(Circular) (Six-base) MAPSORT of: pFN21AB5134.seq Check: 2218 from: 1 to: 7093 pFN21AB5134 7093 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2103 2131 2103 Size: 28 7065 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4903 278 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 4436 1937 Size: 883 1616 4594 Fragments arranged by size: 4594 1616 883 Acc65I G'GTAC_C Cuts at: 4414 4414 Size: 7093 AclI AA'CG_TT Cuts at: 1563 5220 5593 1563 Size: 3657 373 3063 Fragments arranged by size: 3657 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2538 3574 5156 6540 6808 1632 Size: 906 1036 1582 1384 268 1917 Fragments arranged by size: 1917 1582 1384 1036 906 268 AfeI AGC'GCT Cuts at: 2153 2153 Size: 7093 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6896 AflIII A'CryG_T Cuts at: 1203 2376 5992 6969 1203 Size: 1173 3616 977 1327 Fragments arranged by size: 3616 1327 1173 977 AgeI A'CCGG_T Cuts at: 5924 5924 Size: 7093 AhdI GACnn_n'nnGTC Cuts at: 5822 5822 Size: 7093 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7093 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7093 AlwNI CAG_nnn'CTG Cuts at: 3197 4836 6408 3197 Size: 1639 1572 3882 Fragments arranged by size: 3882 1639 1572 ApaLI G'TGCA_C Cuts at: 3059 5150 6306 3059 Size: 2091 1156 3846 Fragments arranged by size: 3846 2091 1156 ApoI r'AATT_y Cuts at: 1493 2435 3622 4402 4628 4780 6889 1493 Size: 942 1187 780 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 942 780 226 152 AseI AT'TA_AT Cuts at: 160 1032 2460 3303 5647 160 Size: 872 1428 843 2344 1606 Fragments arranged by size: 2344 1606 1428 872 843 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 AvaI C'yCGr_G Cuts at: 1870 1943 4418 1870 Size: 73 2475 4545 Fragments arranged by size: 4545 2475 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4085 4118 4085 Size: 33 7060 BamHI G'GATC_C Cuts at: 4423 4790 4423 Size: 367 6726 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2115 3200 3857 4414 Size: 325 217 627 304 24 1085 657 557 Cuts at: 4414 5869 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1085 657 627 557 325 304 217 24 BanII G_rGCy'C Cuts at: 1391 2371 3144 4128 4253 4274 4412 1391 Size: 980 773 984 125 21 138 4072 Fragments arranged by size: 4072 984 980 773 138 125 21 BbeI G_GCGC'C Cuts at: 3861 3861 Size: 7093 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4023 928 Size: 971 2124 3998 Fragments arranged by size: 3998 2124 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5284 5318 1903 Size: 34 3347 34 3678 Fragments arranged by size: 3678 3347 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2615 4985 6195 2615 Size: 2370 1210 3513 Fragments arranged by size: 3513 2370 1210 BclI T'GATC_A Cuts at: 1548 3502 3723 1548 Size: 1954 221 4918 Fragments arranged by size: 4918 1954 221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5704 136 Size: 107 122 71 631 605 4032 1525 Fragments arranged by size: 4032 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7088 7088 Size: 7093 BlpI GC'TnA_GC Cuts at: 4510 4510 Size: 7093 Bme1580I G_kGCm'C Cuts at: 1790 2094 2118 3063 3805 5154 6310 1790 Size: 304 24 945 742 1349 1156 2573 Fragments arranged by size: 2573 1349 1156 945 742 304 24 BmgBI CAC'GTC Cuts at: 1318 3793 1318 Size: 2475 4618 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2365 3416 4336 Size: 416 53 83 399 24 948 1051 920 Cuts at: 4336 4824 5782 442 Size: 488 958 1753 Fragments arranged by size: 1753 1051 958 948 920 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7093 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 4031 5753 1239 Size: 1202 1590 1722 2579 Fragments arranged by size: 2579 1722 1590 1202 Bpu10I CC'TnA_GC Cuts at: 2388 3848 4268 2388 Size: 1460 420 5213 Fragments arranged by size: 5213 1460 420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5218 6083 6381 5218 Size: 865 298 5930 Fragments arranged by size: 5930 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 5756 882 Size: 617 984 3273 2219 Fragments arranged by size: 3273 2219 984 617 BsaAI yAC'GTr Cuts at: 493 1204 2704 4884 493 Size: 711 1500 2180 2702 Fragments arranged by size: 2702 2180 1500 711 BsaBI GATnn'nnATC Cuts at: 3869 7087 3869 Size: 3218 3875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3858 4900 5282 5917 Size: 53 83 186 936 2325 1042 382 635 Cuts at: 5917 275 Size: 1451 Fragments arranged by size: 2325 1451 1042 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2473 5526 5924 6198 6345 6756 2473 Size: 3053 398 274 147 411 2810 Fragments arranged by size: 3053 2810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 4110 4140 4192 Size: 30 165 30 1011 30 1666 30 52 Cuts at: 4192 4222 1178 Size: 30 4049 Fragments arranged by size: 4049 1666 1011 165 52 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2708 4197 1186 Size: 1024 498 1489 4082 Fragments arranged by size: 4082 1489 1024 498 BseYI C'CCAG_C Cuts at: 2166 6296 6792 2166 Size: 4130 496 2467 Fragments arranged by size: 4130 2467 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2468 3567 1163 Size: 573 732 1099 4689 Fragments arranged by size: 4689 1099 732 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4543 5304 6332 1540 Size: 275 186 1270 1272 761 1028 2301 Fragments arranged by size: 2301 1272 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3063 3854 4274 5154 5239 6310 3063 Size: 791 420 880 85 1071 3846 Fragments arranged by size: 3846 1071 880 791 420 85 BsmI GAATG_Cn' Cuts at: 4604 4697 4604 Size: 93 7000 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2118 2371 3063 3144 3805 3854 Size: 399 304 24 253 692 81 661 49 Cuts at: 3854 4128 4253 4274 4412 5154 5239 6310 1391 Size: 274 125 21 138 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 661 399 304 274 253 138 125 85 81 49 24 21 BspEI T'CCGG_A Cuts at: 2473 6756 2473 Size: 4283 2810 BspHI T'CATG_A Cuts at: 4982 6712 4982 Size: 1730 5363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2131 4448 844 Size: 1259 28 2317 3489 Fragments arranged by size: 3489 2317 1259 28 BsrBI CCG'CTC Cuts at: 1394 1823 2083 4980 1394 Size: 429 260 2897 3507 Fragments arranged by size: 3507 2897 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 3091 5588 5762 65 Size: 1183 1843 2497 174 1396 Fragments arranged by size: 2497 1843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3842 5737 5924 1700 Size: 110 2032 1895 187 2869 Fragments arranged by size: 2869 2032 1895 187 110 BsrGI T'GTAC_A Cuts at: 96 2598 96 Size: 2502 4591 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7093 BssSI C'ACGA_G Cuts at: 2486 4171 5153 6165 2486 Size: 1685 982 1012 3414 Fragments arranged by size: 3414 1685 1012 982 BstAPI GCAn_nnn'nTGC Cuts at: 794 2055 794 Size: 1261 5832 BstBI TT'CG_AA Cuts at: 5897 5897 Size: 7093 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7093 BstXI CCAn_nnnn'nTGG Cuts at: 2350 2725 4147 2350 Size: 375 1422 5296 Fragments arranged by size: 5296 1422 375 BstYI r'GATC_y Cuts at: 1972 2884 4423 4790 5175 5192 6644 6730 6742 Size: 912 1539 367 385 17 1452 86 12 Cuts at: 6742 7088 1972 Size: 346 1977 Fragments arranged by size: 1977 1539 1452 912 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2821 2821 Size: 7093 BtgI C'CryG_G Cuts at: 513 1065 2110 3204 4200 513 Size: 552 1045 1094 996 3406 Fragments arranged by size: 3406 1094 1045 996 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3744 487 Size: 683 56 2518 3836 Fragments arranged by size: 3836 2518 683 56 BtsI GCAGTG_nn' Cuts at: 3803 4609 5403 5423 3803 Size: 806 794 20 5473 Fragments arranged by size: 5473 806 794 20 ClaI AT'CG_AT Cuts at: 1554 3267 1554 Size: 1713 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7058 DraI TTT'AAA Cuts at: 4397 4397 Size: 7093 DraIII CAC_nnn'GTG Cuts at: 2707 2707 Size: 7093 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6100 809 Size: 507 4784 1802 Fragments arranged by size: 4784 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2414 3426 3567 Size: 54 1425 50 275 18 584 1012 141 Cuts at: 3567 4130 4257 4284 4540 5429 7058 8 Size: 563 127 27 256 889 1629 43 Fragments arranged by size: 1629 1425 1012 889 584 563 275 256 141 127 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 4540 1537 Size: 275 2728 4090 Fragments arranged by size: 4090 2728 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2863 5023 1353 Size: 1002 508 2160 3423 Fragments arranged by size: 3423 2160 1002 508 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2757 5680 6054 6200 387 Size: 1064 138 1168 2923 374 146 1280 Fragments arranged by size: 2923 1280 1168 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4272 4272 Size: 7093 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 2538 3574 4031 5156 5753 6540 Size: 393 809 97 1036 457 1125 597 787 Cuts at: 6540 6808 1239 Size: 268 1524 Fragments arranged by size: 1524 1125 1036 809 787 597 457 393 268 97 EcoNI CCTnn'n_nnAGG Cuts at: 3480 3480 Size: 7093 EcoO109I rG'GnC_Cy Cuts at: 3436 4263 4331 3436 Size: 827 68 6198 Fragments arranged by size: 6198 827 68 EcoRI G'AATT_C Cuts at: 2435 4402 4780 2435 Size: 1967 378 4748 Fragments arranged by size: 4748 1967 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3718 3718 Size: 7093 FspI TGC'GCA Cuts at: 5599 5599 Size: 7093 HaeII r_GCGC'y Cuts at: 1678 2155 2589 3861 4139 6240 1678 Size: 477 434 1272 278 2101 2531 Fragments arranged by size: 2531 2101 1272 477 434 278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2306 2338 2370 Size: 32 135 32 662 32 40 32 32 Cuts at: 2370 2414 2446 4110 4142 5734 5766 5808 5840 Size: 44 32 1664 32 1592 32 42 32 Cuts at: 5840 1373 Size: 2626 Fragments arranged by size: 2626 1664 1592 662 135 44 42 40 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3448 4437 4683 669 Size: 988 281 1510 989 246 3079 Fragments arranged by size: 3079 1510 989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7093 HpaI GTT'AAC Cuts at: 4683 4683 Size: 7093 KasI G'GCGC_C Cuts at: 3857 3857 Size: 7093 KpnI G_GTAC'C Cuts at: 4418 4418 Size: 7093 MfeI C'AATT_G Cuts at: 3531 4692 3531 Size: 1161 5932 MluI A'CGCG_T Cuts at: 6969 6969 Size: 7093 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 2412 3546 6207 6391 1305 Size: 1026 81 1134 2661 184 2007 Fragments arranged by size: 2661 2007 1134 1026 184 81 MscI TGG'CCA Cuts at: 10 64 1489 1832 3428 10 Size: 54 1425 343 1596 3675 Fragments arranged by size: 3675 1596 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2306 2381 5051 5410 5569 518 Size: 648 898 242 75 2670 359 159 2042 Fragments arranged by size: 2670 2042 898 648 359 242 159 75 MspA1I CmG'CkG Cuts at: 1606 3194 4509 5186 6334 6579 1606 Size: 1588 1315 677 1148 245 2120 Fragments arranged by size: 2120 1588 1315 1148 677 245 NaeI GCC'GGC Cuts at: 3844 3844 Size: 7093 NarI GG'CG_CC Cuts at: 3858 3858 Size: 7093 NcoI C'CATG_G Cuts at: 513 1065 2110 513 Size: 552 1045 5496 Fragments arranged by size: 5496 1045 552 NdeI CA'TA_TG Cuts at: 387 2736 387 Size: 2349 4744 NgoMIV G'CCGG_C Cuts at: 3842 3842 Size: 7093 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7093 NotI GC'GGCC_GC Cuts at: 4540 4540 Size: 7093 NspI r_CATG'y Cuts at: 1132 1229 2053 3224 4114 4451 5996 1132 Size: 97 824 1171 890 337 1545 2229 Fragments arranged by size: 2229 1545 1171 890 824 337 97 PasI CC'CwG_GG Cuts at: 1348 2852 1348 Size: 1504 5589 PciI A'CATG_T Cuts at: 5992 5992 Size: 7093 PflMI CCAn_nnn'nTGG Cuts at: 1108 2894 3314 1108 Size: 1786 420 4887 Fragments arranged by size: 4887 1786 420 PfoI T'CCnGG_A Cuts at: 2451 2451 Size: 7093 PmeI GTTT'AAAC Cuts at: 4397 4397 Size: 7093 PmlI CAC'GTG Cuts at: 2704 2704 Size: 7093 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5162 5194 6675 6707 5162 Size: 32 1481 32 5548 Fragments arranged by size: 5548 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3436 4263 4331 3436 Size: 827 68 6198 Fragments arranged by size: 6198 827 68 PshAI GACnn'nnGTC Cuts at: 1613 2783 1613 Size: 1170 5923 PsiI TTA'TAA Cuts at: 4663 4663 Size: 7093 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2255 2287 2255 Size: 32 7061 PstI C_TGCA'G Cuts at: 830 4445 830 Size: 3615 3478 PvuI CG_AT'CG Cuts at: 2001 3271 2001 Size: 1270 5823 PvuII CAG'CTG Cuts at: 3194 3194 Size: 7093 SacI G_AGCT'C Cuts at: 4274 4274 Size: 7093 SalI G'TCGA_C Cuts at: 1936 4435 1936 Size: 2499 4594 SanDI GG'GwC_CC Cuts at: 4263 4263 Size: 7093 SbfI CC_TGCA'GG Cuts at: 4445 4445 Size: 7093 ScaI AGT'ACT Cuts at: 5341 7053 5341 Size: 1712 5381 SfcI C'TryA_G Cuts at: 826 1045 2147 3006 3031 4319 4441 5576 6257 Size: 219 1102 859 25 1288 122 1135 681 Cuts at: 6257 6448 826 Size: 191 1471 Fragments arranged by size: 1471 1288 1135 1102 859 681 219 191 122 25 SfoI GGC'GCC Cuts at: 3859 3859 Size: 7093 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7093 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7093 SmaI CCC'GGG Cuts at: 1872 4420 1872 Size: 2548 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5197 6098 6360 820 Size: 197 926 3254 901 262 1553 Fragments arranged by size: 3254 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4884 493 Size: 4391 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7093 SphI G_CATG'C Cuts at: 1132 2053 3224 4451 1132 Size: 921 1171 1227 3774 Fragments arranged by size: 3774 1227 1171 921 SspI AAT'ATT Cuts at: 5 52 2923 5017 5 Size: 47 2871 2094 2081 Fragments arranged by size: 2871 2094 2081 47 StuI AGG'CCT Cuts at: 1517 3827 1517 Size: 2310 4783 StyI C'CwwG_G Cuts at: 513 1065 2110 2688 2718 4532 513 Size: 552 1045 578 30 1814 3074 Fragments arranged by size: 3074 1814 1045 578 552 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 2795 5120 5137 5290 5475 5920 Size: 1255 1137 78 2325 17 153 185 445 Cuts at: 5920 325 Size: 1498 Fragments arranged by size: 2325 1498 1255 1137 445 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 2373 2598 Size: 274 80 33 51 1443 222 174 225 Cuts at: 2598 4107 5339 7051 96 Size: 1509 1232 1712 138 Fragments arranged by size: 1712 1509 1443 1232 274 225 222 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3289 3321 4192 4224 7053 7085 3289 Size: 32 871 32 2829 32 3297 Fragments arranged by size: 3297 2829 871 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7093 XbaI T'CTAG_A Cuts at: 4429 4429 Size: 7093 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 2416 3421 4055 2347 Size: 69 1005 634 5385 Fragments arranged by size: 5385 1005 634 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7093 XmaI C'CCGG_G Cuts at: 1870 4418 1870 Size: 2548 4545 XmnI GAAnn'nnTTC Cuts at: 2180 3609 5222 2180 Size: 1429 1613 4051 Fragments arranged by size: 4051 1613 1429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4901 276 Size: 53 83 186 936 3367 2468 Fragments arranged by size: 3367 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI Bsu36I EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI RsrII SacII SapI SexAI SfiI SrfI SwaI