Restriction map for
pFN21AB3715
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# NO CUT ENZYMES ( 167 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeIII MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 430 CGCG CG^CG
AcsI 37 AAATTC [GA]^AATT[CT]
AflIII 504 ACGTGT A^C[GA][CT]GT
AhdI 145 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 37 AAATTC [GA]^AATT[CT]
AspEI 145 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 325 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 592 GAGCCC G[GA]GC[CT]^C
BcgI 492 GCAGCTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 592 GAGCCC G[AGT]GC[ACT]^C
BsaI 460 GAGACC GGTCTC(1/5)
BseRI 415 GAGGAG GAGGAG(10/8)
BsiYI 311 CCTCTAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 311 CCTCTAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 80 CGTCTC CGTCTC(1/5)
Bsp1286I 592 GAGCCC G[AGT]GC[ACT]^C
BssHII 429 GCGCGC G^CGCGC
BstUI 430 CGCG CG^CG
BstXI 161 CCATGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 257 CCACGG C^C[GA][CT]GG
EaeI 599 CGGCCA [CT]^GGCC[GA]
Eam1105I 145 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeIII 600 GGCC GG^CC
MslI 363 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 206 CAGCTG C[AC]G^C[GT]G
MvnI 430 CGCG CG^CG
PflFI 325 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 253 GACTC GAGTC(4/5)
PstI 190 CTGCAG CTGCA^G
PvuII 206 CAGCTG CAG^CTG
SfcI 190 CTGCAG C^T[GA][CT]AG
TfiI 24 GAATC G^A[AT]TC
Tsp509I 38 AATT ^AATT
Tth111I 325 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 78 GACGTC GACGT^C
328 GACGTC
AlwI 234 GATCC GGATC(4/5)
320 GGATC
AvaII 174 GGACC G^G[AT]CC
384 GGACC
BbsI 75 GAAGAC GAAGAC(2/6)
471 GAAGAC
BpuAI 75 GAAGAC GAAGAC(2/6)
471 GAAGAC
BsaBI 405 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
564 GATCGACATC
BsmAI 460 GAGAC GTCTC(1/5)
81 GTCTC
BsmFI 200 GGGAC GGGAC(10/14)
251 GGGAC
BsrI 475 ACTGG ACTGG(1/-1)
464 CCAGT
BstF5I 246 CATCC GGATG(2/0)
560 GGATG
Cac8I 429 GCGCGC GC[ACGT]^[ACGT]GC
601 GCCAGC
CfoI 429 GCGC GCG^C
431 GCGC
Cfr13I 174 GGACC G^G[ACGT]CC
384 GGACC
FokI 246 CATCC GGATG(9/13)
560 GGATG
HhaI 429 GCGC GCG^C
431 GCGC
HinP1I 429 GCGC G^CGC
431 GCGC
HinfI 24 GAATC G^A[ACGT]TC
253 GACTC
MamI 405 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
564 GATCGACATC
MboII 75 GAAGA GAAGA(8/7)
471 GAAGA
NlaIV 221 GGTTCC GG[ACGT]^[ACGT]CC
591 GGAGCC
Sau96I 174 GGACC G^G[ACGT]CC
384 GGACC
XcmI 126 CCAGGCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
257 CCACGGTGGCTGTGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AcyI AfaI AluI BbvI
BcnI BsaHI BsaJI BssKI BstNI
DdeI DpnII EcoRII Fnu4HI HapII
HgaI Hin1I HpaII ItaI MaeII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIII RsaI Sau3AI
ScrFI TaqI TseI TspRI TthHB8I