslI 103 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 666 CCGCTG C[AC]G^C[GT]G
MwoI 1091 GCATAGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 1273 GCCGGC GCC^GGC
NgoMIV 1273 GCCGGC G^CCGGC
NlaIV 1067 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 1098 ATGCAT ATGCA^T
NspI 558 ACATGT [GA]CATG^[CT]
PvuI 1242 CGATCG CGAT^CG
SalI 206 GTCGAC G^TCGAC
SspI 1229 AATATT AAT^ATT
StyI 1059 CCTAGG C^C[AT][AT]GG
TspRI 108 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 1075 CCATGTGATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1174 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 666 CCGC CCGC(-3/-1)
547 GCGG
AcsI 828 GAATTT [GA]^AATT[CT]
1312 GAATTT
AluI 510 AGCT AG^CT
603 AGCT
AlwI 1381 GATCC GGATC(4/5)
786 GGATC
ApoI 828 GAATTT [GA]^AATT[CT]
1312 GAATTT
AvaII 1067 GGTCC G^G[AT]CC
1186 GGACC
BmyI 110 GTGCCC G[AGT]GC[ACT]^C
1037 GAGCAC
BsaJI 113 CCCAGG C^C[ACGT][ACGT]GG
1059 CCTAGG
BseRI 66 GAGGAG GAGGAG(10/8)
433 GAGGAG
BsmBI 1345 CGTCTC CGTCTC(1/5)
461 GAGACG
BsmFI 732 GGGAC GGGAC(10/14)
1068 GTCCC
Bsp1286I 110 GTGCCC G[AGT]GC[ACT]^C
1037 GAGCAC
BspDI 535 ATCGAT AT^CGAT
1244 ATCGAT
BsrFI 284 GCCGGT [GA]^CCGG[CT]
1273 GCCGGC
BsrI 783 ACTGG ACTGG(1/-1)
1253 ACTGG
Cac8I 607 GCTCGC GC[ACGT]^[ACGT]GC
1273 GCCGGC
Cfr10I 284 GCCGGT [GA]^CCGG[CT]
1273 GCCGGC
Cfr13I 1067 GGTCC G^G[ACGT]CC
1186 GGACC
ClaI 535 ATCGAT AT^CGAT
1244 ATCGAT
EarI 93 GAAGAG CTCTTC(1/4)
390 GAAGAG
HapII 285 CCGG C^CGG
1274 CCGG
HincII 206 GTCGAC GT[CT]^[GA]AC
849 GTTGAC
HindII 206 GTCGAC GT[CT]^[GA]AC
849 GTTGAC
HpaII 285 CCGG C^CGG
1274 CCGG
Ksp632I 93 GAAGAG CTCTTC(1/4)
390 GAAGAG
MspI 285 CCGG C^CGG
1274 CCGG
NdeI 989 CATATG CA^TATG
1201 CATATG
PflMI 162 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
675 CCAGATGTTGG
PleI 852 GACTC GAGTC(4/5)
393 GAGTC
Sau96I 1067 GGTCC G^G[ACGT]CC
1186 GGACC
SmlI 242 CTCAAG C^T[CT][GA]AG
1325 CTCAAG
TfiI 354 GATTC G^A[AT]TC
1179 GATTC
Van91I 162 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
675 CCAGATGTTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccI AfaI BbvI BsiYI BslI
BsmAI BssKI BstF5I BstNI DdeI
DpnII EcoRII Fnu4HI FokI HgaI
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII RsaI Sau3AI ScaI
ScrFI SfaNI TaqI Tru9I TseI
Tsp45I Tsp509I TthHB8I