################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeIII MamI MfeI MluI MroI MseI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AvaII 701 GGACC G^G[AT]CC AviII 749 TGCGCA TGC^GCA BbsI 343 GTCTTC GAAGAC(2/6) BbvCI 1024 GCTGAGG CCTCAGC(-5/-2) BpuAI 343 GTCTTC GAAGAC(2/6) BsaAI 577 TACGTG [CT]AC^GT[GA] BsaI 989 GAGACC GGTCTC(1/5) BseRI 490 CTCCTC GAGGAG(10/8) BsiEI 19 CGGCCG CG[GA][CT]^CG BsmBI 432 CGTCTC CGTCTC(1/5) BspMI 935 ACCTGC ACCTGC(4/8) BstXI 383 CCATCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 243 GGATCT [GA]^GATC[CT] EagI 19 CGGCCG C^GGCCG EclXI 19 CGGCCG C^GGCCG Eco52I 19 CGGCCG C^GGCCG FspI 749 TGCGCA TGC^GCA MflI 243 GGATCT [GA]^GATC[CT] NspI 784 GCATGT [GA]CATG^[CT] SapI 582 GCTCTTC GCTCTTC(1/4) SbfI 855 CCTGCAGG CCTGCA^GG SgrAI 26 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 855 CCTGCAGG CCTGCA^GG StuI 572 AGGCCT AGG^CCT TfiI 159 GAATC G^A[AT]TC Tsp509I 520 AATT ^AATT XcmI 505 CCAGCGCTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 243 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 655 GTAC GT^AC 850 GTAC AluI 71 AGCT AG^CT 1075 AGCT Alw44I 626 GTGCAC G^TGCAC 1148 GTGCAC AlwI 865 GATCC GGATC(4/5) 243 GGATC Aor51HI 507 AGCGCT AGC^GCT 1091 AGCGCT ApaI 421 GGGCCC GGGCC^C 792 GGGCCC ApaLI 626 GTGCAC G^TGCAC 1148 GTGCAC BfaI 154 CTAG C^TAG 163 CTAG BglI 678 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 775 GCCTTTGTGGC BlpI 369 GCTGAGC GC^T[ACGT]AGC 756 GCTCAGC BpmI 435 CTCCAG CTGGAG(16/14) 391 CTGGAG Bpu1102I 369 GCTGAGC GC^T[ACGT]AGC 756 GCTCAGC BsmAI 989 GAGAC GTCTC(1/5) 433 GTCTC BsrBI 292 CCGCTC CCGCTC(-3/-3) 559 CCGCTC BsrI 879 ACTGG ACTGG(1/-1) 437 CCAGT BstAPI 352 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 641 GCATCTACTGC BstF5I 276 CATCC GGATG(2/0) 609 CATCC CelII 369 GCTGAGC GC^T[ACGT]AGC 756 GCTCAGC EarI 583 CTCTTC CTCTTC(1/4) 799 CTCTTC Eco47III 507 AGCGCT AGC^GCT 1091 AGCGCT FokI 276 CATCC GGATG(9/13) 609 CATCC Ksp632I 583 CTCTTC CTCTTC(1/4) 799 CTCTTC KspI 75 CCGCGG CCGC^GG 467 CCGCGG MaeI 154 CTAG C^TAG 163 CTAG NcoI 407 CCATGG C^CATGG 884 CCATGG PleI 1167 GACTC GAGTC(4/5) 4 GAGTC PstI 856 CTGCAG CTGCA^G 1187 CTGCAG RsaI 655 GTAC GT^AC 850 GTAC SacII 75 CCGCGG CCGC^GG 467 CCGCGG SfaNI 608 GCATC GCATC(5/9) 641 GCATC SfcI 856 CTGCAG C^T[GA][CT]AG 1187 CTGCAG TaqI 709 TCGA T^CGA 818 TCGA TspRI 357 CACTG CA[GC]TG[ACGT][ACGT]^ 549 CACTG TthHB8I 709 TCGA T^CGA 818 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AspHI AvaI BalI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI SfoI SmaI StyI TseI XmaCI XmaI