Restriction map for
pFN21AB3962
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 426 GTCTAC GT^[AC][GT]AC
AflIII 15 ACATGT A^C[GA][CT]GT
AocI 1505 CCTCAGG CC^T[ACGT]AGG
Asp700I 279 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 96 TGGCCA TGG^CCA
BclI 198 TGATCA T^GATCA
BsaAI 173 CACGTA [CT]AC^GT[GA]
BseRI 370 GAGGAG GAGGAG(10/8)
BsgI 467 CTGCAC GTGCAG(16/14)
BsiEI 931 CGACCG CG[GA][CT]^CG
BspLU11I 15 ACATGT A^CATGT
BstAPI 460 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1047 AGATCC [GA]^GATC[CT]
Bsu36I 1505 CCTCAGG CC^T[ACGT]AGG
CfoI 85 GCGC GCG^C
DsaI 758 CCATGG C^C[GA][CT]GG
EaeI 96 TGGCCA [CT]^GGCC[GA]
Eco81I 1505 CCTCAGG CC^T[ACGT]AGG
FbaI 198 TGATCA T^GATCA
HapII 976 CCGG C^CGG
HhaI 85 GCGC GCG^C
HinP1I 85 GCGC G^CGC
HindIII 293 AAGCTT A^AGCTT
HpaII 976 CCGG C^CGG
MaeII 174 ACGT A^CGT
MfeI 620 CAATTG C^AATTG
MflI 1047 AGATCC [GA]^GATC[CT]
MluNI 96 TGGCCA TGG^CCA
MscI 96 TGGCCA TGG^CCA
MspI 976 CCGG C^CGG
MunI 620 CAATTG C^AATTG
NcoI 758 CCATGG C^CATGG
PshAI 932 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1031 GAAGAGC GCTCTTC(1/4)
ScaI 583 AGTACT AGT^ACT
XbaI 324 TCTAGA T^CTAGA
XcmI 1008 CCAAAGTACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1047 AGATCC [GA]^GATC[CT]
XmnI 279 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1048 GATCC GGATC(4/5)
117 GGATC
AlwNI 1406 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1454 CAGAGCCTG
AspI 181 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
912 GACTCAGTC
BanI 600 GGCACC G^G[CT][GA]CC
762 GGTGCC
BbsI 1153 GAAGAC GAAGAC(2/6)
1315 GAAGAC
BfaI 325 CTAG C^TAG
767 CTAG
BpmI 860 CTCCAG CTGGAG(16/14)
983 CTCCAG
BpuAI 1153 GAAGAC GAAGAC(2/6)
1315 GAAGAC
BsmFI 625 GTCCC GGGAC(10/14)
736 GTCCC
DraII 41 AGGACCT [GA]G^G[ACGT]CC[CT]
786 AGGTCCT
EarI 647 CTCTTC CTCTTC(1/4)
1031 GAAGAG
EcoNI 608 CCTCTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
628 CCTGTACCAGG
EcoO109I 41 AGGACCT [GA]G^G[ACGT]CC[CT]
786 AGGTCCT
EcoRI 21 GAATTC G^AATTC
283 GAATTC
EcoT14I 758 CCATGG C^C[AT][AT]GG
1400 CCAAGG
Ksp632I 647 CTCTTC CTCTTC(1/4)
1031 GAAGAG
MaeI 325 CTAG C^TAG
767 CTAG
NspI 15 ACATGT [GA]CATG^[CT]
720 GCATGT
PflFI 181 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
912 GACTCAGTC
PpuMI 41 AGGACCT [GA]G^G[AT]CC[CT]
786 AGGTCCT
PstI 731 CTGCAG CTGCA^G
1180 CTGCAG
StyI 758 CCATGG C^C[AT][AT]GG
1400 CCAAGG
TaqI 747 TCGA T^CGA
930 TCGA
Tth111I 181 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
912 GACTCAGTC
TthHB8I 747 TCGA T^CGA
930 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI