(Circular) (Six-base) MAPSORT of: pFN21AB3114.seq Check: 9115 from: 1 to: 6340 pFN21AB3114 6340 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3683 1937 Size: 217 1529 4594 Fragments arranged by size: 4594 1529 217 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 2298 2552 3072 3515 3633 4403 5787 Size: 624 42 254 520 443 118 770 1384 Cuts at: 5787 6055 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 770 624 520 443 268 254 118 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 2997 5239 6216 1203 Size: 1794 2242 977 1327 Fragments arranged by size: 2242 1794 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 2883 3392 1166 Size: 1717 509 4114 Fragments arranged by size: 4114 1717 509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6340 AlwNI CAG_nnn'CTG Cuts at: 2095 2450 4083 5655 2095 Size: 355 1633 1572 2780 Fragments arranged by size: 2780 1633 1572 355 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 2354 2388 2748 2932 3649 3875 4027 6136 Size: 861 34 360 184 717 226 152 2109 Cuts at: 6136 1493 Size: 1697 Fragments arranged by size: 2109 1697 861 717 360 226 184 152 34 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 3665 1870 Size: 73 1722 4545 Fragments arranged by size: 4545 1722 73 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3661 5116 618 Size: 325 217 627 1874 1455 1842 Fragments arranged by size: 1874 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2247 3659 1391 Size: 856 1412 4072 Fragments arranged by size: 4072 1412 856 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 3603 928 Size: 971 328 1376 3665 Fragments arranged by size: 3665 1376 971 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2480 2514 4531 4565 1903 Size: 34 543 34 2017 34 3678 Fragments arranged by size: 3678 2017 543 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2224 4232 5442 2224 Size: 2008 1210 3122 Fragments arranged by size: 3122 2008 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4951 136 Size: 107 122 71 631 605 3279 1525 Fragments arranged by size: 3279 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 4401 5557 1790 Size: 2611 1156 2573 Fragments arranged by size: 2611 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3241 4071 5029 Size: 416 53 83 399 24 1824 830 958 Cuts at: 5029 442 Size: 1753 Fragments arranged by size: 1824 1753 958 830 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3361 3361 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2971 5000 1239 Size: 1732 2029 2579 Fragments arranged by size: 2579 2029 1732 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2888 4465 5330 5628 2888 Size: 1577 865 298 3600 Fragments arranged by size: 3600 1577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5003 882 Size: 617 3504 2219 Fragments arranged by size: 3504 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2881 4131 493 Size: 711 1677 1250 2702 Fragments arranged by size: 2702 1677 1250 711 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4147 4529 5164 275 Size: 53 83 186 936 2614 382 635 1451 Fragments arranged by size: 2614 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6115 Fragments arranged by size: 6115 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3176 1186 Size: 1990 4350 BseYI C'CCAG_C Cuts at: 3112 5543 6039 3112 Size: 2431 496 3413 Fragments arranged by size: 3413 2431 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2907 3546 1163 Size: 573 1171 639 3957 Fragments arranged by size: 3957 1171 639 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 4551 5579 1540 Size: 275 186 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 4401 4486 5557 2247 Size: 2154 85 1071 3030 Fragments arranged by size: 3030 2154 1071 85 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 3659 4401 4486 5557 1391 Size: 399 457 1412 742 85 1071 2174 Fragments arranged by size: 2174 1412 1071 742 457 399 85 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 2779 4229 5959 2779 Size: 1450 1730 3160 Fragments arranged by size: 3160 1730 1450 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3695 844 Size: 2851 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4227 1394 Size: 429 2404 3507 Fragments arranged by size: 3507 2404 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2763 3405 4835 5009 65 Size: 1183 1515 642 1430 174 1396 Fragments arranged by size: 1515 1430 1396 1183 642 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4984 5171 1700 Size: 110 3174 187 2869 Fragments arranged by size: 3174 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6340 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6340 BstXI CCAn_nnnn'nTGG Cuts at: 3394 3394 Size: 6340 BstYI r'GATC_y Cuts at: 1972 2956 3670 4037 4422 4439 5891 5977 5989 Size: 984 714 367 385 17 1452 86 12 Cuts at: 5989 6335 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 984 714 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2340 2340 Size: 6340 BtgI C'CryG_G Cuts at: 513 1065 2004 3147 3237 513 Size: 552 939 1143 90 3616 Fragments arranged by size: 3616 1143 939 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 3492 3856 4650 4670 3492 Size: 364 794 20 5162 Fragments arranged by size: 5162 794 364 20 ClaI AT'CG_AT Cuts at: 1554 2208 3267 1554 Size: 654 1059 4627 Fragments arranged by size: 4627 1059 654 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2008 2043 3222 3257 529 Size: 35 1444 35 1179 35 3612 Fragments arranged by size: 3612 1444 1179 35 35 35 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DraIII CAC_nnn'GTG Cuts at: 2881 3221 3569 2881 Size: 340 348 5652 Fragments arranged by size: 5652 348 340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3787 4676 6305 Size: 54 1425 50 275 18 1957 889 1629 Cuts at: 6305 8 Size: 43 Fragments arranged by size: 1957 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 1537 Size: 275 1975 4090 Fragments arranged by size: 4090 1975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3300 3491 4270 1353 Size: 1947 191 779 3423 Fragments arranged by size: 3423 1947 779 191 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4927 5301 5447 387 Size: 1064 138 3338 374 146 1280 Fragments arranged by size: 3338 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 6340 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2298 2552 2971 3072 3515 3633 Size: 393 624 42 254 419 101 443 118 Cuts at: 3633 4403 5000 5787 6055 1239 Size: 770 597 787 268 1524 Fragments arranged by size: 1524 787 770 624 597 443 419 393 268 254 118 101 42 EcoNI CCTnn'n_nnAGG Cuts at: 3158 3158 Size: 6340 EcoO109I rG'GnC_Cy Cuts at: 3456 3456 Size: 6340 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2095 5487 1678 Size: 417 3392 2531 Fragments arranged by size: 3392 2531 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 3215 3247 4981 Size: 32 135 32 896 32 715 32 1734 Cuts at: 4981 5013 5055 5087 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1734 896 715 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 2155 3234 3684 3930 669 Size: 988 281 208 9 1079 450 246 3079 Fragments arranged by size: 3079 1079 988 450 281 246 208 9 HindIII A'AGCT_T Cuts at: 748 2619 748 Size: 1871 4469 HpaI GTT'AAC Cuts at: 2146 3930 2146 Size: 1784 4556 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4518 Fragments arranged by size: 4518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2784 2883 3392 4298 4657 4816 518 Size: 648 1618 99 509 906 359 159 2042 Fragments arranged by size: 2042 1618 906 648 509 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2128 2450 2678 3756 4433 5581 5826 1606 Size: 522 322 228 1078 677 1148 245 2120 Fragments arranged by size: 2120 1148 1078 677 522 322 245 228 NcoI C'CATG_G Cuts at: 513 1065 2004 3147 3237 513 Size: 552 939 1143 90 3616 Fragments arranged by size: 3616 1143 939 552 90 NdeI CA'TA_TG Cuts at: 387 2424 387 Size: 2037 4303 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2375 3001 3698 5243 1132 Size: 97 1146 626 697 1545 2229 Fragments arranged by size: 2229 1545 1146 697 626 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 2997 5239 2997 Size: 2242 4098 PflMI CCAn_nnn'nTGG Cuts at: 1108 3123 1108 Size: 2015 4325 PfoI T'CCnGG_A Cuts at: 2283 3122 2283 Size: 839 5501 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 2881 2881 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3456 3456 Size: 6340 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PstI C_TGCA'G Cuts at: 830 3177 3692 830 Size: 2347 515 3478 Fragments arranged by size: 3478 2347 515 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2128 2450 2678 2128 Size: 322 228 5790 Fragments arranged by size: 5790 322 228 SacI G_AGCT'C Cuts at: 2247 2247 Size: 6340 SalI G'TCGA_C Cuts at: 1936 2153 3682 1936 Size: 217 1529 4594 Fragments arranged by size: 4594 1529 217 SbfI CC_TGCA'GG Cuts at: 3177 3692 3177 Size: 515 5825 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SexAI A'CCwGG_T Cuts at: 3513 3513 Size: 6340 SfcI C'TryA_G Cuts at: 826 1045 2991 3173 3688 4823 5504 5695 826 Size: 219 1946 182 515 1135 681 191 1471 Fragments arranged by size: 1946 1471 1135 681 515 219 191 182 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 4444 5345 5607 820 Size: 197 926 924 1577 901 262 1553 Fragments arranged by size: 1577 1553 926 924 901 262 197 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 2375 3698 1132 Size: 1243 1323 3774 Fragments arranged by size: 3774 1323 1243 SspI AAT'ATT Cuts at: 5 52 2170 4264 5 Size: 47 2118 2094 2081 Fragments arranged by size: 2118 2094 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6340 StyI C'CwwG_G Cuts at: 513 1065 2004 3147 3237 3779 513 Size: 552 939 1143 90 542 3074 Fragments arranged by size: 3074 1143 939 552 542 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3126 4367 4384 4537 4722 5167 325 Size: 1255 1546 1241 17 153 185 445 1498 Fragments arranged by size: 1546 1498 1255 1241 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2643 4586 6298 Size: 274 80 33 51 1443 666 1943 1712 Cuts at: 6298 96 Size: 138 Fragments arranged by size: 1943 1712 1443 666 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2411 6300 6332 2379 Size: 32 3889 32 2387 Fragments arranged by size: 3889 2387 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2267 2827 1316 Size: 951 560 4829 Fragments arranged by size: 4829 951 560 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3120 3154 3120 Size: 34 6306 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 3500 4469 3500 Size: 969 5371 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI