(Circular) (Six-base) MAPSORT of: pFN21AB3063.seq Check: 2008 from: 1 to: 6850 pFN21AB3063 6850 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4660 278 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2934 4193 1937 Size: 997 1259 4594 Fragments arranged by size: 4594 1259 997 Acc65I G'GTAC_C Cuts at: 4171 4171 Size: 6850 AclI AA'CG_TT Cuts at: 1563 2306 4977 5350 1563 Size: 743 2671 373 3063 Fragments arranged by size: 3063 2671 743 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 2556 2957 3575 4913 6297 6565 1632 Size: 599 325 401 618 1338 1384 268 1917 Fragments arranged by size: 1917 1384 1338 618 599 401 325 268 AfeI AGC'GCT Cuts at: 3075 3243 3075 Size: 168 6682 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6653 AflIII A'CryG_T Cuts at: 1203 2090 3666 5749 6726 1203 Size: 887 1576 2083 977 1327 Fragments arranged by size: 2083 1576 1327 977 887 AgeI A'CCGG_T Cuts at: 5681 5681 Size: 6850 AhdI GACnn_n'nnGTC Cuts at: 5579 5579 Size: 6850 AleI CACnn'nnGTG Cuts at: 1166 4022 1166 Size: 2856 3994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6850 AlwNI CAG_nnn'CTG Cuts at: 2636 4593 6165 2636 Size: 1957 1572 3321 Fragments arranged by size: 3321 1957 1572 ApaLI G'TGCA_C Cuts at: 2859 4907 6063 2859 Size: 2048 1156 3646 Fragments arranged by size: 3646 2048 1156 ApoI r'AATT_y Cuts at: 1493 2135 2263 2823 3914 4159 4385 4537 6646 Size: 642 128 560 1091 245 226 152 2109 Cuts at: 6646 1493 Size: 1697 Fragments arranged by size: 2109 1697 1091 642 560 245 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 2057 2328 5404 160 Size: 872 1025 271 3076 1606 Fragments arranged by size: 3076 1606 1025 872 271 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 AvaI C'yCGr_G Cuts at: 1870 1943 2268 3077 3954 4175 1870 Size: 73 325 809 877 221 4545 Fragments arranged by size: 4545 877 809 325 221 73 BamHI G'GATC_C Cuts at: 4180 4547 4180 Size: 367 6483 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2702 3313 4171 5626 618 Size: 325 217 627 915 611 858 1455 1842 Fragments arranged by size: 1842 1455 915 858 627 611 325 217 BanII G_rGCy'C Cuts at: 1391 2694 3607 3748 4169 1391 Size: 1303 913 141 421 4072 Fragments arranged by size: 4072 1303 913 421 141 BbeI G_GCGC'C Cuts at: 3317 3317 Size: 6850 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3555 3586 928 Size: 971 1656 31 4192 Fragments arranged by size: 4192 1656 971 31 BbvCI CC'TCA_GC Cuts at: 2669 2669 Size: 6850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2828 2862 3953 3987 5041 5075 1903 Size: 34 891 34 1091 34 1054 34 3678 Fragments arranged by size: 3678 1091 1054 891 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4742 5952 4742 Size: 1210 5640 BclI T'GATC_A Cuts at: 1548 1548 Size: 6850 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4080 5461 136 Size: 107 122 71 631 605 2408 1381 1525 Fragments arranged by size: 2408 1525 1381 631 605 122 107 71 BglII A'GATC_T Cuts at: 6845 6845 Size: 6850 BlpI GC'TnA_GC Cuts at: 4267 4267 Size: 6850 Bme1580I G_kGCm'C Cuts at: 1790 2541 2705 2863 4911 6067 1790 Size: 751 164 158 2048 1156 2573 Fragments arranged by size: 2573 2048 1156 751 164 158 BmgBI CAC'GTC Cuts at: 1318 2913 3667 3967 1318 Size: 1595 754 300 4201 Fragments arranged by size: 4201 1595 754 300 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2021 3299 4581 Size: 416 53 83 399 24 604 1278 1282 Cuts at: 4581 5539 442 Size: 958 1753 Fragments arranged by size: 1753 1282 1278 958 604 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6850 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2786 3438 3560 4085 4099 4114 4129 5510 Size: 1547 652 122 525 14 15 15 1381 Cuts at: 5510 1239 Size: 2579 Fragments arranged by size: 2579 1547 1381 652 525 122 15 15 14 Bpu10I CC'TnA_GC Cuts at: 2669 2688 3035 3128 2669 Size: 19 347 93 6391 Fragments arranged by size: 6391 347 93 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 4975 5840 6138 2627 Size: 2348 865 298 3339 Fragments arranged by size: 3339 2348 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2801 3220 3563 3991 5513 882 Size: 617 1302 419 343 428 1522 2219 Fragments arranged by size: 2219 1522 1302 617 428 419 343 BsaAI yAC'GTr Cuts at: 493 1204 3031 4641 493 Size: 711 1827 1610 2702 Fragments arranged by size: 2702 1827 1610 711 BsaBI GATnn'nnATC Cuts at: 6844 6844 Size: 6850 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3314 4657 5039 5674 Size: 53 83 186 936 1781 1343 382 635 Cuts at: 5674 275 Size: 1451 Fragments arranged by size: 1781 1451 1343 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3522 5283 5681 5955 6102 6513 3522 Size: 1761 398 274 147 411 3859 Fragments arranged by size: 3859 1761 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3092 3122 3217 3247 3464 Size: 30 165 30 1689 30 95 30 217 Cuts at: 3464 3494 3846 3876 4118 4148 1178 Size: 30 352 30 242 30 3880 Fragments arranged by size: 3880 1689 352 242 217 165 95 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2952 3548 4102 1186 Size: 1766 596 554 3934 Fragments arranged by size: 3934 1766 596 554 BseYI C'CCAG_C Cuts at: 2678 3040 3805 4089 6053 6549 2678 Size: 362 765 284 1964 496 2979 Fragments arranged by size: 2979 1964 765 496 362 284 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 3800 3884 1163 Size: 573 294 1770 84 4129 Fragments arranged by size: 4129 1770 573 294 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4300 5061 6089 1540 Size: 275 186 2299 761 1028 2301 Fragments arranged by size: 2301 2299 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2863 3036 3336 4911 4996 6067 2863 Size: 173 300 1575 85 1071 3646 Fragments arranged by size: 3646 1575 1071 300 173 85 BsmI GAATG_Cn' Cuts at: 4361 4454 4361 Size: 93 6757 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2541 2694 2705 2863 3036 3336 3607 Size: 399 751 153 11 158 173 300 271 Cuts at: 3607 3748 4169 4911 4996 6067 1391 Size: 141 421 742 85 1071 2174 Fragments arranged by size: 2174 1071 751 742 421 399 300 271 173 158 153 141 85 11 BspEI T'CCGG_A Cuts at: 6513 6513 Size: 6850 BspHI T'CATG_A Cuts at: 4739 6469 4739 Size: 1730 5120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 3973 4205 844 Size: 2347 782 232 3489 Fragments arranged by size: 3489 2347 782 232 BsrBI CCG'CTC Cuts at: 1394 1823 4737 1394 Size: 429 2914 3507 Fragments arranged by size: 3507 2914 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 2454 5345 5519 65 Size: 1183 1087 119 2891 174 1396 Fragments arranged by size: 2891 1396 1183 1087 174 119 BsrFI r'CCGG_y Cuts at: 1700 1810 5494 5681 1700 Size: 110 3684 187 2869 Fragments arranged by size: 3684 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2037 96 Size: 1941 4909 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6850 BssSI C'ACGA_G Cuts at: 4910 5922 4910 Size: 1012 5838 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6850 BstBI TT'CG_AA Cuts at: 5654 5654 Size: 6850 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6850 BstXI CCAn_nnnn'nTGG Cuts at: 3064 3416 3064 Size: 352 6498 BstYI r'GATC_y Cuts at: 1972 4180 4547 4932 4949 6401 6487 6499 6845 Size: 2208 367 385 17 1452 86 12 346 Cuts at: 6845 1972 Size: 1977 Fragments arranged by size: 2208 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2964 2964 Size: 6850 BtgI C'CryG_G Cuts at: 513 1065 2004 2613 2694 513 Size: 552 939 609 81 4669 Fragments arranged by size: 4669 939 609 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3148 487 Size: 683 56 1922 4189 Fragments arranged by size: 4189 1922 683 56 BtsI GCAGTG_nn' Cuts at: 2125 3914 4366 5160 5180 2125 Size: 1789 452 794 20 3795 Fragments arranged by size: 3795 1789 794 452 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6815 DraI TTT'AAA Cuts at: 4154 4154 Size: 6850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5857 809 Size: 507 4541 1802 Fragments arranged by size: 4541 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 3887 4297 Size: 54 1425 50 275 18 786 1271 410 Cuts at: 4297 5186 6815 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1271 889 786 410 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4297 1537 Size: 275 2485 4090 Fragments arranged by size: 4090 2485 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2575 3135 3994 4780 1353 Size: 1222 560 859 786 3423 Fragments arranged by size: 3423 1222 859 786 560 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2541 5437 5811 5957 387 Size: 1064 138 952 2896 374 146 1280 Fragments arranged by size: 2896 1280 1064 952 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2556 2786 2957 3438 3560 3575 Size: 393 599 325 230 171 481 122 15 Cuts at: 3575 4085 4099 4114 4129 4913 5510 6297 6565 Size: 510 14 15 15 784 597 787 268 Cuts at: 6565 1239 Size: 1524 Fragments arranged by size: 1524 787 784 599 597 510 481 393 325 268 230 171 122 15 15 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 2074 2645 2074 Size: 571 6279 EcoO109I rG'GnC_Cy Cuts at: 2968 3413 2968 Size: 445 6405 EcoRI G'AATT_C Cuts at: 2135 2263 4159 4537 2135 Size: 128 1896 378 4448 Fragments arranged by size: 4448 1896 378 128 FspI TGC'GCA Cuts at: 5356 5356 Size: 6850 HaeII r_GCGC'y Cuts at: 1678 2179 3077 3245 3317 5997 1678 Size: 501 898 168 72 2680 2531 Fragments arranged by size: 2680 2531 898 501 168 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2425 2884 2916 2947 Size: 32 135 32 821 32 459 32 31 Cuts at: 2947 2979 3090 3122 3626 3658 5491 5523 5565 Size: 32 111 32 504 32 1833 32 42 Cuts at: 5565 5597 1373 Size: 32 2626 Fragments arranged by size: 2626 1833 821 504 459 135 111 42 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 4194 4440 669 Size: 988 281 2256 246 3079 Fragments arranged by size: 3079 2256 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6850 HpaI GTT'AAC Cuts at: 4440 4440 Size: 6850 KasI G'GCGC_C Cuts at: 3313 3313 Size: 6850 KpnI G_GTAC'C Cuts at: 4175 4175 Size: 6850 MfeI C'AATT_G Cuts at: 4449 4449 Size: 6850 MluI A'CGCG_T Cuts at: 6726 6726 Size: 6850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2525 5964 6148 1305 Size: 1220 3439 184 2007 Fragments arranged by size: 3439 2007 1220 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5028 Fragments arranged by size: 5028 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3260 4022 4808 5167 5326 518 Size: 648 2094 762 786 359 159 2042 Fragments arranged by size: 2094 2042 786 762 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2764 3805 4080 4093 4266 4943 6091 6336 Size: 1158 1041 275 13 173 677 1148 245 Cuts at: 6336 1606 Size: 2120 Fragments arranged by size: 2120 1158 1148 1041 677 275 245 173 13 NarI GG'CG_CC Cuts at: 3314 3314 Size: 6850 NcoI C'CATG_G Cuts at: 513 1065 2004 2613 513 Size: 552 939 609 4750 Fragments arranged by size: 4750 939 609 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6850 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6850 NotI GC'GGCC_GC Cuts at: 4297 4297 Size: 6850 NspI r_CATG'y Cuts at: 1132 1229 2094 3214 3481 3928 4208 5753 1132 Size: 97 865 1120 267 447 280 1545 2229 Fragments arranged by size: 2229 1545 1120 865 447 280 267 97 PasI CC'CwG_GG Cuts at: 1348 3318 3739 1348 Size: 1970 421 4459 Fragments arranged by size: 4459 1970 421 PciI A'CATG_T Cuts at: 2090 5749 2090 Size: 3659 3191 PflMI CCAn_nnn'nTGG Cuts at: 1108 3497 1108 Size: 2389 4461 PfoI T'CCnGG_A Cuts at: 2802 3415 2802 Size: 613 6237 PmeI GTTT'AAAC Cuts at: 4154 4154 Size: 6850 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4919 4951 6432 6464 4919 Size: 32 1481 32 5305 Fragments arranged by size: 5305 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2968 3413 2968 Size: 445 6405 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6850 PsiI TTA'TAA Cuts at: 2298 4420 2298 Size: 2122 4728 PstI C_TGCA'G Cuts at: 830 2769 3532 3909 3984 4202 830 Size: 1939 763 377 75 218 3478 Fragments arranged by size: 3478 1939 763 377 218 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6850 PvuII CAG'CTG Cuts at: 3805 4093 3805 Size: 288 6562 SalI G'TCGA_C Cuts at: 1936 4192 1936 Size: 2256 4594 SbfI CC_TGCA'GG Cuts at: 3984 4202 3984 Size: 218 6632 ScaI AGT'ACT Cuts at: 5098 6810 5098 Size: 1712 5138 SexAI A'CCwGG_T Cuts at: 3229 3881 3229 Size: 652 6198 SfcI C'TryA_G Cuts at: 826 1045 2765 3528 3817 3905 3980 4198 5333 Size: 219 1720 763 289 88 75 218 1135 Cuts at: 5333 6014 6205 826 Size: 681 191 1471 Fragments arranged by size: 1720 1471 1135 763 681 289 219 218 191 88 75 SfoI GGC'GCC Cuts at: 3315 3315 Size: 6850 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6850 SmaI CCC'GGG Cuts at: 1872 3956 4177 1872 Size: 2084 221 4545 Fragments arranged by size: 4545 2084 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 2642 4954 5855 6117 820 Size: 197 926 699 2312 901 262 1553 Fragments arranged by size: 2312 1553 926 901 699 262 197 SnaBI TAC'GTA Cuts at: 493 4641 493 Size: 4148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6850 SphI G_CATG'C Cuts at: 1132 3928 4208 1132 Size: 2796 280 3774 Fragments arranged by size: 3774 2796 280 SspI AAT'ATT Cuts at: 5 52 2875 4774 5 Size: 47 2823 1899 2081 Fragments arranged by size: 2823 2081 1899 47 StuI AGG'CCT Cuts at: 1517 3423 1517 Size: 1906 4944 StyI C'CwwG_G Cuts at: 513 1065 2004 2613 4289 513 Size: 552 939 609 1676 3074 Fragments arranged by size: 3074 1676 939 609 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2215 3739 4877 4894 5047 5232 5677 Size: 1255 635 1524 1138 17 153 185 445 Cuts at: 5677 325 Size: 1498 Fragments arranged by size: 1524 1498 1255 1138 635 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2037 2429 4011 Size: 274 80 33 51 1443 60 392 1582 Cuts at: 4011 5096 6808 96 Size: 1085 1712 138 Fragments arranged by size: 1712 1582 1443 1085 392 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3217 3249 3464 3496 3846 3878 4118 4150 6810 Size: 32 215 32 350 32 240 32 2660 Cuts at: 6810 6842 3217 Size: 32 3225 Fragments arranged by size: 3225 2660 350 240 215 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3794 1316 Size: 2478 4372 XbaI T'CTAG_A Cuts at: 4186 4186 Size: 6850 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4060 4060 Size: 6850 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6850 XmaI C'CCGG_G Cuts at: 1870 3954 4175 1870 Size: 2084 221 4545 Fragments arranged by size: 4545 2084 221 XmnI GAAnn'nnTTC Cuts at: 2135 4979 2135 Size: 2844 4006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4658 276 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI