(Circular) (Six-base) MAPSORT of: pFN21AB5300.seq Check: 781 from: 1 to: 5956 pFN21AB5300 5956 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 3299 1937 Size: 136 1226 4594 Fragments arranged by size: 4594 1226 136 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 5403 5671 1632 Size: 2387 1384 268 1917 Fragments arranged by size: 2387 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2738 820 Size: 197 1721 4038 Fragments arranged by size: 4038 1721 197 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 2812 4685 2812 Size: 1873 4083 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 2270 Size: 32 5924 AlwNI CAG_nnn'CTG Cuts at: 2249 2342 3699 5271 2249 Size: 93 1357 1572 2934 Fragments arranged by size: 2934 1572 1357 93 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2147 3265 3491 3643 5752 1493 Size: 654 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 654 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2991 3051 3281 1870 Size: 73 1048 60 230 4545 Fragments arranged by size: 4545 1048 230 73 60 AvrII C'CTAG_G Cuts at: 2194 2194 Size: 5956 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3194 3277 4732 618 Size: 325 217 627 1407 83 1455 1842 Fragments arranged by size: 1842 1455 1407 627 325 217 83 BanII G_rGCy'C Cuts at: 1391 2521 2615 3210 3275 1391 Size: 1130 94 595 65 4072 Fragments arranged by size: 4072 1130 595 94 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BbvCI CC'TCA_GC Cuts at: 2979 2979 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2196 2912 4567 Size: 107 122 71 631 605 524 716 1655 Cuts at: 4567 136 Size: 1525 Fragments arranged by size: 1655 1525 716 631 605 524 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 2997 3373 2997 Size: 376 5580 Bme1580I G_kGCm'C Cuts at: 1790 2217 4017 5173 1790 Size: 427 1800 1156 2573 Fragments arranged by size: 2573 1800 1156 427 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 3687 4645 Size: 416 53 83 399 24 1778 492 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1778 1753 958 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 4616 1239 Size: 1142 2235 2579 Fragments arranged by size: 2579 2235 1142 Bpu10I CC'TnA_GC Cuts at: 2979 2979 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2216 2246 2637 2667 1178 Size: 30 165 30 813 30 391 30 4467 Fragments arranged by size: 4467 813 391 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5956 BseYI C'CCAG_C Cuts at: 2269 2335 2342 5159 5655 2269 Size: 66 7 2817 496 2570 Fragments arranged by size: 2817 2570 496 66 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2748 3185 3210 4017 4102 5173 2748 Size: 437 25 807 85 1071 3531 Fragments arranged by size: 3531 1071 807 437 85 25 BsmI GAATG_Cn' Cuts at: 2893 3467 3560 2893 Size: 574 93 5289 Fragments arranged by size: 5289 574 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2521 2615 2748 3185 3210 3275 Size: 399 427 304 94 133 437 25 65 Cuts at: 3275 4017 4102 5173 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 437 427 399 304 133 94 85 65 25 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2935 3311 844 Size: 2091 376 3489 Fragments arranged by size: 3489 2091 376 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 4600 4787 1700 Size: 110 389 2401 187 2869 Fragments arranged by size: 2869 2401 389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2664 3286 3653 4038 4055 5507 5593 5605 Size: 692 622 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 692 622 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2529 513 Size: 552 1464 3940 Fragments arranged by size: 3940 1464 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 2505 2865 3129 3472 4266 4286 2505 Size: 360 264 343 794 20 4175 Fragments arranged by size: 4175 794 360 343 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2631 2899 3403 Size: 54 1425 50 275 18 801 268 504 Cuts at: 3403 4292 5921 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 801 504 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2778 3886 1353 Size: 1425 1108 3423 Fragments arranged by size: 3423 1425 1108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2658 4543 4917 5063 387 Size: 1064 138 1069 1885 374 146 1280 Fragments arranged by size: 1885 1280 1069 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3208 3208 Size: 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 4019 4616 5403 5671 1239 Size: 393 749 1638 597 787 268 1524 Fragments arranged by size: 1638 1524 787 749 597 393 268 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2251 2863 2251 Size: 612 5344 FseI GG_CCGG'CC Cuts at: 2203 2203 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2216 2248 2798 2830 4597 Size: 32 135 32 644 32 550 32 1767 Cuts at: 4597 4629 4671 4703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1767 644 550 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2074 2857 3300 3546 669 Size: 988 281 136 783 443 246 3079 Fragments arranged by size: 3079 988 783 443 281 246 136 HindIII A'AGCT_T Cuts at: 748 2448 3042 748 Size: 1700 594 3662 Fragments arranged by size: 3662 1700 594 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2633 2901 10 Size: 54 1425 343 801 268 3065 Fragments arranged by size: 3065 1425 801 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 3914 4273 4432 518 Size: 648 2748 359 159 2042 Fragments arranged by size: 2748 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2339 3372 4049 5197 5442 1606 Size: 733 1033 677 1148 245 2120 Fragments arranged by size: 2120 1148 1033 733 677 245 NaeI GCC'GGC Cuts at: 2201 2201 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 2529 513 Size: 552 1464 3940 Fragments arranged by size: 3940 1464 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2199 2199 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2342 1108 Size: 1234 4722 PfoI T'CCnGG_A Cuts at: 3210 3210 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3049 3081 4025 4057 5538 5570 3049 Size: 32 944 32 1481 32 3435 Fragments arranged by size: 3435 1481 944 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2952 1613 Size: 1339 4617 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2339 2339 Size: 5956 SacI G_AGCT'C Cuts at: 3210 3210 Size: 5956 SalI G'TCGA_C Cuts at: 1936 2072 3298 1936 Size: 136 1226 4594 Fragments arranged by size: 4594 1226 136 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 2231 4204 5916 2231 Size: 1973 1712 2271 Fragments arranged by size: 2271 1973 1712 SfcI C'TryA_G Cuts at: 826 1045 2250 3304 4439 5120 5311 826 Size: 219 1205 1054 1135 681 191 1471 Fragments arranged by size: 1471 1205 1135 1054 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3053 3283 1872 Size: 1181 230 4545 Fragments arranged by size: 4545 1181 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 4060 4961 5223 820 Size: 197 926 795 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3253 3880 5 Size: 47 3201 627 2081 Fragments arranged by size: 3201 2081 627 47 StuI AGG'CCT Cuts at: 1517 2603 1517 Size: 1086 4870 StyI C'CwwG_G Cuts at: 513 1065 2194 2529 2604 3395 513 Size: 552 1129 335 75 791 3074 Fragments arranged by size: 3074 1129 791 552 335 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3983 4000 4153 4338 4783 325 Size: 1255 2403 17 153 185 445 1498 Fragments arranged by size: 2403 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 4202 5914 Size: 274 80 33 51 1443 252 1973 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1973 1712 1443 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 2693 2861 1316 Size: 1377 168 4411 Fragments arranged by size: 4411 1377 168 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3182 3067 Size: 115 5841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3051 3281 1870 Size: 1181 230 4545 Fragments arranged by size: 4545 1181 230 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI