(Linear) (Six-base) MAPSORT of: KIBB5174.seq Check: 8396 from: 1 to: 1194 KIBB5174.orf With 182 enzymes: SgfI * July 18, 2007 13:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1062 1194 Size: 1062 132 AfeI AGC'GCT Cuts at: 0 509 1093 1194 Size: 509 584 101 Fragments arranged by size: 584 509 101 ApaI G_GGCC'C Cuts at: 0 425 796 1194 Size: 425 371 398 Fragments arranged by size: 425 398 371 ApaLI G'TGCA_C Cuts at: 0 626 1148 1194 Size: 626 522 46 Fragments arranged by size: 626 522 46 AvaI C'yCGr_G Cuts at: 0 493 688 713 993 1059 1194 Size: 493 195 25 280 66 135 Fragments arranged by size: 493 280 195 135 66 25 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1157 1190 1194 Size: 1157 33 4 Fragments arranged by size: 1157 33 4 BanI G'GyrC_C Cuts at: 0 24 97 256 671 1194 Size: 24 73 159 415 523 Fragments arranged by size: 523 415 159 73 24 BanII G_rGCy'C Cuts at: 0 425 500 796 1194 Size: 425 75 296 398 Fragments arranged by size: 425 398 296 75 BbeI G_GCGC'C Cuts at: 0 28 260 675 1194 Size: 28 232 415 519 Fragments arranged by size: 519 415 232 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 336 1194 Size: 336 858 BbvCI CC'TCA_GC Cuts at: 0 1025 1194 Size: 1025 169 BglI GCCn_nnn'nGGC Cuts at: 0 684 781 1194 Size: 684 97 413 Fragments arranged by size: 684 413 97 BlpI GC'TnA_GC Cuts at: 0 370 757 1194 Size: 370 387 437 Fragments arranged by size: 437 387 370 Bme1580I G_kGCm'C Cuts at: 0 425 630 796 832 1152 1194 Size: 425 205 166 36 320 42 Fragments arranged by size: 425 320 205 166 42 36 BmgBI CAC'GTC Cuts at: 0 822 1194 Size: 822 372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 420 1194 Size: 412 8 774 Fragments arranged by size: 774 412 8 Bpu10I CC'TnA_GC Cuts at: 0 1025 1194 Size: 1025 169 BsaI GGTCTCn'nnnn_ Cuts at: 0 983 1194 Size: 983 211 BsaAI yAC'GTr Cuts at: 0 579 1194 Size: 579 615 BsaHI Gr'CG_yC Cuts at: 0 25 116 186 257 341 672 972 1194 Size: 25 91 70 71 84 331 300 222 Fragments arranged by size: 331 300 222 91 84 71 70 25 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 529 559 1194 Size: 529 30 635 Fragments arranged by size: 635 529 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 481 1194 Size: 481 713 BseYI C'CCAG_C Cuts at: 0 510 1004 1103 1194 Size: 510 494 99 91 Fragments arranged by size: 510 494 99 91 BsiEI CG_ry'CG Cuts at: 0 22 1194 Size: 22 1172 BsiHKAI G_wGCw'C Cuts at: 0 182 584 596 630 770 1152 1194 Size: 182 402 12 34 140 382 42 Fragments arranged by size: 402 382 182 140 42 34 12 BsmBI CGTCTCn'nnnn_ Cuts at: 0 438 1194 Size: 438 756 Bsp1286I G_dGCh'C Cuts at: 0 182 425 500 584 596 630 770 796 Size: 182 243 75 84 12 34 140 26 Cuts at: 796 832 1152 1194 Size: 36 320 42 Fragments arranged by size: 320 243 182 140 84 75 42 36 34 26 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 944 1194 Size: 944 250 BsrBI CCG'CTC Cuts at: 0 294 561 1194 Size: 294 267 633 Fragments arranged by size: 633 294 267 BsrFI r'CCGG_y Cuts at: 0 21 27 64 100 678 1182 1194 Size: 21 6 37 36 578 504 12 Fragments arranged by size: 578 504 37 36 21 12 6 BssHII G'CGCG_C Cuts at: 0 82 108 412 637 835 1194 Size: 82 26 304 225 198 359 Fragments arranged by size: 359 304 225 198 82 26 BstAPI GCAn_nnn'nTGC Cuts at: 0 358 647 1194 Size: 358 289 547 Fragments arranged by size: 547 358 289 BstXI CCAn_nnnn'nTGG Cuts at: 0 390 1194 Size: 390 804 BstYI r'GATC_y Cuts at: 0 243 1194 Size: 243 951 BtgI C'CryG_G Cuts at: 0 75 119 407 467 884 1194 Size: 75 44 288 60 417 310 Fragments arranged by size: 417 310 288 75 60 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 369 472 1082 1194 Size: 369 103 610 112 Fragments arranged by size: 610 369 112 103 BtsI GCAGTG_nn' Cuts at: 0 356 1194 Size: 356 838 EaeI y'GGCC_r Cuts at: 0 19 380 614 881 887 1194 Size: 19 361 234 267 6 307 Fragments arranged by size: 361 307 267 234 19 6 EagI C'GGCC_G Cuts at: 0 19 1194 Size: 19 1175 EarI CTCTTCn'nnn_ Cuts at: 0 589 805 1194 Size: 589 216 389 Fragments arranged by size: 589 389 216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 420 1062 1194 Size: 412 8 642 132 Fragments arranged by size: 642 412 132 8 EcoO109I rG'GnC_Cy Cuts at: 0 421 792 793 1038 1194 Size: 421 371 1 245 156 Fragments arranged by size: 421 371 245 156 1 FspI TGC'GCA Cuts at: 0 751 1194 Size: 751 443 HaeII r_GCGC'y Cuts at: 0 28 260 511 675 1095 1194 Size: 28 232 251 164 420 99 Fragments arranged by size: 420 251 232 164 99 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 529 561 1081 1113 1194 Size: 529 32 520 32 81 Fragments arranged by size: 529 520 81 32 32 KasI G'GCGC_C Cuts at: 0 24 256 671 1194 Size: 24 232 415 523 Fragments arranged by size: 523 415 232 24 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 221 1194 Size: 221 973 MscI TGG'CCA Cuts at: 0 382 883 889 1194 Size: 382 501 6 305 Fragments arranged by size: 501 382 305 6 MspA1I CmG'CkG Cuts at: 0 77 469 1049 1082 1127 1194 Size: 77 392 580 33 45 67 Fragments arranged by size: 580 392 77 67 45 33 NaeI GCC'GGC Cuts at: 0 23 102 680 1194 Size: 23 79 578 514 Fragments arranged by size: 578 514 79 23 NarI GG'CG_CC Cuts at: 0 25 257 672 1194 Size: 25 232 415 522 Fragments arranged by size: 522 415 232 25 NcoI C'CATG_G Cuts at: 0 407 884 1194 Size: 407 477 310 Fragments arranged by size: 477 407 310 NgoMIV G'CCGG_C Cuts at: 0 21 100 678 1194 Size: 21 79 578 516 Fragments arranged by size: 578 516 79 21 NspI r_CATG'y Cuts at: 0 788 1194 Size: 788 406 PspOMI G'GGCC_C Cuts at: 0 421 792 1194 Size: 421 371 402 Fragments arranged by size: 421 402 371 PstI C_TGCA'G Cuts at: 0 860 1191 1194 Size: 860 331 3 Fragments arranged by size: 860 331 3 SacII CC_GC'GG Cuts at: 0 78 470 1194 Size: 78 392 724 Fragments arranged by size: 724 392 78 SapI GCTCTTCn'nnn_ Cuts at: 0 589 1194 Size: 589 605 SbfI CC_TGCA'GG Cuts at: 0 860 1194 Size: 860 334 SfcI C'TryA_G Cuts at: 0 856 1187 1194 Size: 856 331 7 Fragments arranged by size: 856 331 7 SfoI GGC'GCC Cuts at: 0 26 258 673 1194 Size: 26 232 415 521 Fragments arranged by size: 521 415 232 26 SgrAI Cr'CCGG_yG Cuts at: 0 27 1194 Size: 27 1167 SmaI CCC'GGG Cuts at: 0 690 715 1061 1194 Size: 690 25 346 133 Fragments arranged by size: 690 346 133 25 StuI AGG'CCT Cuts at: 0 574 1194 Size: 574 620 StyI C'CwwG_G Cuts at: 0 407 569 884 1194 Size: 407 162 315 310 Fragments arranged by size: 407 315 310 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1167 1194 Size: 1167 27 TatI w'GTAC_w Cuts at: 0 849 1194 Size: 849 345 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 512 1194 Size: 512 682 XmaI C'CCGG_G Cuts at: 0 688 713 1059 1194 Size: 688 25 346 135 Fragments arranged by size: 688 346 135 25 Enzymes that do cut: AcuI AfeI ApaI ApaLI AvaI BaeI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoO109I FspI HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PspOMI PstI SacII SapI SbfI SfcI SfoI SgrAI SmaI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI