(Circular) (Six-base) MAPSORT of: pFN21AB4786.seq Check: 2890 from: 1 to: 9097 pFN21AB4786 9097 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2129 5786 2129 Size: 3657 5440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4759 6907 278 Size: 53 83 186 936 3223 2148 2468 Fragments arranged by size: 3223 2468 2148 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6440 1937 Size: 4503 4594 Acc65I G'GTAC_C Cuts at: 2142 6418 2142 Size: 4276 4821 AclI AA'CG_TT Cuts at: 1563 7224 7597 1563 Size: 5661 373 3063 Fragments arranged by size: 5661 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 3114 4166 5581 7160 8544 8812 1632 Size: 996 486 1052 1415 1579 1384 268 1917 Fragments arranged by size: 1917 1579 1415 1384 1052 996 486 268 AfeI AGC'GCT Cuts at: 4606 5584 4606 Size: 978 8119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8900 AflIII A'CryG_T Cuts at: 1203 2157 7996 8973 1203 Size: 954 5839 977 1327 Fragments arranged by size: 5839 1327 977 954 AgeI A'CCGG_T Cuts at: 7928 7928 Size: 9097 AhdI GACnn_n'nnGTC Cuts at: 7826 7826 Size: 9097 AleI CACnn'nnGTG Cuts at: 1166 5637 1166 Size: 4471 4626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9097 AlwNI CAG_nnn'CTG Cuts at: 6840 8412 6840 Size: 1572 7525 ApaI G_GGCC'C Cuts at: 2912 3003 3084 3272 5009 5496 2912 Size: 91 81 188 1737 487 6513 Fragments arranged by size: 6513 1737 487 188 91 81 ApaLI G'TGCA_C Cuts at: 2586 7154 8310 2586 Size: 4568 1156 3373 Fragments arranged by size: 4568 3373 1156 ApoI r'AATT_y Cuts at: 1493 2526 6362 6406 6632 6784 8893 1493 Size: 1033 3836 44 226 152 2109 1697 Fragments arranged by size: 3836 2109 1697 1033 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 7651 160 Size: 872 6619 1606 Fragments arranged by size: 6619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9097 AvaI C'yCGr_G Cuts at: 1870 1943 2168 2264 2361 2661 2760 3084 3246 Size: 73 225 96 97 300 99 324 162 Cuts at: 3246 3417 4092 4850 5424 5690 6422 1870 Size: 171 675 758 574 266 732 4545 Fragments arranged by size: 4545 758 732 675 574 324 300 266 225 171 162 99 97 96 73 BamHI G'GATC_C Cuts at: 4808 6427 6794 4808 Size: 1619 367 7111 Fragments arranged by size: 7111 1619 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2252 2828 3197 3242 Size: 325 217 627 355 110 576 369 45 Cuts at: 3242 3611 3719 4007 4142 4169 4331 4394 5060 Size: 369 108 288 135 27 162 63 666 Cuts at: 5060 5096 5159 5249 6347 6418 7873 618 Size: 36 63 90 1098 71 1455 1842 Fragments arranged by size: 1842 1455 1098 666 627 576 369 369 355 325 288 217 162 135 110 108 90 71 63 63 45 36 27 BanII G_rGCy'C Cuts at: 1391 2175 2896 2912 2950 3003 3084 3272 3418 Size: 784 721 16 38 53 81 188 146 Cuts at: 3418 3780 3859 4156 4390 5009 5056 5496 5747 Size: 362 79 297 234 619 47 440 251 Cuts at: 5747 6032 6182 6416 1391 Size: 285 150 234 4072 Fragments arranged by size: 4072 784 721 619 440 362 297 285 251 234 234 188 150 146 81 79 53 47 38 16 BbeI G_GCGC'C Cuts at: 2256 4011 4173 5100 5253 2256 Size: 1755 162 927 153 6100 Fragments arranged by size: 6100 1755 927 162 153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2109 4208 4919 5863 928 Size: 971 210 2099 711 944 4162 Fragments arranged by size: 4162 2099 971 944 711 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7288 7322 1903 Size: 34 5351 34 3678 Fragments arranged by size: 5351 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6989 8199 6989 Size: 1210 7887 BclI T'GATC_A Cuts at: 1548 1548 Size: 9097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 2364 2391 Size: 107 122 71 631 605 623 69 27 Cuts at: 2391 2400 2746 2916 3132 3276 3555 3915 4437 Size: 9 346 170 216 144 279 360 522 Cuts at: 4437 4797 5175 5184 5247 5373 5751 6047 7708 Size: 360 378 9 63 126 378 296 1661 Cuts at: 7708 136 Size: 1525 Fragments arranged by size: 1661 1525 631 623 605 522 378 378 360 360 346 296 279 216 170 144 126 122 107 71 69 63 27 9 9 BglII A'GATC_T Cuts at: 9092 9092 Size: 9097 BlpI GC'TnA_GC Cuts at: 6514 6514 Size: 9097 Bme1580I G_kGCm'C Cuts at: 1790 2590 2912 3003 3084 3247 3272 3724 4147 Size: 800 322 91 81 163 25 452 423 Cuts at: 4147 4336 4399 5009 5164 5496 6352 7158 8314 Size: 189 63 610 155 332 856 806 1156 Cuts at: 8314 1790 Size: 2573 Fragments arranged by size: 2573 1156 856 806 800 610 452 423 332 322 189 163 155 91 81 63 25 BmgBI CAC'GTC Cuts at: 1318 2158 2221 5045 1318 Size: 840 63 2824 5370 Fragments arranged by size: 5370 2824 840 63 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5913 6828 7786 Size: 416 53 83 399 24 4496 915 958 Cuts at: 7786 442 Size: 1753 Fragments arranged by size: 4496 1753 958 915 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2658 2964 3414 3792 3810 3873 3942 4017 Size: 1419 306 450 378 18 63 69 75 Cuts at: 4017 4044 4170 4404 4602 4989 5336 5421 6158 Size: 27 126 234 198 387 347 85 737 Cuts at: 6158 7757 1239 Size: 1599 2579 Fragments arranged by size: 2579 1599 1419 737 450 387 378 347 306 234 198 126 85 75 69 63 27 18 Bpu10I CC'TnA_GC Cuts at: 3057 3345 3858 4128 5717 3057 Size: 288 513 270 1589 6437 Fragments arranged by size: 6437 1589 513 288 270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4842 5604 5693 5774 6140 7222 8087 8385 4842 Size: 762 89 81 366 1082 865 298 5554 Fragments arranged by size: 5554 1082 865 762 366 298 89 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2228 2573 3941 3993 4398 5111 5480 Size: 617 729 345 1368 52 405 713 369 Cuts at: 5480 5888 7760 882 Size: 408 1872 2219 Fragments arranged by size: 2219 1872 1368 729 713 617 408 405 369 345 52 BsaAI yAC'GTr Cuts at: 493 1204 6888 493 Size: 711 5684 2702 Fragments arranged by size: 5684 2702 711 BsaBI GATnn'nnATC Cuts at: 6194 9091 6194 Size: 2897 6200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2253 2331 4008 4170 Size: 53 83 186 936 720 78 1677 162 Cuts at: 4170 4287 4756 5097 5250 6904 7286 7921 275 Size: 117 469 341 153 1654 382 635 1451 Fragments arranged by size: 1677 1654 1451 936 720 635 469 382 341 186 162 153 117 83 78 53 BsaWI w'CCGG_w Cuts at: 2519 5739 6260 7530 7928 8202 8349 8760 2519 Size: 3220 521 1270 398 274 147 411 2856 Fragments arranged by size: 3220 2856 1270 521 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8872 Fragments arranged by size: 8872 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 3426 4920 6161 1186 Size: 841 1399 1494 1241 4122 Fragments arranged by size: 4122 1494 1399 1241 841 BseYI C'CCAG_C Cuts at: 2487 4361 5579 5610 8300 8796 2487 Size: 1874 1218 31 2690 496 2788 Fragments arranged by size: 2788 2690 1874 1218 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4227 6232 1163 Size: 573 2491 2005 4028 Fragments arranged by size: 4028 2491 2005 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5512 6547 7308 8336 1540 Size: 275 186 3511 1035 761 1028 2301 Fragments arranged by size: 3511 2301 1035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2590 2761 2896 2968 3211 3229 3319 3418 3859 Size: 171 135 72 243 18 90 99 441 Cuts at: 3859 4111 4156 4390 4579 4597 4840 5146 5155 Size: 252 45 234 189 18 243 306 9 Cuts at: 5155 5452 7158 7243 8314 2590 Size: 297 1706 85 1071 3373 Fragments arranged by size: 3373 1706 1071 441 306 297 252 243 243 234 189 171 135 99 90 85 72 45 18 18 9 BsmI GAATG_Cn' Cuts at: 4194 6608 6701 4194 Size: 2414 93 6590 Fragments arranged by size: 6590 2414 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2148 5076 2148 Size: 2928 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2590 2761 2896 2912 2950 2968 Size: 399 385 415 171 135 16 38 18 Cuts at: 2968 3003 3084 3211 3229 3247 3272 3319 3418 Size: 35 81 127 18 18 25 47 99 Cuts at: 3418 3724 3780 3859 4111 4147 4156 4336 4390 Size: 306 56 79 252 36 9 180 54 Cuts at: 4390 4399 4579 4597 4840 5009 5056 5146 5155 Size: 9 180 18 243 169 47 90 9 Cuts at: 5155 5164 5452 5496 5747 6032 6182 6352 6416 Size: 9 288 44 251 285 150 170 64 Cuts at: 6416 7158 7243 8314 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 385 306 288 285 252 251 243 180 180 171 170 169 150 135 127 99 90 85 81 79 64 56 54 47 47 44 38 36 35 25 18 18 18 18 16 9 9 9 9 BspEI T'CCGG_A Cuts at: 5739 8760 5739 Size: 3021 6076 BspHI T'CATG_A Cuts at: 6986 8716 6986 Size: 1730 7367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2129 5786 5906 6452 844 Size: 1285 3657 120 546 3489 Fragments arranged by size: 3657 3489 1285 546 120 BsrBI CCG'CTC Cuts at: 1394 1823 6984 1394 Size: 429 5161 3507 Fragments arranged by size: 5161 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7592 7766 65 Size: 1183 6344 174 1396 Fragments arranged by size: 6344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2327 3014 3680 4301 5120 5228 7741 Size: 110 517 687 666 621 819 108 2513 Cuts at: 7741 7928 1700 Size: 187 2869 Fragments arranged by size: 2869 2513 819 687 666 621 517 187 110 108 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9097 BssSI C'ACGA_G Cuts at: 2562 2967 4389 6250 7157 8169 2562 Size: 405 1422 1861 907 1012 3490 Fragments arranged by size: 3490 1861 1422 1012 907 405 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9097 BstBI TT'CG_AA Cuts at: 7901 7901 Size: 9097 BstEII G'GTnAC_C Cuts at: 1184 5105 5330 1184 Size: 3921 225 4951 Fragments arranged by size: 4951 3921 225 BstXI CCAn_nnnn'nTGG Cuts at: 6146 6146 Size: 9097 BstYI r'GATC_y Cuts at: 1972 2181 3566 4808 6195 6427 6794 7179 7196 Size: 209 1385 1242 1387 232 367 385 17 Cuts at: 7196 8648 8734 8746 9092 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1387 1385 1242 385 367 346 232 209 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2138 2751 2859 4227 2138 Size: 613 108 1368 7008 Fragments arranged by size: 7008 1368 613 108 BtgI C'CryG_G Cuts at: 513 1065 2544 2652 2914 3481 3724 4291 4408 Size: 552 1479 108 262 567 243 567 117 Cuts at: 4408 4732 5001 5110 5254 5281 5335 513 Size: 324 269 109 144 27 54 4275 Fragments arranged by size: 4275 1479 567 567 552 324 269 262 243 144 117 109 108 54 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2229 4527 487 Size: 683 56 1003 2298 5057 Fragments arranged by size: 5057 2298 1003 683 56 BtsI GCAGTG_nn' Cuts at: 6109 6613 7407 7427 6109 Size: 504 794 20 7779 Fragments arranged by size: 7779 794 504 20 ClaI AT'CG_AT Cuts at: 1554 6324 1554 Size: 4770 4327 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9062 DraI TTT'AAA Cuts at: 6401 6401 Size: 9097 DraIII CAC_nnn'GTG Cuts at: 3719 3719 Size: 9097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5472 5562 8104 809 Size: 507 4156 90 2542 1802 Fragments arranged by size: 4156 2542 1802 507 90 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2068 2392 Size: 54 1425 50 275 18 216 22 324 Cuts at: 2392 4465 4645 5641 6022 6048 6544 7433 9062 Size: 2073 180 996 381 26 496 889 1629 Cuts at: 9062 8 Size: 43 Fragments arranged by size: 2073 1629 1425 996 889 496 381 324 275 216 180 54 50 43 26 22 18 EagI C'GGCC_G Cuts at: 1537 1812 6544 1537 Size: 275 4732 4090 Fragments arranged by size: 4732 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5807 7027 1353 Size: 4454 1220 3423 Fragments arranged by size: 4454 3423 1220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7684 8058 8204 387 Size: 1064 138 6095 374 146 1280 Fragments arranged by size: 6095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2894 3416 3857 4154 4388 2894 Size: 522 441 297 234 7603 Fragments arranged by size: 7603 522 441 297 234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2628 2658 2964 3114 3414 3792 3810 Size: 393 996 30 306 150 300 378 18 Cuts at: 3810 3873 3942 4017 4044 4166 4170 4404 4602 Size: 63 69 75 27 122 4 234 198 Cuts at: 4602 4989 5336 5421 5581 6158 7160 7757 8544 Size: 387 347 85 160 577 1002 597 787 Cuts at: 8544 8812 1239 Size: 268 1524 Fragments arranged by size: 1524 1002 996 787 597 577 393 387 378 347 306 300 268 234 198 160 150 122 85 75 69 63 30 27 18 4 EcoNI CCTnn'n_nnAGG Cuts at: 6158 6158 Size: 9097 EcoO109I rG'GnC_Cy Cuts at: 2339 2375 2384 2549 2612 2648 2657 2666 2675 Size: 36 9 165 63 36 9 9 9 Cuts at: 2675 2909 3000 3080 3081 3098 3251 3268 3269 Size: 234 91 80 1 17 153 17 1 Cuts at: 3269 3377 3521 3863 3908 4070 4223 4727 4961 Size: 108 144 342 45 162 153 504 234 Cuts at: 4961 5006 5276 5357 5393 5429 5493 6163 2339 Size: 45 270 81 36 36 64 670 5273 Fragments arranged by size: 5273 670 504 342 270 234 234 165 162 153 153 144 108 91 81 80 64 63 45 45 36 36 36 36 17 17 9 9 9 9 1 1 EcoRI G'AATT_C Cuts at: 6362 6406 6784 6362 Size: 44 378 8675 Fragments arranged by size: 8675 378 44 FspI TGC'GCA Cuts at: 7603 7603 Size: 9097 HaeII r_GCGC'y Cuts at: 1678 2256 3822 4011 4173 4608 5100 5253 5586 Size: 578 1566 189 162 435 492 153 333 Cuts at: 5586 8244 1678 Size: 2658 2531 Fragments arranged by size: 2658 2531 1566 578 492 435 333 189 162 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 2498 2530 4793 Size: 32 135 32 816 32 78 32 2263 Cuts at: 4793 4825 5182 5214 7738 7770 7812 7844 1373 Size: 32 357 32 2524 32 42 32 2626 Fragments arranged by size: 2626 2524 2263 816 357 135 78 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6441 6687 669 Size: 988 281 4503 246 3079 Fragments arranged by size: 4503 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9097 HpaI GTT'AAC Cuts at: 6687 6687 Size: 9097 KasI G'GCGC_C Cuts at: 2252 4007 4169 5096 5249 2252 Size: 1755 162 927 153 6100 Fragments arranged by size: 6100 1755 927 162 153 KpnI G_GTAC'C Cuts at: 2146 6422 2146 Size: 4276 4821 MfeI C'AATT_G Cuts at: 6696 6696 Size: 9097 MluI A'CGCG_T Cuts at: 8973 8973 Size: 9097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6057 6207 8211 8395 1305 Size: 4752 150 2004 184 2007 Fragments arranged by size: 4752 2007 2004 184 150 MscI TGG'CCA Cuts at: 10 64 1489 1832 6050 10 Size: 54 1425 343 4218 3057 Fragments arranged by size: 4218 3057 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5637 6326 7055 7414 7573 518 Size: 648 4471 689 729 359 159 2042 Fragments arranged by size: 4471 2042 729 689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2491 2780 3023 3303 3527 3887 4211 4365 Size: 885 289 243 280 224 360 324 154 Cuts at: 4365 4544 5129 6058 6513 7190 8338 8583 1606 Size: 179 585 929 455 677 1148 245 2120 Fragments arranged by size: 2120 1148 929 885 677 585 455 360 324 289 280 245 243 224 179 154 NarI GG'CG_CC Cuts at: 2253 4008 4170 5097 5250 2253 Size: 1755 162 927 153 6100 Fragments arranged by size: 6100 1755 927 162 153 NcoI C'CATG_G Cuts at: 513 1065 2544 2652 5001 513 Size: 552 1479 108 2349 4609 Fragments arranged by size: 4609 2349 1479 552 108 NdeI CA'TA_TG Cuts at: 387 387 Size: 9097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9097 NotI GC'GGCC_GC Cuts at: 6544 6544 Size: 9097 NspI r_CATG'y Cuts at: 1132 1229 5975 6455 8000 1132 Size: 97 4746 480 1545 2229 Fragments arranged by size: 4746 2229 1545 480 97 PasI CC'CwG_GG Cuts at: 1348 3103 3265 1348 Size: 1755 162 7180 Fragments arranged by size: 7180 1755 162 PciI A'CATG_T Cuts at: 7996 7996 Size: 9097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9097 PfoI T'CCnGG_A Cuts at: 2425 2452 3805 3994 4579 5551 2425 Size: 27 1353 189 585 972 5971 Fragments arranged by size: 5971 1353 972 585 189 27 PmeI GTTT'AAAC Cuts at: 6401 6401 Size: 9097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7166 7198 8679 8711 7166 Size: 32 1481 32 7552 Fragments arranged by size: 7552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2339 2375 2549 2612 2648 2657 2666 2675 3098 Size: 36 174 63 36 9 9 9 423 Cuts at: 3098 3377 3521 3863 4070 4727 4961 5357 5393 Size: 279 144 342 207 657 234 396 36 Cuts at: 5393 5429 2339 Size: 36 6007 Fragments arranged by size: 6007 657 423 396 342 279 234 207 174 144 63 36 36 36 36 9 9 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9097 PsiI TTA'TAA Cuts at: 6667 6667 Size: 9097 PspOMI G'GGCC_C Cuts at: 2908 2999 3080 3268 5005 5492 2908 Size: 91 81 188 1737 487 6513 Fragments arranged by size: 6513 1737 487 188 91 81 PspXI vC'TCGA_Gb Cuts at: 2760 3417 5690 2760 Size: 657 2273 6167 Fragments arranged by size: 6167 2273 657 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6089 6121 6089 Size: 32 9065 PstI C_TGCA'G Cuts at: 830 6449 830 Size: 5619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9097 PvuII CAG'CTG Cuts at: 2491 2780 4211 6058 2491 Size: 289 1431 1847 5530 Fragments arranged by size: 5530 1847 1431 289 SacI G_AGCT'C Cuts at: 2896 3418 3859 4156 4390 2896 Size: 522 441 297 234 7603 Fragments arranged by size: 7603 522 441 297 234 SalI G'TCGA_C Cuts at: 1936 6439 1936 Size: 4503 4594 SanDI GG'GwC_CC Cuts at: 2339 2375 2549 2657 3521 3863 5357 2339 Size: 36 174 108 864 342 1494 6079 Fragments arranged by size: 6079 1494 864 342 174 108 36 SbfI CC_TGCA'GG Cuts at: 6449 6449 Size: 9097 ScaI AGT'ACT Cuts at: 5825 7345 9057 5825 Size: 1520 1712 5865 Fragments arranged by size: 5865 1712 1520 SexAI A'CCwGG_T Cuts at: 2590 2716 3310 3472 3715 3976 4471 4489 2590 Size: 126 594 162 243 261 495 18 7198 Fragments arranged by size: 7198 594 495 261 243 162 126 18 SfcI C'TryA_G Cuts at: 826 1045 6226 6445 7580 8261 8452 826 Size: 219 5181 219 1135 681 191 1471 Fragments arranged by size: 5181 1471 1135 681 219 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2364 2391 2746 2916 3132 3276 3555 5175 2364 Size: 27 355 170 216 144 279 1620 6286 Fragments arranged by size: 6286 1620 355 279 216 170 144 27 SfoI GGC'GCC Cuts at: 2254 4009 4171 5098 5251 2254 Size: 1755 162 927 153 6100 Fragments arranged by size: 6100 1755 927 162 153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9097 SgrAI Cr'CCGG_yG Cuts at: 1700 2327 1700 Size: 627 8470 SmaI CCC'GGG Cuts at: 1872 4852 6424 1872 Size: 2980 1572 4545 Fragments arranged by size: 4545 2980 1572 SmlI C'TyrA_G Cuts at: 820 1017 1943 2760 3417 4857 5619 5690 5708 Size: 197 926 817 657 1440 762 71 18 Cuts at: 5708 5789 6155 7201 8102 8364 820 Size: 81 366 1046 901 262 1553 Fragments arranged by size: 1553 1440 1046 926 901 817 762 657 366 262 197 81 71 18 SnaBI TAC'GTA Cuts at: 493 6888 493 Size: 6395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9097 SphI G_CATG'C Cuts at: 1132 6455 1132 Size: 5323 3774 SspI AAT'ATT Cuts at: 5 52 7021 5 Size: 47 6969 2081 Fragments arranged by size: 6969 2081 47 StuI AGG'CCT Cuts at: 1517 6089 1517 Size: 4572 4525 StyI C'CwwG_G Cuts at: 513 1065 2343 2369 2544 2599 2608 2652 2832 Size: 552 1278 26 175 55 9 44 180 Cuts at: 2832 3076 3291 3516 3615 4003 5001 5100 5272 Size: 244 215 225 99 388 998 99 172 Cuts at: 5272 5842 5958 6336 6536 513 Size: 570 116 378 200 3074 Fragments arranged by size: 3074 1278 998 570 552 388 378 244 225 215 200 180 175 172 116 99 99 55 44 26 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2166 2323 3815 4643 6011 7124 7141 Size: 1255 586 157 1492 828 1368 1113 17 Cuts at: 7141 7294 7479 7924 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1492 1368 1255 1113 828 586 445 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5823 7343 9055 Size: 274 80 33 51 1443 3846 1520 1712 Cuts at: 9055 96 Size: 138 Fragments arranged by size: 3846 1712 1520 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2517 2549 9057 9089 2517 Size: 32 6508 32 2525 Fragments arranged by size: 6508 2525 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9097 XbaI T'CTAG_A Cuts at: 6433 6433 Size: 9097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2495 2615 3110 3938 4172 5279 5360 6014 6325 Size: 120 495 828 234 1107 81 654 311 Cuts at: 6325 2495 Size: 5267 Fragments arranged by size: 5267 1107 828 654 495 311 234 120 81 XhoI C'TCGA_G Cuts at: 1943 2760 3417 5690 1943 Size: 817 657 2273 5350 Fragments arranged by size: 5350 2273 817 657 XmaI C'CCGG_G Cuts at: 1870 4850 6422 1870 Size: 2980 1572 4545 Fragments arranged by size: 4545 2980 1572 XmnI GAAnn'nnTTC Cuts at: 7226 7226 Size: 9097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4757 6905 276 Size: 53 83 186 936 3223 2148 2468 Fragments arranged by size: 3223 2468 2148 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI RsrII SacII SapI SrfI SwaI