(Circular) (Six-base) MAPSORT of: pFN21AB3956.seq Check: 4414 from: 1 to: 5857 pFN21AB3956 5857 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2071 3173 3920 5304 5572 1632 Size: 439 1102 747 1384 268 1917 Fragments arranged by size: 1917 1384 1102 747 439 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2819 1184 Size: 1635 4222 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 2741 Size: 32 5825 AlwNI CAG_nnn'CTG Cuts at: 2771 3600 5172 2771 Size: 829 1572 3456 Fragments arranged by size: 3456 1572 829 ApaI G_GGCC'C Cuts at: 2708 2708 Size: 5857 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2745 3182 1870 Size: 73 802 437 4545 Fragments arranged by size: 4545 802 437 73 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2869 3118 3178 4633 618 Size: 325 217 627 1082 249 60 1455 1842 Fragments arranged by size: 1842 1455 1082 627 325 249 217 60 BanII G_rGCy'C Cuts at: 1391 2708 2754 3176 1391 Size: 1317 46 422 4072 Fragments arranged by size: 4072 1317 422 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2825 928 Size: 971 926 3960 Fragments arranged by size: 3960 971 926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 2985 1548 Size: 1437 4420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2444 4468 Size: 107 122 71 631 605 616 156 2024 Cuts at: 4468 136 Size: 1525 Fragments arranged by size: 2024 1525 631 616 605 156 122 107 71 BglII A'GATC_T Cuts at: 2619 2634 5852 2619 Size: 15 3218 2624 Fragments arranged by size: 3218 2624 15 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 2708 2872 3123 3918 5074 1790 Size: 918 164 251 795 1156 2573 Fragments arranged by size: 2573 1156 918 795 251 164 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3588 4546 442 Size: 416 53 83 399 24 2171 958 1753 Fragments arranged by size: 2171 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4517 1239 Size: 3278 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2347 2380 2953 3648 493 Size: 711 1143 33 573 695 2702 Fragments arranged by size: 2702 1143 711 695 573 33 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3061 3664 4046 4681 Size: 53 83 186 936 1528 603 382 635 Cuts at: 4681 275 Size: 1451 Fragments arranged by size: 1528 1451 936 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 2741 2771 1178 Size: 30 165 30 648 30 660 30 4264 Fragments arranged by size: 4264 660 648 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 1186 Size: 873 4984 BseYI C'CCAG_C Cuts at: 2865 3069 5060 5556 2865 Size: 204 1991 496 3166 Fragments arranged by size: 3166 1991 496 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3038 1163 Size: 573 1302 3982 Fragments arranged by size: 3982 1302 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 2754 2835 3918 4003 5074 2385 Size: 369 81 1083 85 1071 3168 Fragments arranged by size: 3168 1083 1071 369 85 81 BsmI GAATG_Cn' Cuts at: 2841 3368 3461 2841 Size: 527 93 5237 Fragments arranged by size: 5237 527 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2419 2431 2419 Size: 12 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2708 2754 2835 2872 3123 3176 Size: 399 595 323 46 81 37 251 53 Cuts at: 3176 3918 4003 5074 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 399 323 251 85 81 53 46 37 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2412 3212 844 Size: 1568 800 3489 Fragments arranged by size: 3489 1568 800 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 2277 2448 2553 4352 4526 65 Size: 1183 927 102 171 105 1799 174 1396 Fragments arranged by size: 1799 1396 1183 927 174 171 105 102 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 2713 1184 Size: 1529 4328 BstYI r'GATC_y Cuts at: 1972 2619 2634 2739 3187 3554 3939 3956 5408 Size: 647 15 105 448 367 385 17 1452 Cuts at: 5408 5494 5506 5852 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 647 448 385 367 346 105 86 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2492 513 Size: 552 939 488 3878 Fragments arranged by size: 3878 939 552 488 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2950 487 Size: 683 56 1724 3394 Fragments arranged by size: 3394 1724 683 56 BtsI GCAGTG_nn' Cuts at: 2940 3373 4167 4187 2940 Size: 433 794 20 4610 Fragments arranged by size: 4610 794 433 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DraIII CAC_nnn'GTG Cuts at: 2997 2997 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2310 2374 Size: 54 1425 50 275 18 459 21 64 Cuts at: 2374 2445 2629 3304 4193 5822 8 Size: 71 184 675 889 1629 43 Fragments arranged by size: 1629 1425 889 675 459 275 184 71 64 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2684 3126 3787 1353 Size: 1331 442 661 3423 Fragments arranged by size: 3423 1331 661 442 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2752 2752 Size: 5857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 3173 3920 4517 5304 5572 1239 Size: 393 439 1102 747 597 787 268 1524 Fragments arranged by size: 1524 1102 787 747 597 439 393 268 EcoO109I rG'GnC_Cy Cuts at: 2705 2705 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 EcoRV GAT'ATC Cuts at: 2465 2465 Size: 5857 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2689 2689 Size: 5857 FspI TGC'GCA Cuts at: 2258 2408 4363 2258 Size: 150 1955 3752 Fragments arranged by size: 3752 1955 150 HaeII r_GCGC'y Cuts at: 1678 5004 1678 Size: 3326 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2488 2520 4498 Size: 32 135 32 479 32 405 32 1978 Cuts at: 4498 4530 4572 4604 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 479 405 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2398 3201 3447 669 Size: 988 281 460 803 246 3079 Fragments arranged by size: 3079 988 803 460 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 4971 5155 1305 Size: 1017 2649 184 2007 Fragments arranged by size: 2649 2007 1017 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 2312 2376 2447 2631 Size: 54 1425 343 459 21 64 71 184 Cuts at: 2631 10 Size: 3236 Fragments arranged by size: 3236 1425 459 343 184 71 64 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3815 4174 4333 518 Size: 648 1803 846 359 159 2042 Fragments arranged by size: 2042 1803 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3073 3273 3950 5098 5343 1606 Size: 1467 200 677 1148 245 2120 Fragments arranged by size: 2120 1467 1148 677 245 200 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3215 4760 1132 Size: 97 1986 1545 2229 Fragments arranged by size: 2229 1986 1545 97 PasI CC'CwG_GG Cuts at: 1348 2771 1348 Size: 1423 4434 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PmlI CAC'GTG Cuts at: 2380 2380 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2741 2773 3926 3958 5439 5471 2741 Size: 32 1153 32 1481 32 3127 Fragments arranged by size: 3127 1481 1153 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2704 2704 Size: 5857 PstI C_TGCA'G Cuts at: 830 3209 830 Size: 2379 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 SacI G_AGCT'C Cuts at: 2754 2754 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 3032 4105 5817 3032 Size: 1073 1712 3072 Fragments arranged by size: 3072 1712 1073 SfcI C'TryA_G Cuts at: 826 1045 3205 4340 5021 5212 826 Size: 219 2160 1135 681 191 1471 Fragments arranged by size: 2160 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 2747 3184 1872 Size: 875 437 4545 Fragments arranged by size: 4545 875 437 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2004 2529 3296 513 Size: 552 939 525 767 3074 Fragments arranged by size: 3074 939 767 552 525 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2053 3884 3901 4054 4239 4684 325 Size: 1255 473 1831 17 153 185 445 1498 Fragments arranged by size: 1831 1498 1255 473 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 4103 5815 Size: 274 80 33 51 1443 1053 1073 1712 Cuts at: 5815 96 Size: 138 Fragments arranged by size: 1712 1443 1073 1053 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2440 3133 2440 Size: 693 5164 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2745 3182 1870 Size: 875 437 4545 Fragments arranged by size: 4545 875 437 XmnI GAAnn'nnTTC Cuts at: 2621 3032 3986 2621 Size: 411 954 4492 Fragments arranged by size: 4492 954 411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI