Restriction map for
pFN21AB1389
(Linear) (Six-base) MAPSORT of: KIBB1389.seq Check: 287 from: 1 to: 1944
KIBB1389.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 1221 1605 1944
Size: 1029 192 384 339
Fragments arranged by size:
1029 384 339 192
AflIII A'CryG_T
Cuts at: 0 695 1944
Size: 695 1249
AleI CACnn'nnGTG
Cuts at: 0 1093 1944
Size: 1093 851
AlwNI CAG_nnn'CTG
Cuts at: 0 165 552 1944
Size: 165 387 1392
Fragments arranged by size:
1392 387 165
ApaI G_GGCC'C
Cuts at: 0 216 248 941 1944
Size: 216 32 693 1003
Fragments arranged by size:
1003 693 216 32
ApaLI G'TGCA_C
Cuts at: 0 372 451 531 661 1944
Size: 372 79 80 130 1283
Fragments arranged by size:
1283 372 130 80 79
AscI GG'CGCG_CC
Cuts at: 0 154 1944
Size: 154 1790
AvaI C'yCGr_G
Cuts at: 0 29 247 789 940 1944
Size: 29 218 542 151 1004
Fragments arranged by size:
1004 542 218 151 29
BanI G'GyrC_C
Cuts at: 0 15 667 709 773 796 846 1189 1479
Size: 15 652 42 64 23 50 343 290
Cuts at: 1479 1573 1768 1944
Size: 94 195 176
Fragments arranged by size:
652 343 290 195 176 94 64 50
42 23 15
BanII G_rGCy'C
Cuts at: 0 216 248 497 926 941 1795 1944
Size: 216 32 249 429 15 854 149
Fragments arranged by size:
854 429 249 216 149 32 15
BbeI G_GCGC'C
Cuts at: 0 19 671 800 1483 1772 1944
Size: 19 652 129 683 289 172
Fragments arranged by size:
683 652 289 172 129 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1136 1611 1800 1944
Size: 1136 475 189 144
Fragments arranged by size:
1136 475 189 144
BbvCI CC'TCA_GC
Cuts at: 0 5 281 755 1944
Size: 5 276 474 1189
Fragments arranged by size:
1189 474 276 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 363 397 1944
Size: 363 34 1547
Fragments arranged by size:
1547 363 34
BglI GCCn_nnn'nGGC
Cuts at: 0 88 220 1357 1944
Size: 88 132 1137 587
Fragments arranged by size:
1137 587 132 88
BlpI GC'TnA_GC
Cuts at: 0 491 1420 1944
Size: 491 929 524
Fragments arranged by size:
929 524 491
Bme1580I G_kGCm'C
Cuts at: 0 216 248 376 455 535 665 712 776
Size: 216 32 128 79 80 130 47 64
Cuts at: 776 851 941 1173 1192 1944
Size: 75 90 232 19 752
Fragments arranged by size:
752 232 216 130 128 90 80 79
75 64 47 32 19
BmgBI CAC'GTC
Cuts at: 0 698 1511 1850 1944
Size: 698 813 339 94
Fragments arranged by size:
813 698 339 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 466 1612 1738 1911 1944
Size: 205 261 1146 126 173 33
Fragments arranged by size:
1146 261 205 173 126 33
Bpu10I CC'TnA_GC
Cuts at: 0 5 281 755 1944
Size: 5 276 474 1189
Fragments arranged by size:
1189 474 276 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 625 826 1944
Size: 481 144 201 1118
Fragments arranged by size:
1118 481 201 144
BsaAI yAC'GTr
Cuts at: 0 1022 1944
Size: 1022 922
BsaHI Gr'CG_yC
Cuts at: 0 16 110 668 718 797 1480 1616 1769
Size: 16 94 558 50 79 683 136 153
Cuts at: 1769 1944
Size: 175
Fragments arranged by size:
683 558 175 153 136 94 79 50
16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 25 109 958 1944
Size: 22 3 84 849 986
Fragments arranged by size:
986 849 84 22 3
BseYI C'CCAG_C
Cuts at: 0 53 201 414 1395 1706 1944
Size: 53 148 213 981 311 238
Fragments arranged by size:
981 311 238 213 148 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1191 1944
Size: 499 692 753
Fragments arranged by size:
753 692 499
BsiEI CG_ry'CG
Cuts at: 0 1094 1944
Size: 1094 850
BsiHKAI G_wGCw'C
Cuts at: 0 376 455 497 514 535 665 1944
Size: 376 79 42 17 21 130 1279
Fragments arranged by size:
1279 376 130 79 42 21 17
Bsp1286I G_dGCh'C
Cuts at: 0 216 248 376 455 497 514 535 665
Size: 216 32 128 79 42 17 21 130
Cuts at: 665 712 776 851 926 941 1173 1192 1795
Size: 47 64 75 75 15 232 19 603
Cuts at: 1795 1944
Size: 149
Fragments arranged by size:
603 232 216 149 130 128 79 75
75 64 47 42 32 21 19 17
15
BspHI T'CATG_A
Cuts at: 0 605 1220 1559 1811 1944
Size: 605 615 339 252 133
Fragments arranged by size:
615 605 339 252 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 1944
Size: 419 1525
BsrBI CCG'CTC
Cuts at: 0 296 318 566 1059 1424 1944
Size: 296 22 248 493 365 520
Fragments arranged by size:
520 493 365 296 248 22
BsrFI r'CCGG_y
Cuts at: 0 37 112 670 799 1114 1365 1525 1944
Size: 37 75 558 129 315 251 160 419
Fragments arranged by size:
558 419 315 251 160 129 75 37
BsrGI T'GTAC_A
Cuts at: 0 1631 1944
Size: 1631 313
BssHII G'CGCG_C
Cuts at: 0 154 579 581 583 834 1944
Size: 154 425 2 2 251 1110
Fragments arranged by size:
1110 425 251 154 2 2
BssSI C'ACGA_G
Cuts at: 0 1312 1905 1944
Size: 1312 593 39
Fragments arranged by size:
1312 593 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 552 1944
Size: 552 1392
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 740 1944
Size: 414 326 1204
Fragments arranged by size:
1204 414 326
BstYI r'GATC_y
Cuts at: 0 1862 1944
Size: 1862 82
BtgI C'CryG_G
Cuts at: 0 339 777 1094 1100 1944
Size: 339 438 317 6 844
Fragments arranged by size:
844 438 339 317 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 366 1095 1427 1641 1944
Size: 366 729 332 214 303
Fragments arranged by size:
729 366 332 303 214
BtsI GCAGTG_nn'
Cuts at: 0 487 1944
Size: 487 1457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 328 363 1944
Size: 328 35 1581
Fragments arranged by size:
1581 328 35
DraIII CAC_nnn'GTG
Cuts at: 0 901 1944
Size: 901 1043
DrdI GACnn_nn'nnGTC
Cuts at: 0 1804 1944
Size: 1804 140
EaeI y'GGCC_r
Cuts at: 0 117 628 1091 1097 1244 1349 1367 1523
Size: 117 511 463 6 147 105 18 156
Cuts at: 1523 1944
Size: 421
Fragments arranged by size:
511 463 421 156 147 117 105 18
6
EagI C'GGCC_G
Cuts at: 0 1091 1944
Size: 1091 853
EarI CTCTTCn'nnn_
Cuts at: 0 1012 1222 1492 1546 1944
Size: 1012 210 270 54 398
Fragments arranged by size:
1012 398 270 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 782 1778 1944
Size: 106 676 996 166
Fragments arranged by size:
996 676 166 106
EcoICRI GAG'CTC
Cuts at: 0 495 1944
Size: 495 1449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 466 1029 1221 1605 1612 1738 1911
Size: 205 261 563 192 384 7 126 173
Cuts at: 1911 1944
Size: 33
Fragments arranged by size:
563 384 261 205 192 173 126 33
7
EcoO109I rG'GnC_Cy
Cuts at: 0 244 1944
Size: 244 1700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 636 1944
Size: 636 1308
FspI TGC'GCA
Cuts at: 0 1609 1944
Size: 1609 335
FspAI rTGC'GCAy
Cuts at: 0 1609 1944
Size: 1609 335
HaeII r_GCGC'y
Cuts at: 0 19 76 553 604 671 800 1044 1396
Size: 19 57 477 51 67 129 244 352
Cuts at: 1396 1483 1772 1944
Size: 87 289 172
Fragments arranged by size:
477 352 289 244 172 129 87 67
57 51 19
HincII GTy'rAC
Cuts at: 0 138 1944
Size: 138 1806
KasI G'GCGC_C
Cuts at: 0 15 667 796 1479 1768 1944
Size: 15 652 129 683 289 176
Fragments arranged by size:
683 652 289 176 129 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 788 1944
Size: 725 63 1156
Fragments arranged by size:
1156 725 63
MscI TGG'CCA
Cuts at: 0 630 1246 1944
Size: 630 616 698
Fragments arranged by size:
698 630 616
MslI CAynn'nnrTG
Cuts at: 0 1093 1558 1944
Size: 1093 465 386
Fragments arranged by size:
1093 465 386
MspA1I CmG'CkG
Cuts at: 0 104 162 195 369 490 1706 1944
Size: 104 58 33 174 121 1216 238
Fragments arranged by size:
1216 238 174 121 104 58 33
NaeI GCC'GGC
Cuts at: 0 39 672 801 1116 1367 1527 1944
Size: 39 633 129 315 251 160 417
Fragments arranged by size:
633 417 315 251 160 129 39
NarI GG'CG_CC
Cuts at: 0 16 668 797 1480 1769 1944
Size: 16 652 129 683 289 175
Fragments arranged by size:
683 652 289 175 129 16
NgoMIV G'CCGG_C
Cuts at: 0 37 670 799 1114 1365 1525 1944
Size: 37 633 129 315 251 160 419
Fragments arranged by size:
633 419 315 251 160 129 37
NspI r_CATG'y
Cuts at: 0 1038 1944
Size: 1038 906
PflMI CCAn_nnn'nTGG
Cuts at: 0 873 1259 1944
Size: 873 386 685
Fragments arranged by size:
873 685 386
PfoI T'CCnGG_A
Cuts at: 0 332 1124 1685 1888 1944
Size: 332 792 561 203 56
Fragments arranged by size:
792 561 332 203 56
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 484 516 1944
Size: 484 32 1428
Fragments arranged by size:
1428 484 32
PshAI GACnn'nnGTC
Cuts at: 0 1939 1944
Size: 1939 5
PspOMI G'GGCC_C
Cuts at: 0 212 244 937 1944
Size: 212 32 693 1007
Fragments arranged by size:
1007 693 212 32
PvuII CAG'CTG
Cuts at: 0 162 1706 1944
Size: 162 1544 238
Fragments arranged by size:
1544 238 162
SacI G_AGCT'C
Cuts at: 0 497 1944
Size: 497 1447
SapI GCTCTTCn'nnn_
Cuts at: 0 1012 1546 1944
Size: 1012 534 398
Fragments arranged by size:
1012 534 398
SexAI A'CCwGG_T
Cuts at: 0 1025 1279 1944
Size: 1025 254 665
Fragments arranged by size:
1025 665 254
SfcI C'TryA_G
Cuts at: 0 1937 1944
Size: 1937 7
SfoI GGC'GCC
Cuts at: 0 17 669 798 1481 1770 1944
Size: 17 652 129 683 289 174
Fragments arranged by size:
683 652 289 174 129 17
SgrAI Cr'CCGG_yG
Cuts at: 0 37 112 670 799 1944
Size: 37 75 558 129 1145
Fragments arranged by size:
1145 558 129 75 37
SmaI CCC'GGG
Cuts at: 0 31 249 791 1944
Size: 31 218 542 1153
Fragments arranged by size:
1153 542 218 31
SmlI C'TyrA_G
Cuts at: 0 496 640 841 1944
Size: 496 144 201 1103
Fragments arranged by size:
1103 496 201 144
SnaBI TAC'GTA
Cuts at: 0 1022 1944
Size: 1022 922
SrfI GCCC'GGGC
Cuts at: 0 249 1944
Size: 249 1695
StuI AGG'CCT
Cuts at: 0 1681 1944
Size: 1681 263
StyI C'CwwG_G
Cuts at: 0 911 1944
Size: 911 1033
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1148 1251 1597 1698 1944
Size: 1148 103 346 101 246
Fragments arranged by size:
1148 346 246 103 101
TatI w'GTAC_w
Cuts at: 0 305 929 1631 1944
Size: 305 624 702 313
Fragments arranged by size:
702 624 313 305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1386 1392 1944
Size: 1386 6 552
Fragments arranged by size:
1386 552 6
XmaI C'CCGG_G
Cuts at: 0 29 247 789 1944
Size: 29 218 542 1155
Fragments arranged by size:
1155 542 218 29
XmnI GAAnn'nnTTC
Cuts at: 0 613 1944
Size: 613 1331
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI AscI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI FspI FspAI HaeII
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PflMI PfoI PpiI PshAI PspOMI PvuII
SacI SapI SexAI SfcI SfoI SgrAI SmaI SmlI
SnaBI SrfI StuI StyI TaqII TatI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApoI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BmrI BmtI BplI
BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BsrDI BstBI BstEII BstZ17I Bsu36I ClaI DraI EcoNI
EcoRI EcoRV FseI Hin4I HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpuMI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SgfI SpeI SphI SspI SwaI TstI Tth111I
XbaI XhoI ZraI