(Circular) (Six-base) MAPSORT of: pFN21AB4969.seq Check: 5814 from: 1 to: 11377 pFN21AB4969 11377 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7500 7500 Size: 11377 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9187 278 Size: 53 83 186 936 7651 2468 Fragments arranged by size: 7651 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 4549 5059 8720 1937 Size: 262 2350 510 3661 4594 Fragments arranged by size: 4594 3661 2350 510 262 Acc65I G'GTAC_C Cuts at: 3675 6714 8698 3675 Size: 3039 1984 6354 Fragments arranged by size: 6354 3039 1984 AclI AA'CG_TT Cuts at: 1563 9504 9877 1563 Size: 7941 373 3063 Fragments arranged by size: 7941 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 4170 4521 5084 5412 5854 5989 6783 Size: 669 1869 351 563 328 442 135 794 Cuts at: 6783 6975 7764 7979 9440 10824 11092 1632 Size: 192 789 215 1461 1384 268 1917 Fragments arranged by size: 1917 1869 1461 1384 794 789 669 563 442 351 328 268 215 192 135 AflII C'TTAA_G Cuts at: 820 1017 3011 5195 820 Size: 197 1994 2184 7002 Fragments arranged by size: 7002 2184 1994 197 AflIII A'CryG_T Cuts at: 1203 8326 8563 10276 11253 1203 Size: 7123 237 1713 977 1327 Fragments arranged by size: 7123 1713 1327 977 237 AgeI A'CCGG_T Cuts at: 10208 10208 Size: 11377 AhdI GACnn_n'nnGTC Cuts at: 10106 10106 Size: 11377 AleI CACnn'nnGTG Cuts at: 1166 8475 1166 Size: 7309 4068 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 11377 AlwNI CAG_nnn'CTG Cuts at: 2830 7722 9120 10692 2830 Size: 4892 1398 1572 3515 Fragments arranged by size: 4892 3515 1572 1398 ApaI G_GGCC'C Cuts at: 2571 2909 2571 Size: 338 11039 ApaLI G'TGCA_C Cuts at: 9434 10590 9434 Size: 1156 10221 ApoI r'AATT_y Cuts at: 1493 3864 4003 4037 4292 4571 5311 6201 6434 Size: 2371 139 34 255 279 740 890 233 Cuts at: 6434 6521 6788 6930 6988 7307 7871 8686 8912 Size: 87 267 142 58 319 564 815 226 Cuts at: 8912 9064 11173 1493 Size: 152 2109 1697 Fragments arranged by size: 2371 2109 1697 890 815 740 564 319 279 267 255 233 226 152 142 139 87 58 34 AseI AT'TA_AT Cuts at: 160 1032 7428 9931 160 Size: 872 6396 2503 1606 Fragments arranged by size: 6396 2503 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11377 AvaI C'yCGr_G Cuts at: 1870 1943 2554 2788 6903 8142 8702 1870 Size: 73 611 234 4115 1239 560 4545 Fragments arranged by size: 4545 4115 1239 611 560 234 73 BamHI G'GATC_C Cuts at: 4516 7890 7929 8707 9074 4516 Size: 3374 39 778 367 6819 Fragments arranged by size: 6819 3374 778 367 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 3675 6714 7943 8698 Size: 325 217 627 261 1627 3039 1229 755 Cuts at: 8698 10153 618 Size: 1455 1842 Fragments arranged by size: 3039 1842 1627 1455 1229 755 627 325 261 217 BanII G_rGCy'C Cuts at: 1391 2151 2571 2789 2909 3280 5463 7806 8696 Size: 760 420 218 120 371 2183 2343 890 Cuts at: 8696 1391 Size: 4072 Fragments arranged by size: 4072 2343 2183 890 760 420 371 218 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2624 4080 4509 4902 5197 6822 928 Size: 971 725 1456 429 393 295 1625 5483 Fragments arranged by size: 5483 1625 1456 971 725 429 393 295 BbvCI CC'TCA_GC Cuts at: 4028 5573 5627 5708 5735 5762 5843 5978 6113 Size: 1545 54 81 27 27 81 135 135 Cuts at: 6113 6302 4028 Size: 189 9103 Fragments arranged by size: 9103 1545 189 135 135 81 81 54 27 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 9568 9602 1903 Size: 34 2339 34 5258 34 3678 Fragments arranged by size: 5258 3678 2339 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 4536 9269 10479 4067 Size: 469 4733 1210 4965 Fragments arranged by size: 4965 4733 1210 469 BclI T'GATC_A Cuts at: 1548 3417 4920 5410 6385 1548 Size: 1869 1503 490 975 6540 Fragments arranged by size: 6540 1869 1503 975 490 BfrBI ATG'CAT Cuts at: 4132 4132 Size: 11377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7181 9988 136 Size: 107 122 71 631 605 5509 2807 1525 Fragments arranged by size: 5509 2807 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6190 6259 8019 11372 6190 Size: 69 1760 3353 6195 Fragments arranged by size: 6195 3353 1760 69 BlpI GC'TnA_GC Cuts at: 4593 8794 4593 Size: 4201 7176 Bme1580I G_kGCm'C Cuts at: 1790 2051 2571 2752 2909 3891 5370 6639 8308 Size: 261 520 181 157 982 1479 1269 1669 Cuts at: 8308 9438 10594 1790 Size: 1130 1156 2573 Fragments arranged by size: 2573 1669 1479 1269 1156 1130 982 520 261 181 157 BmgBI CAC'GTC Cuts at: 1318 3529 8327 1318 Size: 2211 4798 4368 Fragments arranged by size: 4798 4368 2211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3493 3894 5430 Size: 416 53 83 399 24 2076 401 1536 Cuts at: 5430 7807 8299 9108 10066 442 Size: 2377 492 809 958 1753 Fragments arranged by size: 2377 2076 1753 1536 958 809 492 416 401 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3311 3311 Size: 11377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2418 2695 2852 4083 4298 4587 5331 5439 Size: 1179 277 157 1231 215 289 744 108 Cuts at: 5439 5550 5577 5604 5631 5685 5712 5766 5793 Size: 111 27 27 27 54 27 54 27 Cuts at: 5793 5820 5874 5901 5955 6009 6063 6117 6225 Size: 27 54 27 54 54 54 54 108 Cuts at: 6225 6306 6360 6670 10037 1239 Size: 81 54 310 3367 2579 Fragments arranged by size: 3367 2579 1231 1179 744 310 289 277 215 157 111 108 108 81 54 54 54 54 54 54 54 54 27 27 27 27 27 27 27 Bpu10I CC'TnA_GC Cuts at: 2096 2118 2145 4028 4685 5573 5627 5708 5735 Size: 22 27 1883 657 888 54 81 27 Cuts at: 5735 5762 5843 5978 6113 6302 2096 Size: 27 81 135 135 189 7171 Fragments arranged by size: 7171 1883 888 657 189 135 135 81 81 54 27 27 27 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2143 2331 2353 3701 5495 8372 9502 10367 10665 Size: 188 22 1348 1794 2877 1130 865 298 Cuts at: 10665 2143 Size: 2855 Fragments arranged by size: 2877 2855 1794 1348 1130 865 298 188 22 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2857 3138 10040 882 Size: 617 1358 281 6902 2219 Fragments arranged by size: 6902 2219 1358 617 281 BsaAI yAC'GTr Cuts at: 493 1204 2110 4943 7129 7525 9168 493 Size: 711 906 2833 2186 396 1643 2702 Fragments arranged by size: 2833 2702 2186 1643 906 711 396 BsaBI GATnn'nnATC Cuts at: 11371 11371 Size: 11377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 9184 9566 10201 275 Size: 53 83 186 936 7651 382 635 1451 Fragments arranged by size: 7651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4709 9810 10208 10482 10629 11040 4709 Size: 5101 398 274 147 411 5046 Fragments arranged by size: 5101 5046 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2452 2482 3939 3969 4628 Size: 30 165 30 1049 30 1457 30 659 Cuts at: 4628 4658 4687 4717 4738 4768 5575 5605 5629 Size: 30 29 30 21 30 807 30 24 Cuts at: 5629 5659 5710 5740 5899 5929 6223 6253 6304 Size: 30 51 30 159 30 294 30 51 Cuts at: 6304 6334 6665 6695 1178 Size: 30 331 30 5860 Fragments arranged by size: 5860 1457 1049 807 659 331 294 165 159 51 51 30 30 30 30 30 30 30 30 30 30 30 30 30 30 29 24 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 6363 6673 1186 Size: 5177 310 5890 Fragments arranged by size: 5890 5177 310 BseYI C'CCAG_C Cuts at: 2055 2064 2776 2957 5674 5944 6052 6086 10580 Size: 9 712 181 2717 270 108 34 4494 Cuts at: 10580 11076 2055 Size: 496 2356 Fragments arranged by size: 4494 2717 2356 712 496 270 181 108 34 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 10804 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8827 9588 10616 1540 Size: 275 186 6826 761 1028 2301 Fragments arranged by size: 6826 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2789 3767 5463 9438 9523 10594 2789 Size: 978 1696 3975 85 1071 3572 Fragments arranged by size: 3975 3572 1696 1071 978 85 BsmI GAATG_Cn' Cuts at: 4483 4939 5200 8320 8338 8888 8981 4483 Size: 456 261 3120 18 550 93 6879 Fragments arranged by size: 6879 3120 550 456 261 93 18 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2151 2571 2752 2789 2909 3280 Size: 399 261 100 420 181 37 120 371 Cuts at: 3280 3767 3891 5370 5463 6639 7806 8308 8696 Size: 487 124 1479 93 1176 1167 502 388 Cuts at: 8696 9438 9523 10594 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1479 1176 1167 1071 742 502 487 420 399 388 371 261 181 124 120 100 93 85 37 BspEI T'CCGG_A Cuts at: 4709 11040 4709 Size: 6331 5046 BspHI T'CATG_A Cuts at: 3537 3759 9266 10996 3537 Size: 222 5507 1730 3918 Fragments arranged by size: 5507 3918 1730 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 7500 8732 844 Size: 6656 1232 3489 Fragments arranged by size: 6656 3489 1232 BsrBI CCG'CTC Cuts at: 1394 1823 9264 1394 Size: 429 7441 3507 Fragments arranged by size: 7441 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3063 5258 6419 6702 6753 9872 10046 Size: 1183 1815 2195 1161 283 51 3119 174 Cuts at: 10046 65 Size: 1396 Fragments arranged by size: 3119 2195 1815 1396 1183 1161 283 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 10021 10208 1700 Size: 110 8211 187 2869 Fragments arranged by size: 8211 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2697 3189 6793 96 Size: 2601 492 3604 4680 Fragments arranged by size: 4680 3604 2601 492 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11377 BssSI C'ACGA_G Cuts at: 3024 9437 10449 3024 Size: 6413 1012 3952 Fragments arranged by size: 6413 3952 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2638 7874 794 Size: 1844 5236 4297 Fragments arranged by size: 5236 4297 1844 BstBI TT'CG_AA Cuts at: 6865 10181 6865 Size: 3316 8061 BstEII G'GTnAC_C Cuts at: 1184 5581 1184 Size: 4397 6980 BstXI CCAn_nnnn'nTGG Cuts at: 2028 3884 4697 4748 8212 2028 Size: 1856 813 51 3464 5193 Fragments arranged by size: 5193 3464 1856 813 51 BstYI r'GATC_y Cuts at: 1972 3590 4516 5444 6190 6259 6352 6691 7832 Size: 1618 926 928 746 69 93 339 1141 Cuts at: 7832 7890 7929 8019 8130 8707 9074 9459 9476 Size: 58 39 90 111 577 367 385 17 Cuts at: 9476 10928 11014 11026 11372 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1618 1452 1141 928 926 746 577 385 367 346 339 111 93 90 86 69 58 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3397 3397 Size: 11377 BtgI C'CryG_G Cuts at: 513 1065 7021 8520 513 Size: 552 5956 1499 3370 Fragments arranged by size: 5956 3370 1499 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3532 487 Size: 683 56 2306 8332 Fragments arranged by size: 8332 2306 683 56 BtsI GCAGTG_nn' Cuts at: 6610 8372 8893 9687 9707 6610 Size: 1762 521 794 20 8280 Fragments arranged by size: 8280 1762 794 521 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11342 DraI TTT'AAA Cuts at: 2311 6985 7612 8681 2311 Size: 4674 627 1069 5007 Fragments arranged by size: 5007 4674 1069 627 DraIII CAC_nnn'GTG Cuts at: 4562 4562 Size: 11377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10384 809 Size: 507 9068 1802 Fragments arranged by size: 9068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3318 4993 Size: 54 1425 50 275 18 991 497 1675 Cuts at: 4993 7540 8824 9713 11342 8 Size: 2547 1284 889 1629 43 Fragments arranged by size: 2547 1675 1629 1425 1284 991 889 497 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8824 1537 Size: 275 7012 4090 Fragments arranged by size: 7012 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3041 3391 3938 4152 4245 5241 5455 6585 Size: 1688 350 547 214 93 996 214 1130 Cuts at: 6585 6912 7337 9307 1353 Size: 327 425 1970 3423 Fragments arranged by size: 3423 1970 1688 1130 996 547 425 350 327 214 214 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9964 10338 10484 387 Size: 1064 138 8375 374 146 1280 Fragments arranged by size: 8375 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2787 5461 2787 Size: 2674 8703 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2418 2695 2852 4083 4170 4298 Size: 393 669 117 277 157 1231 87 128 Cuts at: 4298 4521 4587 5084 5331 5412 5439 5550 5577 Size: 223 66 497 247 81 27 111 27 Cuts at: 5577 5604 5631 5685 5712 5766 5793 5820 5854 Size: 27 27 54 27 54 27 27 34 Cuts at: 5854 5874 5901 5955 5989 6009 6063 6117 6225 Size: 20 27 54 34 20 54 54 108 Cuts at: 6225 6306 6360 6670 6783 6975 7764 7979 9440 Size: 81 54 310 113 192 789 215 1461 Cuts at: 9440 10037 10824 11092 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1461 1231 789 787 669 597 497 393 310 277 268 247 223 215 192 157 128 117 113 111 108 87 81 81 66 54 54 54 54 54 54 34 34 27 27 27 27 27 27 27 27 20 20 EcoNI CCTnn'n_nnAGG Cuts at: 2600 3400 5576 6305 2600 Size: 800 2176 729 7672 Fragments arranged by size: 7672 2176 800 729 EcoO109I rG'GnC_Cy Cuts at: 2032 2568 2592 2827 3263 3852 4604 4719 4729 Size: 536 24 235 436 589 752 115 10 Cuts at: 4729 4770 4780 5142 7241 2032 Size: 41 10 362 2099 6168 Fragments arranged by size: 6168 2099 752 589 536 436 362 235 115 41 24 10 10 EcoRI G'AATT_C Cuts at: 4003 4292 4571 5311 6930 8686 9064 4003 Size: 289 279 740 1619 1756 378 6316 Fragments arranged by size: 6316 1756 1619 740 378 289 279 EcoRV GAT'ATC Cuts at: 6366 6366 Size: 11377 FspI TGC'GCA Cuts at: 9883 9883 Size: 11377 HaeII r_GCGC'y Cuts at: 1678 10524 1678 Size: 8846 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2326 2358 2537 2569 3137 Size: 32 135 32 754 32 179 32 568 Cuts at: 3137 3169 3247 3267 3279 3299 3536 3568 4061 Size: 32 78 20 12 20 237 32 493 Cuts at: 4061 4093 5575 5607 5629 5661 5899 5931 6223 Size: 32 1482 32 22 32 238 32 292 Cuts at: 6223 6255 6304 6336 6355 6387 6463 6495 7211 Size: 32 49 32 19 32 76 32 716 Cuts at: 7211 7243 7296 7328 10018 10050 10092 10124 1373 Size: 32 53 32 2690 32 42 32 2626 Fragments arranged by size: 2690 2626 1482 754 716 568 493 292 238 237 179 135 78 76 53 49 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 22 20 20 19 12 HincII GTy'rAC Cuts at: 669 1657 1938 3382 3993 4909 8721 8967 669 Size: 988 281 1444 611 916 3812 246 3079 Fragments arranged by size: 3812 3079 1444 988 916 611 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 11377 HpaI GTT'AAC Cuts at: 3993 4909 8967 3993 Size: 916 4058 6403 Fragments arranged by size: 6403 4058 916 KpnI G_GTAC'C Cuts at: 3679 6718 8702 3679 Size: 3039 1984 6354 Fragments arranged by size: 6354 3039 1984 MfeI C'AATT_G Cuts at: 4912 8976 4912 Size: 4064 7313 MluI A'CGCG_T Cuts at: 11253 11253 Size: 11377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2111 2266 3958 7648 8025 8162 10491 10675 Size: 806 155 1692 3690 377 137 2329 184 Cuts at: 10675 1305 Size: 2007 Fragments arranged by size: 3690 2329 2007 1692 806 377 184 155 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 3320 4995 10 Size: 54 1425 343 1488 1675 6392 Fragments arranged by size: 6392 1675 1488 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 7020 7292 7523 8475 9335 9694 9853 Size: 648 5854 272 231 952 860 359 159 Cuts at: 9853 518 Size: 2042 Fragments arranged by size: 5854 2042 952 860 648 359 272 231 159 MspA1I CmG'CkG Cuts at: 1606 2055 2766 4421 4904 7202 8793 9470 10618 Size: 449 711 1655 483 2298 1591 677 1148 Cuts at: 10618 10863 1606 Size: 245 2120 Fragments arranged by size: 2298 2120 1655 1591 1148 711 677 483 449 245 NcoI C'CATG_G Cuts at: 513 1065 7021 8520 513 Size: 552 5956 1499 3370 Fragments arranged by size: 5956 3370 1499 552 NdeI CA'TA_TG Cuts at: 387 4081 5904 387 Size: 3694 1823 5860 Fragments arranged by size: 5860 3694 1823 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11377 NotI GC'GGCC_GC Cuts at: 8824 8824 Size: 11377 NsiI A_TGCA'T Cuts at: 4134 4134 Size: 11377 NspI r_CATG'y Cuts at: 1132 1229 4592 7019 7139 7291 7906 8567 8735 Size: 97 3363 2427 120 152 615 661 168 Cuts at: 8735 10280 1132 Size: 1545 2229 Fragments arranged by size: 3363 2427 2229 1545 661 615 168 152 120 97 PasI CC'CwG_GG Cuts at: 1348 4722 8212 8320 1348 Size: 3374 3490 108 4405 Fragments arranged by size: 4405 3490 3374 108 PciI A'CATG_T Cuts at: 8563 10276 8563 Size: 1713 9664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 6248 6275 6464 1108 Size: 1722 3418 27 189 6021 Fragments arranged by size: 6021 3418 1722 189 27 PfoI T'CCnGG_A Cuts at: 2829 4646 6348 2829 Size: 1817 1702 7858 Fragments arranged by size: 7858 1817 1702 PmeI GTTT'AAAC Cuts at: 8681 8681 Size: 11377 PmlI CAC'GTG Cuts at: 4943 7525 4943 Size: 2582 8795 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9446 9478 10959 10991 9446 Size: 32 1481 32 9832 Fragments arranged by size: 9832 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2592 2827 3263 3852 4604 4729 4780 5142 Size: 560 235 436 589 752 125 51 362 Cuts at: 5142 7241 2032 Size: 2099 6168 Fragments arranged by size: 6168 2099 752 589 560 436 362 235 125 51 PshAI GACnn'nnGTC Cuts at: 1613 5328 5436 5487 6465 1613 Size: 3715 108 51 978 6525 Fragments arranged by size: 6525 3715 978 108 51 PsiI TTA'TAA Cuts at: 3177 3453 7839 8414 8947 3177 Size: 276 4386 575 533 5607 Fragments arranged by size: 5607 4386 575 533 276 PspOMI G'GGCC_C Cuts at: 2567 2905 2567 Size: 338 11039 PspXI vC'TCGA_Gb Cuts at: 6903 8142 6903 Size: 1239 10138 PstI C_TGCA'G Cuts at: 830 3073 4201 8729 830 Size: 2243 1128 4528 3478 Fragments arranged by size: 4528 3478 2243 1128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11377 PvuII CAG'CTG Cuts at: 2055 2766 4421 4904 7202 2055 Size: 711 1655 483 2298 6230 Fragments arranged by size: 6230 2298 1655 711 483 SacI G_AGCT'C Cuts at: 2789 5463 2789 Size: 2674 8703 SalI G'TCGA_C Cuts at: 1936 8719 1936 Size: 6783 4594 SanDI GG'GwC_CC Cuts at: 2592 3852 4604 2592 Size: 1260 752 9365 Fragments arranged by size: 9365 1260 752 SapI GCTCTTCn'nnn_ Cuts at: 6912 7337 6912 Size: 425 10952 SbfI CC_TGCA'GG Cuts at: 8729 8729 Size: 11377 ScaI AGT'ACT Cuts at: 9625 11337 9625 Size: 1712 9665 SfcI C'TryA_G Cuts at: 826 1045 2200 3069 3331 3520 3841 4107 4197 Size: 219 1155 869 262 189 321 266 90 Cuts at: 4197 4509 4523 7441 7723 8473 8725 9860 10541 Size: 312 14 2918 282 750 252 1135 681 Cuts at: 10541 10732 826 Size: 191 1471 Fragments arranged by size: 2918 1471 1155 1135 869 750 681 321 312 282 266 262 252 219 191 189 90 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11377 SmaI CCC'GGG Cuts at: 1872 8704 1872 Size: 6832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2158 2332 2346 3011 3680 5195 Size: 197 926 215 174 14 665 669 1515 Cuts at: 5195 5474 6903 8142 8387 9481 10382 10644 820 Size: 279 1429 1239 245 1094 901 262 1553 Fragments arranged by size: 1553 1515 1429 1239 1094 926 901 669 665 279 262 245 215 197 174 14 SnaBI TAC'GTA Cuts at: 493 7129 9168 493 Size: 6636 2039 2702 Fragments arranged by size: 6636 2702 2039 SpeI A'CTAG_T Cuts at: 152 152 Size: 11377 SphI G_CATG'C Cuts at: 1132 7139 8735 1132 Size: 6007 1596 3774 Fragments arranged by size: 6007 3774 1596 SspI AAT'ATT Cuts at: 5 52 4351 9301 5 Size: 47 4299 4950 2081 Fragments arranged by size: 4950 4299 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 11377 StyI C'CwwG_G Cuts at: 513 1065 2074 4608 6247 6463 7021 7886 7939 Size: 552 1009 2534 1639 216 558 865 53 Cuts at: 7939 8346 8520 8816 513 Size: 407 174 296 3074 Fragments arranged by size: 3074 2534 1639 1009 865 558 552 407 296 216 174 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 5013 5499 5672 5807 5942 6050 Size: 1255 921 2512 486 173 135 135 108 Cuts at: 6050 7468 9404 9421 9574 9759 10204 325 Size: 1418 1936 17 153 185 445 1498 Fragments arranged by size: 2512 1936 1498 1418 1255 921 486 445 185 173 153 135 135 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 3189 6793 Size: 274 80 33 51 1443 720 492 3604 Cuts at: 6793 9623 11335 96 Size: 2830 1712 138 Fragments arranged by size: 3604 2830 1712 1443 720 492 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2452 2484 3939 3971 4628 4660 4687 4719 4738 Size: 32 1455 32 657 32 27 32 19 Cuts at: 4738 4770 11337 11369 2452 Size: 32 6567 32 2460 Fragments arranged by size: 6567 2460 1455 657 32 32 32 32 32 32 27 19 Tth111I GACn'n_nGTC Cuts at: 1316 3824 1316 Size: 2508 8869 XbaI T'CTAG_A Cuts at: 5863 5998 6106 8713 5863 Size: 135 108 2607 8527 Fragments arranged by size: 8527 2607 135 108 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2916 2916 Size: 11377 XhoI C'TCGA_G Cuts at: 1943 6903 8142 1943 Size: 4960 1239 5178 Fragments arranged by size: 5178 4960 1239 XmaI C'CCGG_G Cuts at: 1870 8702 1870 Size: 6832 4545 XmnI GAAnn'nnTTC Cuts at: 5655 5668 8576 9506 5655 Size: 13 2908 930 7526 Fragments arranged by size: 7526 2908 930 13 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9185 276 Size: 53 83 186 936 7651 2468 Fragments arranged by size: 7651 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI