Restriction map for
pFN21AB5418
(Linear) (Six-base) MAPSORT of: KIBB5418.seq Check: 7863 from: 1 to: 1452
KIBB5418.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 207 555 971 1452
Size: 207 348 416 481
Fragments arranged by size:
481 416 348 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1327 1452
Size: 1064 263 125
Fragments arranged by size:
1064 263 125
AfeI AGC'GCT
Cuts at: 0 127 1452
Size: 127 1325
AflIII A'CryG_T
Cuts at: 0 558 1452
Size: 558 894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 9 41 1452
Size: 9 32 1411
Fragments arranged by size:
1411 32 9
ApoI r'AATT_y
Cuts at: 0 828 1312 1452
Size: 828 484 140
Fragments arranged by size:
828 484 140
AvrII C'CTAG_G
Cuts at: 0 1059 1452
Size: 1059 393
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1001 1034 1452
Size: 1001 33 418
Fragments arranged by size:
1001 418 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 1452
Size: 302 1150
BfrBI ATG'CAT
Cuts at: 0 1100 1452
Size: 1100 352
Bme1580I G_kGCm'C
Cuts at: 0 114 1452
Size: 114 1338
BmrI ACTGGGnnnn_n'
Cuts at: 0 1263 1452
Size: 1263 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1107 1452
Size: 1107 345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1310 1452
Size: 227 1083 142
Fragments arranged by size:
1083 227 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 1452
Size: 266 1186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 448 1452
Size: 81 367 1004
Fragments arranged by size:
1004 367 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1452
Size: 910 542
BsiEI CG_ry'CG
Cuts at: 0 1245 1452
Size: 1245 207
BsiHKAI G_wGCw'C
Cuts at: 0 1041 1452
Size: 1041 411
BsmI GAATG_Cn'
Cuts at: 0 1100 1452
Size: 1100 352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 455 1351 1452
Size: 455 896 101
Fragments arranged by size:
896 455 101
Bsp1286I G_dGCh'C
Cuts at: 0 114 1041 1452
Size: 114 927 411
Fragments arranged by size:
927 411 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 1452
Size: 167 1285
BsrDI GCAATG_nn'
Cuts at: 0 1172 1452
Size: 1172 280
BsrFI r'CCGG_y
Cuts at: 0 284 1273 1452
Size: 284 989 179
Fragments arranged by size:
989 284 179
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1097 1452
Size: 1097 355
BstEII G'GTnAC_C
Cuts at: 0 439 1452
Size: 439 1013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 247 1452
Size: 247 1205
BtsI GCAGTG_nn'
Cuts at: 0 114 1452
Size: 114 1338
ClaI AT'CG_AT
Cuts at: 0 536 1245 1452
Size: 536 709 207
Fragments arranged by size:
709 536 207
EarI CTCTTCn'nnn_
Cuts at: 0 88 385 1452
Size: 88 297 1067
Fragments arranged by size:
1067 297 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1107 1327 1452
Size: 1064 43 220 125
Fragments arranged by size:
1064 220 125 43
HaeII r_GCGC'y
Cuts at: 0 129 1452
Size: 129 1323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 792 824 1452
Size: 792 32 628
Fragments arranged by size:
792 628 32
HincII GTy'rAC
Cuts at: 0 208 851 1452
Size: 208 643 601
Fragments arranged by size:
643 601 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 1162 1352 1452
Size: 662 500 190 100
Fragments arranged by size:
662 500 190 100
MslI CAynn'nnrTG
Cuts at: 0 107 1452
Size: 107 1345
MspA1I CmG'CkG
Cuts at: 0 668 1452
Size: 668 784
NaeI GCC'GGC
Cuts at: 0 1275 1452
Size: 1275 177
NdeI CA'TA_TG
Cuts at: 0 990 1202 1452
Size: 990 212 250
Fragments arranged by size:
990 250 212
NgoMIV G'CCGG_C
Cuts at: 0 1273 1452
Size: 1273 179
NsiI A_TGCA'T
Cuts at: 0 1102 1452
Size: 1102 350
NspI r_CATG'y
Cuts at: 0 562 1452
Size: 562 890
PciI A'CATG_T
Cuts at: 0 558 1452
Size: 558 894
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 681 1452
Size: 168 513 771
Fragments arranged by size:
771 513 168
PsiI TTA'TAA
Cuts at: 0 338 1452
Size: 338 1114
PvuI CG_AT'CG
Cuts at: 0 1245 1452
Size: 1245 207
SalI G'TCGA_C
Cuts at: 0 206 1452
Size: 206 1246
ScaI AGT'ACT
Cuts at: 0 366 660 741 1452
Size: 366 294 81 711
Fragments arranged by size:
711 366 294 81
SmlI C'TyrA_G
Cuts at: 0 242 1325 1452
Size: 242 1083 127
Fragments arranged by size:
1083 242 127
SspI AAT'ATT
Cuts at: 0 1231 1452
Size: 1231 221
StyI C'CwwG_G
Cuts at: 0 1059 1452
Size: 1059 393
TatI w'GTAC_w
Cuts at: 0 224 364 658 739 1009 1452
Size: 224 140 294 81 270 443
Fragments arranged by size:
443 294 270 224 140 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 1452
Size: 509 32 911
Fragments arranged by size:
911 509 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1082 1452
Size: 1082 370
XmnI GAAnn'nnTTC
Cuts at: 0 1178 1452
Size: 1178 274
Enzymes that do cut:
AccI AcuI AfeI AflIII AloI ApoI AvrII BaeI
BbsI BfrBI Bme1580I BmrI BpmI BpuEI BsaI BseRI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstEII BtgZI BtsI ClaI EarI Eco57MI
HaeII Hin4I HincII MmeI MslI MspA1I NaeI NdeI
NgoMIV NsiI NspI PciI PflMI PsiI PvuI SalI
ScaI SmlI SspI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NcoI NheI NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuII RsrII SacI SacII SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI