Restriction map for
pFN21AB4249
(Linear) (Six-base) MAPSORT of: KIBB4249.seq Check: 3052 from: 1 to: 1086
KIBB4249.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 865 1086
Size: 865 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1086
Size: 568 518
AlwNI CAG_nnn'CTG
Cuts at: 0 74 906 1086
Size: 74 832 180
Fragments arranged by size:
832 180 74
ApaLI G'TGCA_C
Cuts at: 0 916 1086
Size: 916 170
AvaI C'yCGr_G
Cuts at: 0 601 1086
Size: 601 485
BanII G_rGCy'C
Cuts at: 0 344 1019 1086
Size: 344 675 67
Fragments arranged by size:
675 344 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 681 771 1086
Size: 556 125 90 315
Fragments arranged by size:
556 315 125 90
BglI GCCn_nnn'nGGC
Cuts at: 0 718 1086
Size: 718 368
Bme1580I G_kGCm'C
Cuts at: 0 920 1086
Size: 920 166
BmgBI CAC'GTC
Cuts at: 0 560 1086
Size: 560 526
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1086
Size: 286 800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 660 1086
Size: 660 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1023 1086
Size: 886 137 63
Fragments arranged by size:
886 137 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 571 648 1086
Size: 571 77 438
Fragments arranged by size:
571 438 77
BsaAI yAC'GTr
Cuts at: 0 347 407 771 807 1086
Size: 347 60 364 36 279
Fragments arranged by size:
364 347 279 60 36
BsaBI GATnn'nnATC
Cuts at: 0 8 1086
Size: 8 1078
BsaHI Gr'CG_yC
Cuts at: 0 721 1086
Size: 721 365
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 814 844 1086
Size: 124 30 660 30 242
Fragments arranged by size:
660 242 124 30 30
BseYI C'CCAG_C
Cuts at: 0 457 1086
Size: 457 629
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 823 1086
Size: 334 489 263
Fragments arranged by size:
489 334 263
BsiHKAI G_wGCw'C
Cuts at: 0 344 608 872 920 1081 1086
Size: 344 264 264 48 161 5
Fragments arranged by size:
344 264 264 161 48 5
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1045 1086
Size: 1045 41
Bsp1286I G_dGCh'C
Cuts at: 0 344 608 872 920 1019 1081 1086
Size: 344 264 264 48 99 62 5
Fragments arranged by size:
344 264 264 99 62 48 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 865 1086
Size: 865 221
BsrDI GCAATG_nn'
Cuts at: 0 260 1086
Size: 260 826
BsrFI r'CCGG_y
Cuts at: 0 587 860 1086
Size: 587 273 226
Fragments arranged by size:
587 273 226
BssSI C'ACGA_G
Cuts at: 0 310 1086
Size: 310 776
BstAPI GCAn_nnn'nTGC
Cuts at: 0 74 1086
Size: 74 1012
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 1086
Size: 189 897
BstYI r'GATC_y
Cuts at: 0 3 1086
Size: 3 1083
BtgI C'CryG_G
Cuts at: 0 188 332 1086
Size: 188 144 754
Fragments arranged by size:
754 188 144
BtsI GCAGTG_nn'
Cuts at: 0 237 918 1086
Size: 237 681 168
Fragments arranged by size:
681 237 168
ClaI AT'CG_AT
Cuts at: 0 1031 1086
Size: 1031 55
DraIII CAC_nnn'GTG
Cuts at: 0 902 1086
Size: 902 184
DrdI GACnn_nn'nnGTC
Cuts at: 0 558 1086
Size: 558 528
EaeI y'GGCC_r
Cuts at: 0 55 257 704 1004 1086
Size: 55 202 447 300 82
Fragments arranged by size:
447 300 202 82 55
EarI CTCTTCn'nnn_
Cuts at: 0 19 388 883 1086
Size: 19 369 495 203
Fragments arranged by size:
495 369 203 19
EcoICRI GAG'CTC
Cuts at: 0 342 1086
Size: 342 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 886 1023 1086
Size: 568 318 137 63
Fragments arranged by size:
568 318 137 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 777 1086
Size: 777 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 1086
Size: 814 32 240
Fragments arranged by size:
814 240 32
HincII GTy'rAC
Cuts at: 0 930 1086
Size: 930 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1086
Size: 593 493
MscI TGG'CCA
Cuts at: 0 57 259 706 1086
Size: 57 202 447 380
Fragments arranged by size:
447 380 202 57
MslI CAynn'nnrTG
Cuts at: 0 82 94 172 409 415 499 1086
Size: 82 12 78 237 6 84 587
Fragments arranged by size:
587 237 84 82 78 12 6
MspA1I CmG'CkG
Cuts at: 0 53 1086
Size: 53 1033
NaeI GCC'GGC
Cuts at: 0 862 1086
Size: 862 224
NcoI C'CATG_G
Cuts at: 0 332 1086
Size: 332 754
NgoMIV G'CCGG_C
Cuts at: 0 860 1086
Size: 860 226
NspI r_CATG'y
Cuts at: 0 111 895 1086
Size: 111 784 191
Fragments arranged by size:
784 191 111
PmlI CAC'GTG
Cuts at: 0 347 407 807 1086
Size: 347 60 400 279
Fragments arranged by size:
400 347 279 60
PvuII CAG'CTG
Cuts at: 0 53 1086
Size: 53 1033
SacI G_AGCT'C
Cuts at: 0 344 1086
Size: 344 742
ScaI AGT'ACT
Cuts at: 0 1000 1060 1086
Size: 1000 60 26
Fragments arranged by size:
1000 60 26
SfcI C'TryA_G
Cuts at: 0 942 963 1086
Size: 942 21 123
Fragments arranged by size:
942 123 21
StuI AGG'CCT
Cuts at: 0 985 1086
Size: 985 101
StyI C'CwwG_G
Cuts at: 0 332 1067 1086
Size: 332 735 19
Fragments arranged by size:
735 332 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 288 1086
Size: 189 99 798
Fragments arranged by size:
798 189 99
TatI w'GTAC_w
Cuts at: 0 998 1058 1086
Size: 998 60 28
Fragments arranged by size:
998 60 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 1086
Size: 189 897
XmnI GAAnn'nnTTC
Cuts at: 0 985 1086
Size: 985 101
Enzymes that do cut:
AarI AcuI AlwNI ApaLI AvaI BanII BbsI BglI
Bme1580I BmgBI BmrI BplI BpmI BsaI BsaAI BsaBI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI
ClaI DraIII DrdI EaeI EarI EcoICRI Eco57MI FalI
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PmlI PvuII SacI ScaI SfcI StuI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI Bpu10I BpuEI
BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI