(Circular) (Six-base) MAPSORT of: pFN21AB5418.seq Check: 6961 from: 1 to: 6157 pFN21AB5418 6157 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3967 278 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2212 2560 2976 3500 1937 Size: 275 348 416 524 4594 Fragments arranged by size: 4594 524 416 348 275 Acc65I G'GTAC_C Cuts at: 3478 3478 Size: 6157 AclI AA'CG_TT Cuts at: 1563 4284 4657 1563 Size: 2721 373 3063 Fragments arranged by size: 3063 2721 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3069 3332 3464 4220 5604 5872 1632 Size: 1437 263 132 756 1384 268 1917 Fragments arranged by size: 1917 1437 1384 756 268 263 132 AfeI AGC'GCT Cuts at: 2132 2132 Size: 6157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5960 AflIII A'CryG_T Cuts at: 1203 2563 5056 6033 1203 Size: 1360 2493 977 1327 Fragments arranged by size: 2493 1360 1327 977 AgeI A'CCGG_T Cuts at: 4988 4988 Size: 6157 AhdI GACnn_n'nnGTC Cuts at: 4886 4886 Size: 6157 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6157 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2014 2046 2014 Size: 32 6125 AlwNI CAG_nnn'CTG Cuts at: 3900 5472 3900 Size: 1572 4585 ApaLI G'TGCA_C Cuts at: 4214 5370 4214 Size: 1156 5001 ApoI r'AATT_y Cuts at: 1493 2833 3317 3466 3692 3844 5953 1493 Size: 1340 484 149 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 484 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 4711 160 Size: 872 3679 1606 Fragments arranged by size: 3679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 AvaI C'yCGr_G Cuts at: 1870 1943 3482 1870 Size: 73 1539 4545 Fragments arranged by size: 4545 1539 73 AvrII C'CTAG_G Cuts at: 3064 3064 Size: 6157 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3006 3039 3006 Size: 33 6124 BamHI G'GATC_C Cuts at: 3487 3854 3487 Size: 367 5790 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3478 4933 618 Size: 325 217 627 1691 1455 1842 Fragments arranged by size: 1842 1691 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3476 1391 Size: 2085 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2307 928 Size: 971 408 4778 Fragments arranged by size: 4778 971 408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4348 4382 1903 Size: 34 2411 34 3678 Fragments arranged by size: 3678 2411 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4049 5259 4049 Size: 1210 4947 BclI T'GATC_A Cuts at: 1548 1548 Size: 6157 BfrBI ATG'CAT Cuts at: 3105 3105 Size: 6157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4768 136 Size: 107 122 71 631 605 3096 1525 Fragments arranged by size: 3096 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6152 6152 Size: 6157 BlpI GC'TnA_GC Cuts at: 3574 3574 Size: 6157 Bme1580I G_kGCm'C Cuts at: 1790 2119 4218 5374 1790 Size: 329 2099 1156 2573 Fragments arranged by size: 2573 2099 1156 329 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6157 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3268 3888 4846 Size: 416 53 83 399 24 1851 620 958 Cuts at: 4846 442 Size: 1753 Fragments arranged by size: 1851 1753 958 620 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3112 4817 1239 Size: 1873 1705 2579 Fragments arranged by size: 2579 1873 1705 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2232 3315 4282 5147 5445 2232 Size: 1083 967 865 298 2944 Fragments arranged by size: 2944 1083 967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2271 4820 882 Size: 617 772 2549 2219 Fragments arranged by size: 2549 2219 772 617 BsaAI yAC'GTr Cuts at: 493 1204 3948 493 Size: 711 2744 2702 Fragments arranged by size: 2744 2702 711 BsaBI GATnn'nnATC Cuts at: 6151 6151 Size: 6157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3964 4346 4981 275 Size: 53 83 186 936 2431 382 635 1451 Fragments arranged by size: 2431 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4590 4988 5262 5409 5820 4590 Size: 398 274 147 411 4927 Fragments arranged by size: 4927 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5932 Fragments arranged by size: 5932 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2086 2453 1186 Size: 900 367 4890 Fragments arranged by size: 4890 900 367 BseYI C'CCAG_C Cuts at: 5360 5856 5360 Size: 496 5661 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 1163 Size: 573 1179 4405 Fragments arranged by size: 4405 1179 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 3607 4368 5396 1540 Size: 275 186 1249 357 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 357 275 186 BsiHKAI G_wGCw'C Cuts at: 3046 4218 4303 5374 3046 Size: 1172 85 1071 3829 Fragments arranged by size: 3829 1172 1071 85 BsmI GAATG_Cn' Cuts at: 3105 3668 3761 3105 Size: 563 93 5501 Fragments arranged by size: 5501 563 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2460 3356 2460 Size: 896 5261 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 3046 3476 4218 4303 5374 1391 Size: 399 329 927 430 742 85 1071 2174 Fragments arranged by size: 2174 1071 927 742 430 399 329 85 BspEI T'CCGG_A Cuts at: 5820 5820 Size: 6157 BspHI T'CATG_A Cuts at: 4046 5776 4046 Size: 1730 4427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 3512 844 Size: 1328 1340 3489 Fragments arranged by size: 3489 1340 1328 BsrBI CCG'CTC Cuts at: 1394 1823 4044 1394 Size: 429 2221 3507 Fragments arranged by size: 3507 2221 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3177 4652 4826 65 Size: 1183 1929 1475 174 1396 Fragments arranged by size: 1929 1475 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2289 3278 4801 4988 1700 Size: 110 479 989 1523 187 2869 Fragments arranged by size: 2869 1523 989 479 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6157 BssSI C'ACGA_G Cuts at: 4217 5229 4217 Size: 1012 5145 BstAPI GCAn_nnn'nTGC Cuts at: 794 3102 794 Size: 2308 3849 BstBI TT'CG_AA Cuts at: 4961 4961 Size: 6157 BstEII G'GTnAC_C Cuts at: 1184 2444 1184 Size: 1260 4897 BstYI r'GATC_y Cuts at: 1972 3487 3854 4239 4256 5708 5794 5806 6152 Size: 1515 367 385 17 1452 86 12 346 Cuts at: 6152 1972 Size: 1977 Fragments arranged by size: 1977 1515 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5605 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2252 487 Size: 683 56 1026 4392 Fragments arranged by size: 4392 1026 683 56 BtsI GCAGTG_nn' Cuts at: 2119 3673 4467 4487 2119 Size: 1554 794 20 3789 Fragments arranged by size: 3789 1554 794 20 ClaI AT'CG_AT Cuts at: 1554 2541 3250 1554 Size: 987 709 4461 Fragments arranged by size: 4461 987 709 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6122 DraI TTT'AAA Cuts at: 3461 3461 Size: 6157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5164 809 Size: 507 3848 1802 Fragments arranged by size: 3848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3604 4493 6122 Size: 54 1425 50 275 18 1774 889 1629 Cuts at: 6122 8 Size: 43 Fragments arranged by size: 1774 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3604 1537 Size: 275 1792 4090 Fragments arranged by size: 4090 1792 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2390 4087 1353 Size: 740 297 1697 3423 Fragments arranged by size: 3423 1697 740 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4744 5118 5264 387 Size: 1064 138 3155 374 146 1280 Fragments arranged by size: 3155 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3069 3112 3332 3464 4220 4817 5604 Size: 393 1437 43 220 132 756 597 787 Cuts at: 5604 5872 1239 Size: 268 1524 Fragments arranged by size: 1524 1437 787 756 597 393 268 220 132 43 EcoRI G'AATT_C Cuts at: 3466 3844 3466 Size: 378 5779 FspI TGC'GCA Cuts at: 4663 4663 Size: 6157 HaeII r_GCGC'y Cuts at: 1678 2134 5304 1678 Size: 456 3170 2531 Fragments arranged by size: 3170 2531 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2797 2829 4798 4830 4872 Size: 32 135 32 1225 32 1969 32 42 Cuts at: 4872 4904 1373 Size: 32 2626 Fragments arranged by size: 2626 1969 1225 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2213 2856 3501 3747 669 Size: 988 281 275 643 645 246 3079 Fragments arranged by size: 3079 988 645 643 281 275 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6157 HpaI GTT'AAC Cuts at: 3747 3747 Size: 6157 KpnI G_GTAC'C Cuts at: 3482 3482 Size: 6157 MfeI C'AATT_G Cuts at: 3756 3756 Size: 6157 MluI A'CGCG_T Cuts at: 6033 6033 Size: 6157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2667 3167 3357 5271 5455 1305 Size: 1362 500 190 1914 184 2007 Fragments arranged by size: 2007 1914 1362 500 190 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4335 Fragments arranged by size: 4335 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 4115 4474 4633 518 Size: 648 946 2003 359 159 2042 Fragments arranged by size: 2042 2003 946 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2673 3573 4250 5398 5643 1606 Size: 1067 900 677 1148 245 2120 Fragments arranged by size: 2120 1148 1067 900 677 245 NaeI GCC'GGC Cuts at: 3280 3280 Size: 6157 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5605 NdeI CA'TA_TG Cuts at: 387 2995 3207 387 Size: 2608 212 3337 Fragments arranged by size: 3337 2608 212 NgoMIV G'CCGG_C Cuts at: 3278 3278 Size: 6157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6157 NotI GC'GGCC_GC Cuts at: 3604 3604 Size: 6157 NsiI A_TGCA'T Cuts at: 3107 3107 Size: 6157 NspI r_CATG'y Cuts at: 1132 1229 2567 3515 5060 1132 Size: 97 1338 948 1545 2229 Fragments arranged by size: 2229 1545 1338 948 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6157 PciI A'CATG_T Cuts at: 2563 5056 2563 Size: 2493 3664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2173 2686 1108 Size: 1065 513 4579 Fragments arranged by size: 4579 1065 513 PmeI GTTT'AAAC Cuts at: 3461 3461 Size: 6157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4226 4258 5739 5771 4226 Size: 32 1481 32 4612 Fragments arranged by size: 4612 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6157 PsiI TTA'TAA Cuts at: 2343 3727 2343 Size: 1384 4773 PstI C_TGCA'G Cuts at: 830 3509 830 Size: 2679 3478 PvuI CG_AT'CG Cuts at: 2001 3250 2001 Size: 1249 4908 SalI G'TCGA_C Cuts at: 1936 2211 3499 1936 Size: 275 1288 4594 Fragments arranged by size: 4594 1288 275 SbfI CC_TGCA'GG Cuts at: 3509 3509 Size: 6157 ScaI AGT'ACT Cuts at: 2371 2665 2746 4405 6117 2371 Size: 294 81 1659 1712 2411 Fragments arranged by size: 2411 1712 1659 294 81 SfcI C'TryA_G Cuts at: 826 1045 3505 4640 5321 5512 826 Size: 219 2460 1135 681 191 1471 Fragments arranged by size: 2460 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6157 SmaI CCC'GGG Cuts at: 1872 3484 1872 Size: 1612 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2247 3330 4261 5162 5424 820 Size: 197 926 304 1083 931 901 262 1553 Fragments arranged by size: 1553 1083 931 926 901 304 262 197 SnaBI TAC'GTA Cuts at: 493 3948 493 Size: 3455 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6157 SphI G_CATG'C Cuts at: 1132 3515 1132 Size: 2383 3774 SspI AAT'ATT Cuts at: 5 52 3236 4081 5 Size: 47 3184 845 2081 Fragments arranged by size: 3184 2081 845 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6157 StyI C'CwwG_G Cuts at: 513 1065 3064 3596 513 Size: 552 1999 532 3074 Fragments arranged by size: 3074 1999 552 532 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4184 4201 4354 4539 4984 325 Size: 1255 2604 17 153 185 445 1498 Fragments arranged by size: 2604 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 2369 2663 Size: 274 80 33 51 1443 252 140 294 Cuts at: 2663 2744 3014 4403 6115 96 Size: 81 270 1389 1712 138 Fragments arranged by size: 1712 1443 1389 294 274 270 252 140 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 6117 6149 2514 Size: 32 3571 32 2522 Fragments arranged by size: 3571 2522 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6157 XbaI T'CTAG_A Cuts at: 3493 3493 Size: 6157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3087 3087 Size: 6157 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6157 XmaI C'CCGG_G Cuts at: 1870 3482 1870 Size: 1612 4545 XmnI GAAnn'nnTTC Cuts at: 3183 4286 3183 Size: 1103 5054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3965 276 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI