Restriction map for
pFN21AB3476
(Linear) (Six-base) MAPSORT of: KIBB3476.seq Check: 2928 from: 1 to: 2169
KIBB3476.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
Acc65I G'GTAC_C
Cuts at: 0 1661 2169
Size: 1661 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 2169
Size: 11 2158
AflIII A'CryG_T
Cuts at: 0 166 557 1184 2169
Size: 166 391 627 985
Fragments arranged by size:
985 627 391 166
AleI CACnn'nnGTG
Cuts at: 0 467 1670 2169
Size: 467 1203 499
Fragments arranged by size:
1203 499 467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 485 2169
Size: 485 1684
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 2169
Size: 1174 32 963
Fragments arranged by size:
1174 963 32
ApoI r'AATT_y
Cuts at: 0 837 887 1072 1510 1573 1933 2164 2169
Size: 837 50 185 438 63 360 231 5
Fragments arranged by size:
837 438 360 231 185 63 50 5
AvaI C'yCGr_G
Cuts at: 0 1155 1358 2169
Size: 1155 203 811
Fragments arranged by size:
1155 811 203
BamHI G'GATC_C
Cuts at: 0 1956 2169
Size: 1956 213
BanI G'GyrC_C
Cuts at: 0 1661 2169
Size: 1661 508
BclI T'GATC_A
Cuts at: 0 1260 2169
Size: 1260 909
BglI GCCn_nnn'nGGC
Cuts at: 0 1296 1842 2169
Size: 1296 546 327
Fragments arranged by size:
1296 546 327
BglII A'GATC_T
Cuts at: 0 774 2169
Size: 774 1395
BmgBI CAC'GTC
Cuts at: 0 169 2169
Size: 169 2000
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 2169
Size: 861 1308
BmtI G_CTAG'C
Cuts at: 0 1303 2169
Size: 1303 866
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1614 2169
Size: 1614 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 625 865 1358 1622 2169
Size: 137 488 240 493 264 547
Fragments arranged by size:
547 493 488 264 240 137
Bpu10I CC'TnA_GC
Cuts at: 0 446 2169
Size: 446 1723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 409 2169
Size: 193 216 1760
Fragments arranged by size:
1760 216 193
BsaAI yAC'GTr
Cuts at: 0 848 1450 2169
Size: 848 602 719
Fragments arranged by size:
848 719 602
BsaBI GATnn'nnATC
Cuts at: 0 1804 1955 2169
Size: 1804 151 214
Fragments arranged by size:
1804 214 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 598 628 1174 1204 2169
Size: 598 30 546 30 965
Fragments arranged by size:
965 598 546 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 61 2169
Size: 22 39 2108
Fragments arranged by size:
2108 39 22
BsiHKAI G_wGCw'C
Cuts at: 0 474 1344 2169
Size: 474 870 825
Fragments arranged by size:
870 825 474
BsiWI C'GTAC_G
Cuts at: 0 1450 2169
Size: 1450 719
Bsp1286I G_dGCh'C
Cuts at: 0 474 1344 2169
Size: 474 870 825
Fragments arranged by size:
870 825 474
BspHI T'CATG_A
Cuts at: 0 1005 2169
Size: 1005 1164
BsrBI CCG'CTC
Cuts at: 0 2118 2169
Size: 2118 51
BsrDI GCAATG_nn'
Cuts at: 0 594 689 2169
Size: 594 95 1480
Fragments arranged by size:
1480 594 95
BsrGI T'GTAC_A
Cuts at: 0 1966 2169
Size: 1966 203
BstBI TT'CG_AA
Cuts at: 0 1986 2169
Size: 1986 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1277 1686 1938 2169
Size: 1277 409 252 231
Fragments arranged by size:
1277 409 252 231
BstYI r'GATC_y
Cuts at: 0 112 774 909 1956 2169
Size: 112 662 135 1047 213
Fragments arranged by size:
1047 662 213 135 112
BtgI C'CryG_G
Cuts at: 0 491 1403 2085 2169
Size: 491 912 682 84
Fragments arranged by size:
912 682 491 84
BtsI GCAGTG_nn'
Cuts at: 0 458 1245 2169
Size: 458 787 924
Fragments arranged by size:
924 787 458
DraI TTT'AAA
Cuts at: 0 824 1482 2169
Size: 824 658 687
Fragments arranged by size:
824 687 658
DrdI GACnn_nn'nnGTC
Cuts at: 0 2080 2169
Size: 2080 89
EaeI y'GGCC_r
Cuts at: 0 338 1267 2169
Size: 338 929 902
Fragments arranged by size:
929 902 338
EarI CTCTTCn'nnn_
Cuts at: 0 65 603 2169
Size: 65 538 1566
Fragments arranged by size:
1566 538 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 518 2169
Size: 518 1651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 137 625 865 1358 1622 2169
Size: 11 126 488 240 493 264 547
Fragments arranged by size:
547 493 488 264 240 126 11
EcoO109I rG'GnC_Cy
Cuts at: 0 381 787 1274 2169
Size: 381 406 487 895
Fragments arranged by size:
895 487 406 381
EcoRI G'AATT_C
Cuts at: 0 1072 2169
Size: 1072 1097
EcoRV GAT'ATC
Cuts at: 0 1524 2169
Size: 1524 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 1752 1784 2169
Size: 271 32 1449 32 385
Fragments arranged by size:
1449 385 271 32 32
HindIII A'AGCT_T
Cuts at: 0 641 2169
Size: 641 1528
KpnI G_GTAC'C
Cuts at: 0 1665 2169
Size: 1665 504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1398 2169
Size: 763 635 771
Fragments arranged by size:
771 763 635
MslI CAynn'nnrTG
Cuts at: 0 467 1670 1736 2169
Size: 467 1203 66 433
Fragments arranged by size:
1203 467 433 66
MspA1I CmG'CkG
Cuts at: 0 1686 2169
Size: 1686 483
NcoI C'CATG_G
Cuts at: 0 491 1403 2169
Size: 491 912 766
Fragments arranged by size:
912 766 491
NheI G'CTAG_C
Cuts at: 0 1299 2169
Size: 1299 870
NspI r_CATG'y
Cuts at: 0 561 1188 2169
Size: 561 627 981
Fragments arranged by size:
981 627 561
PciI A'CATG_T
Cuts at: 0 557 1184 2169
Size: 557 627 985
Fragments arranged by size:
985 627 557
PflMI CCAn_nnn'nTGG
Cuts at: 0 851 1863 2169
Size: 851 1012 306
Fragments arranged by size:
1012 851 306
PfoI T'CCnGG_A
Cuts at: 0 1106 2169
Size: 1106 1063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1171 1174 1203 1206 2169
Size: 1171 3 29 3 963
Fragments arranged by size:
1171 963 29 3 3
PpuMI rG'GwC_Cy
Cuts at: 0 381 787 1274 2169
Size: 381 406 487 895
Fragments arranged by size:
895 487 406 381
PsiI TTA'TAA
Cuts at: 0 1058 2169
Size: 1058 1111
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 685 717 1853 1885 2169
Size: 685 32 1136 32 284
Fragments arranged by size:
1136 685 284 32 32
PvuII CAG'CTG
Cuts at: 0 1686 2169
Size: 1686 483
RsrII CG'GwC_CG
Cuts at: 0 526 2169
Size: 526 1643
SfcI C'TryA_G
Cuts at: 0 753 1173 2122 2169
Size: 753 420 949 47
Fragments arranged by size:
949 753 420 47
SmaI CCC'GGG
Cuts at: 0 1360 2169
Size: 1360 809
SmlI C'TyrA_G
Cuts at: 0 172 424 2169
Size: 172 252 1745
Fragments arranged by size:
1745 252 172
SnaBI TAC'GTA
Cuts at: 0 1450 2169
Size: 1450 719
StuI AGG'CCT
Cuts at: 0 335 2169
Size: 335 1834
StyI C'CwwG_G
Cuts at: 0 145 413 491 1270 1403 1515 2169
Size: 145 268 78 779 133 112 654
Fragments arranged by size:
779 654 268 145 133 112 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 2169
Size: 472 1697
TatI w'GTAC_w
Cuts at: 0 692 1966 2169
Size: 692 1274 203
Fragments arranged by size:
1274 692 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 598 630 823 855 2169
Size: 598 32 193 32 1314
Fragments arranged by size:
1314 598 193 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 889 2169
Size: 889 1280
XmaI C'CCGG_G
Cuts at: 0 1358 2169
Size: 1358 811
XmnI GAAnn'nnTTC
Cuts at: 0 1186 2169
Size: 1186 983
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AloI ApoI AvaI
BamHI BanI BclI BglI BglII BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI
BsiHKAI BsiWI Bsp1286I BspHI BsrBI BsrDI BsrGI BstBI
BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII KpnI
MmeI MslI MspA1I NcoI NheI NspI PciI PflMI
PfoI PpiI PpuMI PsiI PsrI PvuII RsrII SfcI
SmaI SmlI SnaBI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BlpI
Bme1580I BsaI BsaHI BsaWI BseYI BsgI BsiEI BsmI
BsmBI BspEI BspMI BsrFI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EcoICRI
EcoNI FalI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PmeI PmlI PshAI
PspOMI PspXI PstI PvuI SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI