(Circular) (Six-base) MAPSORT of: pFN21AB3201.seq Check: 1742 from: 1 to: 5743 pFN21AB3201 5743 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2770 3806 5190 5458 1632 Size: 1138 1036 1384 268 1917 Fragments arranged by size: 1917 1384 1138 1036 268 AflII C'TTAA_G Cuts at: 820 1017 2182 820 Size: 197 1165 4381 Fragments arranged by size: 4381 1165 197 AflIII A'CryG_T Cuts at: 1203 2355 2589 2721 4642 5619 1203 Size: 1152 234 132 1921 977 1327 Fragments arranged by size: 1921 1327 1152 977 234 132 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 2020 4472 2020 Size: 2452 3291 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2872 3486 5058 2872 Size: 614 1572 3557 Fragments arranged by size: 3557 1572 614 ApaI G_GGCC'C Cuts at: 2545 2545 Size: 5743 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2546 3068 1870 Size: 73 82 521 522 4545 Fragments arranged by size: 4545 522 521 82 73 BamHI G'GATC_C Cuts at: 2400 3073 3440 2400 Size: 673 367 4703 Fragments arranged by size: 4703 673 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 4519 618 Size: 325 217 627 1277 1455 1842 Fragments arranged by size: 1842 1455 1277 627 325 217 BanII G_rGCy'C Cuts at: 1391 2498 2545 3062 1391 Size: 1107 47 517 4072 Fragments arranged by size: 4072 1107 517 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2723 928 Size: 971 824 3948 Fragments arranged by size: 3948 971 824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 2662 1548 Size: 1114 4629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2867 4354 136 Size: 107 122 71 631 605 1195 1487 1525 Fragments arranged by size: 1525 1487 1195 631 605 122 107 71 BglII A'GATC_T Cuts at: 2812 5738 2812 Size: 2926 2817 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2545 2696 3804 4960 1790 Size: 755 151 1108 1156 2573 Fragments arranged by size: 2573 1156 1108 755 151 BmgBI CAC'GTC Cuts at: 1318 2017 1318 Size: 699 5044 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4403 1239 Size: 3164 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 4406 882 Size: 617 738 2169 2219 Fragments arranged by size: 2219 2169 738 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 1178 Size: 30 165 30 651 30 4837 Fragments arranged by size: 4837 651 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5743 BseYI C'CCAG_C Cuts at: 4946 5442 4946 Size: 496 5247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2811 1163 Size: 573 1075 4095 Fragments arranged by size: 4095 1075 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2851 3804 3889 4960 2851 Size: 953 85 1071 3634 Fragments arranged by size: 3634 1071 953 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 BsmBI CGTCTCn'nnnn_ Cuts at: 2793 2793 Size: 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2498 2545 2696 2851 3062 3804 3889 Size: 399 708 47 151 155 211 742 85 Cuts at: 3889 4960 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 708 399 211 155 151 85 47 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 844 Size: 2254 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2400 2413 2812 3073 3440 3825 3842 5294 Size: 428 13 399 261 367 385 17 1452 Cuts at: 5294 5380 5392 5738 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 428 399 385 367 346 261 86 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2937 4750 809 Size: 507 1621 1813 1802 Fragments arranged by size: 1813 1802 1621 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2478 2714 2859 Size: 54 1425 50 275 18 648 236 145 Cuts at: 2859 2897 3190 4079 5708 8 Size: 38 293 889 1629 43 Fragments arranged by size: 1629 1425 889 648 293 275 236 145 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2373 3673 1353 Size: 1020 1300 3423 Fragments arranged by size: 3423 1300 1020 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2770 3806 4403 5190 5458 1239 Size: 393 1138 1036 597 787 268 1524 Fragments arranged by size: 1524 1138 1036 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2060 Size: 5743 EcoO109I rG'GnC_Cy Cuts at: 2542 2542 Size: 5743 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2652 2068 Size: 584 5159 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 4890 1678 Size: 3212 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 4384 4416 4458 Size: 32 135 32 434 32 2346 32 42 Cuts at: 4458 4490 1373 Size: 32 2626 Fragments arranged by size: 2626 2346 434 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2480 10 Size: 54 1425 343 648 3273 Fragments arranged by size: 3273 1425 648 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2914 3701 4060 4219 518 Size: 648 1194 554 787 359 159 2042 Fragments arranged by size: 2042 1194 787 648 554 359 159 MspA1I CmG'CkG Cuts at: 1606 2923 3159 3836 4984 5229 1606 Size: 1317 236 677 1148 245 2120 Fragments arranged by size: 2120 1317 1148 677 245 236 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2359 2593 2725 3101 4646 1132 Size: 97 1130 234 132 376 1545 2229 Fragments arranged by size: 2229 1545 1130 376 234 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 2355 2589 2721 4642 2355 Size: 234 132 1921 3456 Fragments arranged by size: 3456 1921 234 132 PflMI CCAn_nnn'nTGG Cuts at: 1108 2912 1108 Size: 1804 3939 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 2306 3313 2306 Size: 1007 4736 PspOMI G'GGCC_C Cuts at: 2541 2541 Size: 5743 PstI C_TGCA'G Cuts at: 830 3095 830 Size: 2265 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2923 2923 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SexAI A'CCwGG_T Cuts at: 2528 2528 Size: 5743 SfcI C'TryA_G Cuts at: 826 1045 3091 4226 4907 5098 826 Size: 219 2046 1135 681 191 1471 Fragments arranged by size: 2046 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2182 3847 4748 5010 820 Size: 197 926 239 1665 901 262 1553 Fragments arranged by size: 1665 1553 926 901 262 239 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 2263 2384 2679 3667 5 Size: 47 2211 121 295 988 2081 Fragments arranged by size: 2211 2081 988 295 121 47 StuI AGG'CCT Cuts at: 1517 3005 1517 Size: 1488 4255 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 3182 513 Size: 552 939 52 1126 3074 Fragments arranged by size: 3074 1126 939 552 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3989 5701 96 Size: 274 80 33 51 1443 2012 1712 138 Fragments arranged by size: 2012 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2560 2592 5703 5735 2560 Size: 32 3111 32 2568 Fragments arranged by size: 3111 2568 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 2296 3079 2296 Size: 783 4960 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 2885 3872 2885 Size: 987 4756 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI