(Circular) (Six-base) MAPSORT of: pFN21AB5063.seq Check: 6395 from: 1 to: 7030 pFN21AB5063 7030 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3432 4840 278 Size: 53 83 186 936 1896 1408 2468 Fragments arranged by size: 2468 1896 1408 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 4373 1937 Size: 1332 1104 4594 Fragments arranged by size: 4594 1332 1104 Acc65I G'GTAC_C Cuts at: 4351 4351 Size: 7030 AclI AA'CG_TT Cuts at: 1563 2425 5157 5530 1563 Size: 862 2732 373 3063 Fragments arranged by size: 3063 2732 862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 4128 4328 5093 6477 6745 1632 Size: 863 1633 200 765 1384 268 1917 Fragments arranged by size: 1917 1633 1384 863 765 268 200 AfeI AGC'GCT Cuts at: 2269 2269 Size: 7030 AflII C'TTAA_G Cuts at: 820 1017 2690 820 Size: 197 1673 5160 Fragments arranged by size: 5160 1673 197 AflIII A'CryG_T Cuts at: 1203 4165 5929 6906 1203 Size: 2962 1764 977 1327 Fragments arranged by size: 2962 1764 1327 977 AgeI A'CCGG_T Cuts at: 5861 5861 Size: 7030 AhdI GACnn_n'nnGTC Cuts at: 2023 5759 2023 Size: 3736 3294 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7030 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 2927 2959 2552 Size: 32 343 32 6623 Fragments arranged by size: 6623 343 32 32 AlwNI CAG_nnn'CTG Cuts at: 2134 2416 2656 2773 4013 4773 6345 2134 Size: 282 240 117 1240 760 1572 2819 Fragments arranged by size: 2819 1572 1240 760 282 240 117 ApaI G_GGCC'C Cuts at: 2540 3965 2540 Size: 1425 5605 ApaLI G'TGCA_C Cuts at: 5087 6243 5087 Size: 1156 5874 ApoI r'AATT_y Cuts at: 1493 3380 4339 4565 4717 6826 1493 Size: 1887 959 226 152 2109 1697 Fragments arranged by size: 2109 1887 1697 959 226 152 AseI AT'TA_AT Cuts at: 160 1032 5584 160 Size: 872 4552 1606 Fragments arranged by size: 4552 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7030 AvaI C'yCGr_G Cuts at: 1870 1943 2463 3615 4355 1870 Size: 73 520 1152 740 4545 Fragments arranged by size: 4545 1152 740 520 73 BamHI G'GATC_C Cuts at: 2163 4258 4360 4727 2163 Size: 2095 102 367 4466 Fragments arranged by size: 4466 2095 367 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2400 3778 4123 4351 5806 Size: 325 217 627 613 1378 345 228 1455 Cuts at: 5806 618 Size: 1842 Fragments arranged by size: 1842 1455 1378 627 613 345 325 228 217 BanII G_rGCy'C Cuts at: 1391 2259 2540 3051 3099 3965 4349 1391 Size: 868 281 511 48 866 384 4072 Fragments arranged by size: 4072 868 866 511 384 281 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3832 4320 928 Size: 971 1933 488 3638 Fragments arranged by size: 3638 1933 971 488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5221 5255 1903 Size: 34 3284 34 3678 Fragments arranged by size: 3678 3284 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2191 4922 6132 2191 Size: 2731 1210 3089 Fragments arranged by size: 3089 2731 1210 BclI T'GATC_A Cuts at: 1548 2496 1548 Size: 948 6082 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5641 136 Size: 107 122 71 631 605 337 3632 1525 Fragments arranged by size: 3632 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7025 7025 Size: 7030 BlpI GC'TnA_GC Cuts at: 2124 2264 4447 2124 Size: 140 2183 4707 Fragments arranged by size: 4707 2183 140 Bme1580I G_kGCm'C Cuts at: 1790 2540 3616 3783 3965 5091 6247 1790 Size: 750 1076 167 182 1126 1156 2573 Fragments arranged by size: 2573 1156 1126 1076 750 182 167 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7030 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3544 3960 4051 Size: 416 53 83 399 24 2127 416 91 Cuts at: 4051 4761 5719 442 Size: 710 958 1753 Fragments arranged by size: 2127 1753 958 710 416 416 399 91 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7030 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3113 3113 Size: 7030 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2795 3148 3488 3740 4007 4035 5690 Size: 1340 216 353 340 252 267 28 1655 Cuts at: 5690 1239 Size: 2579 Fragments arranged by size: 2579 1655 1340 353 340 267 252 216 28 Bpu10I CC'TnA_GC Cuts at: 2048 2048 Size: 7030 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2060 2297 5155 6020 6318 2060 Size: 237 2858 865 298 2772 Fragments arranged by size: 2858 2772 865 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3126 3794 5693 882 Size: 617 1627 668 1899 2219 Fragments arranged by size: 2219 1899 1627 668 617 BsaAI yAC'GTr Cuts at: 493 1204 2798 4168 4821 493 Size: 711 1594 1370 653 2702 Fragments arranged by size: 2702 1594 1370 711 653 BsaBI GATnn'nnATC Cuts at: 7024 7024 Size: 7030 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2206 3429 4837 5219 Size: 53 83 186 936 673 1223 1408 382 Cuts at: 5219 5854 275 Size: 635 1451 Fragments arranged by size: 1451 1408 1223 936 673 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2604 5463 5861 6135 6282 6693 2604 Size: 2859 398 274 147 411 2941 Fragments arranged by size: 2941 2859 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 3121 3151 3615 Size: 30 165 30 1149 30 539 30 464 Cuts at: 3615 3645 1178 Size: 30 4563 Fragments arranged by size: 4563 1149 539 464 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 3102 3107 3161 3881 1186 Size: 1201 715 5 54 720 4335 Fragments arranged by size: 4335 1201 720 715 54 5 BseYI C'CCAG_C Cuts at: 2259 2409 3790 6233 6729 2259 Size: 150 1381 2443 496 2560 Fragments arranged by size: 2560 2443 1381 496 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3355 3455 3464 1163 Size: 573 1619 100 9 4729 Fragments arranged by size: 4729 1619 573 100 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4480 5241 6269 1540 Size: 275 186 2479 761 1028 2301 Fragments arranged by size: 2479 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3051 3099 3354 5091 5176 6247 3051 Size: 48 255 1737 85 1071 3834 Fragments arranged by size: 3834 1737 1071 255 85 48 BsmI GAATG_Cn' Cuts at: 4541 4634 4541 Size: 93 6937 BsmBI CGTCTCn'nnnn_ Cuts at: 2063 2213 2063 Size: 150 6880 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2540 3051 3099 3354 3616 3783 Size: 399 469 281 511 48 255 262 167 Cuts at: 3783 3965 4349 5091 5176 6247 1391 Size: 182 384 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 511 469 399 384 281 262 255 182 167 85 48 BspEI T'CCGG_A Cuts at: 6693 6693 Size: 7030 BspHI T'CATG_A Cuts at: 4919 6649 4919 Size: 1730 5300 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2509 2782 3477 4004 4385 844 Size: 1665 273 695 527 381 3489 Fragments arranged by size: 3489 1665 695 527 381 273 BsrBI CCG'CTC Cuts at: 1394 1823 2329 4917 1394 Size: 429 506 2588 3507 Fragments arranged by size: 3507 2588 506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3177 3424 5525 5699 65 Size: 1183 1929 247 2101 174 1396 Fragments arranged by size: 2101 1929 1396 1183 247 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2403 5674 5861 1700 Size: 110 593 3271 187 2869 Fragments arranged by size: 3271 2869 593 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7030 BssHII G'CGCG_C Cuts at: 1925 2094 1925 Size: 169 6861 BssSI C'ACGA_G Cuts at: 3702 5090 6102 3702 Size: 1388 1012 4630 Fragments arranged by size: 4630 1388 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3521 794 Size: 2727 4303 BstBI TT'CG_AA Cuts at: 5834 5834 Size: 7030 BstEII G'GTnAC_C Cuts at: 1184 3991 1184 Size: 2807 4223 BstXI CCAn_nnnn'nTGG Cuts at: 2705 3766 2705 Size: 1061 5969 BstYI r'GATC_y Cuts at: 1972 2163 2244 3498 4111 4258 4360 4727 5112 Size: 191 81 1254 613 147 102 367 385 Cuts at: 5112 5129 6581 6667 6679 7025 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1254 613 385 367 346 191 147 102 86 81 17 12 BstZ17I GTA'TAC Cuts at: 3270 3270 Size: 7030 Bsu36I CC'TnA_GG Cuts at: 3591 3917 3591 Size: 326 6704 BtgI C'CryG_G Cuts at: 513 1065 2004 3141 3545 3708 4254 513 Size: 552 939 1137 404 163 546 3289 Fragments arranged by size: 3289 1137 939 552 546 404 163 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6291 Fragments arranged by size: 6291 683 56 BtsI GCAGTG_nn' Cuts at: 3152 3328 3452 4546 5340 5360 3152 Size: 176 124 1094 794 20 4822 Fragments arranged by size: 4822 1094 794 176 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7030 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2604 2639 529 Size: 35 2040 35 4920 Fragments arranged by size: 4920 2040 35 35 DraI TTT'AAA Cuts at: 4334 4334 Size: 7030 DraIII CAC_nnn'GTG Cuts at: 4045 4171 4045 Size: 126 6904 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2519 6037 809 Size: 507 1203 3518 1802 Fragments arranged by size: 3518 1802 1203 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2952 3457 4477 Size: 54 1425 50 275 18 1122 505 1020 Cuts at: 4477 5366 6995 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1122 1020 889 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4477 1537 Size: 275 2665 4090 Fragments arranged by size: 4090 2665 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 3403 4960 1353 Size: 683 1367 1557 3423 Fragments arranged by size: 3423 1557 1367 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5617 5991 6137 387 Size: 1064 138 4028 374 146 1280 Fragments arranged by size: 4028 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3049 3097 3049 Size: 48 6982 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 2579 2795 3148 3488 3740 4007 Size: 393 863 84 216 353 340 252 267 Cuts at: 4007 4035 4128 4328 5093 5690 6477 6745 1239 Size: 28 93 200 765 597 787 268 1524 Fragments arranged by size: 1524 863 787 765 597 393 353 340 268 267 252 216 200 93 84 28 EcoO109I rG'GnC_Cy Cuts at: 2521 2537 2686 3961 3962 2521 Size: 16 149 1275 1 5589 Fragments arranged by size: 5589 1275 149 16 1 EcoRI G'AATT_C Cuts at: 3380 4339 4717 3380 Size: 959 378 5693 Fragments arranged by size: 5693 959 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4303 4303 Size: 7030 FspI TGC'GCA Cuts at: 3176 5536 3176 Size: 2360 4670 HaeII r_GCGC'y Cuts at: 1678 2271 3892 6177 1678 Size: 593 1621 2285 2531 Fragments arranged by size: 2531 2285 1621 593 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2644 2676 3121 Size: 32 135 32 437 32 603 32 445 Cuts at: 3121 3153 5671 5703 5745 5777 1373 Size: 32 2518 32 42 32 2626 Fragments arranged by size: 2626 2518 603 445 437 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4374 4620 669 Size: 988 281 2436 246 3079 Fragments arranged by size: 3079 2436 988 281 246 HindIII A'AGCT_T Cuts at: 748 4280 748 Size: 3532 3498 HpaI GTT'AAC Cuts at: 4620 4620 Size: 7030 KpnI G_GTAC'C Cuts at: 4355 4355 Size: 7030 MfeI C'AATT_G Cuts at: 4629 4629 Size: 7030 MluI A'CGCG_T Cuts at: 6906 6906 Size: 7030 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2526 6144 6328 1305 Size: 1221 3618 184 2007 Fragments arranged by size: 3618 2007 1221 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5208 Fragments arranged by size: 5208 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 3776 4988 5347 5506 518 Size: 648 2547 63 1212 359 159 2042 Fragments arranged by size: 2547 2042 1212 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 3794 4446 5123 6271 6516 1606 Size: 2188 652 677 1148 245 2120 Fragments arranged by size: 2188 2120 1148 677 652 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3141 3708 513 Size: 552 939 1137 567 3835 Fragments arranged by size: 3835 1137 939 567 552 NdeI CA'TA_TG Cuts at: 387 3004 387 Size: 2617 4413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7030 NotI GC'GGCC_GC Cuts at: 4477 4477 Size: 7030 NspI r_CATG'y Cuts at: 1132 1229 2518 2846 3953 4388 5933 1132 Size: 97 1289 328 1107 435 1545 2229 Fragments arranged by size: 2229 1545 1289 1107 435 328 97 PasI CC'CwG_GG Cuts at: 1348 2134 1348 Size: 786 6244 PciI A'CATG_T Cuts at: 5929 5929 Size: 7030 PflMI CCAn_nnn'nTGG Cuts at: 1108 2416 2533 3910 1108 Size: 1308 117 1377 4228 Fragments arranged by size: 4228 1377 1308 117 PmeI GTTT'AAAC Cuts at: 4334 4334 Size: 7030 PmlI CAC'GTG Cuts at: 4168 4168 Size: 7030 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2552 2584 5099 5131 6612 6644 2552 Size: 32 2515 32 1481 32 2938 Fragments arranged by size: 2938 2515 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2521 2521 Size: 7030 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7030 PsiI TTA'TAA Cuts at: 4600 4600 Size: 7030 PspOMI G'GGCC_C Cuts at: 2536 3961 2536 Size: 1425 5605 PstI C_TGCA'G Cuts at: 830 3948 4001 4382 830 Size: 3118 53 381 3478 Fragments arranged by size: 3478 3118 381 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7030 PvuII CAG'CTG Cuts at: 3794 3794 Size: 7030 SacI G_AGCT'C Cuts at: 3051 3099 3051 Size: 48 6982 SalI G'TCGA_C Cuts at: 1936 4372 1936 Size: 2436 4594 SapI GCTCTTCn'nnn_ Cuts at: 2036 3403 2036 Size: 1367 5663 SbfI CC_TGCA'GG Cuts at: 4382 4382 Size: 7030 ScaI AGT'ACT Cuts at: 5278 6990 5278 Size: 1712 5318 SfcI C'TryA_G Cuts at: 826 1045 3249 3944 3997 4378 5513 6194 6385 Size: 219 2204 695 53 381 1135 681 191 Cuts at: 6385 826 Size: 1471 Fragments arranged by size: 2204 1471 1135 695 681 381 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7030 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7030 SmaI CCC'GGG Cuts at: 1872 3617 4357 1872 Size: 1745 740 4545 Fragments arranged by size: 4545 1745 740 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 2276 2690 5134 6035 6297 Size: 197 926 132 201 414 2444 901 262 Cuts at: 6297 820 Size: 1553 Fragments arranged by size: 2444 1553 926 901 414 262 201 197 132 SnaBI TAC'GTA Cuts at: 493 2798 4821 493 Size: 2305 2023 2702 Fragments arranged by size: 2702 2305 2023 SpeI A'CTAG_T Cuts at: 152 152 Size: 7030 SphI G_CATG'C Cuts at: 1132 4388 1132 Size: 3256 3774 SspI AAT'ATT Cuts at: 5 52 4954 5 Size: 47 4902 2081 Fragments arranged by size: 4902 2081 47 StuI AGG'CCT Cuts at: 1517 4031 1517 Size: 2514 4516 StyI C'CwwG_G Cuts at: 513 1065 2004 2298 2877 3141 3375 3708 3765 Size: 552 939 294 579 264 234 333 57 Cuts at: 3765 4469 513 Size: 704 3074 Fragments arranged by size: 3074 939 704 579 552 333 294 264 234 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2333 3847 4033 5057 5074 5227 5412 Size: 1255 753 1514 186 1024 17 153 185 Cuts at: 5412 5857 325 Size: 445 1498 Fragments arranged by size: 1514 1498 1255 1024 753 445 186 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5276 6988 96 Size: 274 80 33 51 1443 3299 1712 138 Fragments arranged by size: 3299 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3284 3316 3615 3647 3733 3765 6990 7022 3284 Size: 32 299 32 86 32 3225 32 3292 Fragments arranged by size: 3292 3225 299 86 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2656 1316 Size: 1340 5690 XbaI T'CTAG_A Cuts at: 2014 3661 4366 2014 Size: 1647 705 4678 Fragments arranged by size: 4678 1647 705 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2314 2962 3715 2314 Size: 648 753 5629 Fragments arranged by size: 5629 753 648 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7030 XmaI C'CCGG_G Cuts at: 1870 3615 4355 1870 Size: 1745 740 4545 Fragments arranged by size: 4545 1745 740 XmnI GAAnn'nnTTC Cuts at: 2567 5159 2567 Size: 2592 4438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3430 4838 276 Size: 53 83 186 936 1896 1408 2468 Fragments arranged by size: 2468 1896 1408 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI