Restriction map for
pFN21AB1389
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BciVI BclI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrDI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HinfI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SpeI SphI Sse8387I
SspI SwaI TfiI Tsp45I Tsp509I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 695 ACACGT A^C[GA][CT]GT
AscI 153 GGCGCGCC GG^CGCGCC
Asp700I 609 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1607 TGCGCA TGC^GCA
BcgI 374 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1020 TACGTA [CT]AC^GT[GA]
BsiEI 1091 CGGCCG CG[GA][CT]^CG
BsmAI 1933 GAGAC GTCTC(1/5)
Bsp1407I 1631 TGTACA T^GTACA
BspMI 410 ACCTGC ACCTGC(4/8)
BsrGI 1631 TGTACA T^GTACA
BstAPI 546 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1862 GGATCT [GA]^GATC[CT]
DraII 243 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 896 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1798 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1091 CGGCCG C^GGCCG
EclXI 1091 CGGCCG C^GGCCG
Eco52I 1091 CGGCCG C^GGCCG
EcoO109I 243 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 911 CCAAGG C^C[AT][AT]GG
FspI 1607 TGCGCA TGC^GCA
HincII 136 GTTGAC GT[CT]^[GA]AC
HindII 136 GTTGAC GT[CT]^[GA]AC
MaeIII 1085 GTTAC ^GT[ACGT]AC
MflI 1862 GGATCT [GA]^GATC[CT]
NspI 1034 ACATGC [GA]CATG^[CT]
PshAI 1935 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 493 GAGCTC GAGCT^C
SfcI 1937 CTACAG C^T[GA][CT]AG
SnaBI 1020 TACGTA TAC^GTA
SspBI 1631 TGTACA T^GTACA
StuI 1679 AGGCCT AGG^CCT
StyI 911 CCAAGG C^C[AT][AT]GG
TaqI 1322 TCGA T^CGA
TthHB8I 1322 TCGA T^CGA
XhoII 1862 GGATCT [GA]^GATC[CT]
XmnI 609 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 1266 GATCC GGATC(4/5)
1862 GGATC
AlwNI 160 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
547 CAGGCGCTG
BalI 628 TGGCCA TGG^CCA
1244 TGGCCA
BfaI 469 CTAG C^TAG
541 CTAG
BlpI 490 GCTGAGC GC^T[ACGT]AGC
1419 GCTGAGC
Bpu1102I 490 GCTGAGC GC^T[ACGT]AGC
1419 GCTGAGC
BsgI 1206 CTGCAC GTGCAG(16/14)
478 GTGCAG
BssSI 1312 CACGAG CACGAG(-5/-1)
1905 CACGAG
BstXI 407 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
733 CCAGAGTTCTGG
CelII 490 GCTGAGC GC^T[ACGT]AGC
1419 GCTGAGC
MaeI 469 CTAG C^TAG
541 CTAG
MluNI 628 TGGCCA TGG^CCA
1244 TGGCCA
MscI 628 TGGCCA TGG^CCA
1244 TGGCCA
MseI 1445 TTAA T^TAA
1817 TTAA
MslI 1089 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1554 CACCATCATG
PflMI 867 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1253 CCATCTGGTGG
PvuII 160 CAGCTG CAG^CTG
1704 CAGCTG
SapI 1005 GCTCTTC GCTCTTC(1/4)
1539 GCTCTTC
SexAI 1025 ACCTGGT A^CC[AT]GGT
1279 ACCTGGT
Tru9I 1445 TTAA T^TAA
1817 TTAA
Van91I 867 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1253 CCATCTGGTGG
XcmI 1379 CCATCACCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1385 CCATCCTGGCGCTGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I ApaI ApaLI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvCI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspHI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI SgrAI SmaI
SmlI TseI TspRI XmaCI XmaI