################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SapI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 148 GTCGAC GT^[AC][GT]AC AciI 175 GCGG CCGC(-3/-1) AflIII 992 ACATGT A^C[GA][CT]GT AlwI 951 GGATC GGATC(4/5) AocI 334 CCTGAGG CC^T[ACGT]AGG Asp700I 1491 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 238 GAGCTC G[AT]GC[AT]^C BanII 238 GAGCTC G[GA]GC[CT]^C BbrPI 874 CACGTG CAC^GTG BcgI 486 CGATGTATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 225 GGATAC GTATCC(6/5) BcnI 279 CCGGG CC^[GC]GG BmyI 238 GAGCTC G[AGT]GC[ACT]^C BpmI 945 CTGGAG CTGGAG(16/14) BsaAI 874 CACGTG [CT]AC^GT[GA] BseRI 1156 GAGGAG GAGGAG(10/8) BsiHKAI 238 GAGCTC G[AT]GC[AT]^C BsmAI 1624 GAGAC GTCTC(1/5) Bsp1286I 238 GAGCTC G[AGT]GC[ACT]^C BspHI 774 TCATGA T^CATGA BspLU11I 992 ACATGT A^CATGT BstXI 1382 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 951 GGATCT [GA]^GATC[CT] Bsu36I 334 CCTGAGG CC^T[ACGT]AGG CfoI 87 GCGC GCG^C DraII 1450 GGGTCCT [GA]G^G[ACGT]CC[CT] Eco81I 334 CCTGAGG CC^T[ACGT]AGG EcoNI 1149 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1450 GGGTCCT [GA]G^G[ACGT]CC[CT] HaeII 86 AGCGCC [GA]GCGC^[CT] HapII 279 CCGG C^CGG HhaI 87 GCGC GCG^C HinP1I 87 GCGC G^CGC HindIII 614 AAGCTT A^AGCTT HpaI 139 GTTAAC GTT^AAC HpaII 279 CCGG C^CGG MflI 951 GGATCT [GA]^GATC[CT] MspI 279 CCGG C^CGG NciI 279 CCGGG CC^[GC]GG NdeI 418 CATATG CA^TATG PflMI 1112 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 874 CACGTG CAC^GTG PmlI 874 CACGTG CAC^GTG PpuMI 1450 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1168 CTGCAG CTGCA^G RcaI 774 TCATGA T^CATGA SacI 238 GAGCTC GAGCT^C SalI 148 GTCGAC G^TCGAC SbfI 1167 CCTGCAGG CCTGCA^GG SexAI 1508 ACCTGGT A^CC[AT]GGT SmlI 862 CTTGAG C^T[CT][GA]AG SphI 366 GCATGC GCATG^C Sse8387I 1167 CCTGCAGG CCTGCA^GG SspI 163 AATATT AAT^ATT Van91I 1112 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 951 GGATCT [GA]^GATC[CT] XmnI 1491 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 402 GTAC GT^AC 639 GTAC AlwNI 85 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 440 CAGCAGCTG AspI 259 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 819 GACAAGGTC BbsI 215 GAAGAC GAAGAC(2/6) 1591 GAAGAC BfaI 8 CTAG C^TAG 964 CTAG BpuAI 215 GAAGAC GAAGAC(2/6) 1591 GAAGAC BsaJI 1142 CCATGG C^C[ACGT][ACGT]GG 1232 CCATGG BsgI 1556 CTGCAC GTGCAG(16/14) 881 GTGCAG BspDI 202 ATCGAT AT^CGAT 1261 ATCGAT BsrDI 1401 CATTGC GCAATG(2/0) 751 GCAATG BstF5I 317 GGATG GGATG(2/0) 465 GGATG BstNI 1118 CCTGG CC^[AT]GG 1509 CCTGG Cac8I 366 GCATGC GC[ACGT]^[ACGT]GC 1369 GCAAGC ClaI 202 ATCGAT AT^CGAT 1261 ATCGAT DsaI 1142 CCATGG C^C[GA][CT]GG 1232 CCATGG EarI 1289 CTCTTC CTCTTC(1/4) 1491 GAAGAG EcoRII 1118 CCTGG ^CC[AT]GG 1509 CCTGG EcoT14I 1142 CCATGG C^C[AT][AT]GG 1232 CCATGG FokI 317 GGATG GGATG(9/13) 465 GGATG HaeIII 1146 GGCC GG^CC 1239 GGCC Ksp632I 1289 CTCTTC CTCTTC(1/4) 1491 GAAGAG MaeI 8 CTAG C^TAG 964 CTAG MaeII 143 ACGT A^CGT 875 ACGT MvaI 1118 CCTGG CC^[AT]GG 1509 CCTGG NcoI 1142 CCATGG C^CATGG 1232 CCATGG NspI 366 GCATGC [GA]CATG^[CT] 992 ACATGT PflFI 259 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 819 GACAAGGTC PleI 259 GACTC GAGTC(4/5) 1300 GACTC RsaI 402 GTAC GT^AC 639 GTAC SfcI 986 CTACAG C^T[GA][CT]AG 1168 CTGCAG StyI 1142 CCATGG C^C[AT][AT]GG 1232 CCATGG Tth111I 259 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 819 GACAAGGTC XcmI 1108 CCAGCCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1142 CCATGGCCCTAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AluI ApoI AvaII BbvI BsiYI BslI BsmFI BsrI BssKI Cfr13I DdeI DpnII DraIII Fnu4HI HincII HindII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I