Restriction map for
pFN21AB5115
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbrPI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI Bsu36I CelII CfoI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 3061 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1520 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3061 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BcgI 3096 GCATGGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 414 AGATCT A^GATCT
BsaI 2633 GAGACC GGTCTC(1/5)
BspDI 677 ATCGAT AT^CGAT
BsrBI 437 GAGCGG CCGCTC(-3/-3)
BsrFI 231 GCCGGT [GA]^CCGG[CT]
Bst1107I 823 GTATAC GTA^TAC
BstAPI 379 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 823 GTATAC GTA^TAC
Cfr10I 231 GCCGGT [GA]^CCGG[CT]
ClaI 677 ATCGAT AT^CGAT
DraIII 1091 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3061 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1043 CCCGC CCCGC(4/6)
HapII 232 CCGG C^CGG
HgaI 350 GCGTC GACGC(5/10)
HincII 1573 GTTGAC GT[CT]^[GA]AC
HindII 1573 GTTGAC GT[CT]^[GA]AC
HpaII 232 CCGG C^CGG
MaeII 2943 ACGT A^CGT
MfeI 1167 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 232 CCGG C^CGG
MunI 1167 CAATTG C^AATTG
NdeI 964 CATATG CA^TATG
PflMI 9 CCAGGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 614 CTGCAG CTGCA^G
PvuII 180 CAGCTG CAG^CTG
SacI 456 GAGCTC GAGCT^C
SapI 605 GCTCTTC GCTCTTC(1/4)
SphI 1905 GCATGC GCATG^C
SspI 1318 AATATT AAT^ATT
SwaI 993 ATTTAAAT ATTT^AAAT
Van91I 9 CCAGGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1520 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 739 GTCTAC GT^[AC][GT]AC
823 GTATAC
AlwNI 180 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
503 CAGAATCTG
ApaI 639 GGGCCC GGGCC^C
1870 GGGCCC
AspHI 386 GTGCTC G[AT]GC[AT]^C
456 GAGCTC
BanI 1921 GGTGCC G^G[CT][GA]CC
2029 GGTGCC
BbvCI 130 CCTCAGC CCTCAGC(-5/-2)
361 CCTCAGC
BfaI 959 CTAG C^TAG
3125 CTAG
BsiHKAI 386 GTGCTC G[AT]GC[AT]^C
456 GAGCTC
BspMI 2121 ACCTGC ACCTGC(4/8)
2163 GCAGGT
DraI 994 TTTAAA TTT^AAA
2799 TTTAAA
MaeI 959 CTAG C^TAG
3125 CTAG
MspA1I 180 CAGCTG C[AC]G^C[GT]G
657 CCGCTG
SexAI 194 ACCTGGT A^CC[AT]GGT
2718 ACCTGGT
SfcI 614 CTGCAG C^T[GA][CT]AG
1210 CTACAG
SmlI 933 CTCAAG C^T[CT][GA]AG
3278 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BanII BbsI
BbvI BclI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII