(Circular) (Six-base) MAPSORT of: pFN21AB3963.seq Check: 7880 from: 1 to: 6844 pFN21AB3963 6844 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2953 2953 Size: 6844 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3366 4654 278 Size: 53 83 186 936 1830 1288 2468 Fragments arranged by size: 2468 1830 1288 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3642 4187 1937 Size: 1705 545 4594 Fragments arranged by size: 4594 1705 545 Acc65I G'GTAC_C Cuts at: 4165 4165 Size: 6844 AclI AA'CG_TT Cuts at: 1563 4971 5344 1563 Size: 3408 373 3063 Fragments arranged by size: 3408 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2152 3795 4078 4111 4907 6291 6559 1632 Size: 520 1643 283 33 796 1384 268 1917 Fragments arranged by size: 1917 1643 1384 796 520 283 268 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6647 AflIII A'CryG_T Cuts at: 1203 2895 5743 6720 1203 Size: 1692 2848 977 1327 Fragments arranged by size: 2848 1692 1327 977 AgeI A'CCGG_T Cuts at: 5675 5675 Size: 6844 AhdI GACnn_n'nnGTC Cuts at: 3628 5573 3628 Size: 1945 4899 AleI CACnn'nnGTG Cuts at: 1166 2792 2936 3614 1166 Size: 1626 144 678 4396 Fragments arranged by size: 4396 1626 678 144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3506 1184 Size: 2322 4522 AlwNI CAG_nnn'CTG Cuts at: 2053 2175 3773 4587 6159 2053 Size: 122 1598 814 1572 2738 Fragments arranged by size: 2738 1598 1572 814 122 ApaI G_GGCC'C Cuts at: 3428 4087 3428 Size: 659 6185 ApaLI G'TGCA_C Cuts at: 4901 6057 4901 Size: 1156 5688 ApoI r'AATT_y Cuts at: 1493 2132 2381 2562 3089 3303 3860 4054 4153 Size: 639 249 181 527 214 557 194 99 Cuts at: 4153 4379 4531 6640 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 639 557 527 249 226 214 194 181 152 99 AseI AT'TA_AT Cuts at: 160 1032 5398 160 Size: 872 4366 1606 Fragments arranged by size: 4366 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 AvaI C'yCGr_G Cuts at: 1870 1943 2209 2678 3224 4169 1870 Size: 73 266 469 546 945 4545 Fragments arranged by size: 4545 945 546 469 266 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4025 4058 4025 Size: 33 6811 BamHI G'GATC_C Cuts at: 4174 4541 4174 Size: 367 6477 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2689 2786 4165 5620 618 Size: 325 217 627 902 97 1379 1455 1842 Fragments arranged by size: 1842 1455 1379 902 627 325 217 97 BanII G_rGCy'C Cuts at: 1391 2216 2363 3428 4087 4163 1391 Size: 825 147 1065 659 76 4072 Fragments arranged by size: 4072 1065 825 659 147 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3351 3424 928 Size: 971 1452 73 4348 Fragments arranged by size: 4348 1452 971 73 BbvCI CC'TCA_GC Cuts at: 4118 4118 Size: 6844 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2473 2507 2659 2693 5035 Size: 34 50 34 452 34 152 34 2342 Cuts at: 5035 5069 1903 Size: 34 3678 Fragments arranged by size: 3678 2342 452 152 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4736 5946 4736 Size: 1210 5634 BclI T'GATC_A Cuts at: 1548 2713 2991 3076 3327 1548 Size: 1165 278 85 251 5065 Fragments arranged by size: 5065 1165 278 251 85 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5455 136 Size: 107 122 71 631 605 3783 1525 Fragments arranged by size: 3783 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3903 4024 6839 3903 Size: 121 2815 3908 Fragments arranged by size: 3908 2815 121 BlpI GC'TnA_GC Cuts at: 4132 4261 4132 Size: 129 6715 Bme1580I G_kGCm'C Cuts at: 1790 3428 3707 4087 4905 6061 1790 Size: 1638 279 380 818 1156 2573 Fragments arranged by size: 2573 1638 1156 818 380 279 BmgBI CAC'GTC Cuts at: 1318 2764 3736 1318 Size: 1446 972 4426 Fragments arranged by size: 4426 1446 972 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3422 3717 4575 Size: 416 53 83 399 24 2005 295 858 Cuts at: 4575 5533 442 Size: 958 1753 Fragments arranged by size: 2005 1753 958 858 416 399 295 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6844 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3490 3490 Size: 6844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3749 5504 1239 Size: 2510 1755 2579 Fragments arranged by size: 2579 2510 1755 Bpu10I CC'TnA_GC Cuts at: 4118 4118 Size: 6844 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2988 4969 5834 6132 2988 Size: 1981 865 298 3700 Fragments arranged by size: 3700 1981 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2212 2645 5507 882 Size: 617 713 433 2862 2219 Fragments arranged by size: 2862 2219 713 617 433 BsaAI yAC'GTr Cuts at: 493 1204 4635 493 Size: 711 3431 2702 Fragments arranged by size: 3431 2702 711 BsaBI GATnn'nnATC Cuts at: 3059 3831 6838 3059 Size: 772 3007 3065 Fragments arranged by size: 3065 3007 772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2873 3363 4651 5033 Size: 53 83 186 936 1340 490 1288 382 Cuts at: 5033 5668 275 Size: 635 1451 Fragments arranged by size: 1451 1340 1288 936 635 490 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5277 5675 5949 6096 6507 5277 Size: 398 274 147 411 5614 Fragments arranged by size: 5614 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2913 2943 3129 3159 3503 Size: 30 165 30 1510 30 186 30 344 Cuts at: 3503 3533 1178 Size: 30 4489 Fragments arranged by size: 4489 1510 344 186 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4106 1186 Size: 2920 3924 BseYI C'CCAG_C Cuts at: 2827 3377 3562 6047 6543 2827 Size: 550 185 2485 496 3128 Fragments arranged by size: 3128 2485 550 496 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2068 1163 Size: 573 332 5939 Fragments arranged by size: 5939 573 332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2732 4294 5055 6083 1540 Size: 275 186 731 1562 761 1028 2301 Fragments arranged by size: 2301 1562 1028 761 731 275 186 BsiHKAI G_wGCw'C Cuts at: 4046 4905 4990 6061 4046 Size: 859 85 1071 4829 Fragments arranged by size: 4829 1071 859 85 BsmI GAATG_Cn' Cuts at: 3508 4355 4448 3508 Size: 847 93 5904 Fragments arranged by size: 5904 847 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2599 3370 2599 Size: 771 6073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2363 3428 3707 4046 4087 4163 Size: 399 426 147 1065 279 339 41 76 Cuts at: 4163 4905 4990 6061 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1065 742 426 399 339 279 147 85 76 41 BspEI T'CCGG_A Cuts at: 6507 6507 Size: 6844 BspHI T'CATG_A Cuts at: 3057 4733 6463 3057 Size: 1676 1730 3438 Fragments arranged by size: 3438 1730 1676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2953 3557 3981 4199 844 Size: 2109 604 424 218 3489 Fragments arranged by size: 3489 2109 604 424 218 BsrBI CCG'CTC Cuts at: 1394 1823 2023 4731 1394 Size: 429 200 2708 3507 Fragments arranged by size: 3507 2708 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 5339 5513 65 Size: 1183 4091 174 1396 Fragments arranged by size: 4091 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2686 5488 5675 1700 Size: 110 876 2802 187 2869 Fragments arranged by size: 2869 2802 876 187 110 BsrGI T'GTAC_A Cuts at: 96 3654 96 Size: 3558 3286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6844 BssSI C'ACGA_G Cuts at: 2330 4904 5916 2330 Size: 2574 1012 3258 Fragments arranged by size: 3258 2574 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6844 BstBI TT'CG_AA Cuts at: 5648 5648 Size: 6844 BstEII G'GTnAC_C Cuts at: 1184 2775 3454 1184 Size: 1591 679 4574 Fragments arranged by size: 4574 1591 679 BstXI CCAn_nnnn'nTGG Cuts at: 3570 3570 Size: 6844 BstYI r'GATC_y Cuts at: 1972 3832 3850 3903 4024 4174 4541 4926 4943 Size: 1860 18 53 121 150 367 385 17 Cuts at: 4943 6395 6481 6493 6839 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1860 1452 385 367 346 150 121 86 53 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 3507 513 Size: 552 2442 3850 Fragments arranged by size: 3850 2442 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2250 487 Size: 683 56 1024 5081 Fragments arranged by size: 5081 1024 683 56 BtsI GCAGTG_nn' Cuts at: 2277 3657 4360 5154 5174 2277 Size: 1380 703 794 20 3947 Fragments arranged by size: 3947 1380 794 703 20 ClaI AT'CG_AT Cuts at: 1554 2907 3063 1554 Size: 1353 156 5335 Fragments arranged by size: 5335 1353 156 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6809 DraI TTT'AAA Cuts at: 2380 4148 2380 Size: 1768 5076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5851 809 Size: 507 4535 1802 Fragments arranged by size: 4535 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2758 3042 4291 Size: 54 1425 50 275 18 928 284 1249 Cuts at: 4291 5180 6809 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1249 928 889 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4291 1537 Size: 275 2479 4090 Fragments arranged by size: 4090 2479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3671 4130 4774 1353 Size: 2318 459 644 3423 Fragments arranged by size: 3423 2318 644 459 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5431 5805 5951 387 Size: 1064 138 3842 374 146 1280 Fragments arranged by size: 3842 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 3749 3795 4078 4111 4907 5504 Size: 393 520 1597 46 283 33 796 597 Cuts at: 5504 6291 6559 1239 Size: 787 268 1524 Fragments arranged by size: 1597 1524 796 787 597 520 393 283 268 46 33 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2916 2045 Size: 871 5973 EcoO109I rG'GnC_Cy Cuts at: 2747 3424 3469 3559 4083 4084 2747 Size: 677 45 90 524 1 5507 Fragments arranged by size: 5507 677 524 90 45 1 EcoRI G'AATT_C Cuts at: 2132 4054 4153 4531 2132 Size: 1922 99 378 4445 Fragments arranged by size: 4445 1922 378 99 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3290 3290 Size: 6844 FspI TGC'GCA Cuts at: 5350 5350 Size: 6844 HaeII r_GCGC'y Cuts at: 1678 5991 1678 Size: 4313 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2610 2642 2870 Size: 32 135 32 560 32 446 32 228 Cuts at: 2870 2902 2913 2945 3129 3161 3503 3535 3614 Size: 32 11 32 184 32 342 32 79 Cuts at: 3614 3646 5485 5517 5559 5591 1373 Size: 32 1839 32 42 32 2626 Fragments arranged by size: 2626 1839 560 446 342 228 184 135 79 42 32 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3418 3604 3643 4188 4434 669 Size: 988 281 1480 186 39 545 246 3079 Fragments arranged by size: 3079 1480 988 545 281 246 186 39 HindIII A'AGCT_T Cuts at: 748 748 Size: 6844 HpaI GTT'AAC Cuts at: 4434 4434 Size: 6844 KpnI G_GTAC'C Cuts at: 4169 4169 Size: 6844 MfeI C'AATT_G Cuts at: 3675 4443 3675 Size: 768 6076 MluI A'CGCG_T Cuts at: 6720 6720 Size: 6844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3007 5958 6142 1305 Size: 1702 2951 184 2007 Fragments arranged by size: 2951 2007 1702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5022 Fragments arranged by size: 5022 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 2795 2936 3056 3452 3614 3983 Size: 648 1626 3 141 120 396 162 369 Cuts at: 3983 4802 5161 5320 518 Size: 819 359 159 2042 Fragments arranged by size: 2042 1626 819 648 396 369 359 162 159 141 120 3 MspA1I CmG'CkG Cuts at: 1606 2129 3614 4260 4937 6085 6330 1606 Size: 523 1485 646 677 1148 245 2120 Fragments arranged by size: 2120 1485 1148 677 646 523 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6292 NdeI CA'TA_TG Cuts at: 387 387 Size: 6844 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6844 NotI GC'GGCC_GC Cuts at: 4291 4291 Size: 6844 NspI r_CATG'y Cuts at: 1132 1229 2899 4202 5747 1132 Size: 97 1670 1303 1545 2229 Fragments arranged by size: 2229 1670 1545 1303 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6844 PciI A'CATG_T Cuts at: 2895 5743 2895 Size: 2848 3996 PflMI CCAn_nnn'nTGG Cuts at: 1108 2767 1108 Size: 1659 5185 PfoI T'CCnGG_A Cuts at: 2089 3408 2089 Size: 1319 5525 PmeI GTTT'AAAC Cuts at: 4148 4148 Size: 6844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4913 4945 6426 6458 4913 Size: 32 1481 32 5299 Fragments arranged by size: 5299 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3469 3559 3469 Size: 90 6754 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6844 PsiI TTA'TAA Cuts at: 4414 4414 Size: 6844 PspOMI G'GGCC_C Cuts at: 3424 4083 3424 Size: 659 6185 PstI C_TGCA'G Cuts at: 830 2071 2272 3528 4052 4196 830 Size: 1241 201 1256 524 144 3478 Fragments arranged by size: 3478 1256 1241 524 201 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6844 PvuII CAG'CTG Cuts at: 2129 3614 2129 Size: 1485 5359 SalI G'TCGA_C Cuts at: 1936 3641 4186 1936 Size: 1705 545 4594 Fragments arranged by size: 4594 1705 545 SbfI CC_TGCA'GG Cuts at: 4052 4196 4052 Size: 144 6700 ScaI AGT'ACT Cuts at: 5092 6804 5092 Size: 1712 5132 SfcI C'TryA_G Cuts at: 826 1045 2067 2268 3524 4048 4192 5327 6008 Size: 219 1022 201 1256 524 144 1135 681 Cuts at: 6008 6199 826 Size: 191 1471 Fragments arranged by size: 1471 1256 1135 1022 681 524 219 201 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6844 SmaI CCC'GGG Cuts at: 1872 3226 4171 1872 Size: 1354 945 4545 Fragments arranged by size: 4545 1354 945 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 2678 3003 4948 5849 6111 Size: 197 926 266 469 325 1945 901 262 Cuts at: 6111 820 Size: 1553 Fragments arranged by size: 1945 1553 926 901 469 325 266 262 197 SnaBI TAC'GTA Cuts at: 493 4635 493 Size: 4142 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6844 SphI G_CATG'C Cuts at: 1132 4202 1132 Size: 3070 3774 SspI AAT'ATT Cuts at: 5 52 3580 4768 5 Size: 47 3528 1188 2081 Fragments arranged by size: 3528 2081 1188 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6844 StyI C'CwwG_G Cuts at: 513 1065 2610 4283 513 Size: 552 1545 1673 3074 Fragments arranged by size: 3074 1673 1545 552 SwaI ATTT'AAAT Cuts at: 2380 2380 Size: 6844 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2746 2794 2973 4021 4871 4888 5041 Size: 1255 1166 48 179 1048 850 17 153 Cuts at: 5041 5226 5671 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1166 1048 850 445 185 179 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3654 5090 6802 Size: 274 80 33 51 1443 1677 1436 1712 Cuts at: 6802 96 Size: 138 Fragments arranged by size: 1712 1677 1443 1436 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2526 2558 6804 6836 2526 Size: 32 4246 32 2534 Fragments arranged by size: 4246 2534 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2927 1316 Size: 1611 5233 XbaI T'CTAG_A Cuts at: 4180 4180 Size: 6844 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3616 3616 Size: 6844 XhoI C'TCGA_G Cuts at: 1943 2209 2678 1943 Size: 266 469 6109 Fragments arranged by size: 6109 469 266 XmaI C'CCGG_G Cuts at: 1870 3224 4169 1870 Size: 1354 945 4545 Fragments arranged by size: 4545 1354 945 XmnI GAAnn'nnTTC Cuts at: 2108 4973 2108 Size: 2865 3979 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3364 4652 276 Size: 53 83 186 936 1830 1288 2468 Fragments arranged by size: 2468 1830 1288 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI