Restriction map for pFN21AB5300
 (Circular) (Six-base) MAPSORT of: pFN21AB5300.seq  Check: 781  from: 1  to: 5956



pFN21AB5300  5956 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3766     278 
   Size:         53      83     186     936    2230    2468

  Fragments arranged by size: 

                2468    2230     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2073    3299    1937 
   Size:        136    1226    4594

  Fragments arranged by size: 

                4594    1226     136
 
Acc65I G'GTAC_C

Cuts at:   3277    3277 
   Size:       5956
 
AclI AA'CG_TT

Cuts at:   1563    4083    4456    1563 
   Size:       2520     373    3063

  Fragments arranged by size: 

                3063    2520     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    4019    5403    5671    1632 
   Size:       2387    1384     268    1917

  Fragments arranged by size: 

                2387    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2738     820 
   Size:        197    1721    4038

  Fragments arranged by size: 

                4038    1721     197
 
AflIII A'CryG_T

Cuts at:   1203    4855    5832    1203 
   Size:       3652     977    1327

  Fragments arranged by size: 

                3652    1327     977
 
AgeI A'CCGG_T

Cuts at:   4787    4787 
   Size:       5956
 
AhdI GACnn_n'nnGTC

Cuts at:   2812    4685    2812 
   Size:       1873    4083
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5956
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5956
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2270    2302    2270 
   Size:         32    5924
 
AlwNI CAG_nnn'CTG

Cuts at:   2249    2342    3699    5271    2249 
   Size:         93    1357    1572    2934

  Fragments arranged by size: 

                2934    1572    1357      93
 
ApaLI G'TGCA_C

Cuts at:   4013    5169    4013 
   Size:       1156    4800
 
ApoI r'AATT_y

Cuts at:   1493    2147    3265    3491    3643    5752    1493 
   Size:        654    1118     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1118     654     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4510     160 
   Size:        872    3478    1606

  Fragments arranged by size: 

                3478    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5956
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2991    3051    3281    1870 
   Size:         73    1048      60     230    4545

  Fragments arranged by size: 

                4545    1048     230      73      60
 
AvrII C'CTAG_G

Cuts at:   2194    2194 
   Size:       5956
 
BamHI G'GATC_C

Cuts at:   3286    3653    3286 
   Size:        367    5589
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3194    3277    4732     618 
   Size:        325     217     627    1407      83    1455    1842

  Fragments arranged by size: 

                1842    1455    1407     627     325     217      83
 
BanII G_rGCy'C

Cuts at:   1391    2521    2615    3210    3275    1391 
   Size:       1130      94     595      65    4072

  Fragments arranged by size: 

                4072    1130     595      94      65
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4985
 
BbvCI CC'TCA_GC

Cuts at:   2979    2979 
   Size:       5956
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4147    4181    1903 
   Size:         34    2210      34    3678

  Fragments arranged by size: 

                3678    2210      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3848    5058    3848 
   Size:       1210    4746
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5956
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2196    2912    4567 
   Size:        107     122      71     631     605     524     716    1655

Cuts at:   4567     136 
   Size:       1525

  Fragments arranged by size: 

                1655    1525     716     631     605     524     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5951    5951 
   Size:       5956
 
BlpI GC'TnA_GC

Cuts at:   2997    3373    2997 
   Size:        376    5580
 
Bme1580I G_kGCm'C

Cuts at:   1790    2217    4017    5173    1790 
   Size:        427    1800    1156    2573

  Fragments arranged by size: 

                2573    1800    1156     427
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5956
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3195    3687    4645 
   Size:        416      53      83     399      24    1778     492     958

Cuts at:   4645     442 
   Size:       1753

  Fragments arranged by size: 

                1778    1753     958     492     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5956
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2381    4616    1239 
   Size:       1142    2235    2579

  Fragments arranged by size: 

                2579    2235    1142
 
Bpu10I CC'TnA_GC

Cuts at:   2979    2979 
   Size:       5956
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4081    4946    5244    4081 
   Size:        865     298    4793

  Fragments arranged by size: 

                4793     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4619     882 
   Size:        617    3120    2219

  Fragments arranged by size: 

                3120    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3747     493 
   Size:        711    2543    2702

  Fragments arranged by size: 

                2702    2543     711
 
BsaBI GATnn'nnATC

Cuts at:   5950    5950 
   Size:       5956
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3763    4145    4780     275 
   Size:         53      83     186     936    2230     382     635    1451

  Fragments arranged by size: 

                2230    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4389    4787    5061    5208    5619    4389 
   Size:        398     274     147     411    4726

  Fragments arranged by size: 

                4726     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2216    2246    2637    2667    1178 
   Size:         30     165      30     813      30     391      30    4467

  Fragments arranged by size: 

                4467     813     391     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5956
 
BseYI C'CCAG_C

Cuts at:   2269    2335    2342    5159    5655    2269 
   Size:         66       7    2817     496    2570

  Fragments arranged by size: 

                2817    2570     496      66       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5383
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3406    4167    5195    1540 
   Size:        275     186    1405     761    1028    2301

  Fragments arranged by size: 

                2301    1405    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2748    3185    3210    4017    4102    5173    2748 
   Size:        437      25     807      85    1071    3531

  Fragments arranged by size: 

                3531    1071     807     437      85      25
 
BsmI GAATG_Cn'

Cuts at:   2893    3467    3560    2893 
   Size:        574      93    5289

  Fragments arranged by size: 

                5289     574      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2217    2521    2615    2748    3185    3210    3275 
   Size:        399     427     304      94     133     437      25      65

Cuts at:   3275    4017    4102    5173    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     437     427     399     304     133
                  94      85      65      25
 
BspEI T'CCGG_A

Cuts at:   5619    5619 
   Size:       5956
 
BspHI T'CATG_A

Cuts at:   3845    5575    3845 
   Size:       1730    4226
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2935    3311     844 
   Size:       2091     376    3489

  Fragments arranged by size: 

                3489    2091     376
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3843    1394 
   Size:        429    2020    3507

  Fragments arranged by size: 

                3507    2020     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4451    4625      65 
   Size:       1183    3203     174    1396

  Fragments arranged by size: 

                3203    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2199    4600    4787    1700 
   Size:        110     389    2401     187    2869

  Fragments arranged by size: 

                2869    2401     389     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5956
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5956
 
BssSI C'ACGA_G

Cuts at:   4016    5028    4016 
   Size:       1012    4944
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5956
 
BstBI TT'CG_AA

Cuts at:   4760    4760 
   Size:       5956
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5956
 
BstYI r'GATC_y

Cuts at:   1972    2664    3286    3653    4038    4055    5507    5593    5605 
   Size:        692     622     367     385      17    1452      86      12

Cuts at:   5605    5951    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     692     622     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2529     513 
   Size:        552    1464    3940

  Fragments arranged by size: 

                3940    1464     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5217

  Fragments arranged by size: 

                5217     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2505    2865    3129    3472    4266    4286    2505 
   Size:        360     264     343     794      20    4175

  Fragments arranged by size: 

                4175     794     360     343     264      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5956
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5921
 
DraI TTT'AAA

Cuts at:   3260    3260 
   Size:       5956
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4963     809 
   Size:        507    3647    1802

  Fragments arranged by size: 

                3647    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2631    2899    3403 
   Size:         54    1425      50     275      18     801     268     504

Cuts at:   3403    4292    5921       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     801     504     275     268      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3403    1537 
   Size:        275    1591    4090

  Fragments arranged by size: 

                4090    1591     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2778    3886    1353 
   Size:       1425    1108    3423

  Fragments arranged by size: 

                3423    1425    1108
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2658    4543    4917    5063     387 
   Size:       1064     138    1069    1885     374     146    1280

  Fragments arranged by size: 

                1885    1280    1069    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3208    3208 
   Size:       5956
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2381    4019    4616    5403    5671    1239 
   Size:        393     749    1638     597     787     268    1524

  Fragments arranged by size: 

                1638    1524     787     749     597     393     268
 
EcoRI G'AATT_C

Cuts at:   3265    3643    3265 
   Size:        378    5578
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2251    2863    2251 
   Size:        612    5344
 
FseI GG_CCGG'CC

Cuts at:   2203    2203 
   Size:       5956
 
FspI TGC'GCA

Cuts at:   4462    4462 
   Size:       5956
 
HaeII r_GCGC'y

Cuts at:   1678    5103    1678 
   Size:       3425    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2216    2248    2798    2830    4597 
   Size:         32     135      32     644      32     550      32    1767

Cuts at:   4597    4629    4671    4703    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1767     644     550     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2074    2857    3300    3546     669 
   Size:        988     281     136     783     443     246    3079

  Fragments arranged by size: 

                3079     988     783     443     281     246     136
 
HindIII A'AGCT_T

Cuts at:    748    2448    3042     748 
   Size:       1700     594    3662

  Fragments arranged by size: 

                3662    1700     594
 
HpaI GTT'AAC

Cuts at:   3546    3546 
   Size:       5956
 
KpnI G_GTAC'C

Cuts at:   3281    3281 
   Size:       5956
 
MfeI C'AATT_G

Cuts at:   3555    3555 
   Size:       5956
 
MluI A'CGCG_T

Cuts at:   5832    5832 
   Size:       5956
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5070    5254    1305 
   Size:       3765     184    2007

  Fragments arranged by size: 

                3765    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2633    2901      10 
   Size:         54    1425     343     801     268    3065

  Fragments arranged by size: 

                3065    1425     801     343     268      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3914    4273    4432     518 
   Size:        648    2748     359     159    2042

  Fragments arranged by size: 

                2748    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2339    3372    4049    5197    5442    1606 
   Size:        733    1033     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1033     733     677     245
 
NaeI GCC'GGC

Cuts at:   2201    2201 
   Size:       5956
 
NcoI C'CATG_G

Cuts at:    513    1065    2529     513 
   Size:        552    1464    3940

  Fragments arranged by size: 

                3940    1464     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5956
 
NgoMIV G'CCGG_C

Cuts at:   2199    2199 
   Size:       5956
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5956
 
NotI GC'GGCC_GC

Cuts at:   3403    3403 
   Size:       5956
 
NspI r_CATG'y

Cuts at:   1132    1229    3314    4859    1132 
   Size:         97    2085    1545    2229

  Fragments arranged by size: 

                2229    2085    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5956
 
PciI A'CATG_T

Cuts at:   4855    4855 
   Size:       5956
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2342    1108 
   Size:       1234    4722
 
PfoI T'CCnGG_A

Cuts at:   3210    3210 
   Size:       5956
 
PmeI GTTT'AAAC

Cuts at:   3260    3260 
   Size:       5956
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3049    3081    4025    4057    5538    5570    3049 
   Size:         32     944      32    1481      32    3435

  Fragments arranged by size: 

                3435    1481     944      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2952    1613 
   Size:       1339    4617
 
PsiI TTA'TAA

Cuts at:   3526    3526 
   Size:       5956
 
PstI C_TGCA'G

Cuts at:    830    3308     830 
   Size:       2478    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5956
 
PvuII CAG'CTG

Cuts at:   2339    2339 
   Size:       5956
 
SacI G_AGCT'C

Cuts at:   3210    3210 
   Size:       5956
 
SalI G'TCGA_C

Cuts at:   1936    2072    3298    1936 
   Size:        136    1226    4594

  Fragments arranged by size: 

                4594    1226     136
 
SbfI CC_TGCA'GG

Cuts at:   3308    3308 
   Size:       5956
 
ScaI AGT'ACT

Cuts at:   2231    4204    5916    2231 
   Size:       1973    1712    2271

  Fragments arranged by size: 

                2271    1973    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2250    3304    4439    5120    5311     826 
   Size:        219    1205    1054    1135     681     191    1471

  Fragments arranged by size: 

                1471    1205    1135    1054     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5956
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5956
 
SmaI CCC'GGG

Cuts at:   1872    3053    3283    1872 
   Size:       1181     230    4545

  Fragments arranged by size: 

                4545    1181     230
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2738    4060    4961    5223     820 
   Size:        197     926     795    1322     901     262    1553

  Fragments arranged by size: 

                1553    1322     926     901     795     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3747     493 
   Size:       3254    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5956
 
SphI G_CATG'C

Cuts at:   1132    3314    1132 
   Size:       2182    3774
 
SspI AAT'ATT

Cuts at:      5      52    3253    3880       5 
   Size:         47    3201     627    2081

  Fragments arranged by size: 

                3201    2081     627      47
 
StuI AGG'CCT

Cuts at:   1517    2603    1517 
   Size:       1086    4870
 
StyI C'CwwG_G

Cuts at:    513    1065    2194    2529    2604    3395     513 
   Size:        552    1129     335      75     791    3074

  Fragments arranged by size: 

                3074    1129     791     552     335      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3983    4000    4153    4338    4783     325 
   Size:       1255    2403      17     153     185     445    1498

  Fragments arranged by size: 

                2403    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2229    4202    5914 
   Size:        274      80      33      51    1443     252    1973    1712

Cuts at:   5914      96 
   Size:        138

  Fragments arranged by size: 

                1973    1712    1443     274     252     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5916    5948    5916 
   Size:         32    5924
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2693    2861    1316 
   Size:       1377     168    4411

  Fragments arranged by size: 

                4411    1377     168
 
XbaI T'CTAG_A

Cuts at:   3292    3292 
   Size:       5956
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3067    3182    3067 
   Size:        115    5841
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5956
 
XmaI C'CCGG_G

Cuts at:   1870    3051    3281    1870 
   Size:       1181     230    4545

  Fragments arranged by size: 

                4545    1181     230
 
XmnI GAAnn'nnTTC

Cuts at:   4085    4085 
   Size:       5956
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3764     276 
   Size:         53      83     186     936    2230    2468

  Fragments arranged by size: 

                2468    2230     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FalI     FseI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SacI     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI     BaeI     BbeI    BfrBI     BplI
    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI EcoO109I
    EcoRV    FspAI     KasI     NarI     NruI     NsiI     PacI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI
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