Restriction map for
pFN21AB3347
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AccIII AcsI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI Bst1107I BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI FspI HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 1240 GACGTC GACGT^C
AccII 496 CGCG CG^CG
AclI 117 AACGTT AA^CGTT
AcyI 1240 GACGTC G[GA]^CG[CT]C
AflII 27 CTTAAG C^TTAAG
AocI 1138 CCTCAGG CC^T[ACGT]AGG
ApaI 860 GGGCCC GGGCC^C
AspI 868 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1574 GGACC G^G[AT]CC
BalI 1286 TGGCCA TGG^CCA
BciVI 1557 GGATAC GTATCC(6/5)
BclI 941 TGATCA T^GATCA
BfrI 27 CTTAAG C^TTAAG
BsaHI 1240 GACGTC G[GA]^CG[CT]C
BsaWI 1026 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1982 TGTACA T^GTACA
BspHI 2205 TCATGA T^CATGA
BsrFI 32 GCCGGC [GA]^CCGG[CT]
BsrGI 1982 TGTACA T^GTACA
BssHII 495 GCGCGC G^CGCGC
BssSI 1563 CACGAG CACGAG(-5/-1)
BstAPI 94 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 496 CGCG CG^CG
Bsu36I 1138 CCTCAGG CC^T[ACGT]AGG
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
DraII 859 AGGGCCC [GA]G^G[ACGT]CC[CT]
Eco81I 1138 CCTCAGG CC^T[ACGT]AGG
EcoNI 256 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 859 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1842 ATGCAT ATGCA^T
FbaI 941 TGATCA T^GATCA
HaeII 704 AGCGCC [GA]GCGC^[CT]
HgaI 1221 GCGTC GACGC(5/10)
Hin1I 1240 GACGTC G[GA]^CG[CT]C
HincII 55 GTTGAC GT[CT]^[GA]AC
HindII 55 GTTGAC GT[CT]^[GA]AC
HindIII 634 AAGCTT A^AGCTT
MluNI 1286 TGGCCA TGG^CCA
MscI 1286 TGGCCA TGG^CCA
MvnI 496 CGCG CG^CG
NaeI 32 GCCGGC GCC^GGC
NcoI 1005 CCATGG C^CATGG
NgoMIV 32 GCCGGC G^CCGGC
NsiI 1842 ATGCAT ATGCA^T
PaeR7I 932 CTCGAG C^TCGAG
PflFI 868 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 663 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 117 AACGTT AA^CGTT
RcaI 2205 TCATGA T^CATGA
SacI 929 GAGCTC GAGCT^C
SapI 1951 GAAGAGC GCTCTTC(1/4)
ScaI 1945 AGTACT AGT^ACT
SmaI 500 CCCGGG CCC^GGG
SspBI 1982 TGTACA T^GTACA
StuI 436 AGGCCT AGG^CCT
TaqI 933 TCGA T^CGA
Tth111I 868 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 933 TCGA T^CGA
Van91I 663 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 932 CTCGAG C^TCGAG
XmaCI 500 CCCGGG C^CCGGG
XmaI 500 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcnI 500 CCCGG CC^[GC]GG
501 CCGGG
BpmI 1156 CTCCAG CTGGAG(16/14)
727 CTGGAG
BsaBI 457 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2101 GATGTTGATC
BstEII 814 GGTAACC G^GT[ACGT]ACC
1938 GGTCACC
BstF5I 156 GGATG GGATG(2/0)
852 GGATG
BstPI 814 GGTAACC G^GT[ACGT]ACC
1938 GGTCACC
BstYI 845 AGATCC [GA]^GATC[CT]
1160 AGATCC
DrdI 1093 GACACTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1575 GACCAAGATGTC
DsaI 1005 CCATGG C^C[GA][CT]GG
1415 CCACGG
EcoO65I 814 GGTAACC G^GT[ACGT]ACC
1938 GGTCACC
EcoT14I 141 CCAAGG C^C[AT][AT]GG
1005 CCATGG
FokI 156 GGATG GGATG(9/13)
852 GGATG
MamI 457 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2101 GATGTTGATC
MflI 845 AGATCC [GA]^GATC[CT]
1160 AGATCC
NciI 500 CCCGG CC^[GC]GG
501 CCGGG
PstI 757 CTGCAG CTGCA^G
1225 CTGCAG
SfcI 757 CTGCAG C^T[GA][CT]AG
1225 CTGCAG
SmlI 27 CTTAAG C^T[CT][GA]AG
932 CTCGAG
SphI 180 GCATGC GCATG^C
833 GCATGC
StyI 141 CCAAGG C^C[AT][AT]GG
1005 CCATGG
Tsp45I 1740 GTCAC ^GT[GC]AC
1939 GTCAC
XhoII 845 AGATCC [GA]^GATC[CT]
1160 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI BanII BbsI BbvI
BfaI BmyI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI