(Circular) (Six-base) MAPSORT of: pFN21AB4815.seq Check: 9513 from: 1 to: 9127 pFN21AB4815 9127 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3097 3765 4241 3097 Size: 668 476 7983 Fragments arranged by size: 7983 668 476 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6937 278 Size: 53 83 186 936 5401 2468 Fragments arranged by size: 5401 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 4111 5970 6470 1937 Size: 562 1612 1859 500 4594 Fragments arranged by size: 4594 1859 1612 562 500 Acc65I G'GTAC_C Cuts at: 3251 6448 3251 Size: 3197 5930 AclI AA'CG_TT Cuts at: 1563 7254 7627 1563 Size: 5691 373 3063 Fragments arranged by size: 5691 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2957 3494 3703 4125 4344 4653 4746 4809 Size: 1325 537 209 422 219 309 93 63 Cuts at: 4809 5162 5835 6200 7190 8574 8842 1632 Size: 353 673 365 990 1384 268 1917 Fragments arranged by size: 1917 1384 1325 990 673 537 422 365 353 309 268 219 209 93 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8930 AflIII A'CryG_T Cuts at: 1203 2791 4114 8026 9003 1203 Size: 1588 1323 3912 977 1327 Fragments arranged by size: 3912 1588 1327 1323 977 AgeI A'CCGG_T Cuts at: 7958 7958 Size: 9127 AhdI GACnn_n'nnGTC Cuts at: 7856 7856 Size: 9127 AleI CACnn'nnGTG Cuts at: 1166 5768 1166 Size: 4602 4525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5748 1184 Size: 4564 4563 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2291 2323 2291 Size: 32 9095 AlwNI CAG_nnn'CTG Cuts at: 2081 2180 4280 4676 6046 6283 6870 8442 2081 Size: 99 2100 396 1370 237 587 1572 2766 Fragments arranged by size: 2766 2100 1572 1370 587 396 237 99 ApaLI G'TGCA_C Cuts at: 7184 8340 7184 Size: 1156 7971 ApoI r'AATT_y Cuts at: 1493 2342 2438 2672 2870 3090 4609 5870 6436 Size: 849 96 234 198 220 1519 1261 566 Cuts at: 6436 6662 6814 8923 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1519 1261 849 566 234 226 220 198 152 96 AseI AT'TA_AT Cuts at: 160 1032 3954 7681 160 Size: 872 2922 3727 1606 Fragments arranged by size: 3727 2922 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9127 AvaI C'yCGr_G Cuts at: 1870 1943 4836 5160 6452 1870 Size: 73 2893 324 1292 4545 Fragments arranged by size: 4545 2893 1292 324 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3960 3993 3960 Size: 33 9094 BamHI G'GATC_C Cuts at: 2509 6457 6824 2509 Size: 3948 367 4812 Fragments arranged by size: 4812 3948 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3251 3458 4663 5575 6448 Size: 325 217 627 1464 207 1205 912 873 Cuts at: 6448 7903 618 Size: 1455 1842 Fragments arranged by size: 1842 1464 1455 1205 912 873 627 325 217 207 BanII G_rGCy'C Cuts at: 1391 2598 2609 3393 3843 4392 5252 6446 1391 Size: 1207 11 784 450 549 860 1194 4072 Fragments arranged by size: 4072 1207 1194 860 784 549 450 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2277 2920 3564 3930 5150 5408 5841 Size: 971 378 643 644 366 1220 258 433 Cuts at: 5841 928 Size: 4214 Fragments arranged by size: 4214 1220 971 644 643 433 378 366 258 BbvCI CC'TCA_GC Cuts at: 4260 5616 4260 Size: 1356 7771 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7318 7352 1903 Size: 34 5381 34 3678 Fragments arranged by size: 5381 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7019 8229 7019 Size: 1210 7917 BclI T'GATC_A Cuts at: 1548 1548 Size: 9127 BfrBI ATG'CAT Cuts at: 4096 4467 4096 Size: 371 8756 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7738 136 Size: 107 122 71 631 605 6066 1525 Fragments arranged by size: 6066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6206 6325 6412 9122 6206 Size: 119 87 2710 6211 Fragments arranged by size: 6211 2710 119 87 BlpI GC'TnA_GC Cuts at: 3837 6544 3837 Size: 2707 6420 Bme1580I G_kGCm'C Cuts at: 1790 7188 8344 1790 Size: 5398 1156 2573 Fragments arranged by size: 5398 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3655 1318 Size: 2337 6790 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4511 5829 Size: 416 53 83 399 24 2120 974 1318 Cuts at: 5829 6311 6858 7816 442 Size: 482 547 958 1753 Fragments arranged by size: 2120 1753 1318 974 958 547 482 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 3957 4886 4980 5354 5408 5960 7787 Size: 1016 1702 929 94 374 54 552 1827 Cuts at: 7787 1239 Size: 2579 Fragments arranged by size: 2579 1827 1702 1016 929 552 374 94 54 Bpu10I CC'TnA_GC Cuts at: 4260 5616 4260 Size: 1356 7771 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2549 3407 4418 4626 5489 7252 8117 8415 2549 Size: 858 1011 208 863 1763 865 298 3261 Fragments arranged by size: 3261 1763 1011 865 863 858 298 208 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 4184 5917 6166 7790 882 Size: 617 624 2061 1733 249 1624 2219 Fragments arranged by size: 2219 2061 1733 1624 624 617 249 BsaAI yAC'GTr Cuts at: 493 1204 4115 6918 493 Size: 711 2911 2803 2702 Fragments arranged by size: 2911 2803 2702 711 BsaBI GATnn'nnATC Cuts at: 9121 9121 Size: 9127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 3132 3848 6934 Size: 53 83 186 936 537 1062 716 3086 Cuts at: 6934 7316 7951 275 Size: 382 635 1451 Fragments arranged by size: 3086 1451 1062 936 716 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7560 7958 8232 8379 8790 7560 Size: 398 274 147 411 7897 Fragments arranged by size: 7897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2291 2321 3170 3200 3504 Size: 30 165 30 888 30 849 30 304 Cuts at: 3504 3534 3720 3750 3757 3787 3955 3985 4260 Size: 30 186 30 7 30 168 30 275 Cuts at: 4260 4290 1178 Size: 30 6015 Fragments arranged by size: 6015 888 849 304 275 186 168 165 30 30 30 30 30 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2312 3765 5298 5633 5740 1186 Size: 850 276 1453 1533 335 107 4573 Fragments arranged by size: 4573 1533 1453 850 335 276 107 BseYI C'CCAG_C Cuts at: 3466 3739 5661 8330 8826 3466 Size: 273 1922 2669 496 3767 Fragments arranged by size: 3767 2669 1922 496 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4502 5814 6059 1163 Size: 573 2766 1312 245 4231 Fragments arranged by size: 4231 2766 1312 573 245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6144 6577 7338 8366 1540 Size: 275 186 4143 433 761 1028 2301 Fragments arranged by size: 4143 2301 1028 761 433 275 186 BsiHKAI G_wGCw'C Cuts at: 2655 3843 4392 5252 5643 7188 7273 8344 2655 Size: 1188 549 860 391 1545 85 1071 3438 Fragments arranged by size: 3438 1545 1188 1071 860 549 391 85 BsiWI C'GTAC_G Cuts at: 4195 4195 Size: 9127 BsmI GAATG_Cn' Cuts at: 4034 5737 6638 6731 4034 Size: 1703 901 93 6430 Fragments arranged by size: 6430 1703 901 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6359 6359 Size: 9127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2598 2609 2655 3393 3843 4392 5252 Size: 399 808 11 46 738 450 549 860 Cuts at: 5252 5643 6446 7188 7273 8344 1391 Size: 391 803 742 85 1071 2174 Fragments arranged by size: 2174 1071 860 808 803 742 738 549 450 399 391 85 46 11 BspEI T'CCGG_A Cuts at: 8790 8790 Size: 9127 BspHI T'CATG_A Cuts at: 3400 3822 7016 8746 3400 Size: 422 3194 1730 3781 Fragments arranged by size: 3781 3194 1730 422 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3097 3765 3873 4241 5465 5899 6482 844 Size: 2253 668 108 368 1224 434 583 3489 Fragments arranged by size: 3489 2253 1224 668 583 434 368 108 BsrBI CCG'CTC Cuts at: 1394 1823 6123 7014 1394 Size: 429 4300 891 3507 Fragments arranged by size: 4300 3507 891 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3603 5377 5915 7622 7796 65 Size: 1183 2355 1774 538 1707 174 1396 Fragments arranged by size: 2355 1774 1707 1396 1183 538 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7771 7958 1700 Size: 110 5961 187 2869 Fragments arranged by size: 5961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3217 4002 96 Size: 3121 785 5221 Fragments arranged by size: 5221 3121 785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9127 BssSI C'ACGA_G Cuts at: 7187 8199 7187 Size: 1012 8115 BstAPI GCAn_nnn'nTGC Cuts at: 794 6046 794 Size: 5252 3875 BstBI TT'CG_AA Cuts at: 7931 7931 Size: 9127 BstEII G'GTnAC_C Cuts at: 1184 5036 5477 1184 Size: 3852 441 4834 Fragments arranged by size: 4834 3852 441 BstXI CCAn_nnnn'nTGG Cuts at: 2365 3247 3706 2365 Size: 882 459 7786 Fragments arranged by size: 7786 882 459 BstYI r'GATC_y Cuts at: 1972 2509 2979 4221 6206 6325 6412 6457 6824 Size: 537 470 1242 1985 119 87 45 367 Cuts at: 6824 7209 7226 8678 8764 8776 9122 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1985 1977 1452 1242 537 470 385 367 346 119 87 86 45 17 12 BstZ17I GTA'TAC Cuts at: 2500 2500 Size: 9127 BtgI C'CryG_G Cuts at: 513 1065 2004 2454 2664 2839 2893 3198 4440 Size: 552 939 450 210 175 54 305 1242 Cuts at: 4440 5422 513 Size: 982 4218 Fragments arranged by size: 4218 1242 982 939 552 450 305 210 175 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2512 5308 487 Size: 683 56 1286 2796 4306 Fragments arranged by size: 4306 2796 1286 683 56 BtsI GCAGTG_nn' Cuts at: 3342 4350 4540 5258 5447 6643 7437 7457 3342 Size: 1008 190 718 189 1196 794 20 5012 Fragments arranged by size: 5012 1196 1008 794 718 190 189 20 ClaI AT'CG_AT Cuts at: 1554 5178 1554 Size: 3624 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9092 DraI TTT'AAA Cuts at: 6431 6431 Size: 9127 DraIII CAC_nnn'GTG Cuts at: 4296 4296 Size: 9127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2075 2387 4107 5543 5845 8134 809 Size: 507 759 312 1720 1436 302 2289 1802 Fragments arranged by size: 2289 1802 1720 1436 759 507 312 302 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3415 3696 6574 Size: 54 1425 50 275 18 1585 281 2878 Cuts at: 6574 7463 9092 8 Size: 889 1629 43 Fragments arranged by size: 2878 1629 1585 1425 889 281 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6574 1537 Size: 275 4762 4090 Fragments arranged by size: 4762 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 2730 2933 3324 4141 4170 4351 6009 Size: 875 502 203 391 817 29 181 1658 Cuts at: 6009 7057 1353 Size: 1048 3423 Fragments arranged by size: 3423 1658 1048 875 817 502 391 203 181 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 5299 7714 8088 8234 387 Size: 1064 138 666 3044 2415 374 146 1280 Fragments arranged by size: 3044 2415 1280 1064 666 374 146 138 EcoICRI GAG'CTC Cuts at: 3841 4390 5250 3841 Size: 549 860 7718 Fragments arranged by size: 7718 860 549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2957 3494 3703 3957 4125 4344 Size: 393 623 702 537 209 254 168 219 Cuts at: 4344 4653 4746 4809 4886 4980 5162 5354 5408 Size: 309 93 63 77 94 182 192 54 Cuts at: 5408 5835 5960 6200 7190 7787 8574 8842 1239 Size: 427 125 240 990 597 787 268 1524 Fragments arranged by size: 1524 990 787 702 623 597 537 427 393 309 268 254 240 219 209 192 182 168 125 94 93 77 63 54 EcoNI CCTnn'n_nnAGG Cuts at: 6263 6263 Size: 9127 EcoO109I rG'GnC_Cy Cuts at: 3880 4308 5683 3880 Size: 428 1375 7324 Fragments arranged by size: 7324 1375 428 EcoRI G'AATT_C Cuts at: 3090 6436 6814 3090 Size: 3346 378 5403 Fragments arranged by size: 5403 3346 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3502 3502 Size: 9127 FspI TGC'GCA Cuts at: 7633 7633 Size: 9127 HaeII r_GCGC'y Cuts at: 1678 5560 8274 1678 Size: 3882 2714 2531 Fragments arranged by size: 3882 2714 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3380 3412 3504 3536 4260 Size: 32 135 32 1808 32 92 32 724 Cuts at: 4260 4292 4326 4358 4830 4862 4991 5023 5154 Size: 32 34 32 472 32 129 32 131 Cuts at: 5154 5186 5615 5647 5675 5707 6034 6066 6254 Size: 32 429 32 28 32 327 32 188 Cuts at: 6254 6286 7768 7800 7842 7874 1373 Size: 32 1482 32 42 32 2626 Fragments arranged by size: 2626 1808 1482 724 472 429 327 188 135 131 129 92 42 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 6471 6717 669 Size: 988 281 4533 246 3079 Fragments arranged by size: 4533 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9127 HpaI GTT'AAC Cuts at: 6717 6717 Size: 9127 KpnI G_GTAC'C Cuts at: 3255 6452 3255 Size: 3197 5930 MfeI C'AATT_G Cuts at: 6381 6726 6381 Size: 345 8782 MluI A'CGCG_T Cuts at: 9003 9003 Size: 9127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2856 6156 8241 8425 1305 Size: 1551 3300 2085 184 2007 Fragments arranged by size: 3300 2085 2007 1551 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3417 3698 10 Size: 54 1425 343 1585 281 5439 Fragments arranged by size: 5439 1585 1425 343 281 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 5067 5768 5948 7085 7444 7603 Size: 648 1197 2704 701 180 1137 359 159 Cuts at: 7603 518 Size: 2042 Fragments arranged by size: 2704 2042 1197 1137 701 648 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2935 3770 4486 6543 7220 8368 8613 1606 Size: 1329 835 716 2057 677 1148 245 2120 Fragments arranged by size: 2120 2057 1329 1148 835 716 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2454 2893 5422 513 Size: 552 939 450 439 2529 4218 Fragments arranged by size: 4218 2529 939 552 450 439 NdeI CA'TA_TG Cuts at: 387 2770 5207 387 Size: 2383 2437 4307 Fragments arranged by size: 4307 2437 2383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9127 NotI GC'GGCC_GC Cuts at: 6574 6574 Size: 9127 NsiI A_TGCA'T Cuts at: 4098 4469 4098 Size: 371 8756 NspI r_CATG'y Cuts at: 1132 1229 2795 4172 6485 8030 1132 Size: 97 1566 1377 2313 1545 2229 Fragments arranged by size: 2313 2229 1566 1545 1377 97 PasI CC'CwG_GG Cuts at: 1348 3896 5737 1348 Size: 2548 1841 4738 Fragments arranged by size: 4738 2548 1841 PciI A'CATG_T Cuts at: 2791 8026 2791 Size: 5235 3892 PflMI CCAn_nnn'nTGG Cuts at: 1108 4775 1108 Size: 3667 5460 PmeI GTTT'AAAC Cuts at: 6431 6431 Size: 9127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2323 3517 3549 7196 7228 8709 8741 2291 Size: 32 1194 32 3647 32 1481 32 2677 Fragments arranged by size: 3647 2677 1481 1194 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3880 5683 3880 Size: 1803 7324 PshAI GACnn'nnGTC Cuts at: 1613 6230 1613 Size: 4617 4510 PsiI TTA'TAA Cuts at: 6697 6697 Size: 9127 PspXI vC'TCGA_Gb Cuts at: 5160 5160 Size: 9127 PstI C_TGCA'G Cuts at: 830 5907 6479 830 Size: 5077 572 3478 Fragments arranged by size: 5077 3478 572 PvuI CG_AT'CG Cuts at: 2001 6144 2001 Size: 4143 4984 PvuII CAG'CTG Cuts at: 2935 4486 2935 Size: 1551 7576 SacI G_AGCT'C Cuts at: 3843 4392 5252 3843 Size: 549 860 7718 Fragments arranged by size: 7718 860 549 SalI G'TCGA_C Cuts at: 1936 6469 1936 Size: 4533 4594 SapI GCTCTTCn'nnn_ Cuts at: 4170 4170 Size: 9127 SbfI CC_TGCA'GG Cuts at: 6479 6479 Size: 9127 ScaI AGT'ACT Cuts at: 4205 5502 7375 9087 4205 Size: 1297 1873 1712 4245 Fragments arranged by size: 4245 1873 1712 1297 SfcI C'TryA_G Cuts at: 826 1045 5077 5903 6475 7610 8291 8482 826 Size: 219 4032 826 572 1135 681 191 1471 Fragments arranged by size: 4032 1471 1135 826 681 572 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9127 SmaI CCC'GGG Cuts at: 1872 6454 1872 Size: 4582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2564 3386 4433 4641 5160 5504 Size: 197 926 621 822 1047 208 519 344 Cuts at: 5504 7231 8132 8394 820 Size: 1727 901 262 1553 Fragments arranged by size: 1727 1553 1047 926 901 822 621 519 344 262 208 197 SnaBI TAC'GTA Cuts at: 493 6918 493 Size: 6425 2702 SpeI A'CTAG_T Cuts at: 152 2494 152 Size: 2342 6785 SphI G_CATG'C Cuts at: 1132 6485 1132 Size: 5353 3774 SspI AAT'ATT Cuts at: 5 52 5998 7051 5 Size: 47 5946 1053 2081 Fragments arranged by size: 5946 2081 1053 47 StuI AGG'CCT Cuts at: 1517 4424 1517 Size: 2907 6220 StyI C'CwwG_G Cuts at: 513 1065 2004 2193 2454 2893 2922 5010 5422 Size: 552 939 189 261 439 29 2088 412 Cuts at: 5422 5571 6566 513 Size: 149 995 3074 Fragments arranged by size: 3074 2088 995 939 552 439 412 261 189 149 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3301 5771 7154 7171 7324 7509 7954 Size: 1255 1721 2470 1383 17 153 185 445 Cuts at: 7954 325 Size: 1498 Fragments arranged by size: 2470 1721 1498 1383 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2695 3217 3983 Size: 274 80 33 51 1443 718 522 766 Cuts at: 3983 4002 4203 4960 5500 6390 7373 9085 96 Size: 19 201 757 540 890 983 1712 138 Fragments arranged by size: 1712 1443 983 890 766 757 718 540 522 274 201 138 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2985 3017 3531 3563 3720 3752 3757 3789 3955 Size: 32 514 32 157 32 5 32 166 Cuts at: 3955 3987 9087 9119 2985 Size: 32 5100 32 2993 Fragments arranged by size: 5100 2993 514 166 157 32 32 32 32 32 32 5 Tth111I GACn'n_nGTC Cuts at: 1316 4697 1316 Size: 3381 5746 XbaI T'CTAG_A Cuts at: 6463 6463 Size: 9127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2555 3244 4910 5329 5449 5578 2555 Size: 689 1666 419 120 129 6104 Fragments arranged by size: 6104 1666 689 419 129 120 XhoI C'TCGA_G Cuts at: 1943 5160 1943 Size: 3217 5910 XmaI C'CCGG_G Cuts at: 1870 6452 1870 Size: 4582 4545 XmnI GAAnn'nnTTC Cuts at: 2466 2676 7256 2466 Size: 210 4580 4337 Fragments arranged by size: 4580 4337 210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6935 276 Size: 53 83 186 936 5401 2468 Fragments arranged by size: 5401 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BplI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI