(Circular) (Six-base) MAPSORT of: pFN21AB3476.seq Check: 117 from: 1 to: 6874 pFN21AB3476 6874 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4684 278 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4217 1937 Size: 2280 4594 Acc65I G'GTAC_C Cuts at: 3666 4195 3666 Size: 529 6345 AclI AA'CG_TT Cuts at: 1563 5001 5374 1563 Size: 3438 373 3063 Fragments arranged by size: 3438 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2016 4937 6321 6589 1632 Size: 384 2921 1384 268 1917 Fragments arranged by size: 2921 1917 1384 384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6677 AflIII A'CryG_T Cuts at: 1203 2171 2562 3189 5773 6750 1203 Size: 968 391 627 2584 977 1327 Fragments arranged by size: 2584 1327 977 968 627 391 AgeI A'CCGG_T Cuts at: 5705 5705 Size: 6874 AhdI GACnn_n'nnGTC Cuts at: 5603 5603 Size: 6874 AleI CACnn'nnGTG Cuts at: 1166 2472 3675 1166 Size: 1306 1203 4365 Fragments arranged by size: 4365 1306 1203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2490 1184 Size: 1306 5568 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3179 3211 3179 Size: 32 6842 AlwNI CAG_nnn'CTG Cuts at: 4617 6189 4617 Size: 1572 5302 ApaLI G'TGCA_C Cuts at: 4931 6087 4931 Size: 1156 5718 ApoI r'AATT_y Cuts at: 1493 2842 2892 3077 3515 3578 3938 4169 4183 Size: 1349 50 185 438 63 360 231 14 Cuts at: 4183 4409 4561 6670 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 438 360 231 226 185 152 63 50 14 AseI AT'TA_AT Cuts at: 160 1032 5428 160 Size: 872 4396 1606 Fragments arranged by size: 4396 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 AvaI C'yCGr_G Cuts at: 1870 1943 3160 3363 4199 1870 Size: 73 1217 203 836 4545 Fragments arranged by size: 4545 1217 836 203 73 BamHI G'GATC_C Cuts at: 3961 4204 4571 3961 Size: 243 367 6264 Fragments arranged by size: 6264 367 243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3666 4195 5650 618 Size: 325 217 627 1879 529 1455 1842 Fragments arranged by size: 1879 1842 1455 627 529 325 217 BanII G_rGCy'C Cuts at: 1391 4193 1391 Size: 2802 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5903 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5065 5099 1903 Size: 34 3128 34 3678 Fragments arranged by size: 3678 3128 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4766 5976 4766 Size: 1210 5664 BclI T'GATC_A Cuts at: 1548 3265 1548 Size: 1717 5157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3301 3847 5485 Size: 107 122 71 631 605 1629 546 1638 Cuts at: 5485 136 Size: 1525 Fragments arranged by size: 1638 1629 1525 631 605 546 122 107 71 BglII A'GATC_T Cuts at: 2779 6869 2779 Size: 4090 2784 BlpI GC'TnA_GC Cuts at: 4291 4291 Size: 6874 Bme1580I G_kGCm'C Cuts at: 1790 4935 6091 1790 Size: 3145 1156 2573 Fragments arranged by size: 3145 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2174 1318 Size: 856 6018 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2866 4605 5563 Size: 416 53 83 399 24 1449 1739 958 Cuts at: 5563 442 Size: 1753 Fragments arranged by size: 1753 1739 1449 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3308 1056 Size: 2252 4622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3619 3619 Size: 6874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2630 2870 3363 3627 5534 1239 Size: 903 488 240 493 264 1907 2579 Fragments arranged by size: 2579 1907 903 493 488 264 240 Bpu10I CC'TnA_GC Cuts at: 2451 2451 Size: 6874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2198 2414 4999 5864 6162 2198 Size: 216 2585 865 298 2910 Fragments arranged by size: 2910 2585 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5537 882 Size: 617 4038 2219 Fragments arranged by size: 4038 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2853 3455 4665 493 Size: 711 1649 602 1210 2702 Fragments arranged by size: 2702 1649 1210 711 602 BsaBI GATnn'nnATC Cuts at: 3809 3960 6868 3809 Size: 151 2908 3815 Fragments arranged by size: 3815 2908 151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4681 5063 5698 275 Size: 53 83 186 936 3148 382 635 1451 Fragments arranged by size: 3148 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5307 5705 5979 6126 6537 5307 Size: 398 274 147 411 5644 Fragments arranged by size: 5644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2603 2633 3179 3209 1178 Size: 30 165 30 1200 30 546 30 4843 Fragments arranged by size: 4843 1200 546 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2066 1186 Size: 841 39 5994 Fragments arranged by size: 5994 841 39 BseYI C'CCAG_C Cuts at: 6077 6573 6077 Size: 496 6378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4324 5085 6113 1540 Size: 275 186 2323 761 1028 2301 Fragments arranged by size: 2323 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2479 3349 4935 5020 6091 2479 Size: 870 1586 85 1071 3262 Fragments arranged by size: 3262 1586 1071 870 85 BsiWI C'GTAC_G Cuts at: 3455 3455 Size: 6874 BsmI GAATG_Cn' Cuts at: 4385 4478 4385 Size: 93 6781 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2479 3349 4193 4935 5020 6091 1391 Size: 399 689 870 844 742 85 1071 2174 Fragments arranged by size: 2174 1071 870 844 742 689 399 85 BspEI T'CCGG_A Cuts at: 6537 6537 Size: 6874 BspHI T'CATG_A Cuts at: 3010 4763 6493 3010 Size: 1753 1730 3391 Fragments arranged by size: 3391 1753 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4229 844 Size: 3385 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4123 4761 1394 Size: 429 2300 638 3507 Fragments arranged by size: 3507 2300 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2599 2694 5369 5543 65 Size: 1183 1351 95 2675 174 1396 Fragments arranged by size: 2675 1396 1351 1183 174 95 BsrFI r'CCGG_y Cuts at: 1700 1810 5518 5705 1700 Size: 110 3708 187 2869 Fragments arranged by size: 3708 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3971 96 Size: 3875 2999 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6874 BssSI C'ACGA_G Cuts at: 4934 5946 4934 Size: 1012 5862 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6874 BstBI TT'CG_AA Cuts at: 3991 5678 3991 Size: 1687 5187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6874 BstXI CCAn_nnnn'nTGG Cuts at: 3282 3691 3943 3282 Size: 409 252 6213 Fragments arranged by size: 6213 409 252 BstYI r'GATC_y Cuts at: 1972 2117 2779 2914 3961 4204 4571 4956 4973 Size: 145 662 135 1047 243 367 385 17 Cuts at: 4973 6425 6511 6523 6869 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1047 662 385 367 346 243 145 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2496 3408 4090 513 Size: 552 1431 912 682 3297 Fragments arranged by size: 3297 1431 912 682 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6135 Fragments arranged by size: 6135 683 56 BtsI GCAGTG_nn' Cuts at: 2463 3250 4390 5184 5204 2463 Size: 787 1140 794 20 4133 Fragments arranged by size: 4133 1140 794 787 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6874 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6839 DraI TTT'AAA Cuts at: 2829 3487 4178 2829 Size: 658 691 5525 Fragments arranged by size: 5525 691 658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4085 5881 809 Size: 507 2769 1796 1802 Fragments arranged by size: 2769 1802 1796 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 3272 4321 Size: 54 1425 50 275 18 513 929 1049 Cuts at: 4321 5210 6839 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1049 929 889 513 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4321 1537 Size: 275 2509 4090 Fragments arranged by size: 4090 2509 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 2608 4804 1353 Size: 717 538 2196 3423 Fragments arranged by size: 3423 2196 717 538 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2523 5461 5835 5981 387 Size: 1064 138 934 2938 374 146 1280 Fragments arranged by size: 2938 1280 1064 934 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2016 2142 2630 2870 3363 3627 4937 Size: 393 384 126 488 240 493 264 1310 Cuts at: 4937 5534 6321 6589 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1310 787 597 493 488 393 384 268 264 240 126 EcoO109I rG'GnC_Cy Cuts at: 2386 2792 3279 2386 Size: 406 487 5981 Fragments arranged by size: 5981 487 406 EcoRI G'AATT_C Cuts at: 3077 4183 4561 3077 Size: 1106 378 5390 Fragments arranged by size: 5390 1106 378 EcoRV GAT'ATC Cuts at: 3529 3529 Size: 6874 FspI TGC'GCA Cuts at: 5380 5380 Size: 6874 HaeII r_GCGC'y Cuts at: 1678 6021 1678 Size: 4343 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 3757 3789 5515 Size: 32 135 32 704 32 1449 32 1726 Cuts at: 5515 5547 5589 5621 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1726 1449 704 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4218 4464 669 Size: 988 281 2280 246 3079 Fragments arranged by size: 3079 2280 988 281 246 HindIII A'AGCT_T Cuts at: 748 2646 748 Size: 1898 4976 HpaI GTT'AAC Cuts at: 4464 4464 Size: 6874 KpnI G_GTAC'C Cuts at: 3670 4199 3670 Size: 529 6345 MfeI C'AATT_G Cuts at: 4473 4473 Size: 6874 MluI A'CGCG_T Cuts at: 6750 6750 Size: 6874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2768 3403 5988 6172 1305 Size: 1463 635 2585 184 2007 Fragments arranged by size: 2585 2007 1463 635 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5052 Fragments arranged by size: 5052 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2472 3675 3741 4832 5191 5350 518 Size: 648 1306 1203 66 1091 359 159 2042 Fragments arranged by size: 2042 1306 1203 1091 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 3691 4290 4967 6115 6360 1606 Size: 2085 599 677 1148 245 2120 Fragments arranged by size: 2120 2085 1148 677 599 245 NcoI C'CATG_G Cuts at: 513 1065 2496 3408 513 Size: 552 1431 912 3979 Fragments arranged by size: 3979 1431 912 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6874 NheI G'CTAG_C Cuts at: 1052 3304 1052 Size: 2252 4622 NotI GC'GGCC_GC Cuts at: 4321 4321 Size: 6874 NspI r_CATG'y Cuts at: 1132 1229 2566 3193 4232 5777 1132 Size: 97 1337 627 1039 1545 2229 Fragments arranged by size: 2229 1545 1337 1039 627 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6874 PciI A'CATG_T Cuts at: 2562 3189 5773 2562 Size: 627 2584 3663 Fragments arranged by size: 3663 2584 627 PflMI CCAn_nnn'nTGG Cuts at: 1108 2856 3868 1108 Size: 1748 1012 4114 Fragments arranged by size: 4114 1748 1012 PfoI T'CCnGG_A Cuts at: 3111 3111 Size: 6874 PmeI GTTT'AAAC Cuts at: 4178 4178 Size: 6874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3176 3179 3208 3211 4943 4975 6456 6488 3176 Size: 3 29 3 1732 32 1481 32 3562 Fragments arranged by size: 3562 1732 1481 32 32 29 3 3 PpuMI rG'GwC_Cy Cuts at: 2386 2792 3279 2386 Size: 406 487 5981 Fragments arranged by size: 5981 487 406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6874 PsiI TTA'TAA Cuts at: 3063 4444 3063 Size: 1381 5493 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2690 2722 3858 3890 2690 Size: 32 1136 32 5674 Fragments arranged by size: 5674 1136 32 32 PstI C_TGCA'G Cuts at: 830 4226 830 Size: 3396 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6874 PvuII CAG'CTG Cuts at: 3691 3691 Size: 6874 RsrII CG'GwC_CG Cuts at: 2531 2531 Size: 6874 SalI G'TCGA_C Cuts at: 1936 4216 1936 Size: 2280 4594 SbfI CC_TGCA'GG Cuts at: 4226 4226 Size: 6874 ScaI AGT'ACT Cuts at: 5122 6834 5122 Size: 1712 5162 SfcI C'TryA_G Cuts at: 826 1045 2758 3178 4127 4222 5357 6038 6229 Size: 219 1713 420 949 95 1135 681 191 Cuts at: 6229 826 Size: 1471 Fragments arranged by size: 1713 1471 1135 949 681 420 219 191 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6874 SmaI CCC'GGG Cuts at: 1872 3365 4201 1872 Size: 1493 836 4545 Fragments arranged by size: 4545 1493 836 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 2429 4978 5879 6141 820 Size: 197 926 234 252 2549 901 262 1553 Fragments arranged by size: 2549 1553 926 901 262 252 234 197 SnaBI TAC'GTA Cuts at: 493 3455 4665 493 Size: 2962 1210 2702 Fragments arranged by size: 2962 2702 1210 SpeI A'CTAG_T Cuts at: 152 152 Size: 6874 SphI G_CATG'C Cuts at: 1132 4232 1132 Size: 3100 3774 SspI AAT'ATT Cuts at: 5 52 4798 5 Size: 47 4746 2081 Fragments arranged by size: 4746 2081 47 StuI AGG'CCT Cuts at: 1517 2340 1517 Size: 823 6051 StyI C'CwwG_G Cuts at: 513 1065 2150 2418 2496 3275 3408 3520 4313 Size: 552 1085 268 78 779 133 112 793 Cuts at: 4313 513 Size: 3074 Fragments arranged by size: 3074 1085 793 779 552 268 133 112 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 4901 4918 5071 5256 5701 325 Size: 1255 897 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 3971 5120 Size: 274 80 33 51 1443 720 1274 1149 Cuts at: 5120 6832 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1274 1149 720 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2603 2635 2828 2860 6834 6866 2603 Size: 32 193 32 3974 32 2611 Fragments arranged by size: 3974 2611 193 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6874 XbaI T'CTAG_A Cuts at: 4210 4210 Size: 6874 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2894 2894 Size: 6874 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6874 XmaI C'CCGG_G Cuts at: 1870 3363 4199 1870 Size: 1493 836 4545 Fragments arranged by size: 4545 1493 836 XmnI GAAnn'nnTTC Cuts at: 3191 5003 3191 Size: 1812 5062 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4682 276 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI