Restriction map for
pFN21AB3666
(Linear) (Six-base) MAPSORT of: KIBB3666.seq Check: 6310 from: 1 to: 2214
KIBB3666.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1671 2214
Size: 1671 543
AatII G_ACGT'C
Cuts at: 0 41 2214
Size: 41 2173
AclI AA'CG_TT
Cuts at: 0 452 2214
Size: 452 1762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1598 1820 2035 2214
Size: 1598 222 215 179
Fragments arranged by size:
1598 222 215 179
AflII C'TTAA_G
Cuts at: 0 433 2214
Size: 433 1781
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1730 1762 2214
Size: 1730 32 452
Fragments arranged by size:
1730 452 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1897 2113 2214
Size: 1897 216 101
Fragments arranged by size:
1897 216 101
ApoI r'AATT_y
Cuts at: 0 313 356 389 632 1054 1133 2214
Size: 313 43 33 243 422 79 1081
Fragments arranged by size:
1081 422 313 243 79 43 33
AvaI C'yCGr_G
Cuts at: 0 1919 2214
Size: 1919 295
BanI G'GyrC_C
Cuts at: 0 55 495 1659 2214
Size: 55 440 1164 555
Fragments arranged by size:
1164 555 440 55
BanII G_rGCy'C
Cuts at: 0 118 545 1319 1679 2214
Size: 118 427 774 360 535
Fragments arranged by size:
774 535 427 360 118
BbeI G_GCGC'C
Cuts at: 0 59 2214
Size: 59 2155
BbsI GAAGACnn'nnnn_
Cuts at: 0 33 1441 1628 2214
Size: 33 1408 187 586
Fragments arranged by size:
1408 586 187 33
BbvCI CC'TCA_GC
Cuts at: 0 1697 2214
Size: 1697 517
BclI T'GATC_A
Cuts at: 0 507 2214
Size: 507 1707
BfrBI ATG'CAT
Cuts at: 0 921 2019 2214
Size: 921 1098 195
Fragments arranged by size:
1098 921 195
BmtI G_CTAG'C
Cuts at: 0 1600 2214
Size: 1600 614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1742 1791 2214
Size: 16 1726 49 423
Fragments arranged by size:
1726 423 49 16
Bpu10I CC'TnA_GC
Cuts at: 0 1697 2214
Size: 1697 517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 2214
Size: 255 1959
BsaI GGTCTCn'nnnn_
Cuts at: 0 174 2214
Size: 174 2040
BsaHI Gr'CG_yC
Cuts at: 0 38 56 2214
Size: 38 18 2158
Fragments arranged by size:
2158 38 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1730 1760 2214
Size: 1730 30 454
Fragments arranged by size:
1730 454 30
BseYI C'CCAG_C
Cuts at: 0 1318 2214
Size: 1318 896
BsiHKAI G_wGCw'C
Cuts at: 0 118 545 2214
Size: 118 427 1669
Fragments arranged by size:
1669 427 118
BsmI GAATG_Cn'
Cuts at: 0 1784 2214
Size: 1784 430
Bsp1286I G_dGCh'C
Cuts at: 0 118 545 1319 1679 2214
Size: 118 427 774 360 535
Fragments arranged by size:
774 535 427 360 118
BspHI T'CATG_A
Cuts at: 0 1344 1419 2214
Size: 1344 75 795
Fragments arranged by size:
1344 795 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 1671 1886 2214
Size: 155 1516 215 328
Fragments arranged by size:
1516 328 215 155
BssSI C'ACGA_G
Cuts at: 0 1625 2214
Size: 1625 589
BstYI r'GATC_y
Cuts at: 0 648 2214
Size: 648 1566
Bsu36I CC'TnA_GG
Cuts at: 0 515 1265 2214
Size: 515 750 949
Fragments arranged by size:
949 750 515
BtgI C'CryG_G
Cuts at: 0 716 1013 2214
Size: 716 297 1201
Fragments arranged by size:
1201 716 297
BtsI GCAGTG_nn'
Cuts at: 0 590 2214
Size: 590 1624
DraIII CAC_nnn'GTG
Cuts at: 0 1192 2214
Size: 1192 1022
EaeI y'GGCC_r
Cuts at: 0 866 2214
Size: 866 1348
EarI CTCTTCn'nnn_
Cuts at: 0 799 995 1307 1796 2054 2214
Size: 799 196 312 489 258 160
Fragments arranged by size:
799 489 312 258 196 160
EcoICRI GAG'CTC
Cuts at: 0 116 543 2214
Size: 116 427 1671
Fragments arranged by size:
1671 427 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1598 1742 1791 1820 2035 2214
Size: 16 1582 144 49 29 215 179
Fragments arranged by size:
1582 215 179 144 49 29 16
EcoO109I rG'GnC_Cy
Cuts at: 0 144 2214
Size: 144 2070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1049 2065 2214
Size: 1049 1016 149
Fragments arranged by size:
1049 1016 149
HaeII r_GCGC'y
Cuts at: 0 10 59 2214
Size: 10 49 2155
Fragments arranged by size:
2155 49 10
HincII GTy'rAC
Cuts at: 0 774 2214
Size: 774 1440
HindIII A'AGCT_T
Cuts at: 0 334 2214
Size: 334 1880
HpaI GTT'AAC
Cuts at: 0 774 2214
Size: 774 1440
KasI G'GCGC_C
Cuts at: 0 55 2214
Size: 55 2159
MfeI C'AATT_G
Cuts at: 0 1502 2214
Size: 1502 712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 1716 2214
Size: 1065 651 498
Fragments arranged by size:
1065 651 498
MscI TGG'CCA
Cuts at: 0 868 2214
Size: 868 1346
MspA1I CmG'CkG
Cuts at: 0 72 107 1328 1618 1687 2073 2146 2214
Size: 72 35 1221 290 69 386 73 68
Fragments arranged by size:
1221 386 290 73 72 69 68 35
NarI GG'CG_CC
Cuts at: 0 56 2214
Size: 56 2158
NcoI C'CATG_G
Cuts at: 0 716 2214
Size: 716 1498
NheI G'CTAG_C
Cuts at: 0 1596 2214
Size: 1596 618
NsiI A_TGCA'T
Cuts at: 0 923 2021 2214
Size: 923 1098 193
Fragments arranged by size:
1098 923 193
PflMI CCAn_nnn'nTGG
Cuts at: 0 1192 2214
Size: 1192 1022
PfoI T'CCnGG_A
Cuts at: 0 32 2195 2214
Size: 32 2163 19
Fragments arranged by size:
2163 32 19
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1730 1762 2214
Size: 1730 32 452
Fragments arranged by size:
1730 452 32
PpuMI rG'GwC_Cy
Cuts at: 0 144 2214
Size: 144 2070
PvuII CAG'CTG
Cuts at: 0 72 1618 2073 2146 2214
Size: 72 1546 455 73 68
Fragments arranged by size:
1546 455 73 72 68
SacI G_AGCT'C
Cuts at: 0 118 545 2214
Size: 118 427 1669
Fragments arranged by size:
1669 427 118
SapI GCTCTTCn'nnn_
Cuts at: 0 1307 2214
Size: 1307 907
SfcI C'TryA_G
Cuts at: 0 500 1374 2214
Size: 500 874 840
Fragments arranged by size:
874 840 500
SfoI GGC'GCC
Cuts at: 0 57 2214
Size: 57 2157
SmlI C'TyrA_G
Cuts at: 0 270 433 2214
Size: 270 163 1781
Fragments arranged by size:
1781 270 163
SspI AAT'ATT
Cuts at: 0 1069 1989 2214
Size: 1069 920 225
Fragments arranged by size:
1069 920 225
StyI C'CwwG_G
Cuts at: 0 491 716 2214
Size: 491 225 1498
Fragments arranged by size:
1498 491 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 462 2214
Size: 462 1752
TatI w'GTAC_w
Cuts at: 0 1387 2214
Size: 1387 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1515 1547 2214
Size: 1515 32 667
Fragments arranged by size:
1515 667 32
XbaI T'CTAG_A
Cuts at: 0 1308 2214
Size: 1308 906
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1156 1434 2214
Size: 1156 278 780
Fragments arranged by size:
1156 780 278
XmnI GAAnn'nnTTC
Cuts at: 0 425 1340 2214
Size: 425 915 874
Fragments arranged by size:
915 874 425
ZraI GAC'GTC
Cuts at: 0 39 2214
Size: 39 2175
Enzymes that do cut:
AarI AatII AclI AcuI AflII AloI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BclI BfrBI
BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII HincII HindIII HpaI KasI MfeI MmeI
MscI MspA1I NarI NcoI NheI NsiI PflMI PfoI
PpiI PpuMI PvuII SacI SapI SfcI SfoI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AfeI AflIII AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I KpnI MluI MslI NaeI NdeI NgoMIV
NotI NruI NspI PacI PasI PciI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI