(Circular) (Six-base) MAPSORT of: pFN21AB3373.seq Check: 2372 from: 1 to: 6562 pFN21AB3373 6562 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2068 3114 3905 1937 Size: 131 1046 791 4594 Fragments arranged by size: 4594 1046 791 131 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 4689 5062 1563 Size: 3126 373 3063 Fragments arranged by size: 3126 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3512 3615 3804 4625 6009 6277 1632 Size: 527 1353 103 189 821 1384 268 1917 Fragments arranged by size: 1917 1384 1353 821 527 268 189 103 AflII C'TTAA_G Cuts at: 820 1017 2926 3835 820 Size: 197 1909 909 3547 Fragments arranged by size: 3547 1909 909 197 AflIII A'CryG_T Cuts at: 1203 3843 5461 6438 1203 Size: 2640 1618 977 1327 Fragments arranged by size: 2640 1618 1327 977 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 3537 5291 3537 Size: 1754 4808 AleI CACnn'nnGTG Cuts at: 1166 3214 1166 Size: 2048 4514 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6562 AlwNI CAG_nnn'CTG Cuts at: 3232 3406 4305 5877 3232 Size: 174 899 1572 3917 Fragments arranged by size: 3917 1572 899 174 ApaLI G'TGCA_C Cuts at: 4619 5775 4619 Size: 1156 5406 ApoI r'AATT_y Cuts at: 1493 2144 2635 2741 2774 3222 3692 3871 4097 Size: 651 491 106 33 448 470 179 226 Cuts at: 4097 4249 6358 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 651 491 470 448 226 179 152 106 33 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 3054 3774 3887 1870 Size: 73 1111 720 113 4545 Fragments arranged by size: 4545 1111 720 113 73 AvrII C'CTAG_G Cuts at: 2708 2708 Size: 6562 BamHI G'GATC_C Cuts at: 2608 3283 3892 4259 2608 Size: 675 609 367 4911 Fragments arranged by size: 4911 675 609 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3883 5338 618 Size: 325 217 627 2096 1455 1842 Fragments arranged by size: 2096 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2716 3061 3593 3881 1391 Size: 1325 345 532 288 4072 Fragments arranged by size: 4072 1325 532 345 288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4753 4787 1903 Size: 34 2816 34 3678 Fragments arranged by size: 3678 2816 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 2011 2917 1548 Size: 463 906 5193 Fragments arranged by size: 5193 906 463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5173 136 Size: 107 122 71 631 605 3501 1525 Fragments arranged by size: 3501 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2203 2210 6557 2203 Size: 7 4347 2208 Fragments arranged by size: 4347 2208 7 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 3575 4623 5779 1790 Size: 1785 1048 1156 2573 Fragments arranged by size: 2573 1785 1156 1048 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4293 5251 442 Size: 416 53 83 399 24 2876 958 1753 Fragments arranged by size: 2876 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6562 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3800 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3091 3428 3658 5222 1239 Size: 1852 337 230 1564 2579 Fragments arranged by size: 2579 1852 1564 337 230 Bpu10I CC'TnA_GC Cuts at: 2644 2644 Size: 6562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2274 2333 3233 4687 5552 5850 2274 Size: 59 900 1454 865 298 2986 Fragments arranged by size: 2986 1454 900 865 298 59 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2898 3182 3808 5225 882 Size: 617 1399 284 626 1417 2219 Fragments arranged by size: 2219 1417 1399 626 617 284 BsaAI yAC'GTr Cuts at: 493 1204 4353 493 Size: 711 3149 2702 Fragments arranged by size: 3149 2702 711 BsaBI GATnn'nnATC Cuts at: 2733 6556 2733 Size: 3823 2739 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4369 4751 5386 275 Size: 53 83 186 936 2836 382 635 1451 Fragments arranged by size: 2836 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4995 5393 5667 5814 6225 4995 Size: 398 274 147 411 5332 Fragments arranged by size: 5332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6337 Fragments arranged by size: 6337 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2172 2487 1186 Size: 986 315 5261 Fragments arranged by size: 5261 986 315 BseYI C'CCAG_C Cuts at: 3041 5765 6261 3041 Size: 2724 496 3342 Fragments arranged by size: 3342 2724 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5989 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4012 4773 5801 1540 Size: 275 186 2011 761 1028 2301 Fragments arranged by size: 2301 2011 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3061 4623 4708 5779 3061 Size: 1562 85 1071 3844 Fragments arranged by size: 3844 1562 1071 85 BsmI GAATG_Cn' Cuts at: 2709 4073 4166 2709 Size: 1364 93 5105 Fragments arranged by size: 5105 1364 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2716 3061 3575 3593 3881 4623 4708 Size: 399 926 345 514 18 288 742 85 Cuts at: 4708 5779 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 926 742 514 399 345 288 85 18 BspEI T'CCGG_A Cuts at: 6225 6225 Size: 6562 BspHI T'CATG_A Cuts at: 4451 6181 4451 Size: 1730 4832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3917 844 Size: 3073 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4449 1394 Size: 429 2626 3507 Fragments arranged by size: 3507 2626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2399 3708 5057 5231 65 Size: 1183 1151 1309 1349 174 1396 Fragments arranged by size: 1396 1349 1309 1183 1151 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5206 5393 1700 Size: 110 3396 187 2869 Fragments arranged by size: 3396 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 3707 794 Size: 2913 3649 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstXI CCAn_nnnn'nTGG Cuts at: 3313 3313 Size: 6562 BstYI r'GATC_y Cuts at: 1972 2203 2210 2245 2608 3283 3892 4259 4644 Size: 231 7 35 363 675 609 367 385 Cuts at: 4644 4661 6113 6199 6211 6557 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 675 609 385 367 363 346 231 86 35 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5071 Fragments arranged by size: 5071 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 4078 4872 4892 4078 Size: 794 20 5748 Fragments arranged by size: 5748 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 3718 3866 3718 Size: 148 6414 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5569 809 Size: 507 4253 1802 Fragments arranged by size: 4253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3091 4009 4898 Size: 54 1425 50 275 18 1261 918 889 Cuts at: 4898 6527 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1261 918 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4009 1537 Size: 275 2197 4090 Fragments arranged by size: 4090 2197 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 3136 4492 1353 Size: 983 800 1356 3423 Fragments arranged by size: 3423 1356 983 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5149 5523 5669 387 Size: 1064 138 3560 374 146 1280 Fragments arranged by size: 3560 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3059 3059 Size: 6562 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 3091 3428 3512 3615 3658 3804 Size: 393 527 932 337 84 103 43 146 Cuts at: 3804 4625 5222 6009 6277 1239 Size: 821 597 787 268 1524 Fragments arranged by size: 1524 932 821 787 597 527 393 337 268 146 103 84 43 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2502 3106 2057 Size: 445 604 5513 Fragments arranged by size: 5513 604 445 EcoO109I rG'GnC_Cy Cuts at: 2471 2871 2471 Size: 400 6162 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 EcoRV GAT'ATC Cuts at: 3454 3454 Size: 6562 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 3366 5709 1678 Size: 1688 2343 2531 Fragments arranged by size: 2531 2343 1688 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2600 2632 2893 Size: 32 135 32 623 32 373 32 261 Cuts at: 2893 2925 3523 3555 5203 5235 5277 5309 1373 Size: 32 598 32 1648 32 42 32 2626 Fragments arranged by size: 2626 1648 623 598 373 261 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2069 3906 4152 669 Size: 988 281 131 1837 246 3079 Fragments arranged by size: 3079 1837 988 281 246 131 HindIII A'AGCT_T Cuts at: 748 2019 748 Size: 1271 5291 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 4161 4161 Size: 6562 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3086 5676 5860 1305 Size: 1781 2590 184 2007 Fragments arranged by size: 2590 2007 1781 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3093 10 Size: 54 1425 343 1261 3479 Fragments arranged by size: 3479 1425 1261 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3214 3311 3848 4520 4879 5038 518 Size: 648 2048 97 537 672 359 159 2042 Fragments arranged by size: 2048 2042 672 648 537 359 159 97 MspA1I CmG'CkG Cuts at: 1606 3824 3978 4655 5803 6048 1606 Size: 2218 154 677 1148 245 2120 Fragments arranged by size: 2218 2120 1148 677 245 154 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5071 Fragments arranged by size: 5071 939 552 NdeI CA'TA_TG Cuts at: 387 2337 3358 387 Size: 1950 1021 3591 Fragments arranged by size: 3591 1950 1021 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6562 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NspI r_CATG'y Cuts at: 1132 1229 3651 3847 3920 5465 1132 Size: 97 2422 196 73 1545 2229 Fragments arranged by size: 2422 2229 1545 196 97 73 PasI CC'CwG_GG Cuts at: 1348 2875 1348 Size: 1527 5035 PciI A'CATG_T Cuts at: 3843 5461 3843 Size: 1618 4944 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 1108 Size: 1497 5065 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2471 2871 2471 Size: 400 6162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PspXI vC'TCGA_Gb Cuts at: 3054 3054 Size: 6562 PstI C_TGCA'G Cuts at: 830 3914 830 Size: 3084 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 PvuII CAG'CTG Cuts at: 3824 3824 Size: 6562 SacI G_AGCT'C Cuts at: 3061 3061 Size: 6562 SalI G'TCGA_C Cuts at: 1936 2067 3904 1936 Size: 131 1837 4594 Fragments arranged by size: 4594 1837 131 SapI GCTCTTCn'nnn_ Cuts at: 2336 2336 Size: 6562 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 4810 6522 4810 Size: 1712 4850 SexAI A'CCwGG_T Cuts at: 2529 2529 Size: 6562 SfcI C'TryA_G Cuts at: 826 1045 3910 5045 5726 5917 826 Size: 219 2865 1135 681 191 1471 Fragments arranged by size: 2865 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3889 1872 Size: 2017 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2289 2348 2926 3054 3212 3774 Size: 197 926 346 59 578 128 158 562 Cuts at: 3774 3835 4666 5567 5829 820 Size: 61 831 901 262 1553 Fragments arranged by size: 1553 926 901 831 578 562 346 262 197 158 128 61 59 SnaBI TAC'GTA Cuts at: 493 4353 493 Size: 3860 2702 SpeI A'CTAG_T Cuts at: 152 2477 2555 2933 152 Size: 2325 78 378 3781 Fragments arranged by size: 3781 2325 378 78 SphI G_CATG'C Cuts at: 1132 3920 1132 Size: 2788 3774 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6562 StyI C'CwwG_G Cuts at: 513 1065 2004 2708 3094 3683 4001 513 Size: 552 939 704 386 589 318 3074 Fragments arranged by size: 3074 939 704 589 552 386 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3055 4589 4606 4759 4944 5389 325 Size: 1255 1475 1534 17 153 185 445 1498 Fragments arranged by size: 1534 1498 1475 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2375 3650 4808 Size: 274 80 33 51 1443 398 1275 1158 Cuts at: 4808 6520 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1275 1158 398 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6522 6554 6522 Size: 32 6530 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6562 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 3073 3101 2266 Size: 807 28 5727 Fragments arranged by size: 5727 807 28 XhoI C'TCGA_G Cuts at: 1943 3054 3774 1943 Size: 1111 720 4731 Fragments arranged by size: 4731 1111 720 XmaI C'CCGG_G Cuts at: 1870 3887 1870 Size: 2017 4545 XmnI GAAnn'nnTTC Cuts at: 4691 4691 Size: 6562 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI