Restriction map for
pFN21AB5121
(Linear) (Six-base) MAPSORT of: KIBB5121.seq Check: 1298 from: 1 to: 1422
KIBB5121.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 182 1082 1422
Size: 182 900 340
Fragments arranged by size:
900 340 182
Acc65I G'GTAC_C
Cuts at: 0 351 1266 1422
Size: 351 915 156
Fragments arranged by size:
915 351 156
AflIII A'CryG_T
Cuts at: 0 98 1422
Size: 98 1324
AhdI GACnn_n'nnGTC
Cuts at: 0 1353 1422
Size: 1353 69
AleI CACnn'nnGTG
Cuts at: 0 844 1156 1422
Size: 844 312 266
Fragments arranged by size:
844 312 266
AlwNI CAG_nnn'CTG
Cuts at: 0 601 1422
Size: 601 821
ApaI G_GGCC'C
Cuts at: 0 176 1422
Size: 176 1246
ApaLI G'TGCA_C
Cuts at: 0 71 1422
Size: 71 1351
ApoI r'AATT_y
Cuts at: 0 568 1338 1422
Size: 568 770 84
Fragments arranged by size:
770 568 84
AscI GG'CGCG_CC
Cuts at: 0 1245 1422
Size: 1245 177
BanI G'GyrC_C
Cuts at: 0 351 1266 1422
Size: 351 915 156
Fragments arranged by size:
915 351 156
BanII G_rGCy'C
Cuts at: 0 176 1422
Size: 176 1246
BbvCI CC'TCA_GC
Cuts at: 0 1105 1422
Size: 1105 317
BciVI GTATCCnnnnn_n'
Cuts at: 0 698 1422
Size: 698 724
BfrBI ATG'CAT
Cuts at: 0 811 1422
Size: 811 611
Bme1580I G_kGCm'C
Cuts at: 0 75 176 1422
Size: 75 101 1246
Fragments arranged by size:
1246 101 75
BmtI G_CTAG'C
Cuts at: 0 1025 1422
Size: 1025 397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 985 1081 1422
Size: 550 435 96 341
Fragments arranged by size:
550 435 341 96
Bpu10I CC'TnA_GC
Cuts at: 0 1105 1222 1422
Size: 1105 117 200
Fragments arranged by size:
1105 200 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 228 877 1422
Size: 228 649 545
Fragments arranged by size:
649 545 228
BsaAI yAC'GTr
Cuts at: 0 108 1422
Size: 108 1314
BsaBI GATnn'nnATC
Cuts at: 0 196 923 1422
Size: 196 727 499
Fragments arranged by size:
727 499 196
BsaHI Gr'CG_yC
Cuts at: 0 1085 1422
Size: 1085 337
BsaWI w'CCGG_w
Cuts at: 0 1121 1422
Size: 1121 301
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 1422
Size: 208 30 1184
Fragments arranged by size:
1184 208 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 599 1093 1422
Size: 599 494 329
Fragments arranged by size:
599 494 329
BseYI C'CCAG_C
Cuts at: 0 91 1270 1301 1422
Size: 91 1179 31 121
Fragments arranged by size:
1179 121 91 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 1422
Size: 507 915
BsiEI CG_ry'CG
Cuts at: 0 160 1422
Size: 160 1262
BsiHKAI G_wGCw'C
Cuts at: 0 75 1151 1422
Size: 75 1076 271
Fragments arranged by size:
1076 271 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1123 1422
Size: 1123 299
Bsp1286I G_dGCh'C
Cuts at: 0 75 176 1151 1422
Size: 75 101 975 271
Fragments arranged by size:
975 271 101 75
BspEI T'CCGG_A
Cuts at: 0 1121 1422
Size: 1121 301
BsrFI r'CCGG_y
Cuts at: 0 1256 1422
Size: 1256 166
BsrGI T'GTAC_A
Cuts at: 0 1416 1422
Size: 1416 6
BssHII G'CGCG_C
Cuts at: 0 1245 1422
Size: 1245 177
BssSI C'ACGA_G
Cuts at: 0 1152 1422
Size: 1152 270
BstBI TT'CG_AA
Cuts at: 0 495 1422
Size: 495 927
BstYI r'GATC_y
Cuts at: 0 489 896 1316 1422
Size: 489 407 420 106
Fragments arranged by size:
489 420 407 106
Bsu36I CC'TnA_GG
Cuts at: 0 877 1422
Size: 877 545
BtgI C'CryG_G
Cuts at: 0 59 1089 1422
Size: 59 1030 333
Fragments arranged by size:
1030 333 59
ClaI AT'CG_AT
Cuts at: 0 200 1422
Size: 200 1222
EaeI y'GGCC_r
Cuts at: 0 157 1422
Size: 157 1265
EagI C'GGCC_G
Cuts at: 0 157 1422
Size: 157 1265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 985 1081 1422
Size: 550 435 96 341
Fragments arranged by size:
550 435 341 96
EcoO109I rG'GnC_Cy
Cuts at: 0 172 173 1003 1422
Size: 172 1 830 419
Fragments arranged by size:
830 419 172 1
EcoRI G'AATT_C
Cuts at: 0 1338 1422
Size: 1338 84
EcoRV GAT'ATC
Cuts at: 0 502 1422
Size: 502 920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 308 1422
Size: 308 1114
HaeII r_GCGC'y
Cuts at: 0 1199 1422
Size: 1199 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 367 399 446 478 1339 1371
Size: 208 32 127 32 47 32 861 32
Cuts at: 1371 1422
Size: 51
Fragments arranged by size:
861 208 127 51 47 32 32 32
32
HincII GTy'rAC
Cuts at: 0 183 642 1083 1422
Size: 183 459 441 339
Fragments arranged by size:
459 441 339 183
KpnI G_GTAC'C
Cuts at: 0 355 1270 1422
Size: 355 915 152
Fragments arranged by size:
915 355 152
MfeI C'AATT_G
Cuts at: 0 635 1422
Size: 635 787
MslI CAynn'nnrTG
Cuts at: 0 217 434 844 1156 1422
Size: 217 217 410 312 266
Fragments arranged by size:
410 312 266 217 217
MspA1I CmG'CkG
Cuts at: 0 475 1422
Size: 475 947
NcoI C'CATG_G
Cuts at: 0 59 1422
Size: 59 1363
NheI G'CTAG_C
Cuts at: 0 1021 1422
Size: 1021 401
NsiI A_TGCA'T
Cuts at: 0 813 1422
Size: 813 609
NspI r_CATG'y
Cuts at: 0 102 482 756 1422
Size: 102 380 274 666
Fragments arranged by size:
666 380 274 102
PciI A'CATG_T
Cuts at: 0 98 1422
Size: 98 1324
PspOMI G'GGCC_C
Cuts at: 0 172 1422
Size: 172 1250
PstI C_TGCA'G
Cuts at: 0 819 1422
Size: 819 603
PvuII CAG'CTG
Cuts at: 0 475 1422
Size: 475 947
SalI G'TCGA_C
Cuts at: 0 181 1081 1422
Size: 181 900 341
Fragments arranged by size:
900 341 181
SfcI C'TryA_G
Cuts at: 0 815 1422
Size: 815 607
StuI AGG'CCT
Cuts at: 0 1237 1422
Size: 1237 185
StyI C'CwwG_G
Cuts at: 0 59 1169 1422
Size: 59 1110 253
Fragments arranged by size:
1110 253 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 886 1422
Size: 774 112 536
Fragments arranged by size:
774 536 112
TatI w'GTAC_w
Cuts at: 0 9 1181 1416 1422
Size: 9 1172 235 6
Fragments arranged by size:
1172 235 9 6
Tth111I GACn'n_nGTC
Cuts at: 0 220 1422
Size: 220 1202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 1422
Size: 216 1206
Enzymes that do cut:
AccI Acc65I AflIII AhdI AleI AlwNI ApaI ApaLI
ApoI AscI BanI BanII BbvCI BciVI BfrBI Bme1580I
BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BsrFI BsrGI BssHII BssSI BstBI BstYI Bsu36I
BtgI ClaI EaeI EagI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII KpnI MfeI MslI MspA1I
NcoI NheI NsiI NspI PciI PspOMI PstI PvuII
SalI SfcI StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AlfI
AloI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbsI BcgI BclI BglI BglII BlpI BmgBI BmrI
BplI BpuEI BsiWI BsmI BspHI BspMI BsrBI BsrDI
BstAPI BstEII BstXI BstZ17I BtgZI BtsI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI FseI FspI
FspAI HindIII HpaI KasI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XhoI XmaI XmnI ZraI