Restriction map for pFN21AB5194
 (Circular) (Six-base) MAPSORT of: pFN21AB5194.seq  Check: 5181  from: 1  to: 5194



pFN21AB5194  5194 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3004     278 
   Size:         53      83     186     936    1468    2468

  Fragments arranged by size: 

                2468    1468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2537    1937 
   Size:        600    4594
 
Acc65I G'GTAC_C

Cuts at:   2515    2515 
   Size:       5194
 
AclI AA'CG_TT

Cuts at:   1563    3321    3694    1563 
   Size:       1758     373    3063

  Fragments arranged by size: 

                3063    1758     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2241    2463    3257    4641    4909    1632 
   Size:        609     222     794    1384     268    1917

  Fragments arranged by size: 

                1917    1384     794     609     268     222
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4997
 
AflIII A'CryG_T

Cuts at:   1203    4093    5070    1203 
   Size:       2890     977    1327

  Fragments arranged by size: 

                2890    1327     977
 
AgeI A'CCGG_T

Cuts at:   4025    4025 
   Size:       5194
 
AhdI GACnn_n'nnGTC

Cuts at:   3923    3923 
   Size:       5194
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5194
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5194
 
AlwNI CAG_nnn'CTG

Cuts at:   2937    4509    2937 
   Size:       1572    3622
 
ApaLI G'TGCA_C

Cuts at:   2188    3251    4407    2188 
   Size:       1063    1156    2975

  Fragments arranged by size: 

                2975    1156    1063
 
ApoI r'AATT_y

Cuts at:   1493    2276    2503    2729    2881    4990    1493 
   Size:        783     227     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     783     227     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3748     160 
   Size:        872    2716    1606

  Fragments arranged by size: 

                2716    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5194
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2519    1870 
   Size:         73     576    4545

  Fragments arranged by size: 

                4545     576      73
 
BamHI G'GATC_C

Cuts at:   2524    2891    2524 
   Size:        367    4827
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2515    3970     618 
   Size:        325     217     627     728    1455    1842

  Fragments arranged by size: 

                1842    1455     728     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2513    1391 
   Size:       1122    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4223
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3385    3419    1903 
   Size:         34      50      34    1364      34    3678

  Fragments arranged by size: 

                3678    1364      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3086    4296    3086 
   Size:       1210    3984
 
BclI T'GATC_A

Cuts at:   1548    2382    1548 
   Size:        834    4360
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3805     136 
   Size:        107     122      71     631     605    2133    1525

  Fragments arranged by size: 

                2133    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5189    5189 
   Size:       5194
 
BlpI GC'TnA_GC

Cuts at:   2611    2611 
   Size:       5194
 
Bme1580I G_kGCm'C

Cuts at:   1790    2192    3255    4411    1790 
   Size:        402    1063    1156    2573

  Fragments arranged by size: 

                2573    1156    1063     402
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5194
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2129    2925    3883 
   Size:        416      53      83     399      24     712     796     958

Cuts at:   3883     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     796     712     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3854    1239 
   Size:       2615    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3319    4184    4482    3319 
   Size:        865     298    4031

  Fragments arranged by size: 

                4031     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3857     882 
   Size:        617    2358    2219

  Fragments arranged by size: 

                2358    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2985     493 
   Size:        711    1781    2702

  Fragments arranged by size: 

                2702    1781     711
 
BsaBI GATnn'nnATC

Cuts at:   5188    5188 
   Size:       5194
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3001    3383    4018     275 
   Size:         53      83     186     936    1468     382     635    1451

  Fragments arranged by size: 

                1468    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3627    4025    4299    4446    4857    3627 
   Size:        398     274     147     411    3964

  Fragments arranged by size: 

                3964     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4969

  Fragments arranged by size: 

                4969     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2202    2205    2417    2462    1186 
   Size:       1016       3     212      45    3918

  Fragments arranged by size: 

                3918    1016     212      45       3
 
BseYI C'CCAG_C

Cuts at:   4397    4893    4397 
   Size:        496    4698
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4621
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2644    3405    4433    1540 
   Size:        275     186     643     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     643     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2192    3255    3340    4411    2192 
   Size:       1063      85    1071    2975

  Fragments arranged by size: 

                2975    1071    1063      85
 
BsmI GAATG_Cn'

Cuts at:   2161    2705    2798    2161 
   Size:        544      93    4557

  Fragments arranged by size: 

                4557     544      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2192    2513    3255    3340    4411    1391 
   Size:        399     402     321     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     402     399     321      85
 
BspEI T'CCGG_A

Cuts at:   4857    4857 
   Size:       5194
 
BspHI T'CATG_A

Cuts at:   3083    4813    3083 
   Size:       1730    3464
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2549     844 
   Size:       1705    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3081    1394 
   Size:        429    1258    3507

  Fragments arranged by size: 

                3507    1258     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3689    3863      65 
   Size:       1183    2441     174    1396

  Fragments arranged by size: 

                2441    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3838    4025    1700 
   Size:        110    2028     187    2869

  Fragments arranged by size: 

                2869    2028     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5194
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5194
 
BssSI C'ACGA_G

Cuts at:   3254    4266    3254 
   Size:       1012    4182
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5194
 
BstBI TT'CG_AA

Cuts at:   3998    3998 
   Size:       5194
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5194
 
BstYI r'GATC_y

Cuts at:   1972    2524    2891    3276    3293    4745    4831    4843    5189 
   Size:        552     367     385      17    1452      86      12     346

Cuts at:   5189    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     552     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2400    2400 
   Size:       5194
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4642
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4455

  Fragments arranged by size: 

                4455     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2710    3504    3524    2710 
   Size:        794      20    4380

  Fragments arranged by size: 

                4380     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5194
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5159
 
DraI TTT'AAA

Cuts at:   2498    2498 
   Size:       5194
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4201     809 
   Size:        507    2885    1802

  Fragments arranged by size: 

                2885    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2641    3530    5159 
   Size:         54    1425      50     275      18     811     889    1629

Cuts at:   5159       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     811     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2641    1537 
   Size:        275     829    4090

  Fragments arranged by size: 

                4090     829     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2439    2481    3124    1353 
   Size:       1086      42     643    3423

  Fragments arranged by size: 

                3423    1086     643      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3781    4155    4301     387 
   Size:       1064     138    2192     374     146    1280

  Fragments arranged by size: 

                2192    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2241    2463    3257    3854    4641    4909    1239 
   Size:        393     609     222     794     597     787     268    1524

  Fragments arranged by size: 

                1524     794     787     609     597     393     268     222
            
 
EcoRI G'AATT_C

Cuts at:   2276    2503    2881    2276 
   Size:        227     378    4589

  Fragments arranged by size: 

                4589     378     227
 
EcoRV GAT'ATC

Cuts at:   2257    2257 
   Size:       5194
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2318    2366    2318 
   Size:         48    5146
 
FspI TGC'GCA

Cuts at:   3700    3700 
   Size:       5194
 
HaeII r_GCGC'y

Cuts at:   1678    4341    1678 
   Size:       2663    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3835    3867    3909    3941    1373 
   Size:         32     135      32    2263      32      42      32    2626

  Fragments arranged by size: 

                2626    2263     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2290    2538    2784     669 
   Size:        988     281     352     248     246    3079

  Fragments arranged by size: 

                3079     988     352     281     248     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5194
 
HpaI GTT'AAC

Cuts at:   2784    2784 
   Size:       5194
 
KpnI G_GTAC'C

Cuts at:   2519    2519 
   Size:       5194
 
MfeI C'AATT_G

Cuts at:   2793    2793 
   Size:       5194
 
MluI A'CGCG_T

Cuts at:   5070    5070 
   Size:       5194
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2076    4308    4492    1305 
   Size:        771    2232     184    2007

  Fragments arranged by size: 

                2232    2007     771     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3372

  Fragments arranged by size: 

                3372    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2202    3152    3511    3670     518 
   Size:        648    1036     950     359     159    2042

  Fragments arranged by size: 

                2042    1036     950     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2610    3287    4435    4680    1606 
   Size:       1004     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1004     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4642
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5194
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5194
 
NotI GC'GGCC_GC

Cuts at:   2641    2641 
   Size:       5194
 
NspI r_CATG'y

Cuts at:   1132    1229    2364    2552    4097    1132 
   Size:         97    1135     188    1545    2229

  Fragments arranged by size: 

                2229    1545    1135     188      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5194
 
PciI A'CATG_T

Cuts at:   4093    4093 
   Size:       5194
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5194
 
PmeI GTTT'AAAC

Cuts at:   2498    2498 
   Size:       5194
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3263    3295    4776    4808    3263 
   Size:         32    1481      32    3649

  Fragments arranged by size: 

                3649    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5194
 
PsiI TTA'TAA

Cuts at:   2764    2764 
   Size:       5194
 
PstI C_TGCA'G

Cuts at:    830    2546     830 
   Size:       1716    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5194
 
SalI G'TCGA_C

Cuts at:   1936    2536    1936 
   Size:        600    4594
 
SbfI CC_TGCA'GG

Cuts at:   2546    2546 
   Size:       5194
 
ScaI AGT'ACT

Cuts at:   3442    5154    3442 
   Size:       1712    3482
 
SfcI C'TryA_G

Cuts at:    826    1045    2542    3677    4358    4549     826 
   Size:        219    1497    1135     681     191    1471

  Fragments arranged by size: 

                1497    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5194
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5194
 
SmaI CCC'GGG

Cuts at:   1872    2521    1872 
   Size:        649    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3298    4199    4461     820 
   Size:        197     926    1355     901     262    1553

  Fragments arranged by size: 

                1553    1355     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2985     493 
   Size:       2492    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5194
 
SphI G_CATG'C

Cuts at:   1132    2552    1132 
   Size:       1420    3774
 
SspI AAT'ATT

Cuts at:      5      52    2166    3118       5 
   Size:         47    2114     952    2081

  Fragments arranged by size: 

                2114    2081     952      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5194
 
StyI C'CwwG_G

Cuts at:    513    1065    2293    2633     513 
   Size:        552    1228     340    3074

  Fragments arranged by size: 

                3074    1228     552     340
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2207    3221    3238    3391    3576    4021     325 
   Size:       1255     627    1014      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1014     627     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3440    5152      96 
   Size:        274      80      33      51    1443    1463    1712     138

  Fragments arranged by size: 

                1712    1463    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5154    5186    5154 
   Size:         32    5162
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5194
 
XbaI T'CTAG_A

Cuts at:   2530    2530 
   Size:       5194
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5194
 
XmaI C'CCGG_G

Cuts at:   1870    2519    1870 
   Size:        649    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2313    3323    2313 
   Size:       1010    4184
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3002     276 
   Size:         53      83     186     936    1468    2468

  Fragments arranged by size: 

                2468    1468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   DraIII  EcoICRI    EcoNI EcoO109I     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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