(Circular) (Six-base) MAPSORT of: pFN21AB3039.seq Check: 319 from: 1 to: 8077 pFN21AB3039 8077 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2141 2544 2141 Size: 403 7674 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5887 278 Size: 53 83 186 936 4351 2468 Fragments arranged by size: 4351 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5420 1937 Size: 3483 4594 Acc65I G'GTAC_C Cuts at: 5398 5398 Size: 8077 AclI AA'CG_TT Cuts at: 1563 6204 6577 1563 Size: 4641 373 3063 Fragments arranged by size: 4641 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5385 6140 7524 7792 1632 Size: 3753 755 1384 268 1917 Fragments arranged by size: 3753 1917 1384 755 268 AflII C'TTAA_G Cuts at: 820 1017 4520 820 Size: 197 3503 4377 Fragments arranged by size: 4377 3503 197 AflIII A'CryG_T Cuts at: 1203 2753 2778 6976 7953 1203 Size: 1550 25 4198 977 1327 Fragments arranged by size: 4198 1550 1327 977 25 AgeI A'CCGG_T Cuts at: 6908 6908 Size: 8077 AhdI GACnn_n'nnGTC Cuts at: 6806 6806 Size: 8077 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8077 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8077 AlwNI CAG_nnn'CTG Cuts at: 3293 5820 7392 3293 Size: 2527 1572 3978 Fragments arranged by size: 3978 2527 1572 ApaLI G'TGCA_C Cuts at: 6134 7290 6134 Size: 1156 6921 ApoI r'AATT_y Cuts at: 1493 3098 3682 4478 5386 5612 5764 7873 1493 Size: 1605 584 796 908 226 152 2109 1697 Fragments arranged by size: 2109 1697 1605 908 796 584 226 152 AseI AT'TA_AT Cuts at: 160 1032 6631 160 Size: 872 5599 1606 Fragments arranged by size: 5599 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8077 AvaI C'yCGr_G Cuts at: 1870 1943 4851 4962 5402 1870 Size: 73 2908 111 440 4545 Fragments arranged by size: 4545 2908 440 111 73 BamHI G'GATC_C Cuts at: 2212 3243 5407 5774 2212 Size: 1031 2164 367 4515 Fragments arranged by size: 4515 2164 1031 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2151 5398 6853 618 Size: 325 217 627 282 82 3247 1455 1842 Fragments arranged by size: 3247 1842 1455 627 325 282 217 82 BanII G_rGCy'C Cuts at: 1391 2166 3018 5396 1391 Size: 775 852 2378 4072 Fragments arranged by size: 4072 2378 852 775 BbeI G_GCGC'C Cuts at: 2155 2155 Size: 8077 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4053 928 Size: 971 2154 4952 Fragments arranged by size: 4952 2154 971 BbvCI CC'TCA_GC Cuts at: 3964 3964 Size: 8077 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6268 6302 1903 Size: 34 4331 34 3678 Fragments arranged by size: 4331 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2334 5969 7179 2334 Size: 3635 1210 3232 Fragments arranged by size: 3635 3232 1210 BclI T'GATC_A Cuts at: 1548 2088 3763 4305 4597 1548 Size: 540 1675 542 292 5028 Fragments arranged by size: 5028 1675 542 540 292 BfrBI ATG'CAT Cuts at: 2724 2724 Size: 8077 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4847 6688 136 Size: 107 122 71 631 605 3175 1841 1525 Fragments arranged by size: 3175 1841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3886 8072 3886 Size: 4186 3891 BlpI GC'TnA_GC Cuts at: 2547 5494 2547 Size: 2947 5130 Bme1580I G_kGCm'C Cuts at: 1790 2502 6138 7294 1790 Size: 712 3636 1156 2573 Fragments arranged by size: 3636 2573 1156 712 BmgBI CAC'GTC Cuts at: 1318 2754 4846 1318 Size: 1436 2092 4549 Fragments arranged by size: 4549 2092 1436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5000 5808 6766 Size: 416 53 83 399 24 3583 808 958 Cuts at: 6766 442 Size: 1753 Fragments arranged by size: 3583 1753 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8077 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2612 2612 Size: 8077 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2583 2736 2985 3002 4784 5195 6737 1239 Size: 1344 153 249 17 1782 411 1542 2579 Fragments arranged by size: 2579 1782 1542 1344 411 249 153 17 Bpu10I CC'TnA_GC Cuts at: 2942 3964 5104 2942 Size: 1022 1140 5915 Fragments arranged by size: 5915 1140 1022 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2214 4085 4194 4888 6202 7067 7365 2214 Size: 1871 109 694 1314 865 298 2926 Fragments arranged by size: 2926 1871 1314 865 694 298 109 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6740 882 Size: 617 5241 2219 Fragments arranged by size: 5241 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3838 5311 5868 493 Size: 711 2634 1473 557 2702 Fragments arranged by size: 2702 2634 1473 711 557 BsaBI GATnn'nnATC Cuts at: 2087 5021 8071 2087 Size: 2934 3050 2093 Fragments arranged by size: 3050 2934 2093 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2152 5884 6266 6901 Size: 53 83 186 936 619 3732 382 635 Cuts at: 6901 275 Size: 1451 Fragments arranged by size: 3732 1451 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2628 3240 6510 6908 7182 7329 7740 2628 Size: 612 3270 398 274 147 411 2965 Fragments arranged by size: 3270 2965 612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2403 2433 3255 3285 3731 Size: 30 165 30 1000 30 822 30 446 Cuts at: 3731 3761 1178 Size: 30 5494 Fragments arranged by size: 5494 1000 822 446 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2340 3627 3912 3952 5309 1186 Size: 1154 1287 285 40 1357 3954 Fragments arranged by size: 3954 1357 1287 1154 285 40 BseYI C'CCAG_C Cuts at: 3293 4171 4774 4818 7280 7776 3293 Size: 878 603 44 2462 496 3594 Fragments arranged by size: 3594 2462 878 603 496 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2759 2827 2968 4406 5157 1163 Size: 573 1023 68 141 1438 751 4083 Fragments arranged by size: 4083 1438 1023 751 573 141 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5376 5527 6288 7316 1540 Size: 275 186 3375 151 761 1028 2301 Fragments arranged by size: 3375 2301 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 3183 6138 6223 7294 3183 Size: 2955 85 1071 3966 Fragments arranged by size: 3966 2955 1071 85 BsmI GAATG_Cn' Cuts at: 2666 2726 3355 4081 5588 5681 2666 Size: 60 629 726 1507 93 5062 Fragments arranged by size: 5062 1507 726 629 93 60 BsmBI CGTCTCn'nnnn_ Cuts at: 3598 4760 3598 Size: 1162 6915 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2502 3018 3183 5396 6138 6223 Size: 399 376 336 516 165 2213 742 85 Cuts at: 6223 7294 1391 Size: 1071 2174 Fragments arranged by size: 2213 2174 1071 742 516 399 376 336 165 85 BspEI T'CCGG_A Cuts at: 2628 7740 2628 Size: 5112 2965 BspHI T'CATG_A Cuts at: 4183 5966 7696 4183 Size: 1783 1730 4564 Fragments arranged by size: 4564 1783 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2141 2544 4286 5432 844 Size: 1297 403 1742 1146 3489 Fragments arranged by size: 3489 1742 1297 1146 403 BsrBI CCG'CTC Cuts at: 1394 1823 5964 1394 Size: 429 4141 3507 Fragments arranged by size: 4141 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2136 2905 4785 6572 6746 65 Size: 1183 888 769 1880 1787 174 1396 Fragments arranged by size: 1880 1787 1396 1183 888 769 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6721 6908 1700 Size: 110 4911 187 2869 Fragments arranged by size: 4911 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5046 96 Size: 4950 3127 BssHII G'CGCG_C Cuts at: 1925 4526 1925 Size: 2601 5476 BssSI C'ACGA_G Cuts at: 6137 7149 6137 Size: 1012 7065 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8077 BstBI TT'CG_AA Cuts at: 6881 6881 Size: 8077 BstEII G'GTnAC_C Cuts at: 1184 4192 1184 Size: 3008 5069 BstXI CCAn_nnnn'nTGG Cuts at: 2561 5013 2561 Size: 2452 5625 BstYI r'GATC_y Cuts at: 1972 2212 3243 3886 4438 4894 5407 5774 6159 Size: 240 1031 643 552 456 513 367 385 Cuts at: 6159 6176 7628 7714 7726 8072 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1031 643 552 513 456 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 4723 513 Size: 552 939 69 2650 3867 Fragments arranged by size: 3867 2650 939 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7338 Fragments arranged by size: 7338 683 56 BtsI GCAGTG_nn' Cuts at: 2469 2815 2900 3283 3891 5593 6387 6407 2469 Size: 346 85 383 608 1702 794 20 4139 Fragments arranged by size: 4139 1702 794 608 383 346 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3042 3077 529 Size: 35 2478 35 5529 Fragments arranged by size: 5529 2478 35 35 DraI TTT'AAA Cuts at: 5381 5381 Size: 8077 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7084 809 Size: 507 5768 1802 Fragments arranged by size: 5768 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 3128 4510 Size: 54 1425 50 275 18 744 554 1382 Cuts at: 4510 4704 5019 5373 5524 6413 8042 8 Size: 194 315 354 151 889 1629 43 Fragments arranged by size: 1629 1425 1382 889 744 554 354 315 275 194 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5373 5524 1537 Size: 275 3561 151 4090 Fragments arranged by size: 4090 3561 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2603 4316 6007 1353 Size: 911 339 1713 1691 3423 Fragments arranged by size: 3423 1713 1691 911 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6664 7038 7184 387 Size: 1064 138 5075 374 146 1280 Fragments arranged by size: 5075 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2583 2736 2985 3002 4784 5195 5385 Size: 393 951 153 249 17 1782 411 190 Cuts at: 5385 6140 6737 7524 7792 1239 Size: 755 597 787 268 1524 Fragments arranged by size: 1782 1524 951 787 755 597 411 393 268 249 190 153 17 EcoNI CCTnn'n_nnAGG Cuts at: 2614 3258 3642 2614 Size: 644 384 7049 Fragments arranged by size: 7049 644 384 EcoO109I rG'GnC_Cy Cuts at: 2654 4105 2654 Size: 1451 6626 EcoRI G'AATT_C Cuts at: 5386 5764 5386 Size: 378 7699 FspI TGC'GCA Cuts at: 6583 6583 Size: 8077 HaeII r_GCGC'y Cuts at: 1678 2155 7224 1678 Size: 477 5069 2531 Fragments arranged by size: 5069 2531 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2319 2351 2959 2991 3200 Size: 32 135 32 747 32 608 32 209 Cuts at: 3200 3232 3255 3287 3731 3763 3837 3869 3871 Size: 32 23 32 444 32 74 32 2 Cuts at: 3871 3903 5054 5086 5237 5269 6718 6750 6792 Size: 32 1151 32 151 32 1449 32 42 Cuts at: 6792 6824 1373 Size: 32 2626 Fragments arranged by size: 2626 1449 1151 747 608 444 209 151 135 74 42 32 32 32 32 32 32 32 32 32 32 32 32 32 23 2 HincII GTy'rAC Cuts at: 669 1657 1938 2363 3856 5421 5667 669 Size: 988 281 425 1493 1565 246 3079 Fragments arranged by size: 3079 1565 1493 988 425 281 246 HindIII A'AGCT_T Cuts at: 748 2367 2939 3117 3443 748 Size: 1619 572 178 326 5382 Fragments arranged by size: 5382 1619 572 326 178 HpaI GTT'AAC Cuts at: 5667 5667 Size: 8077 KasI G'GCGC_C Cuts at: 2151 2151 Size: 8077 KpnI G_GTAC'C Cuts at: 5402 5402 Size: 8077 MfeI C'AATT_G Cuts at: 4316 5676 4316 Size: 1360 6717 MluI A'CGCG_T Cuts at: 7953 7953 Size: 8077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3972 7191 7375 1305 Size: 2667 3219 184 2007 Fragments arranged by size: 3219 2667 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4512 4706 5021 10 Size: 54 1425 343 2680 194 315 3066 Fragments arranged by size: 3066 2680 1425 343 315 194 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 4059 6035 6394 6553 518 Size: 648 1188 1705 1976 359 159 2042 Fragments arranged by size: 2042 1976 1705 1188 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2218 2952 3968 4220 4382 5493 6170 7318 Size: 612 734 1016 252 162 1111 677 1148 Cuts at: 7318 7563 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1111 1016 734 677 612 252 245 162 NarI GG'CG_CC Cuts at: 2152 2152 Size: 8077 NcoI C'CATG_G Cuts at: 513 1065 2004 2073 4723 513 Size: 552 939 69 2650 3867 Fragments arranged by size: 3867 2650 939 552 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 8077 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8077 NotI GC'GGCC_GC Cuts at: 5524 5524 Size: 8077 NsiI A_TGCA'T Cuts at: 2726 2726 Size: 8077 NspI r_CATG'y Cuts at: 1132 1229 2910 4680 5435 6980 1132 Size: 97 1681 1770 755 1545 2229 Fragments arranged by size: 2229 1770 1681 1545 755 97 PasI CC'CwG_GG Cuts at: 1348 3825 3868 5161 1348 Size: 2477 43 1293 4264 Fragments arranged by size: 4264 2477 1293 43 PciI A'CATG_T Cuts at: 6976 6976 Size: 8077 PflMI CCAn_nnn'nTGG Cuts at: 1108 2247 2880 5269 1108 Size: 1139 633 2389 3916 Fragments arranged by size: 3916 2389 1139 633 PfoI T'CCnGG_A Cuts at: 3001 3592 3001 Size: 591 7486 PmeI GTTT'AAAC Cuts at: 5381 5381 Size: 8077 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6146 6178 7659 7691 6146 Size: 32 1481 32 6532 Fragments arranged by size: 6532 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2654 2654 Size: 8077 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8077 PsiI TTA'TAA Cuts at: 5647 5647 Size: 8077 PstI C_TGCA'G Cuts at: 830 2895 3278 5429 830 Size: 2065 383 2151 3478 Fragments arranged by size: 3478 2151 2065 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8077 PvuII CAG'CTG Cuts at: 2952 3968 4382 2952 Size: 1016 414 6647 Fragments arranged by size: 6647 1016 414 SalI G'TCGA_C Cuts at: 1936 5419 1936 Size: 3483 4594 SbfI CC_TGCA'GG Cuts at: 5429 5429 Size: 8077 ScaI AGT'ACT Cuts at: 6325 8037 6325 Size: 1712 6365 SexAI A'CCwGG_T Cuts at: 4128 4128 Size: 8077 SfcI C'TryA_G Cuts at: 826 1045 2891 3274 5167 5425 6560 7241 7432 Size: 219 1846 383 1893 258 1135 681 191 Cuts at: 7432 826 Size: 1471 Fragments arranged by size: 1893 1846 1471 1135 681 383 258 219 191 SfoI GGC'GCC Cuts at: 2153 2153 Size: 8077 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8077 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8077 SmaI CCC'GGG Cuts at: 1872 4853 5404 1872 Size: 2981 551 4545 Fragments arranged by size: 4545 2981 551 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 4100 4209 4520 4867 6181 Size: 197 926 286 1871 109 311 347 1314 Cuts at: 6181 7082 7344 820 Size: 901 262 1553 Fragments arranged by size: 1871 1553 1314 926 901 347 311 286 262 197 109 SnaBI TAC'GTA Cuts at: 493 5868 493 Size: 5375 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8077 SphI G_CATG'C Cuts at: 1132 2910 5435 1132 Size: 1778 2525 3774 Fragments arranged by size: 3774 2525 1778 SspI AAT'ATT Cuts at: 5 52 3702 3775 3987 6001 5 Size: 47 3650 73 212 2014 2081 Fragments arranged by size: 3650 2081 2014 212 73 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8077 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2983 3861 4258 4723 5516 Size: 552 939 69 910 878 397 465 793 Cuts at: 5516 513 Size: 3074 Fragments arranged by size: 3074 939 910 878 793 552 465 397 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2136 2850 6104 6121 6274 6459 6904 Size: 1255 556 714 3254 17 153 185 445 Cuts at: 6904 325 Size: 1498 Fragments arranged by size: 3254 1498 1255 714 556 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2530 2568 2675 Size: 274 80 33 51 1443 553 38 107 Cuts at: 2675 5046 6323 8035 96 Size: 2371 1277 1712 138 Fragments arranged by size: 2371 1712 1443 1277 553 274 138 107 80 51 38 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 3892 3924 5119 5151 8037 8069 2403 Size: 32 1457 32 1195 32 2886 32 2411 Fragments arranged by size: 2886 2411 1457 1195 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8077 XbaI T'CTAG_A Cuts at: 2524 5413 2524 Size: 2889 5188 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2711 4461 2711 Size: 1750 6327 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8077 XmaI C'CCGG_G Cuts at: 1870 4851 5402 1870 Size: 2981 551 4545 Fragments arranged by size: 4545 2981 551 XmnI GAAnn'nnTTC Cuts at: 6206 6206 Size: 8077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5885 276 Size: 53 83 186 936 4351 2468 Fragments arranged by size: 4351 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI