(Circular) (Six-base) MAPSORT of: pFN21AB3567.seq Check: 122 from: 1 to: 6847 pFN21AB3567 6847 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2836 2836 Size: 6847 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4657 278 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3339 4014 4190 1937 Size: 1402 675 176 4594 Fragments arranged by size: 4594 1402 675 176 Acc65I G'GTAC_C Cuts at: 2194 4168 2194 Size: 1974 4873 AclI AA'CG_TT Cuts at: 1563 4974 5347 1563 Size: 3411 373 3063 Fragments arranged by size: 3411 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3640 3742 4910 6294 6562 1632 Size: 2008 102 1168 1384 268 1917 Fragments arranged by size: 2008 1917 1384 1168 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6650 AflIII A'CryG_T Cuts at: 1203 5746 6723 1203 Size: 4543 977 1327 Fragments arranged by size: 4543 1327 977 AgeI A'CCGG_T Cuts at: 5678 5678 Size: 6847 AhdI GACnn_n'nnGTC Cuts at: 3244 5576 3244 Size: 2332 4515 AleI CACnn'nnGTG Cuts at: 1166 2027 2042 1166 Size: 861 15 5971 Fragments arranged by size: 5971 861 15 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3278 1184 Size: 2094 4753 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 3019 2987 Size: 32 6815 AlwNI CAG_nnn'CTG Cuts at: 2206 4590 6162 2206 Size: 2384 1572 2891 Fragments arranged by size: 2891 2384 1572 ApaLI G'TGCA_C Cuts at: 2537 4904 6060 2537 Size: 2367 1156 3324 Fragments arranged by size: 3324 2367 1156 ApoI r'AATT_y Cuts at: 1493 4156 4382 4534 6643 1493 Size: 2663 226 152 2109 1697 Fragments arranged by size: 2663 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5401 160 Size: 872 4369 1606 Fragments arranged by size: 4369 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 AvaI C'yCGr_G Cuts at: 1870 1943 3414 3476 4172 1870 Size: 73 1471 62 696 4545 Fragments arranged by size: 4545 1471 696 73 62 BamHI G'GATC_C Cuts at: 4177 4544 4177 Size: 367 6480 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 3122 3493 4168 5623 Size: 325 217 627 407 928 371 675 1455 Cuts at: 5623 618 Size: 1842 Fragments arranged by size: 1842 1455 928 675 627 407 371 325 217 BanII G_rGCy'C Cuts at: 1391 2741 3513 3978 4002 4166 1391 Size: 1350 772 465 24 164 4072 Fragments arranged by size: 4072 1350 772 465 164 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5876 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5038 5072 1903 Size: 34 3101 34 3678 Fragments arranged by size: 3678 3101 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4739 5949 4739 Size: 1210 5637 BclI T'GATC_A Cuts at: 1548 1548 Size: 6847 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3306 5458 136 Size: 107 122 71 631 605 1634 2152 1525 Fragments arranged by size: 2152 1634 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3051 4087 6842 3051 Size: 1036 2755 3056 Fragments arranged by size: 3056 2755 1036 BlpI GC'TnA_GC Cuts at: 4264 4264 Size: 6847 Bme1580I G_kGCm'C Cuts at: 1790 2541 3498 4908 6064 1790 Size: 751 957 1410 1156 2573 Fragments arranged by size: 2573 1410 1156 957 751 BmgBI CAC'GTC Cuts at: 1318 2488 1318 Size: 1170 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2049 2284 2348 Size: 416 53 83 399 24 632 235 64 Cuts at: 2348 2691 4578 5536 442 Size: 343 1887 958 1753 Fragments arranged by size: 1887 1753 958 632 416 399 343 235 83 64 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6847 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2755 2755 Size: 6847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 2750 2752 2944 3068 5507 1239 Size: 1345 166 2 192 124 2439 2579 Fragments arranged by size: 2579 2439 1345 192 166 124 2 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2597 2705 3062 4972 5837 6135 2597 Size: 108 357 1910 865 298 3309 Fragments arranged by size: 3309 1910 865 357 298 108 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4046 5510 882 Size: 617 2547 1464 2219 Fragments arranged by size: 2547 2219 1464 617 BsaAI yAC'GTr Cuts at: 493 1204 4638 493 Size: 711 3434 2702 Fragments arranged by size: 3434 2702 711 BsaBI GATnn'nnATC Cuts at: 6841 6841 Size: 6847 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3072 4654 5036 5671 Size: 53 83 186 936 1539 1582 382 635 Cuts at: 5671 275 Size: 1451 Fragments arranged by size: 1582 1539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 3352 3855 3876 5280 5678 5952 6099 6510 Size: 472 503 21 1404 398 274 147 411 Cuts at: 6510 2880 Size: 3217 Fragments arranged by size: 3217 1404 503 472 411 398 274 147 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6622 Fragments arranged by size: 6622 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2926 3641 1186 Size: 841 899 715 4392 Fragments arranged by size: 4392 899 841 715 BseYI C'CCAG_C Cuts at: 2080 2746 3166 3560 3817 6050 6546 2080 Size: 666 420 394 257 2233 496 2381 Fragments arranged by size: 2381 2233 666 496 420 394 257 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2020 2078 2381 1163 Size: 573 284 58 303 5629 Fragments arranged by size: 5629 573 303 284 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 3375 4014 4297 5058 6086 Size: 275 186 1309 65 639 283 761 1028 Cuts at: 6086 1540 Size: 2301 Fragments arranged by size: 2301 1309 1028 761 639 283 275 186 65 BsiHKAI G_wGCw'C Cuts at: 2338 2541 3143 3267 3618 3978 4908 4993 6064 Size: 203 602 124 351 360 930 85 1071 Cuts at: 6064 2338 Size: 3121 Fragments arranged by size: 3121 1071 930 602 360 351 203 124 85 BsmI GAATG_Cn' Cuts at: 2107 4358 4451 2107 Size: 2251 93 4503 Fragments arranged by size: 4503 2251 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3206 3417 3206 Size: 211 6636 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2338 2541 2741 3143 3267 3498 3513 Size: 399 548 203 200 402 124 231 15 Cuts at: 3513 3618 3978 4002 4166 4908 4993 6064 1391 Size: 105 360 24 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 548 402 399 360 231 203 200 164 124 105 85 24 15 BspEI T'CCGG_A Cuts at: 2880 3352 3855 3876 6510 2880 Size: 472 503 21 2634 3217 Fragments arranged by size: 3217 2634 503 472 21 BspHI T'CATG_A Cuts at: 4736 6466 4736 Size: 1730 5117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2511 2836 3450 3750 4202 844 Size: 1667 325 614 300 452 3489 Fragments arranged by size: 3489 1667 614 452 325 300 BsrBI CCG'CTC Cuts at: 1394 1823 2734 4734 1394 Size: 429 911 2000 3507 Fragments arranged by size: 3507 2000 911 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2364 3814 5342 5516 65 Size: 1183 1116 1450 1528 174 1396 Fragments arranged by size: 1528 1450 1396 1183 1116 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2026 2441 3370 5491 5678 1700 Size: 110 216 415 929 2121 187 2869 Fragments arranged by size: 2869 2121 929 415 216 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6847 BssHII G'CGCG_C Cuts at: 1925 3419 1925 Size: 1494 5353 BssSI C'ACGA_G Cuts at: 4907 5919 4907 Size: 1012 5835 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6847 BstBI TT'CG_AA Cuts at: 5651 5651 Size: 6847 BstEII G'GTnAC_C Cuts at: 1184 2665 1184 Size: 1481 5366 BstXI CCAn_nnnn'nTGG Cuts at: 2080 2080 Size: 6847 BstYI r'GATC_y Cuts at: 1972 3051 3198 3585 4087 4177 4544 4929 4946 Size: 1079 147 387 502 90 367 385 17 Cuts at: 4946 6398 6484 6496 6842 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1079 502 387 385 367 346 147 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2268 3144 513 Size: 552 1203 876 4216 Fragments arranged by size: 4216 1203 876 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2161 487 Size: 683 56 935 5173 Fragments arranged by size: 5173 935 683 56 BtsI GCAGTG_nn' Cuts at: 4363 5157 5177 4363 Size: 794 20 6033 Fragments arranged by size: 6033 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6847 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6812 DraI TTT'AAA Cuts at: 4151 4151 Size: 6847 DraIII CAC_nnn'GTG Cuts at: 2404 2404 Size: 6847 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4010 5854 809 Size: 507 2694 1844 1802 Fragments arranged by size: 2694 1844 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2416 3307 Size: 54 1425 50 275 18 531 55 891 Cuts at: 3307 3372 3402 3930 4294 5183 6812 8 Size: 65 30 528 364 889 1629 43 Fragments arranged by size: 1629 1425 891 889 531 528 364 275 65 55 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 3372 4294 1537 Size: 275 1495 65 922 4090 Fragments arranged by size: 4090 1495 922 275 65 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 3548 3618 3659 4777 1353 Size: 821 1374 70 41 1118 3423 Fragments arranged by size: 3423 1374 1118 821 70 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3455 5434 5808 5954 387 Size: 1064 138 1866 1979 374 146 1280 Fragments arranged by size: 1979 1866 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3976 3976 Size: 6847 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2584 2750 2752 2944 3068 3640 3742 Size: 393 952 166 2 192 124 572 102 Cuts at: 3742 4910 5507 6294 6562 1239 Size: 1168 597 787 268 1524 Fragments arranged by size: 1524 1168 952 787 597 572 393 268 192 166 124 102 2 EcoNI CCTnn'n_nnAGG Cuts at: 2120 3446 2120 Size: 1326 5521 EcoO109I rG'GnC_Cy Cuts at: 2163 2218 2225 2614 3346 3649 2163 Size: 55 7 389 732 303 5361 Fragments arranged by size: 5361 732 389 303 55 7 EcoRI G'AATT_C Cuts at: 4156 4534 4156 Size: 378 6469 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3409 3670 3409 Size: 261 6586 FspI TGC'GCA Cuts at: 2687 5353 2687 Size: 2666 4181 HaeII r_GCGC'y Cuts at: 1678 2571 2729 3301 5994 1678 Size: 893 158 572 2693 2531 Fragments arranged by size: 2693 2531 893 572 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2635 2921 2953 3230 Size: 32 135 32 1031 32 286 32 277 Cuts at: 3230 3262 5488 5520 5562 5594 1373 Size: 32 2226 32 42 32 2626 Fragments arranged by size: 2626 2226 1031 286 277 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4015 4191 4437 669 Size: 988 281 2077 176 246 3079 Fragments arranged by size: 3079 2077 988 281 246 176 HindIII A'AGCT_T Cuts at: 748 748 Size: 6847 HpaI GTT'AAC Cuts at: 4437 4437 Size: 6847 KpnI G_GTAC'C Cuts at: 2198 4172 2198 Size: 1974 4873 MfeI C'AATT_G Cuts at: 4446 4446 Size: 6847 MluI A'CGCG_T Cuts at: 6723 6723 Size: 6847 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5961 6145 1305 Size: 684 3972 184 2007 Fragments arranged by size: 3972 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3404 3932 10 Size: 54 1425 343 1572 528 2925 Fragments arranged by size: 2925 1572 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2042 2100 4805 5164 5323 518 Size: 648 861 15 58 2705 359 159 2042 Fragments arranged by size: 2705 2042 861 648 359 159 58 15 MspA1I CmG'CkG Cuts at: 1606 2068 2137 2461 3146 3664 4132 4263 4940 Size: 462 69 324 685 518 468 131 677 Cuts at: 4940 6088 6333 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 685 677 518 468 462 324 245 131 69 NaeI GCC'GGC Cuts at: 2443 3372 2443 Size: 929 5918 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6295 NdeI CA'TA_TG Cuts at: 387 2962 387 Size: 2575 4272 NgoMIV G'CCGG_C Cuts at: 2441 3370 2441 Size: 929 5918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6847 NotI GC'GGCC_GC Cuts at: 4294 4294 Size: 6847 NspI r_CATG'y Cuts at: 1132 1229 4205 5750 1132 Size: 97 2976 1545 2229 Fragments arranged by size: 2976 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2895 1348 Size: 1547 5300 PciI A'CATG_T Cuts at: 5746 5746 Size: 6847 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 3177 1108 Size: 1098 971 4778 Fragments arranged by size: 4778 1098 971 PmeI GTTT'AAAC Cuts at: 4151 4151 Size: 6847 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 4916 4948 6429 6461 4043 Size: 32 841 32 1481 32 4429 Fragments arranged by size: 4429 1481 841 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2163 2218 2225 3346 2163 Size: 55 7 1121 5664 Fragments arranged by size: 5664 1121 55 7 PshAI GACnn'nnGTC Cuts at: 1613 2223 1613 Size: 610 6237 PsiI TTA'TAA Cuts at: 4417 4417 Size: 6847 PstI C_TGCA'G Cuts at: 830 2157 3257 4199 830 Size: 1327 1100 942 3478 Fragments arranged by size: 3478 1327 1100 942 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6847 PvuII CAG'CTG Cuts at: 2137 2461 3664 4132 2137 Size: 324 1203 468 4852 Fragments arranged by size: 4852 1203 468 324 SacI G_AGCT'C Cuts at: 3978 3978 Size: 6847 SacII CC_GC'GG Cuts at: 3147 3147 Size: 6847 SalI G'TCGA_C Cuts at: 1936 4013 4189 1936 Size: 2077 176 4594 Fragments arranged by size: 4594 2077 176 SanDI GG'GwC_CC Cuts at: 2163 2225 2163 Size: 62 6785 SbfI CC_TGCA'GG Cuts at: 4199 4199 Size: 6847 ScaI AGT'ACT Cuts at: 5095 6807 5095 Size: 1712 5135 SfcI C'TryA_G Cuts at: 826 1045 2153 3253 4195 5330 6011 6202 826 Size: 219 1108 1100 942 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 1100 942 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 SgrAI Cr'CCGG_yG Cuts at: 1700 2026 1700 Size: 326 6521 SmaI CCC'GGG Cuts at: 1872 3478 4174 1872 Size: 1606 696 4545 Fragments arranged by size: 4545 1606 696 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 2720 3077 4951 5852 6114 Size: 197 926 633 144 357 1874 901 262 Cuts at: 6114 820 Size: 1553 Fragments arranged by size: 1874 1553 926 901 633 357 262 197 144 SnaBI TAC'GTA Cuts at: 493 4638 493 Size: 4145 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6847 SphI G_CATG'C Cuts at: 1132 4205 1132 Size: 3073 3774 SspI AAT'ATT Cuts at: 5 52 4771 5 Size: 47 4719 2081 Fragments arranged by size: 4719 2081 47 StuI AGG'CCT Cuts at: 1517 2183 2585 1517 Size: 666 402 5779 Fragments arranged by size: 5779 666 402 StyI C'CwwG_G Cuts at: 513 1065 2592 3750 4286 513 Size: 552 1527 1158 536 3074 Fragments arranged by size: 3074 1527 1158 552 536 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2470 3169 4874 4891 5044 5229 5674 Size: 1255 890 699 1705 17 153 185 445 Cuts at: 5674 325 Size: 1498 Fragments arranged by size: 1705 1498 1255 890 699 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 5093 6805 Size: 274 80 33 51 1443 54 3062 1712 Cuts at: 6805 96 Size: 138 Fragments arranged by size: 3062 1712 1443 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6807 6839 6807 Size: 32 6815 Tth111I GACn'n_nGTC Cuts at: 1316 2489 1316 Size: 1173 5674 XbaI T'CTAG_A Cuts at: 2776 4183 2776 Size: 1407 5440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 3174 2113 Size: 1061 5786 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6847 XmaI C'CCGG_G Cuts at: 1870 3476 4172 1870 Size: 1606 696 4545 Fragments arranged by size: 4545 1606 696 XmnI GAAnn'nnTTC Cuts at: 4976 4976 Size: 6847 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4655 276 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI