(Circular) (Six-base) MAPSORT of: pFN21AB4626.seq Check: 5532 from: 1 to: 7015 pFN21AB4626 7015 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4228 4228 Size: 7015 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4825 278 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4358 1937 Size: 2421 4594 Acc65I G'GTAC_C Cuts at: 4336 4336 Size: 7015 AclI AA'CG_TT Cuts at: 1563 5142 5515 1563 Size: 3579 373 3063 Fragments arranged by size: 3579 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3338 3599 5078 6462 6730 1632 Size: 1706 261 1479 1384 268 1917 Fragments arranged by size: 1917 1706 1479 1384 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6818 AflIII A'CryG_T Cuts at: 1203 2447 2877 5914 6891 1203 Size: 1244 430 3037 977 1327 Fragments arranged by size: 3037 1327 1244 977 430 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 5744 5744 Size: 7015 AleI CACnn'nnGTG Cuts at: 1166 2945 3804 1166 Size: 1779 859 4377 Fragments arranged by size: 4377 1779 859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2102 2220 2541 2981 3696 1184 Size: 918 118 321 440 715 4503 Fragments arranged by size: 4503 918 715 440 321 118 AlwNI CAG_nnn'CTG Cuts at: 2169 2344 2545 4006 4228 4758 6330 2169 Size: 175 201 1461 222 530 1572 2854 Fragments arranged by size: 2854 1572 1461 530 222 201 175 ApaI G_GGCC'C Cuts at: 3256 3596 3256 Size: 340 6675 ApaLI G'TGCA_C Cuts at: 2114 2195 2354 3671 5072 6228 2114 Size: 81 159 1317 1401 1156 2901 Fragments arranged by size: 2901 1401 1317 1156 159 81 ApoI r'AATT_y Cuts at: 1493 4324 4550 4702 6811 1493 Size: 2831 226 152 2109 1697 Fragments arranged by size: 2831 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 2419 3042 3131 4299 4340 1870 Size: 73 476 623 89 1168 41 4545 Fragments arranged by size: 4545 1168 623 476 89 73 41 BamHI G'GATC_C Cuts at: 3696 4345 4712 3696 Size: 649 367 5999 Fragments arranged by size: 5999 649 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 2504 2637 3233 3463 Size: 325 217 627 684 33 133 596 230 Cuts at: 3463 3511 4205 4336 5791 618 Size: 48 694 131 1455 1842 Fragments arranged by size: 1842 1455 694 684 627 596 325 230 217 133 131 48 33 BanII G_rGCy'C Cuts at: 1391 2682 3219 3256 3393 3596 3612 4133 4203 Size: 1291 537 37 137 203 16 521 70 Cuts at: 4203 4334 1391 Size: 131 4072 Fragments arranged by size: 4072 1291 537 521 203 137 131 70 37 16 BbeI G_GCGC'C Cuts at: 3237 3515 4209 3237 Size: 278 694 6043 Fragments arranged by size: 6043 694 278 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3903 3980 4190 928 Size: 971 2004 77 210 3753 Fragments arranged by size: 3753 2004 971 210 77 BbvCI CC'TCA_GC Cuts at: 2402 3718 2402 Size: 1316 5699 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5206 5240 1903 Size: 34 3269 34 3678 Fragments arranged by size: 3678 3269 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4907 6117 4907 Size: 1210 5805 BclI T'GATC_A Cuts at: 1548 3060 1548 Size: 1512 5503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2630 3457 5626 Size: 107 122 71 631 605 958 827 2169 Cuts at: 5626 136 Size: 1525 Fragments arranged by size: 2169 1525 958 827 631 605 122 107 71 BglII A'GATC_T Cuts at: 2765 7010 2765 Size: 4245 2770 BlpI GC'TnA_GC Cuts at: 2843 4432 2843 Size: 1589 5426 Bme1580I G_kGCm'C Cuts at: 1790 2118 2199 2358 2476 2507 2640 2970 3256 Size: 328 81 159 118 31 133 330 286 Cuts at: 3256 3468 3596 3675 3729 4050 4194 5076 6232 Size: 212 128 79 54 321 144 882 1156 Cuts at: 6232 1790 Size: 2573 Fragments arranged by size: 2573 1156 882 330 328 321 286 212 159 144 133 128 118 81 79 54 31 BmgBI CAC'GTC Cuts at: 1318 2878 3676 1318 Size: 1560 798 4657 Fragments arranged by size: 4657 1560 798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4205 4746 Size: 416 53 83 399 24 2060 728 541 Cuts at: 4746 5704 442 Size: 958 1753 Fragments arranged by size: 2060 1753 958 728 541 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4105 4105 Size: 7015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 3332 3438 4136 5675 1239 Size: 1223 870 106 698 1539 2579 Fragments arranged by size: 2579 1539 1223 870 698 106 Bpu10I CC'TnA_GC Cuts at: 2402 3110 3380 3718 2402 Size: 708 270 338 5699 Fragments arranged by size: 5699 708 338 270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5140 6005 6303 5140 Size: 865 298 5852 Fragments arranged by size: 5852 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3924 5678 882 Size: 617 2425 1754 2219 Fragments arranged by size: 2425 2219 1754 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 4806 493 Size: 711 1317 2285 2702 Fragments arranged by size: 2702 2285 1317 711 BsaBI GATnn'nnATC Cuts at: 7009 7009 Size: 7015 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2131 3234 3512 3657 Size: 53 83 186 936 598 1103 278 145 Cuts at: 3657 4206 4822 5204 5839 275 Size: 549 616 382 635 1451 Fragments arranged by size: 1451 1103 936 635 616 598 549 382 278 186 145 83 53 BsaWI w'CCGG_w Cuts at: 2118 3393 3693 3699 5448 5846 6120 6267 6678 Size: 1275 300 6 1749 398 274 147 411 Cuts at: 6678 2118 Size: 2455 Fragments arranged by size: 2455 1749 1275 411 398 300 274 147 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2570 2600 3139 3169 3462 Size: 30 165 30 1167 30 539 30 293 Cuts at: 3462 3492 1178 Size: 30 4701 Fragments arranged by size: 4701 1167 539 293 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3259 3730 1186 Size: 2073 471 4471 Fragments arranged by size: 4471 2073 471 BseYI C'CCAG_C Cuts at: 2064 3853 3883 3950 6218 6714 2064 Size: 1789 30 67 2268 496 2365 Fragments arranged by size: 2365 2268 1789 496 67 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2501 3371 1163 Size: 573 546 219 870 4807 Fragments arranged by size: 4807 870 573 546 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2272 4465 5226 6254 1540 Size: 275 186 271 2193 761 1028 2301 Fragments arranged by size: 2301 2193 1028 761 275 271 186 BsiHKAI G_wGCw'C Cuts at: 2118 2199 2238 2358 2682 3393 3527 3612 3675 Size: 81 39 120 324 711 134 85 63 Cuts at: 3675 4133 5076 5161 6232 2118 Size: 458 943 85 1071 2901 Fragments arranged by size: 2901 1071 943 711 458 324 134 120 85 85 81 63 39 BsmI GAATG_Cn' Cuts at: 4526 4619 4526 Size: 93 6922 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2199 2238 2358 2476 2507 2640 Size: 399 328 81 39 120 118 31 133 Cuts at: 2640 2682 2970 3219 3256 3393 3468 3527 3596 Size: 42 288 249 37 137 75 59 69 Cuts at: 3596 3612 3675 3729 4050 4133 4194 4203 4334 Size: 16 63 54 321 83 61 9 131 Cuts at: 4334 5076 5161 6232 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 321 288 249 137 133 131 120 118 85 83 81 75 69 63 61 59 54 42 39 37 31 16 9 BspEI T'CCGG_A Cuts at: 3393 3699 6678 3393 Size: 306 2979 3730 Fragments arranged by size: 3730 2979 306 BspHI T'CATG_A Cuts at: 3360 4904 6634 3360 Size: 1544 1730 3741 Fragments arranged by size: 3741 1730 1544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3444 3973 4228 4303 4370 844 Size: 2600 529 255 75 67 3489 Fragments arranged by size: 3489 2600 529 255 75 67 BsrBI CCG'CTC Cuts at: 1394 1823 2027 2268 4902 1394 Size: 429 204 241 2634 3507 Fragments arranged by size: 3507 2634 429 241 204 BsrDI GCAATG_nn' Cuts at: 65 1248 5510 5684 65 Size: 1183 4262 174 1396 Fragments arranged by size: 4262 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2271 2688 2850 3230 3460 4161 5659 Size: 110 461 417 162 380 230 701 1498 Cuts at: 5659 5846 1700 Size: 187 2869 Fragments arranged by size: 2869 1498 701 461 417 380 230 187 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 3781 5075 6087 3781 Size: 1294 1012 4709 Fragments arranged by size: 4709 1294 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2194 2242 794 Size: 1400 48 5567 Fragments arranged by size: 5567 1400 48 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 3985 4285 1184 Size: 2801 300 3914 Fragments arranged by size: 3914 2801 300 BstXI CCAn_nnnn'nTGG Cuts at: 3806 4282 3806 Size: 476 6539 BstYI r'GATC_y Cuts at: 1972 2765 3411 3696 3937 4062 4345 4712 5097 Size: 793 646 285 241 125 283 367 385 Cuts at: 5097 5114 6566 6652 6664 7010 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 793 646 385 367 346 285 283 241 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2871 3507 3799 513 Size: 552 1806 636 292 3729 Fragments arranged by size: 3729 1806 636 552 292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4246 487 Size: 683 56 3020 3256 Fragments arranged by size: 3256 3020 683 56 BtsI GCAGTG_nn' Cuts at: 2543 2919 2965 3811 3848 4133 4531 5325 5345 Size: 376 46 846 37 285 398 794 20 Cuts at: 5345 2543 Size: 4213 Fragments arranged by size: 4213 846 794 398 376 285 46 37 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 4319 4319 Size: 7015 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6022 809 Size: 507 4706 1802 Fragments arranged by size: 4706 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2269 2602 2811 Size: 54 1425 50 275 18 439 333 209 Cuts at: 2811 4462 5351 6980 8 Size: 1651 889 1629 43 Fragments arranged by size: 1651 1629 1425 889 439 333 275 209 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2269 4462 1537 Size: 275 457 2193 4090 Fragments arranged by size: 4090 2193 457 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2893 3863 4100 4945 1353 Size: 1540 970 237 845 3423 Fragments arranged by size: 3423 1540 970 845 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2666 3443 3509 5602 5976 6122 Size: 1064 138 1077 777 66 2093 374 146 Cuts at: 6122 387 Size: 1280 Fragments arranged by size: 2093 1280 1077 1064 777 374 146 138 66 EcoICRI GAG'CTC Cuts at: 2680 3391 3610 4131 2680 Size: 711 219 521 5564 Fragments arranged by size: 5564 711 521 219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 3332 3338 3438 3599 4136 5078 Size: 393 830 870 6 100 161 537 942 Cuts at: 5078 5675 6462 6730 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 942 870 830 787 597 537 393 268 161 100 6 EcoO109I rG'GnC_Cy Cuts at: 3325 3592 3593 3844 3325 Size: 267 1 251 6496 Fragments arranged by size: 6496 267 251 1 EcoRI G'AATT_C Cuts at: 4324 4702 4324 Size: 378 6637 FspI TGC'GCA Cuts at: 3224 5521 3224 Size: 2297 4718 HaeII r_GCGC'y Cuts at: 1678 2052 2169 2843 3237 3515 4158 4209 6162 Size: 374 117 674 394 278 643 51 1953 Cuts at: 6162 1678 Size: 2531 Fragments arranged by size: 2531 1953 674 643 394 374 278 117 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 2570 2602 5656 Size: 32 135 32 549 32 417 32 3054 Cuts at: 5656 5688 5730 5762 1373 Size: 32 42 32 2626 Fragments arranged by size: 3054 2626 549 417 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2983 3370 4359 4605 669 Size: 988 281 1045 387 989 246 3079 Fragments arranged by size: 3079 1045 989 988 387 281 246 HindIII A'AGCT_T Cuts at: 748 2360 3788 748 Size: 1612 1428 3975 Fragments arranged by size: 3975 1612 1428 HpaI GTT'AAC Cuts at: 2983 4605 2983 Size: 1622 5393 KasI G'GCGC_C Cuts at: 3233 3511 4205 3233 Size: 278 694 6043 Fragments arranged by size: 6043 694 278 KpnI G_GTAC'C Cuts at: 4340 4340 Size: 7015 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6129 6313 1305 Size: 4824 184 2007 Fragments arranged by size: 4824 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2813 10 Size: 54 1425 343 981 4212 Fragments arranged by size: 4212 1425 981 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 2876 2945 3506 3804 4973 5332 Size: 648 1564 146 69 561 298 1169 359 Cuts at: 5332 5491 518 Size: 159 2042 Fragments arranged by size: 2042 1564 1169 648 561 359 298 159 146 69 MspA1I CmG'CkG Cuts at: 1606 3722 3748 4225 4282 4431 5108 6256 6501 Size: 2116 26 477 57 149 677 1148 245 Cuts at: 6501 1606 Size: 2120 Fragments arranged by size: 2120 2116 1148 677 477 245 149 57 26 NaeI GCC'GGC Cuts at: 2273 2273 Size: 7015 NarI GG'CG_CC Cuts at: 3234 3512 4206 3234 Size: 278 694 6043 Fragments arranged by size: 6043 694 278 NcoI C'CATG_G Cuts at: 513 1065 2871 3507 513 Size: 552 1806 636 4021 Fragments arranged by size: 4021 1806 636 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7015 NgoMIV G'CCGG_C Cuts at: 2271 2271 Size: 7015 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NspI r_CATG'y Cuts at: 1132 1229 2451 2785 4373 5918 1132 Size: 97 1222 334 1588 1545 2229 Fragments arranged by size: 2229 1588 1545 1222 334 97 PasI CC'CwG_GG Cuts at: 1348 2467 3599 1348 Size: 1119 1132 4764 Fragments arranged by size: 4764 1132 1119 PciI A'CATG_T Cuts at: 2447 5914 2447 Size: 3467 3548 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 2647 1108 Size: 1236 303 5476 Fragments arranged by size: 5476 1236 303 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PmlI CAC'GTG Cuts at: 2521 2521 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5084 5116 6597 6629 5084 Size: 32 1481 32 5470 Fragments arranged by size: 5470 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3844 3844 Size: 7015 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7015 PsiI TTA'TAA Cuts at: 4585 4585 Size: 7015 PspOMI G'GGCC_C Cuts at: 3252 3592 3252 Size: 340 6675 PspXI vC'TCGA_Gb Cuts at: 2419 3131 2419 Size: 712 6303 PstI C_TGCA'G Cuts at: 830 2048 2111 2190 2550 2838 3303 3405 3426 Size: 1218 63 79 360 288 465 102 21 Cuts at: 3426 3441 3627 4170 4367 830 Size: 15 186 543 197 3478 Fragments arranged by size: 3478 1218 543 465 360 288 197 186 102 79 63 21 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 3722 4225 4282 3722 Size: 503 57 6455 Fragments arranged by size: 6455 503 57 SacI G_AGCT'C Cuts at: 2682 3393 3612 4133 2682 Size: 711 219 521 5564 Fragments arranged by size: 5564 711 521 219 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SanDI GG'GwC_CC Cuts at: 3844 3844 Size: 7015 SbfI CC_TGCA'GG Cuts at: 4367 4367 Size: 7015 ScaI AGT'ACT Cuts at: 5263 6975 5263 Size: 1712 5303 SfcI C'TryA_G Cuts at: 826 1045 2044 2107 2186 2546 2834 3299 3401 Size: 219 999 63 79 360 288 465 102 Cuts at: 3401 3422 3437 3623 4166 4363 5498 6179 6370 Size: 21 15 186 543 197 1135 681 191 Cuts at: 6370 826 Size: 1471 Fragments arranged by size: 1471 1135 999 681 543 465 360 288 219 197 191 186 102 79 63 21 15 SfoI GGC'GCC Cuts at: 3235 3513 4207 3235 Size: 278 694 6043 Fragments arranged by size: 6043 694 278 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 3230 1700 Size: 1530 5485 SmaI CCC'GGG Cuts at: 1872 4342 1872 Size: 2470 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 3131 5119 6020 6282 820 Size: 197 926 476 712 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 712 476 262 197 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 4373 1132 Size: 3241 3774 SspI AAT'ATT Cuts at: 5 52 4939 5 Size: 47 4887 2081 Fragments arranged by size: 4887 2081 47 StuI AGG'CCT Cuts at: 1517 3548 1517 Size: 2031 4984 StyI C'CwwG_G Cuts at: 513 1065 2529 2871 3507 4266 4454 513 Size: 552 1464 342 636 759 188 3074 Fragments arranged by size: 3074 1464 759 636 552 342 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 2508 2626 3520 3921 4094 5042 Size: 1255 879 49 118 894 401 173 948 Cuts at: 5042 5059 5212 5397 5842 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 948 894 879 445 401 185 173 153 118 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5261 6973 96 Size: 274 80 33 51 1443 3284 1712 138 Fragments arranged by size: 3284 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3139 3171 3462 3494 6975 7007 3139 Size: 32 291 32 3481 32 3147 Fragments arranged by size: 3481 3147 291 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2297 1316 Size: 981 6034 XbaI T'CTAG_A Cuts at: 3877 4351 3877 Size: 474 6541 XhoI C'TCGA_G Cuts at: 1943 2419 3131 1943 Size: 476 712 5827 Fragments arranged by size: 5827 712 476 XmaI C'CCGG_G Cuts at: 1870 4340 1870 Size: 2470 4545 XmnI GAAnn'nnTTC Cuts at: 5144 5144 Size: 7015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4823 276 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI