(Circular) (Six-base) MAPSORT of: pFN21AB5260.seq Check: 1392 from: 1 to: 5848 pFN21AB5260 5848 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 3911 5295 5563 1632 Size: 437 1842 1384 268 1917 Fragments arranged by size: 1917 1842 1384 437 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 2335 4577 2335 Size: 2242 3606 AleI CACnn'nnGTG Cuts at: 1166 3020 1166 Size: 1854 3994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 3591 5163 3591 Size: 1572 4276 ApaI G_GGCC'C Cuts at: 3036 3087 3036 Size: 51 5797 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 3157 3383 3535 5644 1493 Size: 1664 226 152 2109 1697 Fragments arranged by size: 2109 1697 1664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3006 3027 3173 1870 Size: 73 1063 21 146 4545 Fragments arranged by size: 4545 1063 146 73 21 BamHI G'GATC_C Cuts at: 2082 3178 3545 2082 Size: 1096 367 4385 Fragments arranged by size: 4385 1096 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2627 2897 2952 3169 4624 Size: 325 217 627 840 270 55 217 1455 Cuts at: 4624 618 Size: 1842 Fragments arranged by size: 1842 1455 840 627 325 270 217 217 55 BanII G_rGCy'C Cuts at: 1391 2823 3036 3087 3167 1391 Size: 1432 213 51 80 4072 Fragments arranged by size: 4072 1432 213 80 51 BbeI G_GCGC'C Cuts at: 2956 2956 Size: 5848 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BbvCI CC'TCA_GC Cuts at: 2723 2753 2723 Size: 30 5818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 2536 1548 Size: 988 4860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 4459 136 Size: 107 122 71 631 605 380 2407 1525 Fragments arranged by size: 2407 1525 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2900 3036 3087 3909 5065 1790 Size: 1110 136 51 822 1156 2573 Fragments arranged by size: 2573 1156 1110 822 136 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2801 2837 4508 1239 Size: 956 606 36 1671 2579 Fragments arranged by size: 2579 1671 956 606 36 Bpu10I CC'TnA_GC Cuts at: 2723 2753 2723 Size: 30 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 3023 3086 3973 4838 5136 2651 Size: 372 63 887 865 298 3363 Fragments arranged by size: 3363 887 865 372 298 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2767 4511 882 Size: 617 1268 1744 2219 Fragments arranged by size: 2219 1744 1268 617 BsaAI yAC'GTr Cuts at: 493 1204 2068 2560 2941 3639 493 Size: 711 864 492 381 698 2702 Fragments arranged by size: 2702 864 711 698 492 381 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2331 2953 3655 4037 Size: 53 83 186 936 798 622 702 382 Cuts at: 4037 4672 275 Size: 635 1451 Fragments arranged by size: 1451 936 798 702 635 622 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2559 2589 1178 Size: 30 165 30 1156 30 4437 Fragments arranged by size: 4437 1156 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2258 2310 2339 2455 2759 2948 1186 Size: 862 210 52 29 116 304 189 4086 Fragments arranged by size: 4086 862 304 210 189 116 52 29 BseYI C'CCAG_C Cuts at: 2483 3079 5051 5547 2483 Size: 596 1972 496 2784 Fragments arranged by size: 2784 1972 596 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 2090 1163 Size: 573 324 30 4921 Fragments arranged by size: 4921 573 324 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2646 2686 3298 4059 5087 1540 Size: 275 186 645 40 612 761 1028 2301 Fragments arranged by size: 2301 1028 761 645 612 275 186 40 BsiHKAI G_wGCw'C Cuts at: 2163 2415 3909 3994 5065 2163 Size: 252 1494 85 1071 2946 Fragments arranged by size: 2946 1494 1071 252 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2415 2823 2900 3036 3087 3167 Size: 399 373 252 408 77 136 51 80 Cuts at: 3167 3909 3994 5065 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 373 252 136 85 80 77 51 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2080 3203 844 Size: 1236 1123 3489 Fragments arranged by size: 3489 1236 1123 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2289 2697 2919 3000 3057 4492 4679 Size: 110 479 408 222 81 57 1435 187 Cuts at: 4679 1700 Size: 2869 Fragments arranged by size: 2869 1435 479 408 222 187 110 81 57 BsrGI T'GTAC_A Cuts at: 96 2316 96 Size: 2220 3628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 2575 1184 Size: 1391 4457 BstXI CCAn_nnnn'nTGG Cuts at: 3022 3022 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2082 3096 3178 3545 3930 3947 5399 5485 Size: 110 1014 82 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1014 385 367 346 110 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2802 513 Size: 552 939 798 3559 Fragments arranged by size: 3559 939 798 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2464 487 Size: 683 56 1238 3871 Fragments arranged by size: 3871 1238 683 56 BtsI GCAGTG_nn' Cuts at: 2987 3364 4158 4178 2987 Size: 377 794 20 4657 Fragments arranged by size: 4657 794 377 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2108 4855 809 Size: 507 792 2747 1802 Fragments arranged by size: 2747 1802 792 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 3012 3295 Size: 54 1425 50 275 18 558 624 283 Cuts at: 3295 4184 5813 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 624 558 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2420 3778 1353 Size: 1067 1358 3423 Fragments arranged by size: 3423 1358 1067 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2822 4435 4809 4955 387 Size: 1064 138 1233 1613 374 146 1280 Fragments arranged by size: 1613 1280 1233 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2195 2801 2837 3911 4508 5295 Size: 393 437 126 606 36 1074 597 787 Cuts at: 5295 5563 1239 Size: 268 1524 Fragments arranged by size: 1524 1074 787 606 597 437 393 268 126 36 EcoNI CCTnn'n_nnAGG Cuts at: 2344 2756 2344 Size: 412 5436 EcoO109I rG'GnC_Cy Cuts at: 2092 2960 3032 3033 3083 3084 2092 Size: 868 72 1 50 1 4856 Fragments arranged by size: 4856 868 72 50 1 1 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2618 2920 2956 4995 1678 Size: 940 302 36 2039 2531 Fragments arranged by size: 2531 2039 940 302 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2321 2353 4489 Size: 32 135 32 662 32 55 32 2136 Cuts at: 4489 4521 4563 4595 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2136 662 135 55 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KasI G'GCGC_C Cuts at: 2952 2952 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 4962 5146 1305 Size: 903 2754 184 2007 Fragments arranged by size: 2754 2007 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2390 10 Size: 54 1425 343 558 3468 Fragments arranged by size: 3468 1425 558 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2427 2708 3020 3806 4165 4324 518 Size: 648 1261 281 312 786 359 159 2042 Fragments arranged by size: 2042 1261 786 648 359 312 281 159 MspA1I CmG'CkG Cuts at: 1606 2074 3264 3941 5089 5334 1606 Size: 468 1190 677 1148 245 2120 Fragments arranged by size: 2120 1190 1148 677 468 245 NaeI GCC'GGC Cuts at: 2291 2291 Size: 5848 NarI GG'CG_CC Cuts at: 2953 2953 Size: 5848 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NgoMIV G'CCGG_C Cuts at: 2289 2289 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 3206 4751 1132 Size: 97 1977 1545 2229 Fragments arranged by size: 2229 1977 1545 97 PasI CC'CwG_GG Cuts at: 1348 2634 1348 Size: 1286 4562 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2960 2092 Size: 868 4980 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PspOMI G'GGCC_C Cuts at: 3032 3083 3032 Size: 51 5797 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2732 2764 2732 Size: 32 5816 PstI C_TGCA'G Cuts at: 830 2916 3200 830 Size: 2086 284 3478 Fragments arranged by size: 3478 2086 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SanDI GG'GwC_CC Cuts at: 2960 2960 Size: 5848 SapI GCTCTTCn'nnn_ Cuts at: 2420 2420 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 2912 3196 4331 5012 5203 826 Size: 219 1867 284 1135 681 191 1471 Fragments arranged by size: 1867 1471 1135 681 284 219 191 SfoI GGC'GCC Cuts at: 2954 2954 Size: 5848 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 2289 1700 Size: 589 5259 SmaI CCC'GGG Cuts at: 1872 3008 3029 3175 1872 Size: 1136 21 146 4545 Fragments arranged by size: 4545 1136 146 21 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 3038 3101 3952 4853 5115 Size: 197 926 723 372 63 851 901 262 Cuts at: 5115 820 Size: 1553 Fragments arranged by size: 1553 926 901 851 723 372 262 197 63 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 2391 3287 513 Size: 552 939 357 30 896 3074 Fragments arranged by size: 3074 939 896 552 357 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 3875 3892 4045 4230 4675 325 Size: 1255 983 1312 17 153 185 445 1498 Fragments arranged by size: 1498 1312 1255 983 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 4094 5806 Size: 274 80 33 51 1443 339 1778 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1778 1712 1443 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 5808 5840 2559 Size: 32 3217 32 2567 Fragments arranged by size: 3217 2567 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2834 2864 2383 Size: 451 30 5367 Fragments arranged by size: 5367 451 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3006 3027 3173 1870 Size: 1136 21 146 4545 Fragments arranged by size: 4545 1136 146 21 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SacII SexAI SfiI SrfI SwaI