(Circular) (Six-base) MAPSORT of: pFN21AB5121.seq Check: 7754 from: 1 to: 6127 pFN21AB5121 6127 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 3087 3470 1937 Size: 250 900 383 4594 Fragments arranged by size: 4594 900 383 250 Acc65I G'GTAC_C Cuts at: 2356 3271 3448 2356 Size: 915 177 5035 Fragments arranged by size: 5035 915 177 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4190 5574 5842 1632 Size: 2558 1384 268 1917 Fragments arranged by size: 2558 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2103 3424 5026 6003 1203 Size: 900 1321 1602 977 1327 Fragments arranged by size: 1602 1327 1321 977 900 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 3358 4856 3358 Size: 1498 4629 AleI CACnn'nnGTG Cuts at: 1166 2849 3161 1166 Size: 1683 312 4132 Fragments arranged by size: 4132 1683 312 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 2606 3870 5442 2606 Size: 1264 1572 3291 Fragments arranged by size: 3291 1572 1264 ApaI G_GGCC'C Cuts at: 2181 2181 Size: 6127 ApaLI G'TGCA_C Cuts at: 2076 4184 5340 2076 Size: 2108 1156 2863 Fragments arranged by size: 2863 2108 1156 ApoI r'AATT_y Cuts at: 1493 2573 3343 3436 3662 3814 5923 1493 Size: 1080 770 93 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 770 226 152 93 AscI GG'CGCG_CC Cuts at: 3250 3250 Size: 6127 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3452 1870 Size: 73 1509 4545 Fragments arranged by size: 4545 1509 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 3271 3448 4903 618 Size: 325 217 627 569 915 177 1455 1842 Fragments arranged by size: 1842 1455 915 627 569 325 217 177 BanII G_rGCy'C Cuts at: 1391 2181 3446 1391 Size: 790 1265 4072 Fragments arranged by size: 4072 1265 790 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5156 BbvCI CC'TCA_GC Cuts at: 3110 3110 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2703 4019 5229 2703 Size: 1316 1210 3601 Fragments arranged by size: 3601 1316 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BfrBI ATG'CAT Cuts at: 2816 2816 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2080 2181 4188 5344 1790 Size: 290 101 2007 1156 2573 Fragments arranged by size: 2573 2007 1156 290 101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3030 1056 Size: 1974 4153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 2990 3086 4787 1239 Size: 1316 435 96 1701 2579 Fragments arranged by size: 2579 1701 1316 435 96 Bpu10I CC'TnA_GC Cuts at: 3110 3227 3110 Size: 117 6010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4252 5117 5415 4252 Size: 865 298 4964 Fragments arranged by size: 4964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2233 2882 4790 882 Size: 617 734 649 1908 2219 Fragments arranged by size: 2219 1908 734 649 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 3918 493 Size: 711 909 1805 2702 Fragments arranged by size: 2702 1805 909 711 BsaBI GATnn'nnATC Cuts at: 2201 2928 6121 2201 Size: 727 3193 2207 Fragments arranged by size: 3193 2207 727 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3090 3934 4316 4951 Size: 53 83 186 936 1557 844 382 635 Cuts at: 4951 275 Size: 1451 Fragments arranged by size: 1557 1451 936 844 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3126 4560 4958 5232 5379 5790 3126 Size: 1434 398 274 147 411 3463 Fragments arranged by size: 3463 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 1178 Size: 30 165 30 810 30 5062 Fragments arranged by size: 5062 810 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2604 3098 1186 Size: 1418 494 4215 Fragments arranged by size: 4215 1418 494 BseYI C'CCAG_C Cuts at: 2096 3275 3306 5330 5826 2096 Size: 1179 31 2024 496 2397 Fragments arranged by size: 2397 2024 1179 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2512 1163 Size: 573 776 4778 Fragments arranged by size: 4778 776 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2165 3577 4338 5366 1540 Size: 275 186 164 1412 761 1028 2301 Fragments arranged by size: 2301 1412 1028 761 275 186 164 BsiHKAI G_wGCw'C Cuts at: 2080 3156 4188 4273 5344 2080 Size: 1076 1032 85 1071 2863 Fragments arranged by size: 2863 1076 1071 1032 85 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 BsmBI CGTCTCn'nnnn_ Cuts at: 3128 3128 Size: 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2181 3156 3446 4188 4273 5344 Size: 399 290 101 975 290 742 85 1071 Cuts at: 5344 1391 Size: 2174 Fragments arranged by size: 2174 1071 975 742 399 290 290 101 85 BspEI T'CCGG_A Cuts at: 3126 5790 3126 Size: 2664 3463 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3261 4771 4958 1700 Size: 110 1451 1510 187 2869 Fragments arranged by size: 2869 1510 1451 187 110 BsrGI T'GTAC_A Cuts at: 96 3421 96 Size: 3325 2802 BssHII G'CGCG_C Cuts at: 1925 3250 1925 Size: 1325 4802 BssSI C'ACGA_G Cuts at: 3157 4187 5199 3157 Size: 1030 1012 4085 Fragments arranged by size: 4085 1030 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 2500 4931 2500 Size: 2431 3696 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstYI r'GATC_y Cuts at: 1972 2494 2901 3321 3457 3824 4209 4226 5678 Size: 522 407 420 136 367 385 17 1452 Cuts at: 5678 5764 5776 6122 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 522 420 407 385 367 346 136 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2882 2882 Size: 6127 BtgI C'CryG_G Cuts at: 513 1065 2064 3094 513 Size: 552 999 1030 3546 Fragments arranged by size: 3546 1030 999 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 3643 4437 4457 3643 Size: 794 20 5313 Fragments arranged by size: 5313 794 20 ClaI AT'CG_AT Cuts at: 1554 2205 1554 Size: 651 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2162 3574 4463 Size: 54 1425 50 275 18 332 1412 889 Cuts at: 4463 6092 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1412 889 332 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2162 3574 1537 Size: 275 350 1412 4090 Fragments arranged by size: 4090 1412 350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4057 1353 Size: 2704 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2555 2990 3086 4190 4787 5574 5842 Size: 393 923 435 96 1104 597 787 268 Cuts at: 5842 1239 Size: 1524 Fragments arranged by size: 1524 1104 923 787 597 435 393 268 96 EcoO109I rG'GnC_Cy Cuts at: 2177 2178 3008 2177 Size: 1 830 5296 Fragments arranged by size: 5296 830 1 EcoRI G'AATT_C Cuts at: 3343 3436 3814 3343 Size: 93 378 5656 Fragments arranged by size: 5656 378 93 EcoRV GAT'ATC Cuts at: 2507 2507 Size: 6127 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2313 2313 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 3204 5274 1678 Size: 1526 2070 2531 Fragments arranged by size: 2531 2070 1526 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2372 2404 2451 Size: 32 135 32 641 32 127 32 47 Cuts at: 2451 2483 3344 3376 4768 4800 4842 4874 1373 Size: 32 861 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 861 641 135 127 47 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 2647 3088 3471 3717 669 Size: 988 281 250 459 441 383 246 3079 Fragments arranged by size: 3079 988 459 441 383 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 2360 3275 3452 2360 Size: 915 177 5035 Fragments arranged by size: 5035 915 177 MfeI C'AATT_G Cuts at: 2640 3726 2640 Size: 1086 5041 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2439 2849 3161 4085 4444 4603 Size: 648 1056 217 410 312 924 359 159 Cuts at: 4603 518 Size: 2042 Fragments arranged by size: 2042 1056 924 648 410 359 312 217 159 MspA1I CmG'CkG Cuts at: 1606 2480 3543 4220 5368 5613 1606 Size: 874 1063 677 1148 245 2120 Fragments arranged by size: 2120 1148 1063 874 677 245 NcoI C'CATG_G Cuts at: 513 1065 2064 513 Size: 552 999 4576 Fragments arranged by size: 4576 999 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 3026 1052 Size: 1974 4153 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2818 2818 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2107 2487 2761 3485 5030 1132 Size: 97 878 380 274 724 1545 2229 Fragments arranged by size: 2229 1545 878 724 380 274 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 2103 5026 2103 Size: 2923 3204 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 2177 2177 Size: 6127 PstI C_TGCA'G Cuts at: 830 2824 3479 830 Size: 1994 655 3478 Fragments arranged by size: 3478 1994 655 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2480 2480 Size: 6127 SalI G'TCGA_C Cuts at: 1936 2186 3086 3469 1936 Size: 250 900 383 4594 Fragments arranged by size: 4594 900 383 250 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2820 3475 4610 5291 5482 826 Size: 219 1775 655 1135 681 191 1471 Fragments arranged by size: 1775 1471 1135 681 655 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4231 5132 5394 820 Size: 197 926 2288 901 262 1553 Fragments arranged by size: 2288 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 3242 1517 Size: 1725 4402 StyI C'CwwG_G Cuts at: 513 1065 2064 3174 3566 513 Size: 552 999 1110 392 3074 Fragments arranged by size: 3074 1110 999 552 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 2891 4154 4171 4324 4509 4954 Size: 1255 1199 112 1263 17 153 185 445 Cuts at: 4954 325 Size: 1498 Fragments arranged by size: 1498 1263 1255 1199 445 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2014 3186 3421 Size: 274 80 33 51 1443 37 1172 235 Cuts at: 3421 4373 6085 96 Size: 952 1712 138 Fragments arranged by size: 1712 1443 1172 952 274 235 138 80 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 2225 1316 Size: 909 5218 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2221 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI