Restriction map for
pFN21AB4249
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BseRI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI CpoI
DraI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MseI MunI MvnI NarI NciI
NdeI NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 720 GGCGTC G[GA]^CG[CT]C
Alw44I 916 GTGCAC G^TGCAC
AlwI 3 GGATC GGATC(4/5)
ApaLI 916 GTGCAC G^TGCAC
Asp700I 981 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 601 CCCGAG C^[CT]CG[GA]G
BglI 712 GCCATCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 720 GGCGTC G[GA]^CG[CT]C
BsmBI 1051 GAGACG CGTCTC(1/5)
BsmFI 101 GTCCC GGGAC(10/14)
BsoBI 601 CCCGAG C^[CT]CG[GA]G
BspDI 1030 ATCGAT AT^CGAT
BspMI 856 ACCTGC ACCTGC(4/8)
BsrDI 261 CATTGC GCAATG(2/0)
BssSI 310 CTCGTG CACGAG(-5/-1)
BstAPI 68 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 182 CCAACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3 GGATCT [GA]^GATC[CT]
ClaI 1030 ATCGAT AT^CGAT
DraIII 897 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 552 GACCGTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 720 GGCGTC G[GA]^CG[CT]C
HincII 928 GTCAAC GT[CT]^[GA]AC
HindII 928 GTCAAC GT[CT]^[GA]AC
HinfI 1043 GAGTC G^A[ACGT]TC
MamI 4 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3 GGATCT [GA]^GATC[CT]
MspA1I 51 CAGCTG C[AC]G^C[GT]G
NaeI 860 GCCGGC GCC^GGC
NcoI 332 CCATGG C^CATGG
NgoMIV 860 GCCGGC G^CCGGC
PleI 1043 GAGTC GAGTC(4/5)
PvuII 51 CAGCTG CAG^CTG
SacI 340 GAGCTC GAGCT^C
StuI 983 AGGCCT AGG^CCT
XcmI 182 CCAACTCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3 GGATCT [GA]^GATC[CT]
XmnI 981 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 423 CCGC CCGC(-3/-1)
811 GCGG
AlwNI 69 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
901 CAGTGCCTG
AvaII 420 GGACC G^G[AT]CC
590 GGTCC
BanII 340 GAGCTC G[GA]GC[CT]^C
1015 GGGCTC
BpmI 1038 CTCCAG CTGGAG(16/14)
865 CTGGAG
BsaI 577 GAGACC GGTCTC(1/5)
642 GGTCTC
BsgI 838 CTGCAC GTGCAG(16/14)
313 GTGCAG
BsrFI 587 GCCGGT [GA]^CCGG[CT]
860 GCCGGC
Cfr10I 587 GCCGGT [GA]^CCGG[CT]
860 GCCGGC
DdeI 24 CTCAG C^T[ACGT]AG
1047 CTCAG
DsaI 188 CCGTGG C^C[GA][CT]GG
332 CCATGG
EcoT14I 332 CCATGG C^C[AT][AT]GG
1067 CCTTGG
NspI 107 ACATGC [GA]CATG^[CT]
891 GCATGT
ScaI 998 AGTACT AGT^ACT
1058 AGTACT
SfcI 942 CTACAG C^T[GA][CT]AG
963 CTACAG
StyI 332 CCATGG C^C[AT][AT]GG
1067 CCTTGG
TaqI 17 TCGA T^CGA
1031 TCGA
TthHB8I 17 TCGA T^CGA
1031 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AfaI AluI AspHI BalI BbrPI
BbsI BbvI BmyI BpuAI BsaAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EaeI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NdeII NlaIII PmaCI PmlI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI