(Circular) (Six-base) MAPSORT of: pFN21AB5103.seq Check: 5895 from: 1 to: 6805 pFN21AB5103 6805 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4615 278 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3492 4148 1937 Size: 1555 656 4594 Fragments arranged by size: 4594 1555 656 Acc65I G'GTAC_C Cuts at: 4126 4126 Size: 6805 AclI AA'CG_TT Cuts at: 1563 4932 5305 1563 Size: 3369 373 3063 Fragments arranged by size: 3369 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2793 3804 4868 6252 6520 1632 Size: 398 763 1011 1064 1384 268 1917 Fragments arranged by size: 1917 1384 1064 1011 763 398 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6608 AflIII A'CryG_T Cuts at: 1203 3204 3531 5704 6681 1203 Size: 2001 327 2173 977 1327 Fragments arranged by size: 2173 2001 1327 977 327 AgeI A'CCGG_T Cuts at: 5636 5636 Size: 6805 AhdI GACnn_n'nnGTC Cuts at: 5534 5534 Size: 6805 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6805 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6805 AlwNI CAG_nnn'CTG Cuts at: 3814 3837 4548 6120 3814 Size: 23 711 1572 4499 Fragments arranged by size: 4499 1572 711 23 ApaLI G'TGCA_C Cuts at: 2340 4862 6018 2340 Size: 2522 1156 3127 Fragments arranged by size: 3127 2522 1156 ApoI r'AATT_y Cuts at: 1493 2444 2781 3396 3449 3553 4114 4340 4492 Size: 951 337 615 53 104 561 226 152 Cuts at: 4492 6601 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 951 615 561 337 226 152 104 53 AseI AT'TA_AT Cuts at: 160 1032 5359 160 Size: 872 4327 1606 Fragments arranged by size: 4327 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 AvaI C'yCGr_G Cuts at: 1870 1943 2257 4130 1870 Size: 73 314 1873 4545 Fragments arranged by size: 4545 1873 314 73 BamHI G'GATC_C Cuts at: 4135 4502 4135 Size: 367 6438 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2118 2268 2696 4126 5581 Size: 325 217 627 331 150 428 1430 1455 Cuts at: 5581 618 Size: 1842 Fragments arranged by size: 1842 1455 1430 627 428 331 325 217 150 BanII G_rGCy'C Cuts at: 1391 3291 4124 1391 Size: 1900 833 4072 Fragments arranged by size: 4072 1900 833 BbeI G_GCGC'C Cuts at: 2272 2272 Size: 6805 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5834 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3260 3294 4996 5030 1903 Size: 34 50 34 1239 34 1702 34 3678 Fragments arranged by size: 3678 1702 1239 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2992 4697 5907 2992 Size: 1705 1210 3890 Fragments arranged by size: 3890 1705 1210 BclI T'GATC_A Cuts at: 1548 4051 1548 Size: 2503 4302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3090 5416 136 Size: 107 122 71 631 605 1418 2326 1525 Fragments arranged by size: 2326 1525 1418 631 605 122 107 71 BglII A'GATC_T Cuts at: 6800 6800 Size: 6805 BlpI GC'TnA_GC Cuts at: 2858 4222 2858 Size: 1364 5441 Bme1580I G_kGCm'C Cuts at: 1790 2344 2701 4866 6022 1790 Size: 554 357 2165 1156 2573 Fragments arranged by size: 2573 2165 1156 554 357 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6805 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4536 5494 442 Size: 416 53 83 399 24 3119 958 1753 Fragments arranged by size: 3119 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6805 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2294 2345 3551 3814 5465 1239 Size: 962 93 51 1206 263 1651 2579 Fragments arranged by size: 2579 1651 1206 962 263 93 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4930 5795 6093 4930 Size: 865 298 5642 Fragments arranged by size: 5642 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2397 5468 882 Size: 617 898 3071 2219 Fragments arranged by size: 3071 2219 898 617 BsaAI yAC'GTr Cuts at: 493 1204 3532 4596 493 Size: 711 2328 1064 2702 Fragments arranged by size: 2702 2328 1064 711 BsaBI GATnn'nnATC Cuts at: 3321 6799 3321 Size: 3478 3327 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2269 4612 4994 5629 Size: 53 83 186 936 736 2343 382 635 Cuts at: 5629 275 Size: 1451 Fragments arranged by size: 2343 1451 936 736 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3647 5238 5636 5910 6057 6468 3647 Size: 1591 398 274 147 411 3984 Fragments arranged by size: 3984 1591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6580 Fragments arranged by size: 6580 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 2853 1186 Size: 939 728 5138 Fragments arranged by size: 5138 939 728 BseYI C'CCAG_C Cuts at: 2175 3888 6008 6504 2175 Size: 1713 2120 496 2476 Fragments arranged by size: 2476 2120 1713 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3789 3838 1163 Size: 573 2053 49 4130 Fragments arranged by size: 4130 2053 573 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4255 5016 6044 1540 Size: 275 186 2254 761 1028 2301 Fragments arranged by size: 2301 2254 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2344 3830 4866 4951 6022 2344 Size: 1486 1036 85 1071 3127 Fragments arranged by size: 3127 1486 1071 1036 85 BsmI GAATG_Cn' Cuts at: 2867 3788 4316 4409 2867 Size: 921 528 93 5263 Fragments arranged by size: 5263 921 528 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2732 2732 Size: 6805 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2344 2701 3291 3830 4124 4866 4951 Size: 399 554 357 590 539 294 742 85 Cuts at: 4951 6022 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 590 554 539 399 357 294 85 BspEI T'CCGG_A Cuts at: 6468 6468 Size: 6805 BspHI T'CATG_A Cuts at: 2947 4694 6424 2947 Size: 1747 1730 3328 Fragments arranged by size: 3328 1747 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3811 4031 4160 844 Size: 2967 220 129 3489 Fragments arranged by size: 3489 2967 220 129 BsrBI CCG'CTC Cuts at: 1394 1823 2100 4692 1394 Size: 429 277 2592 3507 Fragments arranged by size: 3507 2592 429 277 BsrDI GCAATG_nn' Cuts at: 65 1248 5300 5474 65 Size: 1183 4052 174 1396 Fragments arranged by size: 4052 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5449 5636 1700 Size: 110 3639 187 2869 Fragments arranged by size: 3639 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3099 96 Size: 3003 3802 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6805 BssSI C'ACGA_G Cuts at: 4865 5877 4865 Size: 1012 5793 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6805 BstBI TT'CG_AA Cuts at: 5609 5609 Size: 6805 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6805 BstYI r'GATC_y Cuts at: 1972 3754 4084 4135 4502 4887 4904 6356 6442 Size: 1782 330 51 367 385 17 1452 86 Cuts at: 6442 6454 6800 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1782 1452 385 367 346 330 86 51 17 12 BstZ17I GTA'TAC Cuts at: 3493 3493 Size: 6805 Bsu36I CC'TnA_GG Cuts at: 3777 3777 Size: 6805 BtgI C'CryG_G Cuts at: 513 1065 2043 3792 3861 513 Size: 552 978 1749 69 3457 Fragments arranged by size: 3457 1749 978 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6066 Fragments arranged by size: 6066 683 56 BtsI GCAGTG_nn' Cuts at: 3596 4321 5115 5135 3596 Size: 725 794 20 5266 Fragments arranged by size: 5266 794 725 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6805 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2912 2947 529 Size: 35 2348 35 4387 Fragments arranged by size: 4387 2348 35 35 DraI TTT'AAA Cuts at: 4109 4109 Size: 6805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5812 809 Size: 507 4496 1802 Fragments arranged by size: 4496 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 4252 5141 Size: 54 1425 50 275 18 210 2212 889 Cuts at: 5141 6770 8 Size: 1629 43 Fragments arranged by size: 2212 1629 1425 889 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4252 1537 Size: 275 2440 4090 Fragments arranged by size: 4090 2440 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2616 2735 4735 1353 Size: 1263 119 2000 3423 Fragments arranged by size: 3423 2000 1263 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5392 5766 5912 387 Size: 1064 138 3803 374 146 1280 Fragments arranged by size: 3803 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2201 2294 2345 2793 3551 3804 Size: 393 398 171 93 51 448 758 253 Cuts at: 3804 3814 4868 5465 6252 6520 1239 Size: 10 1054 597 787 268 1524 Fragments arranged by size: 1524 1054 787 758 597 448 398 393 268 253 171 93 51 10 EcoO109I rG'GnC_Cy Cuts at: 3627 3627 Size: 6805 EcoRI G'AATT_C Cuts at: 2444 3396 3449 4114 4492 2444 Size: 952 53 665 378 4757 Fragments arranged by size: 4757 952 665 378 53 EcoRV GAT'ATC Cuts at: 3986 3986 Size: 6805 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4004 4004 Size: 6805 FspI TGC'GCA Cuts at: 5311 5311 Size: 6805 HaeII r_GCGC'y Cuts at: 1678 2146 2272 5952 1678 Size: 468 126 3680 2531 Fragments arranged by size: 3680 2531 468 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3060 3092 3709 3741 4055 Size: 32 135 32 1488 32 617 32 314 Cuts at: 4055 4087 5446 5478 5520 5552 1373 Size: 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1488 1359 617 314 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4149 4395 669 Size: 988 281 2211 246 3079 Fragments arranged by size: 3079 2211 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6805 HpaI GTT'AAC Cuts at: 4395 4395 Size: 6805 KasI G'GCGC_C Cuts at: 2268 2268 Size: 6805 KpnI G_GTAC'C Cuts at: 4130 4130 Size: 6805 MfeI C'AATT_G Cuts at: 4404 4404 Size: 6805 MluI A'CGCG_T Cuts at: 6681 6681 Size: 6805 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5919 6103 1305 Size: 4614 184 2007 Fragments arranged by size: 4614 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4983 Fragments arranged by size: 4983 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 3942 4064 4763 5122 5281 518 Size: 648 2037 739 122 699 359 159 2042 Fragments arranged by size: 2042 2037 739 699 648 359 159 122 MspA1I CmG'CkG Cuts at: 1606 2179 4221 4898 6046 6291 1606 Size: 573 2042 677 1148 245 2120 Fragments arranged by size: 2120 2042 1148 677 573 245 NarI GG'CG_CC Cuts at: 2269 2269 Size: 6805 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6253 NdeI CA'TA_TG Cuts at: 387 387 Size: 6805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6805 NotI GC'GGCC_GC Cuts at: 4252 4252 Size: 6805 NspI r_CATG'y Cuts at: 1132 1229 3208 3933 4163 5708 1132 Size: 97 1979 725 230 1545 2229 Fragments arranged by size: 2229 1979 1545 725 230 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6805 PciI A'CATG_T Cuts at: 3204 5704 3204 Size: 2500 4305 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6805 PmeI GTTT'AAAC Cuts at: 4109 4109 Size: 6805 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4874 4906 6387 6419 4874 Size: 32 1481 32 5260 Fragments arranged by size: 5260 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3627 3627 Size: 6805 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6805 PsiI TTA'TAA Cuts at: 4375 4375 Size: 6805 PstI C_TGCA'G Cuts at: 830 3881 3908 4157 830 Size: 3051 27 249 3478 Fragments arranged by size: 3478 3051 249 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6805 PvuII CAG'CTG Cuts at: 2179 2179 Size: 6805 SalI G'TCGA_C Cuts at: 1936 4147 1936 Size: 2211 4594 SbfI CC_TGCA'GG Cuts at: 4157 4157 Size: 6805 ScaI AGT'ACT Cuts at: 2412 5053 6765 2412 Size: 2641 1712 2452 Fragments arranged by size: 2641 2452 1712 SfcI C'TryA_G Cuts at: 826 1045 3401 3631 3877 3904 4153 5288 5969 Size: 219 2356 230 246 27 249 1135 681 Cuts at: 5969 6160 826 Size: 191 1471 Fragments arranged by size: 2356 1471 1135 681 249 246 230 219 191 27 SfoI GGC'GCC Cuts at: 2270 2270 Size: 6805 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6805 SmaI CCC'GGG Cuts at: 1872 2259 4132 1872 Size: 387 1873 4545 Fragments arranged by size: 4545 1873 387 SmlI C'TyrA_G Cuts at: 820 1017 1943 4909 5810 6072 820 Size: 197 926 2966 901 262 1553 Fragments arranged by size: 2966 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4596 493 Size: 4103 2702 SpeI A'CTAG_T Cuts at: 152 3620 152 Size: 3468 3337 SphI G_CATG'C Cuts at: 1132 4163 1132 Size: 3031 3774 SspI AAT'ATT Cuts at: 5 52 4729 5 Size: 47 4677 2081 Fragments arranged by size: 4677 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6805 StyI C'CwwG_G Cuts at: 513 1065 3230 4244 513 Size: 552 2165 1014 3074 Fragments arranged by size: 3074 2165 1014 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3973 4832 4849 5002 5187 5632 325 Size: 1255 2393 859 17 153 185 445 1498 Fragments arranged by size: 2393 1498 1255 859 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2410 3099 5051 Size: 274 80 33 51 1443 433 689 1952 Cuts at: 5051 6763 96 Size: 1712 138 Fragments arranged by size: 1952 1712 1443 689 433 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3516 3548 6765 6797 3516 Size: 32 3217 32 3524 Fragments arranged by size: 3524 3217 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6805 XbaI T'CTAG_A Cuts at: 4141 4141 Size: 6805 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3728 3728 Size: 6805 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6805 XmaI C'CCGG_G Cuts at: 1870 2257 4130 1870 Size: 387 1873 4545 Fragments arranged by size: 4545 1873 387 XmnI GAAnn'nnTTC Cuts at: 4934 4934 Size: 6805 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4613 276 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI