(Circular) (Six-base) MAPSORT of: pFN21AB4066.seq Check: 6253 from: 1 to: 5977 pFN21AB4066 5977 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3125 3298 3125 Size: 173 5804 AclI AA'CG_TT Cuts at: 1563 2049 4104 4477 1563 Size: 486 2055 373 3063 Fragments arranged by size: 3063 2055 486 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 4040 5424 5692 1632 Size: 602 1806 1384 268 1917 Fragments arranged by size: 1917 1806 1384 602 268 AflII C'TTAA_G Cuts at: 820 1017 2953 820 Size: 197 1936 3844 Fragments arranged by size: 3844 1936 197 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2167 3720 5292 2167 Size: 1553 1572 2852 Fragments arranged by size: 2852 1572 1553 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2695 2738 2993 3078 3286 3512 3664 5773 Size: 1202 43 255 85 208 226 152 2109 Cuts at: 5773 1493 Size: 1697 Fragments arranged by size: 2109 1697 1202 255 226 208 152 85 43 AseI AT'TA_AT Cuts at: 160 1032 3168 4531 160 Size: 872 2136 1363 1606 Fragments arranged by size: 2136 1606 1363 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3302 1870 Size: 73 1359 4545 Fragments arranged by size: 4545 1359 73 BamHI G'GATC_C Cuts at: 2662 3307 3674 2662 Size: 645 367 4965 Fragments arranged by size: 4965 645 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3125 3298 4753 618 Size: 325 217 627 1338 173 1455 1842 Fragments arranged by size: 1842 1455 1338 627 325 217 173 BanII G_rGCy'C Cuts at: 1391 2142 2198 3296 1391 Size: 751 56 1098 4072 Fragments arranged by size: 4072 1098 751 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4168 4202 1903 Size: 34 50 34 2147 34 3678 Fragments arranged by size: 3678 2147 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2646 4588 136 Size: 107 122 71 631 605 974 1942 1525 Fragments arranged by size: 1942 1525 974 631 605 122 107 71 BglII A'GATC_T Cuts at: 2905 5972 2905 Size: 3067 2910 BlpI GC'TnA_GC Cuts at: 2759 3394 2759 Size: 635 5342 Bme1580I G_kGCm'C Cuts at: 1790 4038 5194 1790 Size: 2248 1156 2573 Fragments arranged by size: 2573 2248 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3261 3708 4666 Size: 416 53 83 399 24 1844 447 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1844 1753 958 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2006 2147 2879 4637 1239 Size: 767 141 732 1758 2579 Fragments arranged by size: 2579 1758 767 732 141 Bpu10I CC'TnA_GC Cuts at: 2259 2349 2259 Size: 90 5887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2460 4102 4967 5265 2460 Size: 1642 865 298 3172 Fragments arranged by size: 3172 1642 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2122 2368 2846 4640 882 Size: 617 623 246 478 1794 2219 Fragments arranged by size: 2219 1794 623 617 478 246 BsaAI yAC'GTr Cuts at: 493 1204 2067 3768 493 Size: 711 863 1701 2702 Fragments arranged by size: 2702 1701 863 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2305 4410 4808 5082 5229 5640 2305 Size: 2105 398 274 147 411 2642 Fragments arranged by size: 2642 2105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 1186 Size: 964 5013 BseYI C'CCAG_C Cuts at: 2862 5180 5676 2862 Size: 2318 496 3163 Fragments arranged by size: 3163 2318 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 1163 Size: 573 693 4711 Fragments arranged by size: 4711 693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2198 3296 4038 4123 5194 1391 Size: 399 352 56 1098 742 85 1071 2174 Fragments arranged by size: 2174 1098 1071 742 399 352 85 56 BspEI T'CCGG_A Cuts at: 2305 5640 2305 Size: 3335 2642 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3189 4472 4646 65 Size: 1183 1941 1283 174 1396 Fragments arranged by size: 1941 1396 1283 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 2551 2824 96 Size: 2455 273 3249 Fragments arranged by size: 3249 2455 273 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2755 3243 794 Size: 1961 488 3528 Fragments arranged by size: 3528 1961 488 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2662 2905 3307 3674 4059 4076 5528 5614 Size: 690 243 402 367 385 17 1452 86 Cuts at: 5614 5626 5972 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 690 402 385 367 346 243 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2201 2599 513 Size: 552 1136 398 3891 Fragments arranged by size: 3891 1136 552 398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 3254 3493 4287 4307 3254 Size: 239 794 20 4924 Fragments arranged by size: 4924 794 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942 Size: 54 1425 50 275 18 1594 889 1629 Cuts at: 5942 8 Size: 43 Fragments arranged by size: 1629 1594 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 3907 1353 Size: 857 1697 3423 Fragments arranged by size: 3423 1697 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 4564 4938 5084 387 Size: 1064 138 521 2454 374 146 1280 Fragments arranged by size: 2454 1280 1064 521 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2147 2234 2879 4040 4637 5424 Size: 393 374 141 87 645 1161 597 787 Cuts at: 5424 5692 1239 Size: 268 1524 Fragments arranged by size: 1524 1161 787 645 597 393 374 268 141 87 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FspI TGC'GCA Cuts at: 2841 4483 2841 Size: 1642 4335 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2371 2403 4618 4650 4692 Size: 32 135 32 799 32 2215 32 42 Cuts at: 4692 4724 1373 Size: 32 2626 Fragments arranged by size: 2626 2215 799 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3129 3302 3129 Size: 173 5804 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3040 5091 5275 1305 Size: 1735 2051 184 2007 Fragments arranged by size: 2051 2007 1735 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2451 3935 4294 4453 518 Size: 648 1285 1484 359 159 2042 Fragments arranged by size: 2042 1484 1285 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2496 2766 2866 3393 4070 5218 5463 1606 Size: 890 270 100 527 677 1148 245 2120 Fragments arranged by size: 2120 1148 890 677 527 270 245 100 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5425 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2825 2846 3335 4880 1132 Size: 97 1596 21 489 1545 2229 Fragments arranged by size: 2229 1596 1545 489 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 3206 1108 Size: 1416 682 3879 Fragments arranged by size: 3879 1416 682 PfoI T'CCnGG_A Cuts at: 2022 2022 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PmlI CAC'GTG Cuts at: 2067 2067 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PstI C_TGCA'G Cuts at: 830 2751 3329 830 Size: 1921 578 3478 Fragments arranged by size: 3478 1921 578 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2766 2866 2766 Size: 100 5877 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2210 2210 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2747 2881 3161 3325 4460 5141 5332 Size: 219 1702 134 280 164 1135 681 191 Cuts at: 5332 826 Size: 1471 Fragments arranged by size: 1702 1471 1135 681 280 219 191 164 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2475 2953 4081 4982 5244 820 Size: 197 926 532 478 1128 901 262 1553 Fragments arranged by size: 1553 1128 926 901 532 478 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 2257 2649 1517 Size: 740 392 4845 Fragments arranged by size: 4845 740 392 StyI C'CwwG_G Cuts at: 513 1065 3416 513 Size: 552 2351 3074 Fragments arranged by size: 3074 2351 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2709 2876 4004 4021 4174 4359 4804 Size: 1255 1129 167 1128 17 153 185 445 Cuts at: 4804 325 Size: 1498 Fragments arranged by size: 1498 1255 1129 1128 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2551 2824 4223 Size: 274 80 33 51 1443 574 273 1399 Cuts at: 4223 5935 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1399 574 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 2947 3313 2947 Size: 366 5611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2289 2289 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 2329 3150 4106 2329 Size: 821 956 4200 Fragments arranged by size: 4200 956 821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI