(Circular) (Six-base) MAPSORT of: pFN21AB5174.seq Check: 1856 from: 1 to: 5899 pFN21AB5174 5899 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3067 3962 5346 5614 1632 Size: 1435 895 1384 268 1917 Fragments arranged by size: 1917 1435 1384 895 268 AfeI AGC'GCT Cuts at: 2514 3098 2514 Size: 584 5315 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 3642 5214 3642 Size: 1572 4327 ApaI G_GGCC'C Cuts at: 2430 2801 2430 Size: 371 5528 ApaLI G'TGCA_C Cuts at: 2631 3153 3956 5112 2631 Size: 522 803 1156 3418 Fragments arranged by size: 3418 1156 803 522 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2498 2693 2718 2998 3064 3224 1870 Size: 73 555 195 25 280 66 160 4545 Fragments arranged by size: 4545 555 280 195 160 73 66 25 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3162 3195 3162 Size: 33 5866 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2102 2261 2676 3220 Size: 325 217 627 242 73 159 415 544 Cuts at: 3220 4675 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 544 415 325 242 217 159 73 BanII G_rGCy'C Cuts at: 1391 2430 2505 2801 3218 1391 Size: 1039 75 296 417 4072 Fragments arranged by size: 4072 1039 417 296 75 BbeI G_GCGC'C Cuts at: 2033 2265 2680 2033 Size: 232 415 5252 Fragments arranged by size: 5252 415 232 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2341 928 Size: 971 442 4486 Fragments arranged by size: 4486 971 442 BbvCI CC'TCA_GC Cuts at: 3030 3030 Size: 5899 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2689 2786 4510 Size: 107 122 71 631 605 1017 97 1724 Cuts at: 4510 136 Size: 1525 Fragments arranged by size: 1724 1525 1017 631 605 122 107 97 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 2375 2762 3316 2375 Size: 387 554 4958 Fragments arranged by size: 4958 554 387 Bme1580I G_kGCm'C Cuts at: 1790 2430 2635 2801 2837 3157 3960 5116 1790 Size: 640 205 166 36 320 803 1156 2573 Fragments arranged by size: 2573 1156 803 640 320 205 166 36 BmgBI CAC'GTC Cuts at: 1318 2827 1318 Size: 1509 4390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 4588 442 Size: 416 53 83 399 24 2213 958 1753 Fragments arranged by size: 2213 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2425 4559 1239 Size: 1178 8 2134 2579 Fragments arranged by size: 2579 2134 1178 8 Bpu10I CC'TnA_GC Cuts at: 3030 3030 Size: 5899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4024 4889 5187 4024 Size: 865 298 4736 Fragments arranged by size: 4736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2988 4562 882 Size: 617 1489 1574 2219 Fragments arranged by size: 2219 1574 1489 617 BsaAI yAC'GTr Cuts at: 493 1204 2584 3690 493 Size: 711 1380 1106 2702 Fragments arranged by size: 2702 1380 1106 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2121 2191 2262 Size: 53 83 186 936 497 91 70 71 Cuts at: 2262 2346 2677 2977 3706 4088 4723 275 Size: 84 331 300 729 382 635 1451 Fragments arranged by size: 1451 936 729 635 497 382 331 300 186 91 84 83 71 70 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2564 1178 Size: 30 165 30 1131 30 4513 Fragments arranged by size: 4513 1131 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2486 1186 Size: 1300 4599 BseYI C'CCAG_C Cuts at: 2515 3009 3108 5102 5598 2515 Size: 494 99 1994 496 2816 Fragments arranged by size: 2816 1994 496 494 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2027 3349 4110 5138 1540 Size: 275 186 26 1322 761 1028 2301 Fragments arranged by size: 2301 1322 1028 761 275 186 26 BsiHKAI G_wGCw'C Cuts at: 2187 2589 2601 2635 2775 3157 3960 4045 5116 Size: 402 12 34 140 382 803 85 1071 Cuts at: 5116 2187 Size: 2970 Fragments arranged by size: 2970 1071 803 402 382 140 85 34 12 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 BsmBI CGTCTCn'nnnn_ Cuts at: 2443 2443 Size: 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2430 2505 2589 2601 2635 2775 Size: 399 397 243 75 84 12 34 140 Cuts at: 2775 2801 2837 3157 3218 3960 4045 5116 1391 Size: 26 36 320 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 320 243 140 85 84 75 61 36 34 26 12 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2949 3254 844 Size: 2105 305 3489 Fragments arranged by size: 3489 2105 305 BsrBI CCG'CTC Cuts at: 1394 1823 2299 2566 3786 1394 Size: 429 476 267 1220 3507 Fragments arranged by size: 3507 1220 476 429 267 BsrDI GCAATG_nn' Cuts at: 65 1248 4394 4568 65 Size: 1183 3146 174 1396 Fragments arranged by size: 3146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2026 2032 2069 2105 2683 3187 4543 Size: 110 216 6 37 36 578 504 1356 Cuts at: 4543 4730 1700 Size: 187 2869 Fragments arranged by size: 2869 1356 578 504 216 187 110 37 36 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 2087 2113 2417 2642 2840 1925 Size: 162 26 304 225 198 4984 Fragments arranged by size: 4984 304 225 198 162 26 BssSI C'ACGA_G Cuts at: 3959 4971 3959 Size: 1012 4887 BstAPI GCAn_nnn'nTGC Cuts at: 794 2363 2652 794 Size: 1569 289 4041 Fragments arranged by size: 4041 1569 289 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2395 Size: 5899 BstYI r'GATC_y Cuts at: 1972 2248 3229 3596 3981 3998 5450 5536 5548 Size: 276 981 367 385 17 1452 86 12 Cuts at: 5548 5894 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 981 385 367 346 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2080 2124 2412 2472 2889 513 Size: 552 939 76 44 288 60 417 3523 Fragments arranged by size: 3523 939 552 417 288 76 60 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2374 2477 3087 487 Size: 683 56 1148 103 610 3299 Fragments arranged by size: 3299 1148 683 610 103 56 BtsI GCAGTG_nn' Cuts at: 2361 3415 4209 4229 2361 Size: 1054 794 20 4031 Fragments arranged by size: 4031 1054 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2024 2385 2619 Size: 54 1425 50 275 18 194 361 234 Cuts at: 2619 2886 2892 3346 4235 5864 8 Size: 267 6 454 889 1629 43 Fragments arranged by size: 1629 1425 889 454 361 275 267 234 194 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2024 3346 1537 Size: 275 212 1322 4090 Fragments arranged by size: 4090 1322 275 212 EarI CTCTTCn'nnn_ Cuts at: 1353 2594 2810 3829 1353 Size: 1241 216 1019 3423 Fragments arranged by size: 3423 1241 1019 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2425 3067 3962 4559 5346 5614 Size: 393 785 8 642 895 597 787 268 Cuts at: 5614 1239 Size: 1524 Fragments arranged by size: 1524 895 787 785 642 597 393 268 8 EcoO109I rG'GnC_Cy Cuts at: 2426 2797 2798 3043 2426 Size: 371 1 245 5282 Fragments arranged by size: 5282 371 245 1 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FspI TGC'GCA Cuts at: 2756 4405 2756 Size: 1649 4250 HaeII r_GCGC'y Cuts at: 1678 2033 2265 2516 2680 3100 5046 1678 Size: 355 232 251 164 420 1946 2531 Fragments arranged by size: 2531 1946 420 355 251 232 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 3086 3118 4540 Size: 32 135 32 962 32 520 32 1422 Cuts at: 4540 4572 4614 4646 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 962 520 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KasI G'GCGC_C Cuts at: 2029 2261 2676 2029 Size: 232 415 5252 Fragments arranged by size: 5252 415 232 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 5013 5197 1305 Size: 921 2787 184 2007 Fragments arranged by size: 2787 2007 921 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 2888 2894 10 Size: 54 1425 343 555 501 6 3015 Fragments arranged by size: 3015 1425 555 501 343 54 6 MslI CAynn'nnrTG Cuts at: 518 1166 3857 4216 4375 518 Size: 648 2691 359 159 2042 Fragments arranged by size: 2691 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 2474 3054 3087 3132 3315 3992 5140 Size: 476 392 580 33 45 183 677 1148 Cuts at: 5140 5385 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 580 476 392 245 183 45 33 NaeI GCC'GGC Cuts at: 2028 2107 2685 2028 Size: 79 578 5242 Fragments arranged by size: 5242 578 79 NarI GG'CG_CC Cuts at: 2030 2262 2677 2030 Size: 232 415 5252 Fragments arranged by size: 5252 415 232 NcoI C'CATG_G Cuts at: 513 1065 2004 2412 2889 513 Size: 552 939 408 477 3523 Fragments arranged by size: 3523 939 552 477 408 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NgoMIV G'CCGG_C Cuts at: 2026 2105 2683 2026 Size: 79 578 5242 Fragments arranged by size: 5242 578 79 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 2793 3257 4802 1132 Size: 97 1564 464 1545 2229 Fragments arranged by size: 2229 1564 1545 464 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2426 2797 2426 Size: 371 5528 PstI C_TGCA'G Cuts at: 830 2865 3196 3251 830 Size: 2035 331 55 3478 Fragments arranged by size: 3478 2035 331 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 SacII CC_GC'GG Cuts at: 2083 2475 2083 Size: 392 5507 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SapI GCTCTTCn'nnn_ Cuts at: 2594 2594 Size: 5899 SbfI CC_TGCA'GG Cuts at: 2865 3251 2865 Size: 386 5513 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2861 3192 3247 4382 5063 5254 826 Size: 219 1816 331 55 1135 681 191 1471 Fragments arranged by size: 1816 1471 1135 681 331 219 191 55 SfoI GGC'GCC Cuts at: 2031 2263 2678 2031 Size: 232 415 5252 Fragments arranged by size: 5252 415 232 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 2032 1700 Size: 332 5567 SmaI CCC'GGG Cuts at: 1872 2695 2720 3066 3226 1872 Size: 823 25 346 160 4545 Fragments arranged by size: 4545 823 346 160 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 4003 4904 5166 820 Size: 197 926 2060 901 262 1553 Fragments arranged by size: 2060 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 2579 1517 Size: 1062 4837 StyI C'CwwG_G Cuts at: 513 1065 2004 2412 2574 2889 3338 513 Size: 552 939 408 162 315 449 3074 Fragments arranged by size: 3074 939 552 449 408 315 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3172 3926 3943 4096 4281 4726 325 Size: 1255 1592 754 17 153 185 445 1498 Fragments arranged by size: 1592 1498 1255 754 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2854 4145 5857 Size: 274 80 33 51 1443 877 1291 1712 Cuts at: 5857 96 Size: 138 Fragments arranged by size: 1712 1443 1291 877 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2517 2517 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 2693 2718 3064 3224 1870 Size: 823 25 346 160 4545 Fragments arranged by size: 4545 823 346 160 25 XmnI GAAnn'nnTTC Cuts at: 4028 4028 Size: 5899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI