(Circular) (Six-base) MAPSORT of: pFN21AB3666.seq Check: 7060 from: 1 to: 6919 pFN21AB3666 6919 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3676 3676 Size: 6919 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2046 4729 278 Size: 53 83 186 936 510 2683 2468 Fragments arranged by size: 2683 2468 936 510 186 83 53 AccI GT'mk_AC Cuts at: 1937 4262 1937 Size: 2325 4594 Acc65I G'GTAC_C Cuts at: 4240 4240 Size: 6919 AclI AA'CG_TT Cuts at: 1563 2457 5046 5419 1563 Size: 894 2589 373 3063 Fragments arranged by size: 3063 2589 894 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3603 3825 4040 4982 6366 6634 1632 Size: 1971 222 215 942 1384 268 1917 Fragments arranged by size: 1971 1917 1384 942 268 222 215 AflII C'TTAA_G Cuts at: 820 1017 2438 820 Size: 197 1421 5301 Fragments arranged by size: 5301 1421 197 AflIII A'CryG_T Cuts at: 1203 5818 6795 1203 Size: 4615 977 1327 Fragments arranged by size: 4615 1327 977 AgeI A'CCGG_T Cuts at: 5750 5750 Size: 6919 AhdI GACnn_n'nnGTC Cuts at: 5648 5648 Size: 6919 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6919 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3735 3767 3735 Size: 32 6887 AlwNI CAG_nnn'CTG Cuts at: 3902 4118 4662 6234 3902 Size: 216 544 1572 4587 Fragments arranged by size: 4587 1572 544 216 ApaLI G'TGCA_C Cuts at: 4976 6132 4976 Size: 1156 5763 ApoI r'AATT_y Cuts at: 1493 2318 2361 2394 2637 3059 3138 4228 4454 Size: 825 43 33 243 422 79 1090 226 Cuts at: 4454 4606 6715 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1090 825 422 243 226 152 79 43 33 AseI AT'TA_AT Cuts at: 160 1032 5473 160 Size: 872 4441 1606 Fragments arranged by size: 4441 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 AvaI C'yCGr_G Cuts at: 1870 1943 3924 4244 1870 Size: 73 1981 320 4545 Fragments arranged by size: 4545 1981 320 73 BamHI G'GATC_C Cuts at: 4249 4616 4249 Size: 367 6552 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2500 3664 4240 5695 Size: 325 217 627 273 440 1164 576 1455 Cuts at: 5695 618 Size: 1842 Fragments arranged by size: 1842 1455 1164 627 576 440 325 273 217 BanII G_rGCy'C Cuts at: 1391 2123 2550 3324 3684 4238 1391 Size: 732 427 774 360 554 4072 Fragments arranged by size: 4072 774 732 554 427 360 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 6919 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2038 3446 3633 928 Size: 971 139 1408 187 4214 Fragments arranged by size: 4214 1408 971 187 139 BbvCI CC'TCA_GC Cuts at: 3702 3702 Size: 6919 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5110 5144 1903 Size: 34 3173 34 3678 Fragments arranged by size: 3678 3173 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4811 6021 4811 Size: 1210 5709 BclI T'GATC_A Cuts at: 1548 2512 1548 Size: 964 5955 BfrBI ATG'CAT Cuts at: 2926 4024 2926 Size: 1098 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5530 136 Size: 107 122 71 631 605 337 3521 1525 Fragments arranged by size: 3521 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6914 6914 Size: 6919 BlpI GC'TnA_GC Cuts at: 4336 4336 Size: 6919 Bme1580I G_kGCm'C Cuts at: 1790 4980 6136 1790 Size: 3190 1156 2573 Fragments arranged by size: 3190 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6919 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4650 5608 442 Size: 416 53 83 399 24 3233 958 1753 Fragments arranged by size: 3233 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3605 1056 Size: 2549 4370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2021 3747 3796 5579 1239 Size: 782 1726 49 1783 2579 Fragments arranged by size: 2579 1783 1726 782 49 Bpu10I CC'TnA_GC Cuts at: 3702 3702 Size: 6919 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2260 5044 5909 6207 2260 Size: 2784 865 298 2972 Fragments arranged by size: 2972 2784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2179 5582 882 Size: 617 680 3403 2219 Fragments arranged by size: 3403 2219 680 617 BsaAI yAC'GTr Cuts at: 493 1204 4710 493 Size: 711 3506 2702 Fragments arranged by size: 3506 2702 711 BsaBI GATnn'nnATC Cuts at: 6913 6913 Size: 6919 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2061 4726 5108 Size: 53 83 186 936 510 18 2665 382 Cuts at: 5108 5743 275 Size: 635 1451 Fragments arranged by size: 2665 1451 936 635 510 382 186 83 53 18 BsaWI w'CCGG_w Cuts at: 5352 5750 6024 6171 6582 5352 Size: 398 274 147 411 5689 Fragments arranged by size: 5689 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3735 3765 1178 Size: 30 165 30 2332 30 4332 Fragments arranged by size: 4332 2332 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6919 BseYI C'CCAG_C Cuts at: 3323 6122 6618 3323 Size: 2799 496 3624 Fragments arranged by size: 3624 2799 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4369 5130 6158 1540 Size: 275 186 2368 761 1028 2301 Fragments arranged by size: 2368 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2123 2550 4980 5065 6136 2123 Size: 427 2430 85 1071 2906 Fragments arranged by size: 2906 2430 1071 427 85 BsmI GAATG_Cn' Cuts at: 3789 4430 4523 3789 Size: 641 93 6185 Fragments arranged by size: 6185 641 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2550 3324 3684 4238 4980 5065 Size: 399 333 427 774 360 554 742 85 Cuts at: 5065 6136 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 774 742 554 427 399 360 333 85 BspEI T'CCGG_A Cuts at: 6582 6582 Size: 6919 BspHI T'CATG_A Cuts at: 3349 3424 4808 6538 3349 Size: 75 1384 1730 3730 Fragments arranged by size: 3730 1730 1384 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 3676 3891 4274 844 Size: 1316 1516 215 383 3489 Fragments arranged by size: 3489 1516 1316 383 215 BsrBI CCG'CTC Cuts at: 1394 1823 4806 1394 Size: 429 2983 3507 Fragments arranged by size: 3507 2983 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5414 5588 65 Size: 1183 4166 174 1396 Fragments arranged by size: 4166 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5563 5750 1700 Size: 110 3753 187 2869 Fragments arranged by size: 3753 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6919 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6919 BssSI C'ACGA_G Cuts at: 3630 4979 5991 3630 Size: 1349 1012 4558 Fragments arranged by size: 4558 1349 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6919 BstBI TT'CG_AA Cuts at: 5723 5723 Size: 6919 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6919 BstYI r'GATC_y Cuts at: 1972 2653 4249 4616 5001 5018 6470 6556 6568 Size: 681 1596 367 385 17 1452 86 12 Cuts at: 6568 6914 1972 Size: 346 1977 Fragments arranged by size: 1977 1596 1452 681 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2520 3270 2520 Size: 750 6169 BtgI C'CryG_G Cuts at: 513 1065 2004 2721 3018 513 Size: 552 939 717 297 4414 Fragments arranged by size: 4414 939 717 552 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6180 Fragments arranged by size: 6180 683 56 BtsI GCAGTG_nn' Cuts at: 2595 4435 5229 5249 2595 Size: 1840 794 20 4265 Fragments arranged by size: 4265 1840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6919 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6884 DraI TTT'AAA Cuts at: 4223 4223 Size: 6919 DraIII CAC_nnn'GTG Cuts at: 3197 3197 Size: 6919 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5926 809 Size: 507 4610 1802 Fragments arranged by size: 4610 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2871 4366 5255 Size: 54 1425 50 275 18 1041 1495 889 Cuts at: 5255 6884 8 Size: 1629 43 Fragments arranged by size: 1629 1495 1425 1041 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4366 1537 Size: 275 2554 4090 Fragments arranged by size: 4090 2554 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 3000 3312 3801 4059 4849 1353 Size: 1451 196 312 489 258 790 3423 Fragments arranged by size: 3423 1451 790 489 312 258 196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5506 5880 6026 387 Size: 1064 138 3917 374 146 1280 Fragments arranged by size: 3917 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2121 2548 2121 Size: 427 6492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 3603 3747 3796 3825 4040 4982 Size: 393 389 1582 144 49 29 215 942 Cuts at: 4982 5579 6366 6634 1239 Size: 597 787 268 1524 Fragments arranged by size: 1582 1524 942 787 597 393 389 268 215 144 49 29 EcoO109I rG'GnC_Cy Cuts at: 2149 2149 Size: 6919 EcoRI G'AATT_C Cuts at: 4228 4606 4228 Size: 378 6541 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3054 4070 3054 Size: 1016 5903 FspI TGC'GCA Cuts at: 5425 5425 Size: 6919 HaeII r_GCGC'y Cuts at: 1678 2015 2064 6066 1678 Size: 337 49 4002 2531 Fragments arranged by size: 4002 2531 337 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5560 5592 5634 5666 1373 Size: 32 135 32 3988 32 42 32 2626 Fragments arranged by size: 3988 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2779 4263 4509 669 Size: 988 281 841 1484 246 3079 Fragments arranged by size: 3079 1484 988 841 281 246 HindIII A'AGCT_T Cuts at: 748 2339 748 Size: 1591 5328 HpaI GTT'AAC Cuts at: 2779 4509 2779 Size: 1730 5189 KasI G'GCGC_C Cuts at: 2060 2060 Size: 6919 KpnI G_GTAC'C Cuts at: 4244 4244 Size: 6919 MfeI C'AATT_G Cuts at: 3507 4518 3507 Size: 1011 5908 MluI A'CGCG_T Cuts at: 6795 6795 Size: 6919 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3070 3721 6033 6217 1305 Size: 1765 651 2312 184 2007 Fragments arranged by size: 2312 2007 1765 651 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2873 10 Size: 54 1425 343 1041 4056 Fragments arranged by size: 4056 1425 1041 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4877 5236 5395 518 Size: 648 3711 359 159 2042 Fragments arranged by size: 3711 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2112 3333 3623 3692 4078 4151 4335 Size: 471 35 1221 290 69 386 73 184 Cuts at: 4335 5012 6160 6405 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1221 1148 677 471 386 290 245 184 73 69 35 NarI GG'CG_CC Cuts at: 2061 2061 Size: 6919 NcoI C'CATG_G Cuts at: 513 1065 2004 2721 513 Size: 552 939 717 4711 Fragments arranged by size: 4711 939 717 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6919 NheI G'CTAG_C Cuts at: 1052 3601 1052 Size: 2549 4370 NotI GC'GGCC_GC Cuts at: 4366 4366 Size: 6919 NsiI A_TGCA'T Cuts at: 2928 4026 2928 Size: 1098 5821 NspI r_CATG'y Cuts at: 1132 1229 4277 5822 1132 Size: 97 3048 1545 2229 Fragments arranged by size: 3048 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6919 PciI A'CATG_T Cuts at: 5818 5818 Size: 6919 PflMI CCAn_nnn'nTGG Cuts at: 1108 3197 1108 Size: 2089 4830 PfoI T'CCnGG_A Cuts at: 2037 4200 2037 Size: 2163 4756 PmeI GTTT'AAAC Cuts at: 4223 4223 Size: 6919 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3735 3767 4988 5020 6501 6533 3735 Size: 32 1221 32 1481 32 4121 Fragments arranged by size: 4121 1481 1221 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2149 Size: 6919 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6919 PsiI TTA'TAA Cuts at: 4489 4489 Size: 6919 PstI C_TGCA'G Cuts at: 830 4271 830 Size: 3441 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6919 PvuII CAG'CTG Cuts at: 2077 3623 4078 4151 2077 Size: 1546 455 73 4845 Fragments arranged by size: 4845 1546 455 73 SacI G_AGCT'C Cuts at: 2123 2550 2123 Size: 427 6492 SalI G'TCGA_C Cuts at: 1936 4261 1936 Size: 2325 4594 SapI GCTCTTCn'nnn_ Cuts at: 3312 3312 Size: 6919 SbfI CC_TGCA'GG Cuts at: 4271 4271 Size: 6919 ScaI AGT'ACT Cuts at: 5167 6879 5167 Size: 1712 5207 SfcI C'TryA_G Cuts at: 826 1045 2505 3379 4267 5402 6083 6274 826 Size: 219 1460 874 888 1135 681 191 1471 Fragments arranged by size: 1471 1460 1135 888 874 681 219 191 SfoI GGC'GCC Cuts at: 2062 2062 Size: 6919 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6919 SmaI CCC'GGG Cuts at: 1872 4246 1872 Size: 2374 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2275 2438 5023 5924 6186 820 Size: 197 926 332 163 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 332 262 197 163 SnaBI TAC'GTA Cuts at: 493 4710 493 Size: 4217 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6919 SphI G_CATG'C Cuts at: 1132 4277 1132 Size: 3145 3774 SspI AAT'ATT Cuts at: 5 52 3074 3994 4843 5 Size: 47 3022 920 849 2081 Fragments arranged by size: 3022 2081 920 849 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6919 StyI C'CwwG_G Cuts at: 513 1065 2004 2496 2721 4358 513 Size: 552 939 492 225 1637 3074 Fragments arranged by size: 3074 1637 939 552 492 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2467 4946 4963 5116 5301 5746 325 Size: 1255 887 2479 17 153 185 445 1498 Fragments arranged by size: 2479 1498 1255 887 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3392 5165 6877 Size: 274 80 33 51 1443 1415 1773 1712 Cuts at: 6877 96 Size: 138 Fragments arranged by size: 1773 1712 1443 1415 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3520 3552 6879 6911 3520 Size: 32 3327 32 3528 Fragments arranged by size: 3528 3327 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6919 XbaI T'CTAG_A Cuts at: 3313 4255 3313 Size: 942 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3161 3439 3161 Size: 278 6641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6919 XmaI C'CCGG_G Cuts at: 1870 4244 1870 Size: 2374 4545 XmnI GAAnn'nnTTC Cuts at: 2430 3345 5048 2430 Size: 915 1703 4301 Fragments arranged by size: 4301 1703 915 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2044 4727 276 Size: 53 83 186 936 510 2683 2468 Fragments arranged by size: 2683 2468 936 510 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI